Restriction map for
pFN21AE1022
(Linear) (Six-base) MAPSORT of: KIEE1022.seq Check: 8368 from: 1 to: 2190
KIEE1022.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1057 1280 2190
Size: 1057 223 910
Fragments arranged by size:
1057 910 223
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 670 742 815 1168 1298 1744 1864 1948
Size: 670 72 73 353 130 446 120 84
Cuts at: 1948 2190
Size: 242
Fragments arranged by size:
670 446 353 242 130 120 84 73
72
AfeI AGC'GCT
Cuts at: 0 1093 1937 2190
Size: 1093 844 253
Fragments arranged by size:
1093 844 253
AflIII A'CryG_T
Cuts at: 0 883 2190
Size: 883 1307
AhdI GACnn_n'nnGTC
Cuts at: 0 1991 2190
Size: 1991 199
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 575 607 903 935 1054 1086 2190
Size: 575 32 296 32 119 32 1104
Fragments arranged by size:
1104 575 296 119 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 720 1082 2190
Size: 720 362 1108
Fragments arranged by size:
1108 720 362
ApaI G_GGCC'C
Cuts at: 0 2074 2190
Size: 2074 116
ApaLI G'TGCA_C
Cuts at: 0 799 2190
Size: 799 1391
AvaI C'yCGr_G
Cuts at: 0 1194 2121 2190
Size: 1194 927 69
Fragments arranged by size:
1194 927 69
BamHI G'GATC_C
Cuts at: 0 1611 2190
Size: 1611 579
BanI G'GyrC_C
Cuts at: 0 413 1097 1456 2015 2190
Size: 413 684 359 559 175
Fragments arranged by size:
684 559 413 359 175
BanII G_rGCy'C
Cuts at: 0 304 1976 2074 2190
Size: 304 1672 98 116
Fragments arranged by size:
1672 304 116 98
BbsI GAAGACnn'nnnn_
Cuts at: 0 821 1156 1286 1588 2190
Size: 821 335 130 302 602
Fragments arranged by size:
821 602 335 302 130
BbvCI CC'TCA_GC
Cuts at: 0 212 1026 1031 2190
Size: 212 814 5 1159
Fragments arranged by size:
1159 814 212 5
BclI T'GATC_A
Cuts at: 0 728 2190
Size: 728 1462
BlpI GC'TnA_GC
Cuts at: 0 298 2190
Size: 298 1892
Bme1580I G_kGCm'C
Cuts at: 0 670 803 943 1459 2074 2190
Size: 670 133 140 516 615 116
Fragments arranged by size:
670 615 516 140 133 116
BmrI ACTGGGnnnn_n'
Cuts at: 0 968 1647 2190
Size: 968 679 543
Fragments arranged by size:
968 679 543
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 453 1038 2190
Size: 453 585 1152
Fragments arranged by size:
1152 585 453
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 835 1852 1873 1915 1921 1988 2028 2190
Size: 835 1017 21 42 6 67 40 162
Fragments arranged by size:
1017 835 162 67 42 40 21 6
Bpu10I CC'TnA_GC
Cuts at: 0 212 1026 1031 2190
Size: 212 814 5 1159
Fragments arranged by size:
1159 814 212 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1516 2190
Size: 1516 674
BsaI GGTCTCn'nnnn_
Cuts at: 0 154 831 2190
Size: 154 677 1359
Fragments arranged by size:
1359 677 154
BsaAI yAC'GTr
Cuts at: 0 611 2190
Size: 611 1579
BsaHI Gr'CG_yC
Cuts at: 0 458 1555 2190
Size: 458 1097 635
Fragments arranged by size:
1097 635 458
BsaWI w'CCGG_w
Cuts at: 0 315 2018 2190
Size: 315 1703 172
Fragments arranged by size:
1703 315 172
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 575 605 1054 1084 1846 1876 2190
Size: 575 30 449 30 762 30 314
Fragments arranged by size:
762 575 449 314 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 176 397 400 445 770 1014 1726 1729
Size: 176 221 3 45 325 244 712 3
Cuts at: 1729 1849 1870 1873 2190
Size: 120 21 3 317
Fragments arranged by size:
712 325 317 244 221 176 120 45
21 3 3 3
BseYI C'CCAG_C
Cuts at: 0 841 1480 1757 2190
Size: 841 639 277 433
Fragments arranged by size:
841 639 433 277
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1330 2190
Size: 1330 860
BsiEI CG_ry'CG
Cuts at: 0 2096 2190
Size: 2096 94
BsiHKAI G_wGCw'C
Cuts at: 0 803 1070 2190
Size: 803 267 1120
Fragments arranged by size:
1120 803 267
BsmI GAATG_Cn'
Cuts at: 0 1650 2190
Size: 1650 540
BsmBI CGTCTCn'nnnn_
Cuts at: 0 31 1309 2190
Size: 31 1278 881
Fragments arranged by size:
1278 881 31
Bsp1286I G_dGCh'C
Cuts at: 0 304 670 803 943 1070 1459 1976 2074
Size: 304 366 133 140 127 389 517 98
Cuts at: 2074 2190
Size: 116
Fragments arranged by size:
517 389 366 304 140 133 127 116
98
BspEI T'CCGG_A
Cuts at: 0 315 2190
Size: 315 1875
BspHI T'CATG_A
Cuts at: 0 1415 2190
Size: 1415 775
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1057 1280 2190
Size: 1057 223 910
Fragments arranged by size:
1057 910 223
BsrBI CCG'CTC
Cuts at: 0 1173 1789 2190
Size: 1173 616 401
Fragments arranged by size:
1173 616 401
BsrDI GCAATG_nn'
Cuts at: 0 247 2190
Size: 247 1943
BsrFI r'CCGG_y
Cuts at: 0 1807 1865 2079 2190
Size: 1807 58 214 111
Fragments arranged by size:
1807 214 111 58
BssSI C'ACGA_G
Cuts at: 0 643 2190
Size: 643 1547
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2151 2190
Size: 2151 39
BstBI TT'CG_AA
Cuts at: 0 1642 2190
Size: 1642 548
BstXI CCAn_nnnn'nTGG
Cuts at: 0 447 2190
Size: 447 1743
BstYI r'GATC_y
Cuts at: 0 206 1611 2190
Size: 206 1405 579
Fragments arranged by size:
1405 579 206
BtgI C'CryG_G
Cuts at: 0 440 761 1176 1550 1616 2190
Size: 440 321 415 374 66 574
Fragments arranged by size:
574 440 415 374 321 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 364 2190
Size: 364 1826
BtsI GCAGTG_nn'
Cuts at: 0 92 721 2190
Size: 92 629 1469
Fragments arranged by size:
1469 629 92
DrdI GACnn_nn'nnGTC
Cuts at: 0 1323 2190
Size: 1323 867
EaeI y'GGCC_r
Cuts at: 0 498 1242 1964 2093 2190
Size: 498 744 722 129 97
Fragments arranged by size:
744 722 498 129 97
EagI C'GGCC_G
Cuts at: 0 2093 2190
Size: 2093 97
EarI CTCTTCn'nnn_
Cuts at: 0 374 901 1720 2101 2190
Size: 374 527 819 381 89
Fragments arranged by size:
819 527 381 374 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1894 1961 2190
Size: 1894 67 229
Fragments arranged by size:
1894 229 67
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 670 742 815 835 1168 1298 1744 1852
Size: 670 72 73 20 333 130 446 108
Cuts at: 1852 1864 1873 1915 1921 1948 1988 2028 2190
Size: 12 9 42 6 27 40 40 162
Fragments arranged by size:
670 446 333 162 130 108 73 72
42 40 40 27 20 12 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 451 2190
Size: 451 1739
EcoO109I rG'GnC_Cy
Cuts at: 0 1737 2070 2071 2190
Size: 1737 333 1 119
Fragments arranged by size:
1737 333 119 1
FspI TGC'GCA
Cuts at: 0 1237 2190
Size: 1237 953
HaeII r_GCGC'y
Cuts at: 0 1095 1939 2190
Size: 1095 844 251
Fragments arranged by size:
1095 844 251
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 969 1001 1306 1338 1963 1977 1995 2009
Size: 969 32 305 32 625 14 18 14
Cuts at: 2009 2190
Size: 181
Fragments arranged by size:
969 625 305 181 32 32 18 14
14
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1097 1487 2190
Size: 1097 390 703
Fragments arranged by size:
1097 703 390
MscI TGG'CCA
Cuts at: 0 1966 2190
Size: 1966 224
MslI CAynn'nnrTG
Cuts at: 0 1363 2190
Size: 1363 827
MspA1I CmG'CkG
Cuts at: 0 297 1030 1082 1484 1618 1851 2048 2190
Size: 297 733 52 402 134 233 197 142
Fragments arranged by size:
733 402 297 233 197 142 134 52
NaeI GCC'GGC
Cuts at: 0 1809 1867 2190
Size: 1809 58 323
Fragments arranged by size:
1809 323 58
NcoI C'CATG_G
Cuts at: 0 440 1550 2190
Size: 440 1110 640
Fragments arranged by size:
1110 640 440
NgoMIV G'CCGG_C
Cuts at: 0 1807 1865 2190
Size: 1807 58 325
Fragments arranged by size:
1807 325 58
NspI r_CATG'y
Cuts at: 0 887 2149 2190
Size: 887 1262 41
Fragments arranged by size:
1262 887 41
PasI CC'CwG_GG
Cuts at: 0 1956 2190
Size: 1956 234
PciI A'CATG_T
Cuts at: 0 883 2190
Size: 883 1307
PflMI CCAn_nnn'nTGG
Cuts at: 0 2168 2190
Size: 2168 22
PfoI T'CCnGG_A
Cuts at: 0 41 1829 2190
Size: 41 1788 361
Fragments arranged by size:
1788 361 41
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 11 43 575 607 1054 1086 2190
Size: 11 32 532 32 447 32 1104
Fragments arranged by size:
1104 532 447 32 32 32 11
PshAI GACnn'nnGTC
Cuts at: 0 1912 2190
Size: 1912 278
PspOMI G'GGCC_C
Cuts at: 0 2070 2190
Size: 2070 120
PvuII CAG'CTG
Cuts at: 0 1030 1851 2048 2190
Size: 1030 821 197 142
Fragments arranged by size:
1030 821 197 142
SacII CC_GC'GG
Cuts at: 0 1619 2190
Size: 1619 571
SapI GCTCTTCn'nnn_
Cuts at: 0 2101 2190
Size: 2101 89
SexAI A'CCwGG_T
Cuts at: 0 446 2190
Size: 446 1744
SfcI C'TryA_G
Cuts at: 0 741 1699 2190
Size: 741 958 491
Fragments arranged by size:
958 741 491
SmaI CCC'GGG
Cuts at: 0 1196 2123 2190
Size: 1196 927 67
Fragments arranged by size:
1196 927 67
SmlI C'TyrA_G
Cuts at: 0 1531 2190
Size: 1531 659
SnaBI TAC'GTA
Cuts at: 0 611 2190
Size: 611 1579
StuI AGG'CCT
Cuts at: 0 436 2190
Size: 436 1754
StyI C'CwwG_G
Cuts at: 0 440 560 594 935 1550 2162 2190
Size: 440 120 34 341 615 612 28
Fragments arranged by size:
615 612 440 341 120 34 28
TatI w'GTAC_w
Cuts at: 0 1655 2190
Size: 1655 535
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 757 789 1846 1878 2190
Size: 757 32 1057 32 312
Fragments arranged by size:
1057 757 312 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 448 1507 2190
Size: 448 1059 683
Fragments arranged by size:
1059 683 448
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1660 2190
Size: 1660 530
XmaI C'CCGG_G
Cuts at: 0 1194 2121 2190
Size: 1194 927 69
Fragments arranged by size:
1194 927 69
XmnI GAAnn'nnTTC
Cuts at: 0 493 1040 2190
Size: 493 547 1150
Fragments arranged by size:
1150 547 493
Enzymes that do cut:
AarI AcuI AfeI AflIII AhdI AloI AlwNI ApaI
ApaLI AvaI BamHI BanI BanII BbsI BbvCI BclI
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I FspI HaeII Hin4I MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PpiI PshAI PspOMI PvuII SacII SapI SexAI
SfcI SmaI SmlI SnaBI StuI StyI TatI TstI
Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AleI AlfI
ApoI AscI AseI AsiSI AvrII BaeI BbeI BcgI
BciVI BfrBI BglI BglII BmgBI BmtI BsaBI BsiWI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraI
DraIII EcoICRI EcoRI EcoRV FalI FseI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI NarI NdeI
NheI NotI NruI NsiI PacI PmeI PmlI PpuMI
PsiI PspXI PsrI PstI PvuI RsrII SacI SalI
SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SpeI
SphI SrfI SspI SwaI TaqII XbaI XhoI ZraI