(Circular) (Six-base) MAPSORT of: pFN21AE1023.seq Check: 4455 from: 1 to: 7027 pFN21AE1023 7027 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4240 4240 Size: 7027 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3182 4837 278 Size: 53 83 186 936 1646 1655 2468 Fragments arranged by size: 2468 1655 1646 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2520 4053 4370 1937 Size: 583 1533 317 4594 Fragments arranged by size: 4594 1533 583 317 Acc65I G'GTAC_C Cuts at: 4348 4348 Size: 7027 AclI AA'CG_TT Cuts at: 1563 5154 5527 1563 Size: 3591 373 3063 Fragments arranged by size: 3591 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2741 2945 5090 6474 6742 1632 Size: 1109 204 2145 1384 268 1917 Fragments arranged by size: 2145 1917 1384 1109 268 204 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6830 AflIII A'CryG_T Cuts at: 1203 4026 5926 6903 1203 Size: 2823 1900 977 1327 Fragments arranged by size: 2823 1900 1327 977 AgeI A'CCGG_T Cuts at: 5858 5858 Size: 7027 AhdI GACnn_n'nnGTC Cuts at: 5756 5756 Size: 7027 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7027 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2970 3584 1184 Size: 1786 614 4627 Fragments arranged by size: 4627 1786 614 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2316 2348 2316 Size: 32 6995 AlwNI CAG_nnn'CTG Cuts at: 3758 4129 4770 6342 3758 Size: 371 641 1572 4443 Fragments arranged by size: 4443 1572 641 371 ApaI G_GGCC'C Cuts at: 2294 2478 2976 3207 2294 Size: 184 498 231 6114 Fragments arranged by size: 6114 498 231 184 ApaLI G'TGCA_C Cuts at: 5084 6240 5084 Size: 1156 5871 ApoI r'AATT_y Cuts at: 1493 2559 4336 4562 4714 6823 1493 Size: 1066 1777 226 152 2109 1697 Fragments arranged by size: 2109 1777 1697 1066 226 152 AseI AT'TA_AT Cuts at: 160 1032 5581 160 Size: 872 4549 1606 Fragments arranged by size: 4549 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7027 AvaI C'yCGr_G Cuts at: 1870 1943 4100 4179 4352 1870 Size: 73 2157 79 173 4545 Fragments arranged by size: 4545 2157 173 79 73 BamHI G'GATC_C Cuts at: 4231 4357 4724 4231 Size: 126 367 6534 Fragments arranged by size: 6534 367 126 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4083 4252 4348 5803 618 Size: 325 217 627 2296 169 96 1455 1842 Fragments arranged by size: 2296 1842 1455 627 325 217 169 96 BanII G_rGCy'C Cuts at: 1391 2082 2294 2432 2478 2553 2976 3207 3281 Size: 691 212 138 46 75 423 231 74 Cuts at: 3281 3992 4346 1391 Size: 711 354 4072 Fragments arranged by size: 4072 711 691 423 354 231 212 138 75 74 46 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2208 928 Size: 971 309 5747 Fragments arranged by size: 5747 971 309 BbvCI CC'TCA_GC Cuts at: 3751 4014 3751 Size: 263 6764 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3942 3976 5218 5252 1903 Size: 34 2005 34 1242 34 3678 Fragments arranged by size: 3678 2005 1242 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4919 6129 4919 Size: 1210 5817 BclI T'GATC_A Cuts at: 1548 3564 1548 Size: 2016 5011 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2905 5638 136 Size: 107 122 71 631 605 1233 2733 1525 Fragments arranged by size: 2733 1525 1233 631 605 122 107 71 BglII A'GATC_T Cuts at: 2587 7022 2587 Size: 4435 2592 BlpI GC'TnA_GC Cuts at: 4444 4444 Size: 7027 Bme1580I G_kGCm'C Cuts at: 1790 2294 2478 2976 3207 4257 5088 6244 1790 Size: 504 184 498 231 1050 831 1156 2573 Fragments arranged by size: 2573 1156 1050 831 504 498 231 184 BmgBI CAC'GTC Cuts at: 1318 4027 1318 Size: 2709 4318 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2612 2976 3023 Size: 416 53 83 399 24 1195 364 47 Cuts at: 3023 4758 5716 442 Size: 1735 958 1753 Fragments arranged by size: 1753 1735 1195 958 416 399 364 83 53 47 24 BmtI G_CTAG'C Cuts at: 1056 3960 1056 Size: 2904 4123 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3409 3409 Size: 7027 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2075 2417 2988 3574 4041 4264 5687 1239 Size: 836 342 571 586 467 223 1423 2579 Fragments arranged by size: 2579 1423 836 586 571 467 342 223 Bpu10I CC'TnA_GC Cuts at: 2654 3557 3751 4014 2654 Size: 903 194 263 5667 Fragments arranged by size: 5667 903 263 194 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2612 3306 5152 6017 6315 2612 Size: 694 1846 865 298 3324 Fragments arranged by size: 3324 1846 865 694 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5690 882 Size: 617 4191 2219 Fragments arranged by size: 4191 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4818 493 Size: 711 3614 2702 Fragments arranged by size: 3614 2702 711 BsaBI GATnn'nnATC Cuts at: 7021 7021 Size: 7027 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3179 4281 4834 5216 Size: 53 83 186 936 1646 1102 553 382 Cuts at: 5216 5851 275 Size: 635 1451 Fragments arranged by size: 1646 1451 1102 936 635 553 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5460 5858 6132 6279 6690 5460 Size: 398 274 147 411 5797 Fragments arranged by size: 5797 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6802 Fragments arranged by size: 6802 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2203 2561 2761 2991 3739 3754 4180 1186 Size: 1017 358 200 230 748 15 426 4033 Fragments arranged by size: 4033 1017 748 426 358 230 200 15 BseYI C'CCAG_C Cuts at: 3236 3867 3972 4227 6230 6726 3236 Size: 631 105 255 2003 496 3537 Fragments arranged by size: 3537 2003 631 496 255 105 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2340 1163 Size: 573 604 5850 Fragments arranged by size: 5850 604 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4477 5238 6266 1540 Size: 275 186 2476 761 1028 2301 Fragments arranged by size: 2476 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2302 2553 3281 3992 5088 5173 6244 2302 Size: 251 728 711 1096 85 1071 3085 Fragments arranged by size: 3085 1096 1071 728 711 251 85 BsmI GAATG_Cn' Cuts at: 4538 4631 4538 Size: 93 6934 BsmBI CGTCTCn'nnnn_ Cuts at: 4017 4017 Size: 7027 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2082 2294 2302 2432 2478 2553 2976 Size: 399 292 212 8 130 46 75 423 Cuts at: 2976 3207 3281 3992 4257 4346 5088 5173 6244 Size: 231 74 711 265 89 742 85 1071 Cuts at: 6244 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 711 423 399 292 265 231 212 130 89 85 75 74 46 8 BspEI T'CCGG_A Cuts at: 6690 6690 Size: 7027 BspHI T'CATG_A Cuts at: 4168 4916 6646 4168 Size: 748 1730 4549 Fragments arranged by size: 4549 1730 748 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2320 3729 4240 4382 844 Size: 1476 1409 511 142 3489 Fragments arranged by size: 3489 1476 1409 511 142 BsrBI CCG'CTC Cuts at: 1394 1823 4259 4914 1394 Size: 429 2436 655 3507 Fragments arranged by size: 3507 2436 655 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2292 3196 4324 5522 5696 65 Size: 1183 1044 904 1128 1198 174 1396 Fragments arranged by size: 1396 1198 1183 1128 1044 904 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2512 5671 5858 1700 Size: 110 702 3159 187 2869 Fragments arranged by size: 3159 2869 702 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7027 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7027 BssSI C'ACGA_G Cuts at: 2113 5087 6099 2113 Size: 2974 1012 3041 Fragments arranged by size: 3041 2974 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3856 794 Size: 3062 3965 BstBI TT'CG_AA Cuts at: 5831 5831 Size: 7027 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7027 BstXI CCAn_nnnn'nTGG Cuts at: 2744 2744 Size: 7027 BstYI r'GATC_y Cuts at: 1972 2587 2760 4009 4231 4357 4724 5109 5126 Size: 615 173 1249 222 126 367 385 17 Cuts at: 5126 6578 6664 6676 7022 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1249 615 385 367 346 222 173 126 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2058 3603 3768 513 Size: 552 939 54 1545 165 3772 Fragments arranged by size: 3772 1545 939 552 165 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6288 Fragments arranged by size: 6288 683 56 BtsI GCAGTG_nn' Cuts at: 3059 3756 3774 4543 5337 5357 3059 Size: 697 18 769 794 20 4729 Fragments arranged by size: 4729 794 769 697 20 18 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7027 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6992 DraI TTT'AAA Cuts at: 4331 4331 Size: 7027 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6034 809 Size: 507 4718 1802 Fragments arranged by size: 4718 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2380 3771 4474 Size: 54 1425 50 275 18 550 1391 703 Cuts at: 4474 5363 6992 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1391 889 703 550 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4474 1537 Size: 275 2662 4090 Fragments arranged by size: 4090 2662 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2613 4957 1353 Size: 1260 2344 3423 Fragments arranged by size: 3423 2344 1260 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5614 5988 6134 387 Size: 1064 138 4025 374 146 1280 Fragments arranged by size: 4025 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2551 3279 3990 2551 Size: 728 711 5588 Fragments arranged by size: 5588 728 711 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 2417 2741 2945 2988 3574 4041 Size: 393 443 342 324 204 43 586 467 Cuts at: 4041 4264 5090 5687 6474 6742 1239 Size: 223 826 597 787 268 1524 Fragments arranged by size: 1524 826 787 597 586 467 443 393 342 324 268 223 204 43 EcoNI CCTnn'n_nnAGG Cuts at: 3003 3003 Size: 7027 EcoO109I rG'GnC_Cy Cuts at: 2332 2474 2972 3203 3662 4126 2332 Size: 142 498 231 459 464 5233 Fragments arranged by size: 5233 498 464 459 231 142 EcoRI G'AATT_C Cuts at: 4336 4714 4336 Size: 378 6649 FspI TGC'GCA Cuts at: 5533 5533 Size: 7027 HaeII r_GCGC'y Cuts at: 1678 4217 6174 1678 Size: 2539 1957 2531 Fragments arranged by size: 2539 2531 1957 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2335 2367 3325 3357 4292 Size: 32 135 32 763 32 958 32 935 Cuts at: 4292 4324 5668 5700 5742 5774 1373 Size: 32 1344 32 42 32 2626 Fragments arranged by size: 2626 1344 958 935 763 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4371 4617 669 Size: 988 281 2433 246 3079 Fragments arranged by size: 3079 2433 988 281 246 HindIII A'AGCT_T Cuts at: 748 4067 748 Size: 3319 3708 HpaI GTT'AAC Cuts at: 4617 4617 Size: 7027 KpnI G_GTAC'C Cuts at: 4352 4352 Size: 7027 MfeI C'AATT_G Cuts at: 4626 4626 Size: 7027 MluI A'CGCG_T Cuts at: 6903 6903 Size: 7027 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6141 6325 1305 Size: 4836 184 2007 Fragments arranged by size: 4836 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2382 10 Size: 54 1425 343 550 4655 Fragments arranged by size: 4655 1425 550 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3566 4985 5344 5503 518 Size: 648 2400 1419 359 159 2042 Fragments arranged by size: 2400 2042 1419 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2923 3055 3790 4227 4443 5120 6268 6513 Size: 1317 132 735 437 216 677 1148 245 Cuts at: 6513 1606 Size: 2120 Fragments arranged by size: 2120 1317 1148 735 677 437 245 216 132 NaeI GCC'GGC Cuts at: 2514 2514 Size: 7027 NcoI C'CATG_G Cuts at: 513 1065 2004 2058 513 Size: 552 939 54 5482 Fragments arranged by size: 5482 939 552 54 NdeI CA'TA_TG Cuts at: 387 387 Size: 7027 NgoMIV G'CCGG_C Cuts at: 2512 2512 Size: 7027 NheI G'CTAG_C Cuts at: 1052 3956 1052 Size: 2904 4123 NotI GC'GGCC_GC Cuts at: 4474 4474 Size: 7027 NspI r_CATG'y Cuts at: 1132 1229 2044 2752 3115 4385 5930 1132 Size: 97 815 708 363 1270 1545 2229 Fragments arranged by size: 2229 1545 1270 815 708 363 97 PasI CC'CwG_GG Cuts at: 1348 3199 3359 4129 4218 1348 Size: 1851 160 770 89 4157 Fragments arranged by size: 4157 1851 770 160 89 PciI A'CATG_T Cuts at: 5926 5926 Size: 7027 PflMI CCAn_nnn'nTGG Cuts at: 1108 4129 1108 Size: 3021 4006 PfoI T'CCnGG_A Cuts at: 2091 3004 2091 Size: 913 6114 PmeI GTTT'AAAC Cuts at: 4331 4331 Size: 7027 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5096 5128 6609 6641 5096 Size: 32 1481 32 5482 Fragments arranged by size: 5482 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2332 3662 2332 Size: 1330 5697 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7027 PsiI TTA'TAA Cuts at: 4597 4597 Size: 7027 PspOMI G'GGCC_C Cuts at: 2290 2474 2972 3203 2290 Size: 184 498 231 6114 Fragments arranged by size: 6114 498 231 184 PstI C_TGCA'G Cuts at: 830 2331 3726 3795 3849 3955 4379 830 Size: 1501 1395 69 54 106 424 3478 Fragments arranged by size: 3478 1501 1395 424 106 69 54 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7027 PvuII CAG'CTG Cuts at: 2923 3790 4227 2923 Size: 867 437 5723 Fragments arranged by size: 5723 867 437 SacI G_AGCT'C Cuts at: 2553 3281 3992 2553 Size: 728 711 5588 Fragments arranged by size: 5588 728 711 SalI G'TCGA_C Cuts at: 1936 4369 1936 Size: 2433 4594 SanDI GG'GwC_CC Cuts at: 3662 3662 Size: 7027 SapI GCTCTTCn'nnn_ Cuts at: 2613 2613 Size: 7027 SbfI CC_TGCA'GG Cuts at: 4379 4379 Size: 7027 ScaI AGT'ACT Cuts at: 2311 5275 6987 2311 Size: 2964 1712 2351 Fragments arranged by size: 2964 2351 1712 SfcI C'TryA_G Cuts at: 826 1045 2327 2454 2517 3722 3791 3845 3951 Size: 219 1282 127 63 1205 69 54 106 Cuts at: 3951 4375 5510 6191 6382 826 Size: 424 1135 681 191 1471 Fragments arranged by size: 1471 1282 1205 1135 681 424 219 191 127 106 69 63 54 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7027 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7027 SmaI CCC'GGG Cuts at: 1872 4354 1872 Size: 2482 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2591 3321 5131 6032 6294 820 Size: 197 926 648 730 1810 901 262 1553 Fragments arranged by size: 1810 1553 926 901 730 648 262 197 SnaBI TAC'GTA Cuts at: 493 4818 493 Size: 4325 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7027 SphI G_CATG'C Cuts at: 1132 2044 4385 1132 Size: 912 2341 3774 Fragments arranged by size: 3774 2341 912 SspI AAT'ATT Cuts at: 5 52 4951 5 Size: 47 4899 2081 Fragments arranged by size: 4899 2081 47 StuI AGG'CCT Cuts at: 1517 2260 3746 4241 1517 Size: 743 1486 495 4303 Fragments arranged by size: 4303 1486 743 495 StyI C'CwwG_G Cuts at: 513 1065 2004 2058 4466 513 Size: 552 939 54 2408 3074 Fragments arranged by size: 3074 2408 939 552 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3060 5054 5071 5224 5409 5854 325 Size: 1255 1480 1994 17 153 185 445 1498 Fragments arranged by size: 1994 1498 1480 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2309 5273 6985 Size: 274 80 33 51 1443 332 2964 1712 Cuts at: 6985 96 Size: 138 Fragments arranged by size: 2964 1712 1443 332 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2745 2777 6987 7019 2745 Size: 32 4210 32 2753 Fragments arranged by size: 4210 2753 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7027 XbaI T'CTAG_A Cuts at: 4363 4363 Size: 7027 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2901 2901 Size: 7027 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7027 XmaI C'CCGG_G Cuts at: 1870 4352 1870 Size: 2482 4545 XmnI GAAnn'nnTTC Cuts at: 5156 5156 Size: 7027 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3180 4835 276 Size: 53 83 186 936 1646 1655 2468 Fragments arranged by size: 2468 1655 1646 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI