Restriction map for
pFN21AE1022
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# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AocI ApoI AscI
AseI AsnI Asp718I AvrII BbeI
BbrPI BcgI BciVI BfrI BglI
BglII BlnI BsaBI BsiWI Bsp1407I
BspDI BsrGI BssHII Bst1107I BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DraIII Eco81I EcoO65I EcoRI
EcoRV EcoT22I FseI HincII HindII
HindIII HpaI KasI KpnI MamI
MfeI MluI MunI NarI NdeI
NheI NotI NruI NsiI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PstI PvuI
RsrII SacI SalI SbfI ScaI
SfiI SfoI SgrAI SpeI SphI
Sse8387I SspBI SspI SwaI XbaI
XhoI
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# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccIII 315 TCCGGA T^CCGGA
AflIII 883 ACATGT A^C[GA][CT]GT
AhdI 1986 GACATCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 799 GTGCAC G^TGCAC
ApaI 2070 GGGCCC GGGCC^C
ApaLI 799 GTGCAC G^TGCAC
AspEI 1986 GACATCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1235 TGCGCA TGC^GCA
BalI 1964 TGGCCA TGG^CCA
BamHI 1611 GGATCC G^GATCC
BclI 728 TGATCA T^GATCA
BfaI 1450 CTAG C^TAG
BlpI 297 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 297 GCTGAGC GC^T[ACGT]AGC
BsaAI 609 TACGTA [CT]AC^GT[GA]
BseAI 315 TCCGGA T^CCGGA
BsgI 1345 CTGCAC GTGCAG(16/14)
BsiEI 2093 CGGCCG CG[GA][CT]^CG
BsmI 1644 GAATGC GAATGC(1/-1)
BspEI 315 TCCGGA T^CCGGA
BspHI 1415 TCATGA T^CATGA
BspLU11I 883 ACATGT A^CATGT
BsrDI 248 CATTGC GCAATG(2/0)
BssSI 643 CACGAG CACGAG(-5/-1)
BstAPI 2145 GCATGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1641 TTCGAA TT^CGAA
BstXI 440 CCATGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 297 GCTGAGC GC^T[ACGT]AGC
DrdI 1317 GACGGGATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2093 CGGCCG C^GGCCG
Eam1105I 1986 GACATCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2093 CGGCCG C^GGCCG
Eco52I 2093 CGGCCG C^GGCCG
EcoNI 447 CCTGGTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 728 TGATCA T^GATCA
FspI 1235 TGCGCA TGC^GCA
KspI 1616 CCGCGG CCGC^GG
MaeI 1450 CTAG C^TAG
MluNI 1964 TGGCCA TGG^CCA
MroI 315 TCCGGA T^CCGGA
MscI 1964 TGGCCA TGG^CCA
MseI 10 TTAA T^TAA
MslI 1359 CATTGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspV 1641 TTCGAA TT^CGAA
PflMI 2162 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1908 GACGGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1415 TCATGA T^CATGA
SacII 1616 CCGCGG CCGC^GG
SapI 2106 GAAGAGC GCTCTTC(1/4)
SexAI 446 ACCTGGT A^CC[AT]GGT
SfuI 1641 TTCGAA TT^CGAA
SmlI 1531 CTCAAG C^T[CT][GA]AG
SnaBI 609 TACGTA TAC^GTA
StuI 434 AGGCCT AGG^CCT
Tru9I 10 TTAA T^TAA
Van91I 2162 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1653 CCAGTACAAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccII 1617 CGCG CG^CG
2091 CGCG
AcyI 457 GACGCC G[GA]^CG[CT]C
1554 GGCGTC
AfaI 612 GTAC GT^AC
1656 GTAC
AlwNI 715 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1077 CAGCCGCTG
Aor51HI 1091 AGCGCT AGC^GCT
1935 AGCGCT
Asp700I 489 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1036 GAAGATGTTC
AspHI 799 GTGCAC G[AT]GC[AT]^C
1066 GTGCTC
AspI 445 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1504 GACGATGTC
AvaI 1194 CCCGGG C^[CT]CG[GA]G
2121 CCCGGG
BsaHI 457 GACGCC G[GA]^CG[CT]C
1554 GGCGTC
BsaI 160 GAGACC GGTCTC(1/5)
837 GAGACC
BsaWI 315 TCCGGA [AT]^CCGG[AT]
2018 ACCGGA
BsiHKAI 799 GTGCAC G[AT]GC[AT]^C
1066 GTGCTC
BsmBI 25 CGTCTC CGTCTC(1/5)
1315 GAGACG
BsoBI 1194 CCCGGG C^[CT]CG[GA]G
2121 CCCGGG
BspMI 1048 ACCTGC ACCTGC(4/8)
1289 GCAGGT
BsrBI 1171 CCGCTC CCGCTC(-3/-3)
1787 GAGCGG
BstUI 1617 CGCG CG^CG
2091 CGCG
BstYI 206 AGATCC [GA]^GATC[CT]
1611 GGATCC
Eco47III 1091 AGCGCT AGC^GCT
1935 AGCGCT
HaeII 1091 AGCGCT [GA]GCGC^[CT]
1935 AGCGCT
Hin1I 457 GACGCC G[GA]^CG[CT]C
1554 GGCGTC
MflI 206 AGATCC [GA]^GATC[CT]
1611 GGATCC
MvnI 1617 CGCG CG^CG
2091 CGCG
NaeI 1807 GCCGGC GCC^GGC
1865 GCCGGC
NcoI 440 CCATGG C^CATGG
1550 CCATGG
NgoMIV 1807 GCCGGC G^CCGGC
1865 GCCGGC
NspI 883 ACATGT [GA]CATG^[CT]
2145 GCATGT
PflFI 445 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1504 GACGATGTC
RsaI 612 GTAC GT^AC
1656 GTAC
SfcI 741 CTACAG C^T[GA][CT]AG
1699 CTACAG
SmaI 1194 CCCGGG CCC^GGG
2121 CCCGGG
Tth111I 445 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1504 GACGATGTC
XhoII 206 AGATCC [GA]^GATC[CT]
1611 GGATCC
XmaCI 1194 CCCGGG C^CCGGG
2121 CCCGGG
XmaI 1194 CCCGGG C^CCGGG
2121 CCCGGG
XmnI 489 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1036 GAAGATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AluI AlwI AvaII BanI
BanII BbsI BbvCI BbvI BcnI
BmyI BpmI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PvuII Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I