(Circular) (Six-base) MAPSORT of: pFN21AE1018.seq Check: 3388 from: 1 to: 6622 pFN21AE1018 6622 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4432 278 Size: 53 83 186 936 2896 2468 Fragments arranged by size: 2896 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2671 3965 1937 Size: 734 1294 4594 Fragments arranged by size: 4594 1294 734 Acc65I G'GTAC_C Cuts at: 3943 3943 Size: 6622 AclI AA'CG_TT Cuts at: 1563 4749 5122 1563 Size: 3186 373 3063 Fragments arranged by size: 3186 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2716 2884 3556 4685 6069 6337 1632 Size: 1084 168 672 1129 1384 268 1917 Fragments arranged by size: 1917 1384 1129 1084 672 268 168 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6425 AflIII A'CryG_T Cuts at: 1203 3919 5521 6498 1203 Size: 2716 1602 977 1327 Fragments arranged by size: 2716 1602 1327 977 AgeI A'CCGG_T Cuts at: 5453 5453 Size: 6622 AhdI GACnn_n'nnGTC Cuts at: 5351 5351 Size: 6622 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6622 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6622 AlwNI CAG_nnn'CTG Cuts at: 2018 2134 4365 5937 2018 Size: 116 2231 1572 2703 Fragments arranged by size: 2703 2231 1572 116 ApaLI G'TGCA_C Cuts at: 4679 5835 4679 Size: 1156 5466 ApoI r'AATT_y Cuts at: 1493 3606 3931 4157 4309 6418 1493 Size: 2113 325 226 152 2109 1697 Fragments arranged by size: 2113 2109 1697 325 226 152 AseI AT'TA_AT Cuts at: 160 1032 5176 160 Size: 872 4144 1606 Fragments arranged by size: 4144 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6622 AvaI C'yCGr_G Cuts at: 1870 1943 3947 1870 Size: 73 2004 4545 Fragments arranged by size: 4545 2004 73 BamHI G'GATC_C Cuts at: 3952 4319 3952 Size: 367 6255 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3943 5398 618 Size: 325 217 627 2156 1455 1842 Fragments arranged by size: 2156 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3941 1391 Size: 2550 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5651 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4813 4847 1903 Size: 34 2876 34 3678 Fragments arranged by size: 3678 2876 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4514 5724 4514 Size: 1210 5412 BclI T'GATC_A Cuts at: 1548 1548 Size: 6622 BfrBI ATG'CAT Cuts at: 3716 3716 Size: 6622 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5233 136 Size: 107 122 71 631 605 3561 1525 Fragments arranged by size: 3561 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6617 6617 Size: 6622 BlpI GC'TnA_GC Cuts at: 4039 4039 Size: 6622 Bme1580I G_kGCm'C Cuts at: 1790 4683 5839 1790 Size: 2893 1156 2573 Fragments arranged by size: 2893 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2167 1318 Size: 849 5773 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4353 5311 442 Size: 416 53 83 399 24 2936 958 1753 Fragments arranged by size: 2936 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6622 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2195 3048 3804 5282 1239 Size: 956 853 756 1478 2579 Fragments arranged by size: 2579 1478 956 853 756 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2219 4747 5612 5910 2219 Size: 2528 865 298 2931 Fragments arranged by size: 2931 2528 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2171 2860 5285 882 Size: 617 672 689 2425 2219 Fragments arranged by size: 2425 2219 689 672 617 BsaAI yAC'GTr Cuts at: 493 1204 4413 493 Size: 711 3209 2702 Fragments arranged by size: 3209 2702 711 BsaBI GATnn'nnATC Cuts at: 6616 6616 Size: 6622 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4429 4811 5446 275 Size: 53 83 186 936 2896 382 635 1451 Fragments arranged by size: 2896 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5055 5453 5727 5874 6285 5055 Size: 398 274 147 411 5392 Fragments arranged by size: 5392 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2114 2144 1178 Size: 30 165 30 711 30 5656 Fragments arranged by size: 5656 711 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2135 1186 Size: 949 5673 BseYI C'CCAG_C Cuts at: 5825 6321 5825 Size: 496 6126 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6049 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4072 4833 5861 1540 Size: 275 186 2071 761 1028 2301 Fragments arranged by size: 2301 2071 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2407 4683 4768 5839 2407 Size: 2276 85 1071 3190 Fragments arranged by size: 3190 2276 1071 85 BsmI GAATG_Cn' Cuts at: 2258 3050 4133 4226 2258 Size: 792 1083 93 4654 Fragments arranged by size: 4654 1083 792 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2157 2157 Size: 6622 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2407 3941 4683 4768 5839 1391 Size: 399 617 1534 742 85 1071 2174 Fragments arranged by size: 2174 1534 1071 742 617 399 85 BspEI T'CCGG_A Cuts at: 6285 6285 Size: 6622 BspHI T'CATG_A Cuts at: 2416 4511 6241 2416 Size: 2095 1730 2797 Fragments arranged by size: 2797 2095 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2200 3977 844 Size: 1356 1777 3489 Fragments arranged by size: 3489 1777 1356 BsrBI CCG'CTC Cuts at: 1394 1823 4509 1394 Size: 429 2686 3507 Fragments arranged by size: 3507 2686 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3475 5117 5291 65 Size: 1183 2227 1642 174 1396 Fragments arranged by size: 2227 1642 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5266 5453 1700 Size: 110 3456 187 2869 Fragments arranged by size: 3456 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2157 2755 3343 3763 3803 3847 96 Size: 2061 598 588 420 40 44 2871 Fragments arranged by size: 2871 2061 598 588 420 44 40 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6622 BssSI C'ACGA_G Cuts at: 2406 4682 5694 2406 Size: 2276 1012 3334 Fragments arranged by size: 3334 2276 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6622 BstBI TT'CG_AA Cuts at: 5426 5426 Size: 6622 BstEII G'GTnAC_C Cuts at: 1184 2212 1184 Size: 1028 5594 BstYI r'GATC_y Cuts at: 1972 3952 4319 4704 4721 6173 6259 6271 6617 Size: 1980 367 385 17 1452 86 12 346 Cuts at: 6617 1972 Size: 1977 Fragments arranged by size: 1980 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3880 513 Size: 552 2815 3255 Fragments arranged by size: 3255 2815 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5883 Fragments arranged by size: 5883 683 56 BtsI GCAGTG_nn' Cuts at: 4138 4932 4952 4138 Size: 794 20 5808 Fragments arranged by size: 5808 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6622 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6587 DraI TTT'AAA Cuts at: 3926 3926 Size: 6622 DraIII CAC_nnn'GTG Cuts at: 3726 3726 Size: 6622 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5629 809 Size: 507 4313 1802 Fragments arranged by size: 4313 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4069 4958 6587 Size: 54 1425 50 275 18 2239 889 1629 Cuts at: 6587 8 Size: 43 Fragments arranged by size: 2239 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4069 1537 Size: 275 2257 4090 Fragments arranged by size: 4090 2257 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3880 4552 1353 Size: 2527 672 3423 Fragments arranged by size: 3423 2527 672 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5209 5583 5729 387 Size: 1064 138 3620 374 146 1280 Fragments arranged by size: 3620 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2195 2716 2884 3048 3556 3804 4685 Size: 393 563 521 168 164 508 248 881 Cuts at: 4685 5282 6069 6337 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 881 787 597 563 521 508 393 268 248 168 164 EcoO109I rG'GnC_Cy Cuts at: 2151 2151 Size: 6622 EcoRI G'AATT_C Cuts at: 3606 3931 4309 3606 Size: 325 378 5919 Fragments arranged by size: 5919 378 325 FspI TGC'GCA Cuts at: 5128 5128 Size: 6622 HaeII r_GCGC'y Cuts at: 1678 5769 1678 Size: 4091 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2314 2346 5263 5295 5337 Size: 32 135 32 742 32 2917 32 42 Cuts at: 5337 5369 1373 Size: 32 2626 Fragments arranged by size: 2917 2626 742 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2913 3165 3249 3753 3966 4212 Size: 988 281 975 252 84 504 213 246 Cuts at: 4212 669 Size: 3079 Fragments arranged by size: 3079 988 975 504 281 252 246 213 84 HindIII A'AGCT_T Cuts at: 748 2041 2811 3077 3651 748 Size: 1293 770 266 574 3719 Fragments arranged by size: 3719 1293 770 574 266 HpaI GTT'AAC Cuts at: 4212 4212 Size: 6622 KpnI G_GTAC'C Cuts at: 3947 3947 Size: 6622 MfeI C'AATT_G Cuts at: 4221 4221 Size: 6622 MluI A'CGCG_T Cuts at: 6498 6498 Size: 6622 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2220 2347 3021 3626 5736 5920 1305 Size: 915 127 674 605 2110 184 2007 Fragments arranged by size: 2110 2007 915 674 605 184 127 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4800 Fragments arranged by size: 4800 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2334 4580 4939 5098 518 Size: 648 1168 2246 359 159 2042 Fragments arranged by size: 2246 2042 1168 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4038 4715 5863 6108 1606 Size: 2432 677 1148 245 2120 Fragments arranged by size: 2432 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 3880 513 Size: 552 2815 3255 Fragments arranged by size: 3255 2815 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6622 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6622 NotI GC'GGCC_GC Cuts at: 4069 4069 Size: 6622 NsiI A_TGCA'T Cuts at: 3718 3718 Size: 6622 NspI r_CATG'y Cuts at: 1132 1229 2721 3720 3923 3980 5525 1132 Size: 97 1492 999 203 57 1545 2229 Fragments arranged by size: 2229 1545 1492 999 203 97 57 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6622 PciI A'CATG_T Cuts at: 3919 5521 3919 Size: 1602 5020 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6622 PfoI T'CCnGG_A Cuts at: 2605 2605 Size: 6622 PmeI GTTT'AAAC Cuts at: 3926 3926 Size: 6622 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4691 4723 6204 6236 4691 Size: 32 1481 32 5077 Fragments arranged by size: 5077 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2151 2151 Size: 6622 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6622 PsiI TTA'TAA Cuts at: 4192 4192 Size: 6622 PstI C_TGCA'G Cuts at: 830 2542 3974 830 Size: 1712 1432 3478 Fragments arranged by size: 3478 1712 1432 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6622 SalI G'TCGA_C Cuts at: 1936 3964 1936 Size: 2028 4594 SbfI CC_TGCA'GG Cuts at: 3974 3974 Size: 6622 ScaI AGT'ACT Cuts at: 4870 6582 4870 Size: 1712 4910 SfcI C'TryA_G Cuts at: 826 1045 2182 2538 3970 5105 5786 5977 826 Size: 219 1137 356 1432 1135 681 191 1471 Fragments arranged by size: 1471 1432 1137 1135 681 356 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6622 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6622 SmaI CCC'GGG Cuts at: 1872 3949 1872 Size: 2077 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2234 4726 5627 5889 820 Size: 197 926 291 2492 901 262 1553 Fragments arranged by size: 2492 1553 926 901 291 262 197 SnaBI TAC'GTA Cuts at: 493 4413 493 Size: 3920 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6622 SphI G_CATG'C Cuts at: 1132 3720 3980 1132 Size: 2588 260 3774 Fragments arranged by size: 3774 2588 260 SspI AAT'ATT Cuts at: 5 52 4546 5 Size: 47 4494 2081 Fragments arranged by size: 4494 2081 47 StuI AGG'CCT Cuts at: 1517 2664 1517 Size: 1147 5475 StyI C'CwwG_G Cuts at: 513 1065 3337 3880 4061 513 Size: 552 2272 543 181 3074 Fragments arranged by size: 3074 2272 552 543 181 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2129 4649 4666 4819 5004 5449 325 Size: 1255 549 2520 17 153 185 445 1498 Fragments arranged by size: 2520 1498 1255 549 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2157 2444 2711 Size: 274 80 33 51 1443 180 287 267 Cuts at: 2711 2755 3215 3343 3763 3803 3847 4868 6580 Size: 44 460 128 420 40 44 1021 1712 Cuts at: 6580 96 Size: 138 Fragments arranged by size: 1712 1443 1021 460 420 287 274 267 180 138 128 80 51 44 44 40 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6582 6614 6582 Size: 32 6590 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6622 XbaI T'CTAG_A Cuts at: 3958 3958 Size: 6622 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6622 XmaI C'CCGG_G Cuts at: 1870 3947 1870 Size: 2077 4545 XmnI GAAnn'nnTTC Cuts at: 2729 3149 3233 3358 3401 4751 2729 Size: 420 84 125 43 1350 4600 Fragments arranged by size: 4600 1350 420 125 84 43 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4430 276 Size: 53 83 186 936 2896 2468 Fragments arranged by size: 2896 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI