(Circular) (Six-base) MAPSORT of: pFN21AE1032.seq Check: 1903 from: 1 to: 8986 pFN21AE1032 8986 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6796 278 Size: 53 83 186 936 5260 2468 Fragments arranged by size: 5260 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2632 6252 6329 1937 Size: 695 3620 77 4594 Fragments arranged by size: 4594 3620 695 77 Acc65I G'GTAC_C Cuts at: 6307 6307 Size: 8986 AclI AA'CG_TT Cuts at: 1563 7113 7486 1563 Size: 5550 373 3063 Fragments arranged by size: 5550 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2949 4113 4362 4482 5100 6030 7049 8433 Size: 1317 1164 249 120 618 930 1019 1384 Cuts at: 8433 8701 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1317 1164 1019 930 618 268 249 120 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8789 AflIII A'CryG_T Cuts at: 1203 2922 5188 7885 8862 1203 Size: 1719 2266 2697 977 1327 Fragments arranged by size: 2697 2266 1719 1327 977 AgeI A'CCGG_T Cuts at: 7817 7817 Size: 8986 AhdI GACnn_n'nnGTC Cuts at: 7715 7715 Size: 8986 AleI CACnn'nnGTG Cuts at: 1166 5187 1166 Size: 4021 4965 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4951 1184 Size: 3767 5219 AlwNI CAG_nnn'CTG Cuts at: 3926 4118 4265 4859 4931 6729 8301 3926 Size: 192 147 594 72 1798 1572 4611 Fragments arranged by size: 4611 1798 1572 594 192 147 72 ApaLI G'TGCA_C Cuts at: 7043 8199 7043 Size: 1156 7830 ApoI r'AATT_y Cuts at: 1493 2493 2695 2810 2894 2916 3247 3376 3661 Size: 1000 202 115 84 22 331 129 285 Cuts at: 3661 3726 3870 3976 4681 5551 6175 6257 6295 Size: 65 144 106 705 870 624 82 38 Cuts at: 6295 6521 6673 8782 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1000 870 705 624 331 285 226 202 152 144 129 115 106 84 82 65 38 22 AseI AT'TA_AT Cuts at: 160 1032 3690 3750 4293 6008 7540 160 Size: 872 2658 60 543 1715 1532 1606 Fragments arranged by size: 2658 1715 1606 1532 872 543 60 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8986 AvaI C'yCGr_G Cuts at: 1870 1943 6311 1870 Size: 73 4368 4545 Fragments arranged by size: 4545 4368 73 AvrII C'CTAG_G Cuts at: 2899 2899 Size: 8986 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2457 2490 3282 3315 5667 5700 2457 Size: 33 792 33 2352 33 5743 Fragments arranged by size: 5743 2352 792 33 33 33 BamHI G'GATC_C Cuts at: 6316 6683 6316 Size: 367 8619 BanI G'GyrC_C Cuts at: 618 943 1160 1787 6307 7762 618 Size: 325 217 627 4520 1455 1842 Fragments arranged by size: 4520 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2338 6305 1391 Size: 947 3967 4072 Fragments arranged by size: 4072 3967 947 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3243 3468 3797 3885 4317 4470 5901 Size: 971 1344 225 329 88 432 153 1431 Cuts at: 5901 928 Size: 4013 Fragments arranged by size: 4013 1431 1344 971 432 329 225 153 88 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7177 7211 1903 Size: 34 5240 34 3678 Fragments arranged by size: 5240 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4958 6878 8088 4958 Size: 1920 1210 5856 Fragments arranged by size: 5856 1920 1210 BclI T'GATC_A Cuts at: 1548 3511 1548 Size: 1963 7023 BfrBI ATG'CAT Cuts at: 4205 4205 Size: 8986 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7597 136 Size: 107 122 71 631 605 5925 1525 Fragments arranged by size: 5925 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5107 8981 5107 Size: 3874 5112 BlpI GC'TnA_GC Cuts at: 6403 6403 Size: 8986 Bme1580I G_kGCm'C Cuts at: 1790 5808 7047 8203 1790 Size: 4018 1239 1156 2573 Fragments arranged by size: 4018 2573 1239 1156 BmgBI CAC'GTC Cuts at: 1318 5189 1318 Size: 3871 5115 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3066 4847 6219 Size: 416 53 83 399 24 1649 1781 1372 Cuts at: 6219 6717 7675 442 Size: 498 958 1753 Fragments arranged by size: 1781 1753 1649 1372 958 498 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2795 1056 Size: 1739 7247 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3513 6163 3513 Size: 2650 6336 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2382 2583 4056 4095 4797 6004 7646 1239 Size: 1143 201 1473 39 702 1207 1642 2579 Fragments arranged by size: 2579 1642 1473 1207 1143 702 201 39 Bpu10I CC'TnA_GC Cuts at: 2592 2847 3957 4986 5821 2592 Size: 255 1110 1029 835 5757 Fragments arranged by size: 5757 1110 1029 835 255 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 7111 7976 8274 7111 Size: 865 298 7823 Fragments arranged by size: 7823 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7649 882 Size: 617 6150 2219 Fragments arranged by size: 6150 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 6777 493 Size: 711 5573 2702 Fragments arranged by size: 5573 2702 711 BsaBI GATnn'nnATC Cuts at: 8980 8980 Size: 8986 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4067 6793 7175 7810 Size: 53 83 186 936 2534 2726 382 635 Cuts at: 7810 275 Size: 1451 Fragments arranged by size: 2726 2534 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7419 7817 8091 8238 8649 7419 Size: 398 274 147 411 7756 Fragments arranged by size: 7756 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2437 2467 5200 5230 1178 Size: 30 165 30 1034 30 2733 30 4934 Fragments arranged by size: 4934 2733 1034 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2442 4884 4887 5361 6045 1186 Size: 1256 2442 3 474 684 4127 Fragments arranged by size: 4127 2442 1256 684 474 3 BseYI C'CCAG_C Cuts at: 2239 2971 8189 8685 2239 Size: 732 5218 496 2540 Fragments arranged by size: 5218 2540 732 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2427 4671 1163 Size: 573 691 2244 5478 Fragments arranged by size: 5478 2244 691 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6436 7197 8225 1540 Size: 275 186 4435 761 1028 2301 Fragments arranged by size: 4435 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2593 6101 7047 7132 8203 2593 Size: 3508 946 85 1071 3376 Fragments arranged by size: 3508 3376 1071 946 85 BsmI GAATG_Cn' Cuts at: 5281 6497 6590 5281 Size: 1216 93 7677 Fragments arranged by size: 7677 1216 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4222 4222 Size: 8986 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2338 2593 5808 6101 6305 7047 7132 Size: 399 548 255 3215 293 204 742 85 Cuts at: 7132 8203 1391 Size: 1071 2174 Fragments arranged by size: 3215 2174 1071 742 548 399 293 255 204 85 BspEI T'CCGG_A Cuts at: 8649 8649 Size: 8986 BspHI T'CATG_A Cuts at: 6875 8605 6875 Size: 1730 7256 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2081 4314 4894 6341 844 Size: 1237 2233 580 1447 3489 Fragments arranged by size: 3489 2233 1447 1237 580 BsrBI CCG'CTC Cuts at: 1394 1823 6873 1394 Size: 429 5050 3507 Fragments arranged by size: 5050 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4500 6269 7481 7655 65 Size: 1183 3252 1769 1212 174 1396 Fragments arranged by size: 3252 1769 1396 1212 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5412 7630 7817 1700 Size: 110 3602 2218 187 2869 Fragments arranged by size: 3602 2869 2218 187 110 BsrGI T'GTAC_A Cuts at: 96 2126 3164 96 Size: 2030 1038 5918 Fragments arranged by size: 5918 2030 1038 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8986 BssSI C'ACGA_G Cuts at: 2751 7046 8058 2751 Size: 4295 1012 3679 Fragments arranged by size: 4295 3679 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8986 BstBI TT'CG_AA Cuts at: 7790 7790 Size: 8986 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8986 BstYI r'GATC_y Cuts at: 1972 2500 2704 4790 5107 5689 6316 6683 7068 Size: 528 204 2086 317 582 627 367 385 Cuts at: 7068 7085 8537 8623 8635 8981 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 2086 1977 1452 627 582 528 385 367 346 317 204 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 8434 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5538 487 Size: 683 56 4312 3935 Fragments arranged by size: 4312 3935 683 56 BtsI GCAGTG_nn' Cuts at: 5612 6502 7296 7316 5612 Size: 890 794 20 7282 Fragments arranged by size: 7282 890 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8986 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5092 5127 529 Size: 35 4528 35 4388 Fragments arranged by size: 4528 4388 35 35 DraI TTT'AAA Cuts at: 2106 2193 2574 3289 3849 6290 2106 Size: 87 381 715 560 2441 4802 Fragments arranged by size: 4802 2441 715 560 381 87 DraIII CAC_nnn'GTG Cuts at: 3404 4529 3404 Size: 1125 7861 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7993 809 Size: 507 6677 1802 Fragments arranged by size: 6677 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2488 6433 7322 Size: 54 1425 50 275 18 658 3945 889 Cuts at: 7322 8951 8 Size: 1629 43 Fragments arranged by size: 3945 1629 1425 889 658 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6433 1537 Size: 275 4621 4090 Fragments arranged by size: 4621 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2784 3584 4002 4110 5145 6916 1353 Size: 1431 800 418 108 1035 1771 3423 Fragments arranged by size: 3423 1771 1431 1035 800 418 108 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5704 7573 7947 8093 387 Size: 1064 138 4115 1869 374 146 1280 Fragments arranged by size: 4115 1869 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2382 2583 2949 4056 4095 4113 4362 Size: 393 750 201 366 1107 39 18 249 Cuts at: 4362 4482 4797 5100 6004 6030 7049 7646 8433 Size: 120 315 303 904 26 1019 597 787 Cuts at: 8433 8701 1239 Size: 268 1524 Fragments arranged by size: 1524 1107 1019 904 787 750 597 393 366 315 303 268 249 201 120 39 26 18 EcoNI CCTnn'n_nnAGG Cuts at: 2460 4074 5010 5820 5878 2460 Size: 1614 936 810 58 5568 Fragments arranged by size: 5568 1614 936 810 58 EcoO109I rG'GnC_Cy Cuts at: 5869 5869 Size: 8986 EcoRI G'AATT_C Cuts at: 2894 3376 3976 6295 6673 2894 Size: 482 600 2319 378 5207 Fragments arranged by size: 5207 2319 600 482 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2682 4596 2682 Size: 1914 7072 FspI TGC'GCA Cuts at: 7492 7492 Size: 8986 HaeII r_GCGC'y Cuts at: 1678 8133 1678 Size: 6455 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3128 3160 3875 3907 3908 Size: 32 135 32 1556 32 715 32 1 Cuts at: 3908 3940 3977 4009 4412 4444 5178 5210 7627 Size: 32 37 32 403 32 734 32 2417 Cuts at: 7627 7659 7701 7733 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2417 1556 734 715 403 135 42 37 32 32 32 32 32 32 32 32 32 32 1 HincII GTy'rAC Cuts at: 669 1657 1938 2530 3053 4759 6330 6576 669 Size: 988 281 592 523 1706 1571 246 3079 Fragments arranged by size: 3079 1706 1571 988 592 523 281 246 HindIII A'AGCT_T Cuts at: 748 2378 2568 6218 748 Size: 1630 190 3650 3516 Fragments arranged by size: 3650 3516 1630 190 HpaI GTT'AAC Cuts at: 6576 6576 Size: 8986 KpnI G_GTAC'C Cuts at: 6311 6311 Size: 8986 MfeI C'AATT_G Cuts at: 2886 6585 2886 Size: 3699 5287 MluI A'CGCG_T Cuts at: 8862 8862 Size: 8986 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4652 4846 5038 8100 8284 1305 Size: 3347 194 192 3062 184 2007 Fragments arranged by size: 3347 3062 2007 194 192 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2490 10 Size: 54 1425 343 658 6506 Fragments arranged by size: 6506 1425 658 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2257 3849 4933 5187 5738 6008 6279 Size: 648 1091 1592 1084 254 551 270 271 Cuts at: 6279 6944 7303 7462 518 Size: 665 359 159 2042 Fragments arranged by size: 2042 1592 1091 1084 665 648 551 359 271 270 254 159 MspA1I CmG'CkG Cuts at: 1606 3296 5168 5681 6402 7079 8227 8472 1606 Size: 1690 1872 513 721 677 1148 245 2120 Fragments arranged by size: 2120 1872 1690 1148 721 677 513 245 NaeI GCC'GGC Cuts at: 5414 5414 Size: 8986 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 8434 NdeI CA'TA_TG Cuts at: 387 4914 387 Size: 4527 4459 NgoMIV G'CCGG_C Cuts at: 5412 5412 Size: 8986 NheI G'CTAG_C Cuts at: 1052 2791 1052 Size: 1739 7247 NotI GC'GGCC_GC Cuts at: 6433 6433 Size: 8986 NsiI A_TGCA'T Cuts at: 4207 4207 Size: 8986 NspI r_CATG'y Cuts at: 1132 1229 2302 2612 2926 6344 7889 1132 Size: 97 1073 310 314 3418 1545 2229 Fragments arranged by size: 3418 2229 1545 1073 314 310 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8986 PciI A'CATG_T Cuts at: 2922 7885 2922 Size: 4963 4023 PflMI CCAn_nnn'nTGG Cuts at: 1108 4837 5251 1108 Size: 3729 414 4843 Fragments arranged by size: 4843 3729 414 PmeI GTTT'AAAC Cuts at: 6290 6290 Size: 8986 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7055 7087 8568 8600 7055 Size: 32 1481 32 7441 Fragments arranged by size: 7441 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8986 PsiI TTA'TAA Cuts at: 6556 6556 Size: 8986 PstI C_TGCA'G Cuts at: 830 4474 5954 6338 830 Size: 3644 1480 384 3478 Fragments arranged by size: 3644 3478 1480 384 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8986 PvuII CAG'CTG Cuts at: 3296 5168 5681 3296 Size: 1872 513 6601 Fragments arranged by size: 6601 1872 513 SalI G'TCGA_C Cuts at: 1936 6328 1936 Size: 4392 4594 SbfI CC_TGCA'GG Cuts at: 6338 6338 Size: 8986 ScaI AGT'ACT Cuts at: 7234 8946 7234 Size: 1712 7274 SfcI C'TryA_G Cuts at: 826 1045 3450 4086 4389 4470 5604 5853 5950 Size: 219 2405 636 303 81 1134 249 97 Cuts at: 5950 6334 7469 8150 8341 826 Size: 384 1135 681 191 1471 Fragments arranged by size: 2405 1471 1135 1134 681 636 384 303 249 219 191 97 81 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8986 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8986 SmaI CCC'GGG Cuts at: 1872 6313 1872 Size: 4441 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 7090 7991 8253 820 Size: 197 926 5147 901 262 1553 Fragments arranged by size: 5147 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6777 493 Size: 6284 2702 SpeI A'CTAG_T Cuts at: 152 2819 4360 152 Size: 2667 1541 4778 Fragments arranged by size: 4778 2667 1541 SphI G_CATG'C Cuts at: 1132 2302 6344 1132 Size: 1170 4042 3774 Fragments arranged by size: 4042 3774 1170 SspI AAT'ATT Cuts at: 5 52 2833 6910 5 Size: 47 2781 4077 2081 Fragments arranged by size: 4077 2781 2081 47 StuI AGG'CCT Cuts at: 1517 3284 1517 Size: 1767 7219 StyI C'CwwG_G Cuts at: 513 1065 2312 2899 4536 4733 4831 6425 513 Size: 552 1247 587 1637 197 98 1594 3074 Fragments arranged by size: 3074 1637 1594 1247 587 552 197 98 SwaI ATTT'AAAT Cuts at: 3849 3849 Size: 8986 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5320 7013 7030 7183 7368 7813 325 Size: 1255 3740 1693 17 153 185 445 1498 Fragments arranged by size: 3740 1693 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2126 3164 4825 Size: 274 80 33 51 1443 149 1038 1661 Cuts at: 4825 5191 5662 5903 7232 8944 96 Size: 366 471 241 1329 1712 138 Fragments arranged by size: 1712 1661 1443 1329 1038 471 366 274 241 149 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4136 4168 5200 5232 8946 8978 4136 Size: 32 1032 32 3714 32 4144 Fragments arranged by size: 4144 3714 1032 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8986 XbaI T'CTAG_A Cuts at: 6322 6322 Size: 8986 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3055 3055 Size: 8986 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8986 XmaI C'CCGG_G Cuts at: 1870 6311 1870 Size: 4441 4545 XmnI GAAnn'nnTTC Cuts at: 7115 7115 Size: 8986 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6794 276 Size: 53 83 186 936 5260 2468 Fragments arranged by size: 5260 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BbeI BbvCI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NarI NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI