Restriction map for
pFN21AE1032
##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspEI AspI AvaI AviII
BamHI BanI BbeI BbrPI BbvCI
BcgI BfrI BglI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsoBI BspDI BspEI
BspHI BsrBI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV FseI FspI HaeII HhaI
HinP1I HpaI KasI KpnI KspI
MamI MluI MroI MvnI NarI
NcoI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI Sse8387I Tth111I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AcyI 2061 GACGCC G[GA]^CG[CT]C
AvrII 894 CCTAGG C^CTAGG
BalI 483 TGGCCA TGG^CCA
BanII 329 GAGCCC G[GA]GC[CT]^C
BciVI 2942 GTATCC GTATCC(6/5)
BclI 1506 TGATCA T^GATCA
BglII 3102 AGATCT A^GATCT
BlnI 894 CCTAGG C^CTAGG
BsaHI 2061 GACGCC G[GA]^CG[CT]C
BsmBI 2211 CGTCTC CGTCTC(1/5)
BsmI 3270 GAATGC GAATGC(1/-1)
BspLU11I 917 ACATGT A^CATGT
BsrFI 3407 GCCGGC [GA]^CCGG[CT]
BssSI 746 CACGAG CACGAG(-5/-1)
Cfr10I 3407 GCCGGC [GA]^CCGG[CT]
DraII 3863 AGGCCCC [GA]G^G[ACGT]CC[CT]
EaeI 483 TGGCCA [CT]^GGCC[GA]
EcoO109I 3863 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 2198 ATGCAT ATGCA^T
FauI 1150 CCCGC CCCGC(4/6)
FbaI 1506 TGATCA T^GATCA
Hin1I 2061 GACGCC G[GA]^CG[CT]C
MfeI 881 CAATTG C^AATTG
MluNI 483 TGGCCA TGG^CCA
MscI 483 TGGCCA TGG^CCA
MunI 881 CAATTG C^AATTG
NaeI 3407 GCCGGC GCC^GGC
NdeI 2908 CATATG CA^TATG
NgoMIV 3407 GCCGGC G^CCGGC
NheI 786 GCTAGC G^CTAGC
NsiI 2198 ATGCAT ATGCA^T
SphI 293 GCATGC GCATG^C
SspI 826 AATATT AAT^ATT
StuI 1277 AGGCCT AGG^CCT
SwaI 1841 ATTTAAAT ATTT^AAAT
XcmI 1043 CCAGTCAACCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccI 626 GTAGAC GT^[AC][GT]AC
4246 GTAGAC
AflIII 917 ACATGT A^C[GA][CT]GT
3183 ACGTGT
AspHI 584 GTGCTC G[AT]GC[AT]^C
4092 GAGCAC
AvaII 267 GGACC G^G[AT]CC
3915 GGTCC
BsgI 2681 CTGCAC GTGCAG(16/14)
401 GTGCAG
BsiHKAI 584 GTGCTC G[AT]GC[AT]^C
4092 GAGCAC
Bsp1407I 121 TGTACA T^GTACA
1159 TGTACA
BsrDI 2496 CATTGC GCAATG(2/0)
4265 CATTGC
BsrGI 121 TGTACA T^GTACA
1159 TGTACA
DraIII 1394 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
2519 CACCATGTG
HgaI 629 GACGC GACGC(5/10)
2061 GACGC
PflMI 2826 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3240 CCATAAGATGG
PstI 2465 CTGCAG CTGCA^G
3945 CTGCAG
SpeI 814 ACTAGT A^CTAGT
2355 ACTAGT
SspBI 121 TGTACA T^GTACA
1159 TGTACA
Van91I 2826 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3240 CCATAAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI EarI
EcoNI EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PshBI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII