(Circular) (Six-base) MAPSORT of: pFN21AE1019.seq Check: 7331 from: 1 to: 6694 pFN21AE1019 6694 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2517 4504 278 Size: 53 83 186 936 981 1987 2468 Fragments arranged by size: 2468 1987 981 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4037 1937 Size: 2100 4594 Acc65I G'GTAC_C Cuts at: 2819 4015 2819 Size: 1196 5498 AclI AA'CG_TT Cuts at: 1563 4821 5194 1563 Size: 3258 373 3063 Fragments arranged by size: 3258 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3233 3386 3680 3813 4757 6141 6409 1632 Size: 1601 153 294 133 944 1384 268 1917 Fragments arranged by size: 1917 1601 1384 944 294 268 153 133 AfeI AGC'GCT Cuts at: 2988 2988 Size: 6694 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6497 AflIII A'CryG_T Cuts at: 1203 5593 6570 1203 Size: 4390 977 1327 Fragments arranged by size: 4390 1327 977 AgeI A'CCGG_T Cuts at: 5525 5525 Size: 6694 AhdI GACnn_n'nnGTC Cuts at: 5423 5423 Size: 6694 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6694 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6694 AlwNI CAG_nnn'CTG Cuts at: 3244 4437 6009 3244 Size: 1193 1572 3929 Fragments arranged by size: 3929 1572 1193 ApaLI G'TGCA_C Cuts at: 4751 5907 4751 Size: 1156 5538 ApoI r'AATT_y Cuts at: 1493 3380 3506 4003 4229 4381 6490 1493 Size: 1887 126 497 226 152 2109 1697 Fragments arranged by size: 2109 1887 1697 497 226 152 126 AseI AT'TA_AT Cuts at: 160 1032 5248 160 Size: 872 4216 1606 Fragments arranged by size: 4216 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6694 AvaI C'yCGr_G Cuts at: 1870 1943 2113 4019 1870 Size: 73 170 1906 4545 Fragments arranged by size: 4545 1906 170 73 BamHI G'GATC_C Cuts at: 3099 4024 4391 3099 Size: 925 367 5402 Fragments arranged by size: 5402 925 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2819 4015 5470 618 Size: 325 217 627 1032 1196 1455 1842 Fragments arranged by size: 1842 1455 1196 1032 627 325 217 BanII G_rGCy'C Cuts at: 1391 2744 4013 1391 Size: 1353 1269 4072 Fragments arranged by size: 4072 1353 1269 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2238 928 Size: 971 339 5384 Fragments arranged by size: 5384 971 339 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4885 4919 1903 Size: 34 2948 34 3678 Fragments arranged by size: 3678 2948 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4586 5796 4586 Size: 1210 5484 BclI T'GATC_A Cuts at: 1548 1548 Size: 6694 BfrBI ATG'CAT Cuts at: 3476 3476 Size: 6694 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3275 5305 136 Size: 107 122 71 631 605 1603 2030 1525 Fragments arranged by size: 2030 1603 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6689 6689 Size: 6694 BlpI GC'TnA_GC Cuts at: 4111 4111 Size: 6694 Bme1580I G_kGCm'C Cuts at: 1790 4755 5911 1790 Size: 2965 1156 2573 Fragments arranged by size: 2965 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6694 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4425 5383 442 Size: 416 53 83 399 24 3008 958 1753 Fragments arranged by size: 3008 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6694 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2485 2485 Size: 6694 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2002 2406 2544 2720 3126 5354 1239 Size: 763 404 138 176 406 2228 2579 Fragments arranged by size: 2579 2228 763 406 404 176 138 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2500 3845 4819 5684 5982 2500 Size: 1345 974 865 298 3212 Fragments arranged by size: 3212 1345 974 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2473 5357 882 Size: 617 974 2884 2219 Fragments arranged by size: 2884 2219 974 617 BsaAI yAC'GTr Cuts at: 493 1204 4485 493 Size: 711 3281 2702 Fragments arranged by size: 3281 2702 711 BsaBI GATnn'nnATC Cuts at: 3945 6688 3945 Size: 2743 3951 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2514 2672 4501 4883 Size: 53 83 186 936 981 158 1829 382 Cuts at: 4883 5518 275 Size: 635 1451 Fragments arranged by size: 1829 1451 981 936 635 382 186 158 83 53 BsaWI w'CCGG_w Cuts at: 5127 5525 5799 5946 6357 5127 Size: 398 274 147 411 5464 Fragments arranged by size: 5464 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6469 Fragments arranged by size: 6469 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2681 1186 Size: 1495 5199 BseYI C'CCAG_C Cuts at: 2054 3237 3521 5897 6393 2054 Size: 1183 284 2376 496 2355 Fragments arranged by size: 2376 2355 1183 496 284 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6121 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4144 4905 5933 1540 Size: 275 186 2143 761 1028 2301 Fragments arranged by size: 2301 2143 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2744 4755 4840 5911 2744 Size: 2011 85 1071 3527 Fragments arranged by size: 3527 2011 1071 85 BsmI GAATG_Cn' Cuts at: 3027 4205 4298 3027 Size: 1178 93 5423 Fragments arranged by size: 5423 1178 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2456 2663 2456 Size: 207 6487 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2744 4013 4755 4840 5911 1391 Size: 399 954 1269 742 85 1071 2174 Fragments arranged by size: 2174 1269 1071 954 742 399 85 BspEI T'CCGG_A Cuts at: 6357 6357 Size: 6694 BspHI T'CATG_A Cuts at: 3429 4583 6313 3429 Size: 1154 1730 3810 Fragments arranged by size: 3810 1730 1154 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4049 844 Size: 3205 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4581 1394 Size: 429 2758 3507 Fragments arranged by size: 3507 2758 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3447 5189 5363 65 Size: 1183 2199 1742 174 1396 Fragments arranged by size: 2199 1742 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2354 5338 5525 1700 Size: 110 544 2984 187 2869 Fragments arranged by size: 2984 2869 544 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6694 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6694 BssSI C'ACGA_G Cuts at: 4754 5766 4754 Size: 1012 5682 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6694 BstBI TT'CG_AA Cuts at: 3764 5498 3764 Size: 1734 4960 BstEII G'GTnAC_C Cuts at: 1184 2834 1184 Size: 1650 5044 BstXI CCAn_nnnn'nTGG Cuts at: 3842 3842 Size: 6694 BstYI r'GATC_y Cuts at: 1972 2389 3099 4024 4391 4776 4793 6245 6331 Size: 417 710 925 367 385 17 1452 86 Cuts at: 6331 6343 6689 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 925 710 417 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2664 3729 2664 Size: 1065 5629 BtgI C'CryG_G Cuts at: 513 1065 3774 513 Size: 552 2709 3433 Fragments arranged by size: 3433 2709 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2787 487 Size: 683 56 1561 4394 Fragments arranged by size: 4394 1561 683 56 BtsI GCAGTG_nn' Cuts at: 3242 4210 5004 5024 3242 Size: 968 794 20 4912 Fragments arranged by size: 4912 968 794 20 ClaI AT'CG_AT Cuts at: 1554 2191 1554 Size: 637 6057 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2812 2847 529 Size: 35 2248 35 4376 Fragments arranged by size: 4376 2248 35 35 DraI TTT'AAA Cuts at: 3998 3998 Size: 6694 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2072 5701 809 Size: 507 756 3629 1802 Fragments arranged by size: 3629 1802 756 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2128 4141 5030 Size: 54 1425 50 275 18 298 2013 889 Cuts at: 5030 6659 8 Size: 1629 43 Fragments arranged by size: 2013 1629 1425 889 298 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4141 1537 Size: 275 2329 4090 Fragments arranged by size: 4090 2329 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2331 2375 2608 2640 2756 3060 3224 4624 Size: 978 44 233 32 116 304 164 1400 Cuts at: 4624 1353 Size: 3423 Fragments arranged by size: 3423 1400 978 304 233 164 116 44 32 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5281 5655 5801 387 Size: 1064 138 3692 374 146 1280 Fragments arranged by size: 3692 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2742 2742 Size: 6694 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2002 2406 2544 2720 3126 3233 3386 Size: 393 370 404 138 176 406 107 153 Cuts at: 3386 3680 3813 4757 5354 6141 6409 1239 Size: 294 133 944 597 787 268 1524 Fragments arranged by size: 1524 944 787 597 406 404 393 370 294 268 176 153 138 133 107 EcoO109I rG'GnC_Cy Cuts at: 3370 3370 Size: 6694 EcoRI G'AATT_C Cuts at: 4003 4381 4003 Size: 378 6316 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2360 2360 Size: 6694 FspI TGC'GCA Cuts at: 5200 5200 Size: 6694 HaeII r_GCGC'y Cuts at: 1678 2990 5841 1678 Size: 1312 2851 2531 Fragments arranged by size: 2851 2531 1312 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2207 2239 2612 2644 5335 Size: 32 135 32 635 32 373 32 2691 Cuts at: 5335 5367 5409 5441 1373 Size: 32 42 32 2626 Fragments arranged by size: 2691 2626 635 373 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4038 4284 669 Size: 988 281 2100 246 3079 Fragments arranged by size: 3079 2100 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6694 HpaI GTT'AAC Cuts at: 4284 4284 Size: 6694 KpnI G_GTAC'C Cuts at: 2823 4019 2823 Size: 1196 5498 MfeI C'AATT_G Cuts at: 4293 4293 Size: 6694 MluI A'CGCG_T Cuts at: 6570 6570 Size: 6694 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3317 5808 5992 1305 Size: 2012 2491 184 2007 Fragments arranged by size: 2491 2012 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2130 10 Size: 54 1425 343 298 4574 Fragments arranged by size: 4574 1425 343 298 54 MslI CAynn'nnrTG Cuts at: 518 1166 3932 4652 5011 5170 518 Size: 648 2766 720 359 159 2042 Fragments arranged by size: 2766 2042 720 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2058 3521 4110 4787 5935 6180 1606 Size: 452 1463 589 677 1148 245 2120 Fragments arranged by size: 2120 1463 1148 677 589 452 245 NcoI C'CATG_G Cuts at: 513 1065 3774 513 Size: 552 2709 3433 Fragments arranged by size: 3433 2709 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6694 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6694 NotI GC'GGCC_GC Cuts at: 4141 4141 Size: 6694 NsiI A_TGCA'T Cuts at: 3478 3478 Size: 6694 NspI r_CATG'y Cuts at: 1132 1229 4052 5597 1132 Size: 97 2823 1545 2229 Fragments arranged by size: 2823 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2026 1348 Size: 678 6016 PciI A'CATG_T Cuts at: 5593 5593 Size: 6694 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2032 1108 Size: 902 22 5770 Fragments arranged by size: 5770 902 22 PmeI GTTT'AAAC Cuts at: 3998 3998 Size: 6694 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4763 4795 6276 6308 4763 Size: 32 1481 32 5149 Fragments arranged by size: 5149 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6694 PsiI TTA'TAA Cuts at: 3977 4264 3977 Size: 287 6407 PspXI vC'TCGA_Gb Cuts at: 2113 2113 Size: 6694 PstI C_TGCA'G Cuts at: 830 4046 830 Size: 3216 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6694 PvuII CAG'CTG Cuts at: 3521 3521 Size: 6694 SacI G_AGCT'C Cuts at: 2744 2744 Size: 6694 SalI G'TCGA_C Cuts at: 1936 4036 1936 Size: 2100 4594 SapI GCTCTTCn'nnn_ Cuts at: 2756 3224 2756 Size: 468 6226 SbfI CC_TGCA'GG Cuts at: 4046 4046 Size: 6694 ScaI AGT'ACT Cuts at: 4942 6654 4942 Size: 1712 4982 SfcI C'TryA_G Cuts at: 826 1045 3311 4042 5177 5858 6049 826 Size: 219 2266 731 1135 681 191 1471 Fragments arranged by size: 2266 1471 1135 731 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6694 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6694 SmaI CCC'GGG Cuts at: 1872 4021 1872 Size: 2149 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2113 2479 3860 4798 5699 5961 Size: 197 926 170 366 1381 938 901 262 Cuts at: 5961 820 Size: 1553 Fragments arranged by size: 1553 1381 938 926 901 366 262 197 170 SnaBI TAC'GTA Cuts at: 493 4485 493 Size: 3992 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6694 SphI G_CATG'C Cuts at: 1132 4052 1132 Size: 2920 3774 SspI AAT'ATT Cuts at: 5 52 3901 4618 5 Size: 47 3849 717 2081 Fragments arranged by size: 3849 2081 717 47 StuI AGG'CCT Cuts at: 1517 3090 1517 Size: 1573 5121 StyI C'CwwG_G Cuts at: 513 1065 2608 2745 3774 4133 513 Size: 552 1543 137 1029 359 3074 Fragments arranged by size: 3074 1543 1029 552 359 137 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3693 4721 4738 4891 5076 5521 325 Size: 1255 2113 1028 17 153 185 445 1498 Fragments arranged by size: 2113 1498 1255 1028 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2133 3707 3814 Size: 274 80 33 51 1443 156 1574 107 Cuts at: 3814 4940 6652 96 Size: 1126 1712 138 Fragments arranged by size: 1712 1574 1443 1126 274 156 138 107 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2324 2356 6654 6686 2324 Size: 32 4298 32 2332 Fragments arranged by size: 4298 2332 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6694 XbaI T'CTAG_A Cuts at: 4030 4030 Size: 6694 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2167 3966 2167 Size: 1799 4895 XhoI C'TCGA_G Cuts at: 1943 2113 1943 Size: 170 6524 XmaI C'CCGG_G Cuts at: 1870 4019 1870 Size: 2149 4545 XmnI GAAnn'nnTTC Cuts at: 3218 4823 3218 Size: 1605 5089 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2515 4502 276 Size: 53 83 186 936 981 1987 2468 Fragments arranged by size: 2468 1987 981 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI