(Circular) (Six-base) MAPSORT of: pFN21AE1022.seq Check: 4071 from: 1 to: 6895 pFN21AE1022 6895 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3062 3285 3062 Size: 223 6672 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4705 278 Size: 53 83 186 936 3169 2468 Fragments arranged by size: 3169 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4238 1937 Size: 2301 4594 Acc65I G'GTAC_C Cuts at: 4216 4216 Size: 6895 AclI AA'CG_TT Cuts at: 1563 5022 5395 1563 Size: 3459 373 3063 Fragments arranged by size: 3459 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2675 2747 2820 3173 3303 3749 3869 3953 Size: 1043 72 73 353 130 446 120 84 Cuts at: 3953 4958 6342 6610 1632 Size: 1005 1384 268 1917 Fragments arranged by size: 1917 1384 1043 1005 446 353 268 130 120 84 73 72 AfeI AGC'GCT Cuts at: 3098 3942 3098 Size: 844 6051 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6698 AflIII A'CryG_T Cuts at: 1203 2888 5794 6771 1203 Size: 1685 2906 977 1327 Fragments arranged by size: 2906 1685 1327 977 AgeI A'CCGG_T Cuts at: 5726 5726 Size: 6895 AhdI GACnn_n'nnGTC Cuts at: 3996 5624 3996 Size: 1628 5267 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6895 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6895 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2580 2612 2908 2940 3059 3091 2580 Size: 32 296 32 119 32 6384 Fragments arranged by size: 6384 296 119 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 2725 3087 4638 6210 2725 Size: 362 1551 1572 3410 Fragments arranged by size: 3410 1572 1551 362 ApaI G_GGCC'C Cuts at: 4079 4079 Size: 6895 ApaLI G'TGCA_C Cuts at: 2804 4952 6108 2804 Size: 2148 1156 3591 Fragments arranged by size: 3591 2148 1156 ApoI r'AATT_y Cuts at: 1493 4204 4430 4582 6691 1493 Size: 2711 226 152 2109 1697 Fragments arranged by size: 2711 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5449 160 Size: 872 4417 1606 Fragments arranged by size: 4417 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6895 AvaI C'yCGr_G Cuts at: 1870 1943 3199 4126 4220 1870 Size: 73 1256 927 94 4545 Fragments arranged by size: 4545 1256 927 94 73 BamHI G'GATC_C Cuts at: 3616 4225 4592 3616 Size: 609 367 5919 Fragments arranged by size: 5919 609 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2418 3102 3461 4020 4216 Size: 325 217 627 631 684 359 559 196 Cuts at: 4216 5671 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 684 631 627 559 359 325 217 196 BanII G_rGCy'C Cuts at: 1391 2309 3981 4079 4214 1391 Size: 918 1672 98 135 4072 Fragments arranged by size: 4072 1672 918 135 98 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2826 3161 3291 3593 928 Size: 971 927 335 130 302 4230 Fragments arranged by size: 4230 971 927 335 302 130 BbvCI CC'TCA_GC Cuts at: 2217 3031 3036 2217 Size: 814 5 6076 Fragments arranged by size: 6076 814 5 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5086 5120 1903 Size: 34 3149 34 3678 Fragments arranged by size: 3678 3149 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4787 5997 4787 Size: 1210 5685 BclI T'GATC_A Cuts at: 1548 2733 1548 Size: 1185 5710 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5506 136 Size: 107 122 71 631 605 3834 1525 Fragments arranged by size: 3834 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6890 6890 Size: 6895 BlpI GC'TnA_GC Cuts at: 2303 4312 2303 Size: 2009 4886 Bme1580I G_kGCm'C Cuts at: 1790 2675 2808 2948 3464 4079 4956 6112 1790 Size: 885 133 140 516 615 877 1156 2573 Fragments arranged by size: 2573 1156 885 877 615 516 140 133 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6895 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2973 3652 4626 Size: 416 53 83 399 24 1556 679 974 Cuts at: 4626 5584 442 Size: 958 1753 Fragments arranged by size: 1753 1556 974 958 679 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6895 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2458 3043 2458 Size: 585 6310 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2840 3857 3878 3920 3926 3993 4033 5555 Size: 1601 1017 21 42 6 67 40 1522 Cuts at: 5555 1239 Size: 2579 Fragments arranged by size: 2579 1601 1522 1017 67 42 40 21 6 Bpu10I CC'TnA_GC Cuts at: 2217 3031 3036 2217 Size: 814 5 6076 Fragments arranged by size: 6076 814 5 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3521 5020 5885 6183 3521 Size: 1499 865 298 4233 Fragments arranged by size: 4233 1499 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2159 2836 5558 882 Size: 617 660 677 2722 2219 Fragments arranged by size: 2722 2219 677 660 617 BsaAI yAC'GTr Cuts at: 493 1204 2616 4686 493 Size: 711 1412 2070 2702 Fragments arranged by size: 2702 2070 1412 711 BsaBI GATnn'nnATC Cuts at: 6889 6889 Size: 6895 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2463 3560 4702 5084 Size: 53 83 186 936 930 1097 1142 382 Cuts at: 5084 5719 275 Size: 635 1451 Fragments arranged by size: 1451 1142 1097 936 930 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2320 4023 5328 5726 6000 6147 6558 2320 Size: 1703 1305 398 274 147 411 2657 Fragments arranged by size: 2657 1703 1305 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2580 2610 3059 3089 3851 Size: 30 165 30 1177 30 449 30 762 Cuts at: 3851 3881 1178 Size: 30 4192 Fragments arranged by size: 4192 1177 762 449 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2181 2402 2405 2450 2775 3019 3731 3734 Size: 995 221 3 45 325 244 712 3 Cuts at: 3734 3854 3875 3878 1186 Size: 120 21 3 4203 Fragments arranged by size: 4203 995 712 325 244 221 120 45 21 3 3 3 BseYI C'CCAG_C Cuts at: 2846 3485 3762 6098 6594 2846 Size: 639 277 2336 496 3147 Fragments arranged by size: 3147 2336 639 496 277 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3335 1163 Size: 573 1599 4723 Fragments arranged by size: 4723 1599 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4101 4345 5106 6134 1540 Size: 275 186 2100 244 761 1028 2301 Fragments arranged by size: 2301 2100 1028 761 275 244 186 BsiHKAI G_wGCw'C Cuts at: 2808 3075 4956 5041 6112 2808 Size: 267 1881 85 1071 3591 Fragments arranged by size: 3591 1881 1071 267 85 BsmI GAATG_Cn' Cuts at: 3655 4406 4499 3655 Size: 751 93 6051 Fragments arranged by size: 6051 751 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2036 3314 2036 Size: 1278 5617 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2309 2675 2808 2948 3075 3464 3981 Size: 399 519 366 133 140 127 389 517 Cuts at: 3981 4079 4214 4956 5041 6112 1391 Size: 98 135 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 519 517 399 389 366 140 135 133 127 98 85 BspEI T'CCGG_A Cuts at: 2320 6558 2320 Size: 4238 2657 BspHI T'CATG_A Cuts at: 3420 4784 6514 3420 Size: 1364 1730 3801 Fragments arranged by size: 3801 1730 1364 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3062 3285 4250 844 Size: 2218 223 965 3489 Fragments arranged by size: 3489 2218 965 223 BsrBI CCG'CTC Cuts at: 1394 1823 3178 3794 4782 1394 Size: 429 1355 616 988 3507 Fragments arranged by size: 3507 1355 988 616 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2252 5390 5564 65 Size: 1183 1004 3138 174 1396 Fragments arranged by size: 3138 1396 1183 1004 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3812 3870 4084 5539 5726 1700 Size: 110 2002 58 214 1455 187 2869 Fragments arranged by size: 2869 2002 1455 214 187 110 58 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6895 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6895 BssSI C'ACGA_G Cuts at: 2648 4955 5967 2648 Size: 2307 1012 3576 Fragments arranged by size: 3576 2307 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4156 794 Size: 3362 3533 BstBI TT'CG_AA Cuts at: 3647 5699 3647 Size: 2052 4843 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6895 BstXI CCAn_nnnn'nTGG Cuts at: 2452 2452 Size: 6895 BstYI r'GATC_y Cuts at: 1972 2211 3616 4225 4592 4977 4994 6446 6532 Size: 239 1405 609 367 385 17 1452 86 Cuts at: 6532 6544 6890 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1405 609 385 367 346 239 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2445 2766 3181 3555 3621 513 Size: 552 939 441 321 415 374 66 3787 Fragments arranged by size: 3787 939 552 441 415 374 321 66 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2369 487 Size: 683 56 1143 5013 Fragments arranged by size: 5013 1143 683 56 BtsI GCAGTG_nn' Cuts at: 2097 2726 4411 5205 5225 2097 Size: 629 1685 794 20 3767 Fragments arranged by size: 3767 1685 794 629 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6895 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6860 DraI TTT'AAA Cuts at: 4199 4199 Size: 6895 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3328 5902 809 Size: 507 2012 2574 1802 Fragments arranged by size: 2574 2012 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2503 3247 3969 Size: 54 1425 50 275 18 673 744 722 Cuts at: 3969 4098 4342 5231 6860 8 Size: 129 244 889 1629 43 Fragments arranged by size: 1629 1425 889 744 722 673 275 244 129 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4098 4342 1537 Size: 275 2286 244 4090 Fragments arranged by size: 4090 2286 275 244 EarI CTCTTCn'nnn_ Cuts at: 1353 2004 2379 2906 3725 4106 4825 1353 Size: 651 375 527 819 381 719 3423 Fragments arranged by size: 3423 819 719 651 527 381 375 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3899 3966 5482 5856 6002 387 Size: 1064 138 2310 67 1516 374 146 1280 Fragments arranged by size: 2310 1516 1280 1064 374 146 138 67 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2675 2747 2820 2840 3173 3303 3749 Size: 393 1043 72 73 20 333 130 446 Cuts at: 3749 3857 3869 3878 3920 3926 3953 3993 4033 Size: 108 12 9 42 6 27 40 40 Cuts at: 4033 4958 5555 6342 6610 1239 Size: 925 597 787 268 1524 Fragments arranged by size: 1524 1043 925 787 597 446 393 333 268 130 108 73 72 42 40 40 27 20 12 9 6 EcoNI CCTnn'n_nnAGG Cuts at: 2456 2456 Size: 6895 EcoO109I rG'GnC_Cy Cuts at: 3742 4075 4076 3742 Size: 333 1 6561 Fragments arranged by size: 6561 333 1 EcoRI G'AATT_C Cuts at: 4204 4582 4204 Size: 378 6517 FspI TGC'GCA Cuts at: 3242 5401 3242 Size: 2159 4736 HaeII r_GCGC'y Cuts at: 1678 3100 3944 6042 1678 Size: 1422 844 2098 2531 Fragments arranged by size: 2531 2098 1422 844 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2974 3006 3311 3343 3968 Size: 32 135 32 1402 32 305 32 625 Cuts at: 3968 3982 4000 4014 5536 5568 5610 5642 1373 Size: 14 18 14 1522 32 42 32 2626 Fragments arranged by size: 2626 1522 1402 625 305 135 42 32 32 32 32 32 32 18 14 14 HincII GTy'rAC Cuts at: 669 1657 1938 4239 4485 669 Size: 988 281 2301 246 3079 Fragments arranged by size: 3079 2301 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6895 HpaI GTT'AAC Cuts at: 4485 4485 Size: 6895 KpnI G_GTAC'C Cuts at: 4220 4220 Size: 6895 MfeI C'AATT_G Cuts at: 4494 4494 Size: 6895 MluI A'CGCG_T Cuts at: 6771 6771 Size: 6895 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3102 3492 6009 6193 1305 Size: 1797 390 2517 184 2007 Fragments arranged by size: 2517 2007 1797 390 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3971 10 Size: 54 1425 343 2139 2934 Fragments arranged by size: 2934 2139 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3368 4853 5212 5371 518 Size: 648 2202 1485 359 159 2042 Fragments arranged by size: 2202 2042 1485 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2302 3035 3087 3489 3623 3856 4053 4311 Size: 696 733 52 402 134 233 197 258 Cuts at: 4311 4988 6136 6381 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 733 696 677 402 258 245 233 197 134 52 NaeI GCC'GGC Cuts at: 3814 3872 3814 Size: 58 6837 NcoI C'CATG_G Cuts at: 513 1065 2004 2445 3555 513 Size: 552 939 441 1110 3853 Fragments arranged by size: 3853 1110 939 552 441 NdeI CA'TA_TG Cuts at: 387 387 Size: 6895 NgoMIV G'CCGG_C Cuts at: 3812 3870 3812 Size: 58 6837 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6895 NotI GC'GGCC_GC Cuts at: 4342 4342 Size: 6895 NspI r_CATG'y Cuts at: 1132 1229 2892 4154 4253 5798 1132 Size: 97 1663 1262 99 1545 2229 Fragments arranged by size: 2229 1663 1545 1262 99 97 PasI CC'CwG_GG Cuts at: 1348 3961 1348 Size: 2613 4282 PciI A'CATG_T Cuts at: 2888 5794 2888 Size: 2906 3989 PflMI CCAn_nnn'nTGG Cuts at: 1108 4173 1108 Size: 3065 3830 PfoI T'CCnGG_A Cuts at: 2046 3834 2046 Size: 1788 5107 PmeI GTTT'AAAC Cuts at: 4199 4199 Size: 6895 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2016 2048 2580 2612 3059 3091 4964 4996 6477 Size: 32 532 32 447 32 1873 32 1481 Cuts at: 6477 6509 2016 Size: 32 2402 Fragments arranged by size: 2402 1873 1481 532 447 32 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3917 1613 Size: 2304 4591 PsiI TTA'TAA Cuts at: 4465 4465 Size: 6895 PspOMI G'GGCC_C Cuts at: 4075 4075 Size: 6895 PstI C_TGCA'G Cuts at: 830 4247 830 Size: 3417 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6895 PvuII CAG'CTG Cuts at: 3035 3856 4053 3035 Size: 821 197 5877 Fragments arranged by size: 5877 821 197 SacII CC_GC'GG Cuts at: 3624 3624 Size: 6895 SalI G'TCGA_C Cuts at: 1936 4237 1936 Size: 2301 4594 SapI GCTCTTCn'nnn_ Cuts at: 4106 4106 Size: 6895 SbfI CC_TGCA'GG Cuts at: 4247 4247 Size: 6895 ScaI AGT'ACT Cuts at: 5143 6855 5143 Size: 1712 5183 SexAI A'CCwGG_T Cuts at: 2451 2451 Size: 6895 SfcI C'TryA_G Cuts at: 826 1045 2746 3704 4243 5378 6059 6250 826 Size: 219 1701 958 539 1135 681 191 1471 Fragments arranged by size: 1701 1471 1135 958 681 539 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6895 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6895 SmaI CCC'GGG Cuts at: 1872 3201 4128 4222 1872 Size: 1329 927 94 4545 Fragments arranged by size: 4545 1329 927 94 SmlI C'TyrA_G Cuts at: 820 1017 1943 3536 4999 5900 6162 820 Size: 197 926 1593 1463 901 262 1553 Fragments arranged by size: 1593 1553 1463 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2616 4686 493 Size: 2123 2070 2702 Fragments arranged by size: 2702 2123 2070 SpeI A'CTAG_T Cuts at: 152 152 Size: 6895 SphI G_CATG'C Cuts at: 1132 4253 1132 Size: 3121 3774 SspI AAT'ATT Cuts at: 5 52 4819 5 Size: 47 4767 2081 Fragments arranged by size: 4767 2081 47 StuI AGG'CCT Cuts at: 1517 2441 1517 Size: 924 5971 StyI C'CwwG_G Cuts at: 513 1065 2004 2445 2565 2599 2940 3555 4167 Size: 552 939 441 120 34 341 615 612 Cuts at: 4167 4334 513 Size: 167 3074 Fragments arranged by size: 3074 939 615 612 552 441 341 167 120 34 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4922 4939 5092 5277 5722 325 Size: 1255 3342 17 153 185 445 1498 Fragments arranged by size: 3342 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3660 5141 6853 Size: 274 80 33 51 1443 1683 1481 1712 Cuts at: 6853 96 Size: 138 Fragments arranged by size: 1712 1683 1481 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2762 2794 3851 3883 6855 6887 2762 Size: 32 1057 32 2972 32 2770 Fragments arranged by size: 2972 2770 1057 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2453 3512 1316 Size: 1137 1059 4699 Fragments arranged by size: 4699 1137 1059 XbaI T'CTAG_A Cuts at: 4231 4231 Size: 6895 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3665 3665 Size: 6895 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6895 XmaI C'CCGG_G Cuts at: 1870 3199 4126 4220 1870 Size: 1329 927 94 4545 Fragments arranged by size: 4545 1329 927 94 XmnI GAAnn'nnTTC Cuts at: 2498 3045 5024 2498 Size: 547 1979 4369 Fragments arranged by size: 4369 1979 547 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4703 276 Size: 53 83 186 936 3169 2468 Fragments arranged by size: 3169 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SfiI SfoI SrfI SwaI