(Circular) (Six-base) MAPSORT of: pFN21AB6654.seq Check: 8800 from: 1 to: 5872 pFN21AB6654 5872 bp With 182 enzymes: SgfI * April 15, 2010 09:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3682 278 Size: 53 83 186 936 2146 2468 Fragments arranged by size: 2468 2146 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2088 3215 1937 Size: 151 1127 4594 Fragments arranged by size: 4594 1127 151 Acc65I G'GTAC_C Cuts at: 3193 3193 Size: 5872 AclI AA'CG_TT Cuts at: 1563 3999 4372 1563 Size: 2436 373 3063 Fragments arranged by size: 3063 2436 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2090 3935 5319 5587 1632 Size: 458 1845 1384 268 1917 Fragments arranged by size: 1917 1845 1384 458 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5675 AflIII A'CryG_T Cuts at: 1203 2674 4771 5748 1203 Size: 1471 2097 977 1327 Fragments arranged by size: 2097 1471 1327 977 AgeI A'CCGG_T Cuts at: 4703 4703 Size: 5872 AhdI GACnn_n'nnGTC Cuts at: 4601 4601 Size: 5872 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5872 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5872 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2895 2927 2895 Size: 32 5840 AlwNI CAG_nnn'CTG Cuts at: 2053 3615 5187 2053 Size: 1562 1572 2738 Fragments arranged by size: 2738 1572 1562 ApaLI G'TGCA_C Cuts at: 3929 5085 3929 Size: 1156 4716 ApoI r'AATT_y Cuts at: 1493 2576 3145 3181 3407 3559 5668 1493 Size: 1083 569 36 226 152 2109 1697 Fragments arranged by size: 2109 1697 1083 569 226 152 36 AseI AT'TA_AT Cuts at: 160 1032 4426 160 Size: 872 3394 1606 Fragments arranged by size: 3394 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5872 AvaI C'yCGr_G Cuts at: 1870 1943 2366 3197 1870 Size: 73 423 831 4545 Fragments arranged by size: 4545 831 423 73 BamHI G'GATC_C Cuts at: 3202 3569 3202 Size: 367 5505 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3193 4648 618 Size: 325 217 627 1406 1455 1842 Fragments arranged by size: 1842 1455 1406 627 325 217 BanII G_rGCy'C Cuts at: 1391 2079 2169 3191 1391 Size: 688 90 1022 4072 Fragments arranged by size: 4072 1022 688 90 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2528 928 Size: 971 629 4272 Fragments arranged by size: 4272 971 629 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4063 4097 1903 Size: 34 50 34 2042 34 3678 Fragments arranged by size: 3678 2042 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3764 4974 3764 Size: 1210 4662 BclI T'GATC_A Cuts at: 1548 1548 Size: 5872 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4483 136 Size: 107 122 71 631 605 2811 1525 Fragments arranged by size: 2811 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5867 5867 Size: 5872 BlpI GC'TnA_GC Cuts at: 2078 3289 2078 Size: 1211 4661 Bme1580I G_kGCm'C Cuts at: 1790 3933 5089 1790 Size: 2143 1156 2573 Fragments arranged by size: 2573 2143 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5872 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2359 2638 3017 Size: 416 53 83 399 24 942 279 379 Cuts at: 3017 3603 4561 442 Size: 586 958 1753 Fragments arranged by size: 1753 958 942 586 416 399 379 279 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5872 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4532 1239 Size: 3293 2579 Bpu10I CC'TnA_GC Cuts at: 2114 2114 Size: 5872 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2726 3997 4862 5160 2726 Size: 1271 865 298 3438 Fragments arranged by size: 3438 1271 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4535 882 Size: 617 3036 2219 Fragments arranged by size: 3036 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3663 493 Size: 711 2459 2702 Fragments arranged by size: 2702 2459 711 BsaBI GATnn'nnATC Cuts at: 5866 5866 Size: 5872 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3679 4061 4696 275 Size: 53 83 186 936 2146 382 635 1451 Fragments arranged by size: 2146 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2714 4305 4703 4977 5124 5535 2714 Size: 1591 398 274 147 411 3051 Fragments arranged by size: 3051 1591 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5647 Fragments arranged by size: 5647 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2164 2333 2384 2876 1186 Size: 978 169 51 492 4182 Fragments arranged by size: 4182 978 492 169 51 BseYI C'CCAG_C Cuts at: 5075 5571 5075 Size: 496 5376 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5299 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3322 4083 5111 1540 Size: 275 186 1321 761 1028 2301 Fragments arranged by size: 2301 1321 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3933 4018 5089 3933 Size: 85 1071 4716 Fragments arranged by size: 4716 1071 85 BsmI GAATG_Cn' Cuts at: 3383 3476 3383 Size: 93 5779 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2169 3191 3933 4018 5089 1391 Size: 399 289 90 1022 742 85 1071 2174 Fragments arranged by size: 2174 1071 1022 742 399 289 90 85 BspEI T'CCGG_A Cuts at: 5535 5535 Size: 5872 BspHI T'CATG_A Cuts at: 3761 5491 3761 Size: 1730 4142 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3227 844 Size: 2383 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2026 2118 3759 1394 Size: 429 203 92 1641 3507 Fragments arranged by size: 3507 1641 429 203 92 BsrDI GCAATG_nn' Cuts at: 65 1248 4367 4541 65 Size: 1183 3119 174 1396 Fragments arranged by size: 3119 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2107 4516 4703 1700 Size: 110 297 2409 187 2869 Fragments arranged by size: 2869 2409 297 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5872 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5872 BssSI C'ACGA_G Cuts at: 3932 4944 3932 Size: 1012 4860 BstAPI GCAn_nnn'nTGC Cuts at: 794 2053 794 Size: 1259 4613 BstBI TT'CG_AA Cuts at: 4676 4676 Size: 5872 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5872 BstYI r'GATC_y Cuts at: 1972 2154 2560 3202 3569 3954 3971 5423 5509 Size: 182 406 642 367 385 17 1452 86 Cuts at: 5509 5521 5867 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 642 406 385 367 346 182 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2169 2505 513 Size: 552 1104 336 3880 Fragments arranged by size: 3880 1104 552 336 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2430 487 Size: 683 56 1204 3929 Fragments arranged by size: 3929 1204 683 56 BtsI GCAGTG_nn' Cuts at: 2700 3388 4182 4202 2700 Size: 688 794 20 4370 Fragments arranged by size: 4370 794 688 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5872 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5837 DraI TTT'AAA Cuts at: 3176 3176 Size: 5872 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4879 809 Size: 507 3563 1802 Fragments arranged by size: 3563 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2133 3319 4208 Size: 54 1425 50 275 18 303 1186 889 Cuts at: 4208 5837 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1186 889 303 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3319 1537 Size: 275 1507 4090 Fragments arranged by size: 4090 1507 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2588 3089 3802 1353 Size: 1235 501 713 3423 Fragments arranged by size: 3423 1235 713 501 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4459 4833 4979 387 Size: 1064 138 2870 374 146 1280 Fragments arranged by size: 2870 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2090 3935 4532 5319 5587 1239 Size: 393 458 1845 597 787 268 1524 Fragments arranged by size: 1845 1524 787 597 458 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2333 2918 2333 Size: 585 5287 EcoO109I rG'GnC_Cy Cuts at: 2121 2121 Size: 5872 EcoRI G'AATT_C Cuts at: 3145 3181 3559 3145 Size: 36 378 5458 Fragments arranged by size: 5458 378 36 EcoRV GAT'ATC Cuts at: 2272 2272 Size: 5872 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3100 3100 Size: 5872 FspI TGC'GCA Cuts at: 4378 4378 Size: 5872 HaeII r_GCGC'y Cuts at: 1678 5019 1678 Size: 3341 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3118 3150 4513 4545 4587 Size: 32 135 32 1546 32 1363 32 42 Cuts at: 4587 4619 1373 Size: 32 2626 Fragments arranged by size: 2626 1546 1363 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2089 3216 3462 669 Size: 988 281 151 1127 246 3079 Fragments arranged by size: 3079 1127 988 281 246 151 HindIII A'AGCT_T Cuts at: 748 748 Size: 5872 HpaI GTT'AAC Cuts at: 3462 3462 Size: 5872 KpnI G_GTAC'C Cuts at: 3197 3197 Size: 5872 MfeI C'AATT_G Cuts at: 3471 3471 Size: 5872 MluI A'CGCG_T Cuts at: 5748 5748 Size: 5872 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3161 4986 5170 1305 Size: 1856 1825 184 2007 Fragments arranged by size: 2007 1856 1825 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2135 10 Size: 54 1425 343 303 3747 Fragments arranged by size: 3747 1425 343 303 54 MslI CAynn'nnrTG Cuts at: 518 1166 2945 2960 3830 4189 4348 518 Size: 648 1779 15 870 359 159 2042 Fragments arranged by size: 2042 1779 870 648 359 159 15 MspA1I CmG'CkG Cuts at: 1606 2053 2068 2192 2218 2481 3288 3965 5113 Size: 447 15 124 26 263 807 677 1148 Cuts at: 5113 5358 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 807 677 447 263 245 124 26 15 NaeI GCC'GGC Cuts at: 2109 2109 Size: 5872 NcoI C'CATG_G Cuts at: 513 1065 2505 513 Size: 552 1440 3880 Fragments arranged by size: 3880 1440 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5872 NgoMIV G'CCGG_C Cuts at: 2107 2107 Size: 5872 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5872 NotI GC'GGCC_GC Cuts at: 3319 3319 Size: 5872 NspI r_CATG'y Cuts at: 1132 1229 3230 4775 1132 Size: 97 2001 1545 2229 Fragments arranged by size: 2229 2001 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5872 PciI A'CATG_T Cuts at: 4771 4771 Size: 5872 PflMI CCAn_nnn'nTGG Cuts at: 1108 2395 1108 Size: 1287 4585 PfoI T'CCnGG_A Cuts at: 2160 2160 Size: 5872 PmeI GTTT'AAAC Cuts at: 3176 3176 Size: 5872 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3941 3973 5454 5486 3941 Size: 32 1481 32 4327 Fragments arranged by size: 4327 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2121 2121 Size: 5872 PshAI GACnn'nnGTC Cuts at: 1613 2126 1613 Size: 513 5359 PsiI TTA'TAA Cuts at: 3442 3442 Size: 5872 PstI C_TGCA'G Cuts at: 830 2058 2695 3224 830 Size: 1228 637 529 3478 Fragments arranged by size: 3478 1228 637 529 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5872 PvuII CAG'CTG Cuts at: 2053 2192 2053 Size: 139 5733 SalI G'TCGA_C Cuts at: 1936 2087 3214 1936 Size: 151 1127 4594 Fragments arranged by size: 4594 1127 151 SanDI GG'GwC_CC Cuts at: 2121 2121 Size: 5872 SbfI CC_TGCA'GG Cuts at: 3224 3224 Size: 5872 ScaI AGT'ACT Cuts at: 4120 5832 4120 Size: 1712 4160 SexAI A'CCwGG_T Cuts at: 2841 2841 Size: 5872 SfcI C'TryA_G Cuts at: 826 1045 2054 2691 3220 4355 5036 5227 826 Size: 219 1009 637 529 1135 681 191 1471 Fragments arranged by size: 1471 1135 1009 681 637 529 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5872 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5872 SmaI CCC'GGG Cuts at: 1872 3199 1872 Size: 1327 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2741 3976 4877 5139 820 Size: 197 926 798 1235 901 262 1553 Fragments arranged by size: 1553 1235 926 901 798 262 197 SnaBI TAC'GTA Cuts at: 493 3663 493 Size: 3170 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5872 SphI G_CATG'C Cuts at: 1132 3230 1132 Size: 2098 3774 SspI AAT'ATT Cuts at: 5 52 3061 3796 5 Size: 47 3009 735 2081 Fragments arranged by size: 3009 2081 735 47 StuI AGG'CCT Cuts at: 1517 2924 1517 Size: 1407 4465 StyI C'CwwG_G Cuts at: 513 1065 2334 2505 3156 3311 513 Size: 552 1269 171 651 155 3074 Fragments arranged by size: 3074 1269 651 552 171 155 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3899 3916 4069 4254 4699 325 Size: 1255 2319 17 153 185 445 1498 Fragments arranged by size: 2319 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4118 5830 96 Size: 274 80 33 51 1443 2141 1712 138 Fragments arranged by size: 2141 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2339 2371 5832 5864 2339 Size: 32 3461 32 2347 Fragments arranged by size: 3461 2347 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5872 XbaI T'CTAG_A Cuts at: 3208 3208 Size: 5872 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5872 XmaI C'CCGG_G Cuts at: 1870 3197 1870 Size: 1327 4545 XmnI GAAnn'nnTTC Cuts at: 4001 4001 Size: 5872 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3680 276 Size: 53 83 186 936 2146 2468 Fragments arranged by size: 2468 2146 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI XcmI