(Circular) (Six-base) MAPSORT of: pFN21AB9954.seq Check: 2901 from: 1 to: 6736 pFN21AB9954 6736 bp With 182 enzymes: SgfI * April 15, 2010 09:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3844 3844 Size: 6736 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4546 278 Size: 53 83 186 936 3010 2468 Fragments arranged by size: 3010 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4079 1937 Size: 2142 4594 Acc65I G'GTAC_C Cuts at: 4057 4057 Size: 6736 AclI AA'CG_TT Cuts at: 1563 4863 5236 1563 Size: 3300 373 3063 Fragments arranged by size: 3300 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2169 2620 2921 3353 4799 6183 6451 1632 Size: 537 451 301 432 1446 1384 268 1917 Fragments arranged by size: 1917 1446 1384 537 451 432 301 268 AfeI AGC'GCT Cuts at: 2170 2170 Size: 6736 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6539 AflIII A'CryG_T Cuts at: 1203 5635 6612 1203 Size: 4432 977 1327 Fragments arranged by size: 4432 1327 977 AgeI A'CCGG_T Cuts at: 5567 5567 Size: 6736 AhdI GACnn_n'nnGTC Cuts at: 5465 5465 Size: 6736 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6736 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2232 4005 1184 Size: 1048 1773 3915 Fragments arranged by size: 3915 1773 1048 AlwNI CAG_nnn'CTG Cuts at: 4479 6051 4479 Size: 1572 5164 ApaLI G'TGCA_C Cuts at: 4793 5949 4793 Size: 1156 5580 ApoI r'AATT_y Cuts at: 1493 2332 2489 2684 2753 3033 3267 3355 3405 Size: 839 157 195 69 280 234 88 50 Cuts at: 3405 3412 4009 4045 4271 4423 6532 1493 Size: 7 597 36 226 152 2109 1697 Fragments arranged by size: 2109 1697 839 597 280 234 226 195 157 152 88 69 50 36 7 AseI AT'TA_AT Cuts at: 160 1032 5290 160 Size: 872 4258 1606 Fragments arranged by size: 4258 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6736 AvaI C'yCGr_G Cuts at: 1870 1943 4061 1870 Size: 73 2118 4545 Fragments arranged by size: 4545 2118 73 BamHI G'GATC_C Cuts at: 3800 4066 4433 3800 Size: 266 367 6103 Fragments arranged by size: 6103 367 266 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4057 5512 618 Size: 325 217 627 2270 1455 1842 Fragments arranged by size: 2270 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4055 1391 Size: 2664 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2019 2157 2789 3876 928 Size: 971 120 138 632 1087 3788 Fragments arranged by size: 3788 1087 971 632 138 120 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4927 4961 1903 Size: 34 2990 34 3678 Fragments arranged by size: 3678 2990 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3613 4628 5838 3613 Size: 1015 1210 4511 Fragments arranged by size: 4511 1210 1015 BclI T'GATC_A Cuts at: 1548 2998 3499 3814 1548 Size: 1450 501 315 4470 Fragments arranged by size: 4470 1450 501 315 BfrBI ATG'CAT Cuts at: 2735 2975 3010 2735 Size: 240 35 6461 Fragments arranged by size: 6461 240 35 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2109 2187 5347 Size: 107 122 71 631 605 437 78 3160 Cuts at: 5347 136 Size: 1525 Fragments arranged by size: 3160 1525 631 605 437 122 107 78 71 BglII A'GATC_T Cuts at: 3628 6731 3628 Size: 3103 3633 BlpI GC'TnA_GC Cuts at: 3276 4153 3276 Size: 877 5859 Bme1580I G_kGCm'C Cuts at: 1790 4797 5953 1790 Size: 3007 1156 2573 Fragments arranged by size: 3007 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2591 1318 Size: 1273 5463 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4467 5425 442 Size: 416 53 83 399 24 3050 958 1753 Fragments arranged by size: 3050 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6736 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2529 2529 Size: 6736 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5396 1239 Size: 4157 2579 Bpu10I CC'TnA_GC Cuts at: 2965 2965 Size: 6736 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2904 4861 5726 6024 2904 Size: 1957 865 298 3616 Fragments arranged by size: 3616 1957 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2265 5399 882 Size: 617 766 3134 2219 Fragments arranged by size: 3134 2219 766 617 BsaAI yAC'GTr Cuts at: 493 1204 4527 493 Size: 711 3323 2702 Fragments arranged by size: 3323 2702 711 BsaBI GATnn'nnATC Cuts at: 2895 6730 2895 Size: 3835 2901 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3196 4543 4925 5560 Size: 53 83 186 936 1663 1347 382 635 Cuts at: 5560 275 Size: 1451 Fragments arranged by size: 1663 1451 1347 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5169 5567 5841 5988 6399 5169 Size: 398 274 147 411 5506 Fragments arranged by size: 5506 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2607 2637 1178 Size: 30 165 30 1204 30 5277 Fragments arranged by size: 5277 1204 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6736 BseYI C'CCAG_C Cuts at: 2278 5939 6435 2278 Size: 3661 496 2579 Fragments arranged by size: 3661 2579 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3453 1163 Size: 573 1717 4446 Fragments arranged by size: 4446 1717 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4186 4947 5975 1540 Size: 275 186 2185 761 1028 2301 Fragments arranged by size: 2301 2185 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2416 3829 4797 4882 5953 2416 Size: 1413 968 85 1071 3199 Fragments arranged by size: 3199 1413 1071 968 85 BsmI GAATG_Cn' Cuts at: 4247 4340 4247 Size: 93 6643 BsmBI CGTCTCn'nnnn_ Cuts at: 2672 2672 Size: 6736 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2416 3829 4055 4797 4882 5953 1391 Size: 399 626 1413 226 742 85 1071 2174 Fragments arranged by size: 2174 1413 1071 742 626 399 226 85 BspEI T'CCGG_A Cuts at: 6399 6399 Size: 6736 BspHI T'CATG_A Cuts at: 2242 2656 2995 4625 6355 2242 Size: 414 339 1630 1730 2623 Fragments arranged by size: 2623 1730 1630 414 339 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3844 4091 844 Size: 3000 247 3489 Fragments arranged by size: 3489 3000 247 BsrBI CCG'CTC Cuts at: 1394 1823 2579 4623 1394 Size: 429 756 2044 3507 Fragments arranged by size: 3507 2044 756 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2197 3012 5231 5405 65 Size: 1183 949 815 2219 174 1396 Fragments arranged by size: 2219 1396 1183 949 815 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3198 5380 5567 1700 Size: 110 1388 2182 187 2869 Fragments arranged by size: 2869 2182 1388 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6736 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6736 BssSI C'ACGA_G Cuts at: 4796 5808 4796 Size: 1012 5724 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6736 BstBI TT'CG_AA Cuts at: 5540 5540 Size: 6736 BstEII G'GTnAC_C Cuts at: 1184 2570 1184 Size: 1386 5350 BstYI r'GATC_y Cuts at: 1972 3628 3800 4066 4433 4818 4835 6287 6373 Size: 1656 172 266 367 385 17 1452 86 Cuts at: 6373 6385 6731 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1656 1452 385 367 346 266 172 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2526 513 Size: 552 1461 4723 Fragments arranged by size: 4723 1461 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5997 Fragments arranged by size: 5997 683 56 BtsI GCAGTG_nn' Cuts at: 4252 5046 5066 4252 Size: 794 20 5922 Fragments arranged by size: 5922 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6736 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6701 DraI TTT'AAA Cuts at: 4006 4040 4006 Size: 34 6702 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5743 809 Size: 507 4427 1802 Fragments arranged by size: 4427 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4183 5072 6701 Size: 54 1425 50 275 18 2353 889 1629 Cuts at: 6701 8 Size: 43 Fragments arranged by size: 2353 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4183 1537 Size: 275 2371 4090 Fragments arranged by size: 4090 2371 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2704 2944 4666 1353 Size: 1351 240 1722 3423 Fragments arranged by size: 3423 1722 1351 240 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5323 5697 5843 387 Size: 1064 138 3734 374 146 1280 Fragments arranged by size: 3734 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2169 2620 2921 3353 4799 5396 6183 Size: 393 537 451 301 432 1446 597 787 Cuts at: 6183 6451 1239 Size: 268 1524 Fragments arranged by size: 1524 1446 787 597 537 451 432 393 301 268 EcoRI G'AATT_C Cuts at: 3355 3405 4045 4423 3355 Size: 50 640 378 5668 Fragments arranged by size: 5668 640 378 50 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3334 3334 Size: 6736 FspI TGC'GCA Cuts at: 5242 5242 Size: 6736 HaeII r_GCGC'y Cuts at: 1678 2172 5883 1678 Size: 494 3711 2531 Fragments arranged by size: 3711 2531 494 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3069 3101 3484 3516 3640 Size: 32 135 32 1497 32 383 32 124 Cuts at: 3640 3672 5377 5409 5451 5483 1373 Size: 32 1705 32 42 32 2626 Fragments arranged by size: 2626 1705 1497 383 135 124 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3234 3325 4080 4326 669 Size: 988 281 1296 91 755 246 3079 Fragments arranged by size: 3079 1296 988 755 281 246 91 HindIII A'AGCT_T Cuts at: 748 2046 748 Size: 1298 5438 HpaI GTT'AAC Cuts at: 3234 4326 3234 Size: 1092 5644 KpnI G_GTAC'C Cuts at: 4061 4061 Size: 6736 MfeI C'AATT_G Cuts at: 2913 4335 2913 Size: 1422 5314 MluI A'CGCG_T Cuts at: 6612 6612 Size: 6736 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2122 2939 3878 3978 5850 6034 1305 Size: 817 817 939 100 1872 184 2007 Fragments arranged by size: 2007 1872 939 817 817 184 100 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4914 Fragments arranged by size: 4914 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2214 2241 4694 5053 5212 518 Size: 648 1048 27 2453 359 159 2042 Fragments arranged by size: 2453 2042 1048 648 359 159 27 MspA1I CmG'CkG Cuts at: 1606 2278 2702 4152 4829 5977 6222 1606 Size: 672 424 1450 677 1148 245 2120 Fragments arranged by size: 2120 1450 1148 677 672 424 245 NaeI GCC'GGC Cuts at: 3200 3200 Size: 6736 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6184 NdeI CA'TA_TG Cuts at: 387 387 Size: 6736 NgoMIV G'CCGG_C Cuts at: 3198 3198 Size: 6736 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6736 NotI GC'GGCC_GC Cuts at: 4183 4183 Size: 6736 NsiI A_TGCA'T Cuts at: 2737 2977 3012 2737 Size: 240 35 6461 Fragments arranged by size: 6461 240 35 NspI r_CATG'y Cuts at: 1132 1229 2958 4094 5639 1132 Size: 97 1729 1136 1545 2229 Fragments arranged by size: 2229 1729 1545 1136 97 PasI CC'CwG_GG Cuts at: 1348 3898 1348 Size: 2550 4186 PciI A'CATG_T Cuts at: 5635 5635 Size: 6736 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6736 PmeI GTTT'AAAC Cuts at: 4040 4040 Size: 6736 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4805 4837 6318 6350 4805 Size: 32 1481 32 5191 Fragments arranged by size: 5191 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6736 PsiI TTA'TAA Cuts at: 4306 4306 Size: 6736 PstI C_TGCA'G Cuts at: 830 2134 2520 4088 830 Size: 1304 386 1568 3478 Fragments arranged by size: 3478 1568 1304 386 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6736 PvuII CAG'CTG Cuts at: 2278 2702 2278 Size: 424 6312 SalI G'TCGA_C Cuts at: 1936 4078 1936 Size: 2142 4594 SapI GCTCTTCn'nnn_ Cuts at: 2704 2944 2704 Size: 240 6496 SbfI CC_TGCA'GG Cuts at: 4088 4088 Size: 6736 ScaI AGT'ACT Cuts at: 4984 6696 4984 Size: 1712 5024 SfcI C'TryA_G Cuts at: 826 1045 2130 2516 2820 3118 4084 5219 5900 Size: 219 1085 386 304 298 966 1135 681 Cuts at: 5900 6091 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1085 966 681 386 304 298 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6736 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6736 SmaI CCC'GGG Cuts at: 1872 4063 1872 Size: 2191 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2919 4840 5741 6003 820 Size: 197 926 976 1921 901 262 1553 Fragments arranged by size: 1921 1553 976 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4527 493 Size: 4034 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6736 SphI G_CATG'C Cuts at: 1132 4094 1132 Size: 2962 3774 SspI AAT'ATT Cuts at: 5 52 4660 5 Size: 47 4608 2081 Fragments arranged by size: 4608 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6736 StyI C'CwwG_G Cuts at: 513 1065 2067 2626 2725 4175 513 Size: 552 1002 559 99 1450 3074 Fragments arranged by size: 3074 1450 1002 559 552 99 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2558 2589 2829 4763 4780 4933 5118 Size: 1255 978 31 240 1934 17 153 185 Cuts at: 5118 5563 325 Size: 445 1498 Fragments arranged by size: 1934 1498 1255 978 445 240 185 153 31 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4982 6694 96 Size: 274 80 33 51 1443 3005 1712 138 Fragments arranged by size: 3005 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2607 2639 3415 3447 3835 3867 6696 6728 2607 Size: 32 776 32 388 32 2829 32 2615 Fragments arranged by size: 2829 2615 776 388 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6736 XbaI T'CTAG_A Cuts at: 4072 4072 Size: 6736 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6736 XmaI C'CCGG_G Cuts at: 1870 4061 1870 Size: 2191 4545 XmnI GAAnn'nnTTC Cuts at: 4865 4865 Size: 6736 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4544 276 Size: 53 83 186 936 3010 2468 Fragments arranged by size: 3010 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NarI NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI