Restriction map for
pFN21AE1031
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# NO CUT ENZYMES ( 73 enzymes) #
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AccI AclI AcsI AgeI Alw44I
ApaLI ApoI AscI AseI AsnI
AviII BamHI BcgI BciVI BsaBI
BsiEI BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraI DraIII
EagI EclXI Eco52I EcoRI EcoRV
EcoT22I FseI FspI HincII HindII
HpaI MamI MfeI MluI MunI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI SspBI
SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 3612 GACGTC GACGT^C
Acc65I 1454 GGTACC G^GTACC
AflII 1302 CTTAAG C^TTAAG
AflIII 3753 ACACGT A^C[GA][CT]GT
Aor51HI 2898 AGCGCT AGC^GCT
Asp700I 2775 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1454 GGTACC G^GTACC
AspI 988 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 568 CCTAGG C^CTAGG
BbeI 3425 GGCGCC GGCGC^C
BbrPI 3754 CACGTG CAC^GTG
BclI 294 TGATCA T^GATCA
BfrI 1302 CTTAAG C^TTAAG
BglII 787 AGATCT A^GATCT
BlnI 568 CCTAGG C^CTAGG
BsmBI 2641 CGTCTC CGTCTC(1/5)
BssSI 2608 CTCGTG CACGAG(-5/-1)
BstEII 2062 GGTTACC G^GT[ACGT]ACC
BstPI 2062 GGTTACC G^GT[ACGT]ACC
DrdI 2283 GACACAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2898 AGCGCT AGC^GCT
EcoO65I 2062 GGTTACC G^GT[ACGT]ACC
FbaI 294 TGATCA T^GATCA
HindIII 4060 AAGCTT A^AGCTT
KasI 3425 GGCGCC G^GCGCC
KpnI 1454 GGTACC GGTAC^C
KspI 2116 CCGCGG CCGC^GG
NarI 3425 GGCGCC GG^CGCC
NheI 1287 GCTAGC G^CTAGC
NspI 1233 GCATGT [GA]CATG^[CT]
PflFI 988 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3754 CACGTG CAC^GTG
PmlI 3754 CACGTG CAC^GTG
SacII 2116 CCGCGG CCGC^GG
SapI 1393 GCTCTTC GCTCTTC(1/4)
SbfI 729 CCTGCAGG CCTGCA^GG
SfoI 3425 GGCGCC GGC^GCC
SmaI 2763 CCCGGG CCC^GGG
Sse8387I 729 CCTGCAGG CCTGCA^GG
SspI 1900 AATATT AAT^ATT
Tth111I 988 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2763 CCCGGG C^CCGGG
XmaI 2763 CCCGGG C^CCGGG
XmnI 2775 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccII 407 CGCG CG^CG
2117 CGCG
AccIII 1934 TCCGGA T^CCGGA
3863 TCCGGA
AcyI 3425 GGCGCC G[GA]^CG[CT]C
3612 GACGTC
AhdI 1595 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3079 GACCAGCTGTC
AspEI 1595 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3079 GACCAGCTGTC
BbsI 3832 GAAGAC GAAGAC(2/6)
1079 GTCTTC
BpuAI 3832 GAAGAC GAAGAC(2/6)
1079 GTCTTC
BsaAI 1648 TACGTG [CT]AC^GT[GA]
3754 CACGTG
BsaHI 3425 GGCGCC G[GA]^CG[CT]C
3612 GACGTC
BsaWI 1934 TCCGGA [AT]^CCGG[AT]
3863 TCCGGA
BseAI 1934 TCCGGA T^CCGGA
3863 TCCGGA
BsmI 1525 GAATGC GAATGC(1/-1)
1722 GCATTC
BspEI 1934 TCCGGA T^CCGGA
3863 TCCGGA
BsrBI 145 CCGCTC CCGCTC(-3/-3)
1160 GAGCGG
BstUI 407 CGCG CG^CG
2117 CGCG
Eam1105I 1595 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3079 GACCAGCTGTC
EcoNI 252 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2478 CCTGTCAGAGG
HgaI 3711 GCGTC GACGC(5/10)
4119 GCGTC
Hin1I 3425 GGCGCC G[GA]^CG[CT]C
3612 GACGTC
MroI 1934 TCCGGA T^CCGGA
3863 TCCGGA
MseI 1303 TTAA T^TAA
2702 TTAA
MvnI 407 CGCG CG^CG
2117 CGCG
StuI 2420 AGGCCT AGG^CCT
3140 AGGCCT
Tru9I 1303 TTAA T^TAA
2702 TTAA
XcmI 1739 CCACAGGGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3365 CCACAGACACCATGG
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# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII