Restriction map for
pFN21AE1032
(Linear) (Six-base) MAPSORT of: KIEE1032.seq Check: 1513 from: 1 to: 4281
KIEE1032.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 0 627 4247 4281
Size: 627 3620 34
Fragments arranged by size:
3620 627 34
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 944 2108 2357 2477 3095 4025 4281
Size: 944 1164 249 120 618 930 256
Fragments arranged by size:
1164 944 930 618 256 249 120
AflIII A'CryG_T
Cuts at: 0 917 3183 4281
Size: 917 2266 1098
Fragments arranged by size:
2266 1098 917
AleI CACnn'nnGTG
Cuts at: 0 3182 4281
Size: 3182 1099
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2946 4281
Size: 2946 1335
AlwNI CAG_nnn'CTG
Cuts at: 0 1921 2113 2260 2854 2926 4281
Size: 1921 192 147 594 72 1355
Fragments arranged by size:
1921 1355 594 192 147 72
ApoI r'AATT_y
Cuts at: 0 488 690 805 889 911 1242 1371 1656
Size: 488 202 115 84 22 331 129 285
Cuts at: 1656 1721 1865 1971 2676 3546 4170 4252 4281
Size: 65 144 106 705 870 624 82 29
Fragments arranged by size:
870 705 624 488 331 285 202 144
129 115 106 84 82 65 29 22
AseI AT'TA_AT
Cuts at: 0 1685 1745 2288 4003 4281
Size: 1685 60 543 1715 278
Fragments arranged by size:
1715 1685 543 278 60
AvrII C'CTAG_G
Cuts at: 0 894 4281
Size: 894 3387
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 452 485 1277 1310 3662 3695 4281
Size: 452 33 792 33 2352 33 586
Fragments arranged by size:
2352 792 586 452 33 33 33
BanII G_rGCy'C
Cuts at: 0 333 4281
Size: 333 3948
BbsI GAAGACnn'nnnn_
Cuts at: 0 1238 1463 1792 1880 2312 2465 3896 4281
Size: 1238 225 329 88 432 153 1431 385
Fragments arranged by size:
1431 1238 432 385 329 225 153 88
BciVI GTATCCnnnnn_n'
Cuts at: 0 2953 4281
Size: 2953 1328
BclI T'GATC_A
Cuts at: 0 1506 4281
Size: 1506 2775
BfrBI ATG'CAT
Cuts at: 0 2200 4281
Size: 2200 2081
BglII A'GATC_T
Cuts at: 0 3102 4281
Size: 3102 1179
Bme1580I G_kGCm'C
Cuts at: 0 3803 4281
Size: 3803 478
BmgBI CAC'GTC
Cuts at: 0 3184 4281
Size: 3184 1097
BmrI ACTGGGnnnn_n'
Cuts at: 0 1061 2842 4214 4281
Size: 1061 1781 1372 67
Fragments arranged by size:
1781 1372 1061 67
BmtI G_CTAG'C
Cuts at: 0 790 4281
Size: 790 3491
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1508 4158 4281
Size: 1508 2650 123
Fragments arranged by size:
2650 1508 123
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 578 2051 2090 2792 3999 4281
Size: 377 201 1473 39 702 1207 282
Fragments arranged by size:
1473 1207 702 377 282 201 39
Bpu10I CC'TnA_GC
Cuts at: 0 587 842 1952 2981 3816 4281
Size: 587 255 1110 1029 835 465
Fragments arranged by size:
1110 1029 835 587 465 255
BsaHI Gr'CG_yC
Cuts at: 0 2062 4281
Size: 2062 2219
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 432 462 3195 3225 4281
Size: 432 30 2733 30 1056
Fragments arranged by size:
2733 1056 432 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 437 2879 2882 3356 4040 4281
Size: 437 2442 3 474 684 241
Fragments arranged by size:
2442 684 474 437 241 3
BseYI C'CCAG_C
Cuts at: 0 234 966 4281
Size: 234 732 3315
Fragments arranged by size:
3315 732 234
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 422 2666 4281
Size: 422 2244 1615
Fragments arranged by size:
2244 1615 422
BsiHKAI G_wGCw'C
Cuts at: 0 588 4096 4281
Size: 588 3508 185
Fragments arranged by size:
3508 588 185
BsmI GAATG_Cn'
Cuts at: 0 3276 4281
Size: 3276 1005
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2217 4281
Size: 2217 2064
Bsp1286I G_dGCh'C
Cuts at: 0 333 588 3803 4096 4281
Size: 333 255 3215 293 185
Fragments arranged by size:
3215 333 293 255 185
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 76 2309 2889 4281
Size: 76 2233 580 1392
Fragments arranged by size:
2233 1392 580 76
BsrDI GCAATG_nn'
Cuts at: 0 2495 4264 4281
Size: 2495 1769 17
Fragments arranged by size:
2495 1769 17
BsrFI r'CCGG_y
Cuts at: 0 3407 4281
Size: 3407 874
BsrGI T'GTAC_A
Cuts at: 0 121 1159 4281
Size: 121 1038 3122
Fragments arranged by size:
3122 1038 121
BssSI C'ACGA_G
Cuts at: 0 746 4281
Size: 746 3535
BstYI r'GATC_y
Cuts at: 0 495 699 2785 3102 3684 4281
Size: 495 204 2086 317 582 597
Fragments arranged by size:
2086 597 582 495 317 204
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3533 4281
Size: 3533 748
BtsI GCAGTG_nn'
Cuts at: 0 3607 4281
Size: 3607 674
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3087 3122 4281
Size: 3087 35 1159
Fragments arranged by size:
3087 1159 35
DraI TTT'AAA
Cuts at: 0 101 188 569 1284 1844 4281
Size: 101 87 381 715 560 2437
Fragments arranged by size:
2437 715 560 381 101 87
DraIII CAC_nnn'GTG
Cuts at: 0 1399 2524 4281
Size: 1399 1125 1757
Fragments arranged by size:
1757 1399 1125
EaeI y'GGCC_r
Cuts at: 0 483 4281
Size: 483 3798
EarI CTCTTCn'nnn_
Cuts at: 0 779 1579 1997 2105 3140 4281
Size: 779 800 418 108 1035 1141
Fragments arranged by size:
1141 1035 800 779 418 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3699 4281
Size: 3699 582
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 578 944 2051 2090 2108 2357 2477
Size: 377 201 366 1107 39 18 249 120
Cuts at: 2477 2792 3095 3999 4025 4281
Size: 315 303 904 26 256
Fragments arranged by size:
1107 904 377 366 315 303 256 249
201 120 39 26 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 455 2069 3005 3815 3873 4281
Size: 455 1614 936 810 58 408
Fragments arranged by size:
1614 936 810 455 408 58
EcoO109I rG'GnC_Cy
Cuts at: 0 3864 4281
Size: 3864 417
EcoRI G'AATT_C
Cuts at: 0 889 1371 1971 4281
Size: 889 482 600 2310
Fragments arranged by size:
2310 889 600 482
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 677 2591 4281
Size: 677 1914 1690
Fragments arranged by size:
1914 1690 677
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1123 1155 1870 1902 1903 1935 1972 2004
Size: 1123 32 715 32 1 32 37 32
Cuts at: 2004 2407 2439 3173 3205 4281
Size: 403 32 734 32 1076
Fragments arranged by size:
1123 1076 734 715 403 37 32 32
32 32 32 32 1
HincII GTy'rAC
Cuts at: 0 525 1048 2754 4281
Size: 525 523 1706 1527
Fragments arranged by size:
1706 1527 525 523
HindIII A'AGCT_T
Cuts at: 0 373 563 4213 4281
Size: 373 190 3650 68
Fragments arranged by size:
3650 373 190 68
MfeI C'AATT_G
Cuts at: 0 881 4281
Size: 881 3400
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2647 2841 3033 4281
Size: 2647 194 192 1248
Fragments arranged by size:
2647 1248 194 192
MscI TGG'CCA
Cuts at: 0 485 4281
Size: 485 3796
MslI CAynn'nnrTG
Cuts at: 0 252 1844 2928 3182 3733 4003 4274 4281
Size: 252 1592 1084 254 551 270 271 7
Fragments arranged by size:
1592 1084 551 271 270 254 252 7
MspA1I CmG'CkG
Cuts at: 0 1291 3163 3676 4281
Size: 1291 1872 513 605
Fragments arranged by size:
1872 1291 605 513
NaeI GCC'GGC
Cuts at: 0 3409 4281
Size: 3409 872
NdeI CA'TA_TG
Cuts at: 0 2909 4281
Size: 2909 1372
NgoMIV G'CCGG_C
Cuts at: 0 3407 4281
Size: 3407 874
NheI G'CTAG_C
Cuts at: 0 786 4281
Size: 786 3495
NsiI A_TGCA'T
Cuts at: 0 2202 4281
Size: 2202 2079
NspI r_CATG'y
Cuts at: 0 297 607 921 4281
Size: 297 310 314 3360
Fragments arranged by size:
3360 314 310 297
PciI A'CATG_T
Cuts at: 0 917 4281
Size: 917 3364
PflMI CCAn_nnn'nTGG
Cuts at: 0 2832 3246 4281
Size: 2832 414 1035
Fragments arranged by size:
2832 1035 414
PstI C_TGCA'G
Cuts at: 0 2469 3949 4281
Size: 2469 1480 332
Fragments arranged by size:
2469 1480 332
PvuII CAG'CTG
Cuts at: 0 1291 3163 3676 4281
Size: 1291 1872 513 605
Fragments arranged by size:
1872 1291 605 513
SfcI C'TryA_G
Cuts at: 0 1445 2081 2384 2465 3599 3848 3945 4281
Size: 1445 636 303 81 1134 249 97 336
Fragments arranged by size:
1445 1134 636 336 303 249 97 81
SpeI A'CTAG_T
Cuts at: 0 814 2355 4281
Size: 814 1541 1926
Fragments arranged by size:
1926 1541 814
SphI G_CATG'C
Cuts at: 0 297 4281
Size: 297 3984
SspI AAT'ATT
Cuts at: 0 828 4281
Size: 828 3453
StuI AGG'CCT
Cuts at: 0 1279 4281
Size: 1279 3002
StyI C'CwwG_G
Cuts at: 0 307 894 2531 2728 2826 4281
Size: 307 587 1637 197 98 1455
Fragments arranged by size:
1637 1455 587 307 197 98
SwaI ATTT'AAAT
Cuts at: 0 1844 4281
Size: 1844 2437
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3315 4281
Size: 3315 966
TatI w'GTAC_w
Cuts at: 0 121 1159 2820 3186 3657 3898 4281
Size: 121 1038 1661 366 471 241 383
Fragments arranged by size:
1661 1038 471 383 366 241 121
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2131 2163 3195 3227 4281
Size: 2131 32 1032 32 1054
Fragments arranged by size:
2131 1054 1032 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1050 4281
Size: 1050 3231
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI AlwNI ApoI AseI
AvrII BaeI BanII BbsI BciVI BclI BfrBI BglII
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrDI BsrFI BsrGI BssSI BstYI BtgZI BtsI
CspCI DraI DraIII EaeI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI Hin4I HincII HindIII MfeI MmeI
MscI MslI MspA1I NaeI NdeI NgoMIV NheI NsiI
NspI PciI PflMI PstI PvuII SfcI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaI ApaLI AscI AsiSI AvaI BamHI BanI
BbeI BbvCI BcgI BglI BlpI BpuEI BsaI BsaAI
BsaBI BsaWI BsiEI BsiWI BspEI BspHI BsrBI BssHII
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI ClaI
DrdI EagI EcoICRI EcoRV FseI FspI FspAI HaeII
HpaI KasI KpnI MluI NarI NcoI NotI NruI
PacI PasI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SrfI Tth111I XbaI
XhoI XmaI XmnI ZraI