Restriction map for
pFN21AE1023
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# NO CUT ENZYMES ( 117 enzymes) #
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Acc65I AccII AccIII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BbeI BbrPI BciVI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NarI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 1173 GACGTC GACGT^C
AcsI 554 AAATTC [GA]^AATT[CT]
AflIII 2021 ACGTGT A^C[GA][CT]GT
ApoI 554 AAATTC [GA]^AATT[CT]
BalI 375 TGGCCA TGG^CCA
BamHI 2226 GGATCC G^GATCC
BbsI 196 GAAGAC GAAGAC(2/6)
BcgI 1948 GCAGCTAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1559 TGATCA T^GATCA
BglI 894 GCCTCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 582 AGATCT A^GATCT
BpuAI 196 GAAGAC GAAGAC(2/6)
BsgI 314 GTGCAG GTGCAG(16/14)
BsmBI 2018 GAGACG CGTCTC(1/5)
BspHI 2163 TCATGA T^CATGA
BsrBI 2252 CCGCTC CCGCTC(-3/-3)
BsrFI 507 GCCGGC [GA]^CCGG[CT]
BssSI 108 CTCGTG CACGAG(-5/-1)
BstAPI 1845 GCAGATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 732 CCAAGTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 2209 GCGC GCG^C
Cfr10I 507 GCCGGC [GA]^CCGG[CT]
EarI 613 GAAGAG CTCTTC(1/4)
EcoNI 994 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 53 CCATGG C^C[AT][AT]GG
FbaI 1559 TGATCA T^GATCA
HaeII 2208 AGCGCC [GA]GCGC^[CT]
HgaI 2275 GACGC GACGC(5/10)
HhaI 2209 GCGC GCG^C
HinP1I 2209 GCGC G^CGC
HindIII 2062 AAGCTT A^AGCTT
Ksp632I 613 GAAGAG CTCTTC(1/4)
MluNI 375 TGGCCA TGG^CCA
MscI 375 TGGCCA TGG^CCA
MslI 1557 CATGATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 507 GCCGGC GCC^GGC
NcoI 53 CCATGG C^CATGG
NgoMIV 507 GCCGGC G^CCGGC
NheI 1951 GCTAGC G^CTAGC
PflMI 2118 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 2163 TCATGA T^CATGA
SapI 613 GAAGAGC GCTCTTC(1/4)
ScaI 304 AGTACT AGT^ACT
SphI 35 GCATGC GCATG^C
StyI 53 CCATGG C^C[AT][AT]GG
TaqI 787 TCGA T^CGA
TthHB8I 787 TCGA T^CGA
Van91I 2118 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 889 CCAGTGCCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 514 GTAGAC GT^[AC][GT]AC
2047 GTAGAC
AcyI 1173 GACGTC G[GA]^CG[CT]C
2275 GACGCC
AlwNI 1748 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
2119 CAGGCCCTG
AvaI 2095 CTCGGG C^[CT]CG[GA]G
2174 CCCGAG
BanI 2078 GGCACC G^G[CT][GA]CC
2247 GGTGCC
BbvCI 1745 CCTCAGC CCTCAGC(-5/-2)
2008 CCTCAGC
BcnI 1654 CCGGG CC^[GC]GG
2146 CCCGG
BsaHI 1173 GACGTC G[GA]^CG[CT]C
2275 GACGCC
BsmAI 186 GAGAC GTCTC(1/5)
2018 GAGAC
BsoBI 2095 CTCGGG C^[CT]CG[GA]G
2174 CCCGAG
EaeI 375 TGGCCA [CT]^GGCC[GA]
1766 CGGCCA
FauI 1201 CCCGC CCCGC(4/6)
2251 CCCGC
Hin1I 1173 GACGTC G[GA]^CG[CT]C
2275 GACGCC
MaeII 1174 ACGT A^CGT
2021 ACGT
NciI 1654 CCGGG CC^[GC]GG
2146 CCCGG
PpuMI 326 AGGTCCC [GA]G^G[AT]CC[CT]
1656 GGGTCCC
SmlI 586 CTTGAG C^T[CT][GA]AG
1316 CTCAAG
Tsp509I 246 AATT ^AATT
555 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AfaI AluI AlwI ApaI
AspHI AvaII BanII BbvI BfaI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII DsaI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI TfiI Tru9I
TseI Tsp45I TspRI XhoII