(Linear) (Six-base) MAPSORT of: KIEE3508.seq Check: 2650 from: 1 to: 2109 KIEE3508.orf With 182 enzymes: SgfI * July 3, 2012 18:01 .. Acc65I G'GTAC_C Cuts at: 0 188 2109 Size: 188 1921 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1151 1940 2109 Size: 1151 789 169 Fragments arranged by size: 1151 789 169 AfeI AGC'GCT Cuts at: 0 1517 2109 Size: 1517 592 AflIII A'CryG_T Cuts at: 0 1571 2109 Size: 1571 538 AleI CACnn'nnGTG Cuts at: 0 1315 2109 Size: 1315 794 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 479 1411 2109 Size: 479 932 698 Fragments arranged by size: 932 698 479 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2075 2107 2109 Size: 2075 32 2 Fragments arranged by size: 2075 32 2 AlwNI CAG_nnn'CTG Cuts at: 0 64 378 1060 2109 Size: 64 314 682 1049 Fragments arranged by size: 1049 682 314 64 ApaI G_GGCC'C Cuts at: 0 928 1128 1816 2109 Size: 928 200 688 293 Fragments arranged by size: 928 688 293 200 AvaI C'yCGr_G Cuts at: 0 38 206 511 686 2109 Size: 38 168 305 175 1423 Fragments arranged by size: 1423 305 175 168 38 BanI G'GyrC_C Cuts at: 0 20 188 463 1597 1669 2011 2109 Size: 20 168 275 1134 72 342 98 Fragments arranged by size: 1134 342 275 168 98 72 20 BanII G_rGCy'C Cuts at: 0 152 626 769 875 928 1128 1816 1823 Size: 152 474 143 106 53 200 688 7 Cuts at: 1823 1958 2109 Size: 135 151 Fragments arranged by size: 688 474 200 152 151 143 135 106 53 7 BbvCI CC'TCA_GC Cuts at: 0 712 2109 Size: 712 1397 BciVI GTATCCnnnnn_n' Cuts at: 0 1331 2109 Size: 1331 778 BglI GCCn_nnn'nGGC Cuts at: 0 572 581 2109 Size: 572 9 1528 Fragments arranged by size: 1528 572 9 BlpI GC'TnA_GC Cuts at: 0 1741 2109 Size: 1741 368 Bme1580I G_kGCm'C Cuts at: 0 25 410 711 897 928 1128 1602 1816 Size: 25 385 301 186 31 200 474 214 Cuts at: 1816 2109 Size: 293 Fragments arranged by size: 474 385 301 293 214 200 186 31 25 BmrI ACTGGGnnnn_n' Cuts at: 0 1149 1351 2109 Size: 1149 202 758 Fragments arranged by size: 1149 758 202 BmtI G_CTAG'C Cuts at: 0 557 2109 Size: 557 1552 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1812 2109 Size: 1812 297 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 71 1006 1053 1258 1648 1807 1888 1981 Size: 71 935 47 205 390 159 81 93 Cuts at: 1981 2082 2109 Size: 101 27 Fragments arranged by size: 935 390 205 159 101 93 81 71 47 27 Bpu10I CC'TnA_GC Cuts at: 0 712 1817 2109 Size: 712 1105 292 Fragments arranged by size: 1105 712 292 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1678 1690 2109 Size: 1678 12 419 Fragments arranged by size: 1678 419 12 BsaI GGTCTCn'nnnn_ Cuts at: 0 214 219 1057 2109 Size: 214 5 838 1052 Fragments arranged by size: 1052 838 214 5 BsaAI yAC'GTr Cuts at: 0 293 405 2109 Size: 293 112 1704 Fragments arranged by size: 1704 293 112 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 157 160 1519 2109 Size: 157 3 1359 590 Fragments arranged by size: 1359 590 157 3 BseYI C'CCAG_C Cuts at: 0 30 875 1382 2109 Size: 30 845 507 727 Fragments arranged by size: 845 727 507 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 721 1473 1507 2109 Size: 721 752 34 602 Fragments arranged by size: 752 721 602 34 BsiEI CG_ry'CG Cuts at: 0 1547 1951 2109 Size: 1547 404 158 Fragments arranged by size: 1547 404 158 BsiHKAI G_wGCw'C Cuts at: 0 152 290 1841 1958 2109 Size: 152 138 1551 117 151 Fragments arranged by size: 1551 152 151 138 117 Bsp1286I G_dGCh'C Cuts at: 0 25 152 290 410 626 711 769 875 Size: 25 127 138 120 216 85 58 106 Cuts at: 875 897 928 1128 1602 1816 1823 1841 1958 Size: 22 31 200 474 214 7 18 117 Cuts at: 1958 2109 Size: 151 Fragments arranged by size: 474 216 214 200 151 138 127 120 117 106 85 58 31 25 22 18 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 743 1442 2094 2109 Size: 743 699 652 15 Fragments arranged by size: 743 699 652 15 BsrBI CCG'CTC Cuts at: 0 1509 2096 2109 Size: 1509 587 13 Fragments arranged by size: 1509 587 13 BsrDI GCAATG_nn' Cuts at: 0 835 2109 Size: 835 1274 BsrFI r'CCGG_y Cuts at: 0 1229 1415 1492 2109 Size: 1229 186 77 617 Fragments arranged by size: 1229 617 186 77 BssSI C'ACGA_G Cuts at: 0 415 2109 Size: 415 1694 BstAPI GCAn_nnn'nTGC Cuts at: 0 841 2109 Size: 841 1268 BstEII G'GTnAC_C Cuts at: 0 639 1641 2109 Size: 639 1002 468 Fragments arranged by size: 1002 639 468 BstXI CCAn_nnnn'nTGG Cuts at: 0 952 1467 1539 2109 Size: 952 515 72 570 Fragments arranged by size: 952 570 515 72 BstYI r'GATC_y Cuts at: 0 168 725 2109 Size: 168 557 1384 Fragments arranged by size: 1384 557 168 Bsu36I CC'TnA_GG Cuts at: 0 570 2109 Size: 570 1539 BtgI C'CryG_G Cuts at: 0 192 906 1410 1472 1893 2109 Size: 192 714 504 62 421 216 Fragments arranged by size: 714 504 421 216 192 62 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 98 665 2109 Size: 98 567 1444 Fragments arranged by size: 1444 567 98 BtsI GCAGTG_nn' Cuts at: 0 499 626 2060 2109 Size: 499 127 1434 49 Fragments arranged by size: 1434 499 127 49 DraIII CAC_nnn'GTG Cuts at: 0 1845 2109 Size: 1845 264 EaeI y'GGCC_r Cuts at: 0 195 1005 1193 1231 1413 1469 1544 1948 Size: 195 810 188 38 182 56 75 404 Cuts at: 1948 2109 Size: 161 Fragments arranged by size: 810 404 195 188 182 161 75 56 38 EagI C'GGCC_G Cuts at: 0 1544 1948 2109 Size: 1544 404 161 Fragments arranged by size: 1544 404 161 EcoICRI GAG'CTC Cuts at: 0 150 1956 2109 Size: 150 1806 153 Fragments arranged by size: 1806 153 150 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 71 1006 1053 1151 1258 1648 1807 1888 Size: 71 935 47 98 107 390 159 81 Cuts at: 1888 1940 1981 2082 2109 Size: 52 41 101 27 Fragments arranged by size: 935 390 159 107 101 98 81 71 52 47 41 27 EcoNI CCTnn'n_nnAGG Cuts at: 0 532 955 1717 2109 Size: 532 423 762 392 Fragments arranged by size: 762 532 423 392 EcoO109I rG'GnC_Cy Cuts at: 0 35 574 924 925 1124 1125 1812 1813 Size: 35 539 350 1 199 1 687 1 Cuts at: 1813 2055 2109 Size: 242 54 Fragments arranged by size: 687 539 350 242 199 54 35 1 1 1 FspI TGC'GCA Cuts at: 0 1255 2109 Size: 1255 854 HaeII r_GCGC'y Cuts at: 0 1519 2037 2109 Size: 1519 518 72 Fragments arranged by size: 1519 518 72 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 724 756 985 1017 2109 Size: 724 32 229 32 1092 Fragments arranged by size: 1092 724 229 32 32 KpnI G_GTAC'C Cuts at: 0 192 2109 Size: 192 1917 MscI TGG'CCA Cuts at: 0 1007 2109 Size: 1007 1102 MslI CAynn'nnrTG Cuts at: 0 1315 1913 2109 Size: 1315 598 196 Fragments arranged by size: 1315 598 196 MspA1I CmG'CkG Cuts at: 0 299 704 879 1382 1539 1740 2109 Size: 299 405 175 503 157 201 369 Fragments arranged by size: 503 405 369 299 201 175 157 NaeI GCC'GGC Cuts at: 0 1417 2109 Size: 1417 692 NcoI C'CATG_G Cuts at: 0 192 906 1410 2109 Size: 192 714 504 699 Fragments arranged by size: 714 699 504 192 NgoMIV G'CCGG_C Cuts at: 0 1415 2109 Size: 1415 694 NheI G'CTAG_C Cuts at: 0 553 2109 Size: 553 1556 NspI r_CATG'y Cuts at: 0 1309 1575 2109 Size: 1309 266 534 Fragments arranged by size: 1309 534 266 PasI CC'CwG_GG Cuts at: 0 1220 1823 2109 Size: 1220 603 286 Fragments arranged by size: 1220 603 286 PciI A'CATG_T Cuts at: 0 1571 2109 Size: 1571 538 PflMI CCAn_nnn'nTGG Cuts at: 0 64 2079 2109 Size: 64 2015 30 Fragments arranged by size: 2015 64 30 PfoI T'CCnGG_A Cuts at: 0 104 254 885 1958 2109 Size: 104 150 631 1073 151 Fragments arranged by size: 1073 631 151 150 104 PmlI CAC'GTG Cuts at: 0 293 405 2109 Size: 293 112 1704 Fragments arranged by size: 1704 293 112 PpuMI rG'GwC_Cy Cuts at: 0 35 2055 2109 Size: 35 2020 54 Fragments arranged by size: 2020 54 35 PspOMI G'GGCC_C Cuts at: 0 924 1124 1812 2109 Size: 924 200 688 297 Fragments arranged by size: 924 688 297 200 PspXI vC'TCGA_Gb Cuts at: 0 511 2109 Size: 511 1598 PstI C_TGCA'G Cuts at: 0 819 1934 2109 Size: 819 1115 175 Fragments arranged by size: 1115 819 175 PvuII CAG'CTG Cuts at: 0 704 879 1539 2109 Size: 704 175 660 570 Fragments arranged by size: 704 660 570 175 SacI G_AGCT'C Cuts at: 0 152 1958 2109 Size: 152 1806 151 Fragments arranged by size: 1806 152 151 SanDI GG'GwC_CC Cuts at: 0 35 2109 Size: 35 2074 SfcI C'TryA_G Cuts at: 0 783 815 987 1930 2109 Size: 783 32 172 943 179 Fragments arranged by size: 943 783 179 172 32 SmlI C'TyrA_G Cuts at: 0 511 1693 1705 2109 Size: 511 1182 12 404 Fragments arranged by size: 1182 511 404 12 StuI AGG'CCT Cuts at: 0 313 961 1901 2109 Size: 313 648 940 208 Fragments arranged by size: 940 648 313 208 StyI C'CwwG_G Cuts at: 0 89 192 224 578 906 951 1313 1352 Size: 89 103 32 354 328 45 362 39 Cuts at: 1352 1410 2109 Size: 58 699 Fragments arranged by size: 699 362 354 328 103 89 58 45 39 32 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 631 759 1585 1629 2109 Size: 631 128 826 44 480 Fragments arranged by size: 826 631 480 128 44 TatI w'GTAC_w Cuts at: 0 98 2109 Size: 98 2011 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1199 1231 1441 1473 1740 1772 2109 Size: 1199 32 210 32 267 32 337 Fragments arranged by size: 1199 337 267 210 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 637 2053 2109 Size: 637 1416 56 Fragments arranged by size: 1416 637 56 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 525 2109 Size: 525 1584 XhoI C'TCGA_G Cuts at: 0 511 2109 Size: 511 1598 Enzymes that do cut: Acc65I AcuI AfeI AflIII AleI AlfI AloI AlwNI ApaI AvaI BanI BanII BbvCI BciVI BglI BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KpnI MscI MslI MspA1I NaeI NcoI NgoMIV NheI NspI PasI PciI PflMI PfoI PmlI PpuMI PspOMI PspXI PstI PvuII SacI SanDI SfcI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI Enzymes that do not cut: AarI AatII AccI AclI AflII AgeI AhdI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BcgI BclI BfrBI BglII BmgBI BsaBI BsaHI BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII BstBI BstZ17I ClaI CspCI DraI DrdI EarI EciI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KasI MfeI MluI MmeI NarI NdeI NotI NruI NsiI PacI PmeI PpiI PshAI PsiI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI XbaI XmaI XmnI ZraI