(Circular) (Six-base) MAPSORT of: pFN21AB9259.seq Check: 8147 from: 1 to: 7642 pFN21AB9259 7642 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3100 3907 3976 3100 Size: 807 69 6766 Fragments arranged by size: 6766 807 69 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5452 278 Size: 53 83 186 936 3916 2468 Fragments arranged by size: 3916 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4985 1937 Size: 3048 4594 Acc65I G'GTAC_C Cuts at: 4963 4963 Size: 7642 AclI AA'CG_TT Cuts at: 1563 5769 6142 1563 Size: 4206 373 3063 Fragments arranged by size: 4206 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2202 3598 5705 7089 7357 1632 Size: 570 1396 2107 1384 268 1917 Fragments arranged by size: 2107 1917 1396 1384 570 268 AfeI AGC'GCT Cuts at: 4022 4796 4022 Size: 774 6868 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7445 AflIII A'CryG_T Cuts at: 1203 6541 7518 1203 Size: 5338 977 1327 Fragments arranged by size: 5338 1327 977 AgeI A'CCGG_T Cuts at: 3143 6473 3143 Size: 3330 4312 AhdI GACnn_n'nnGTC Cuts at: 2981 6371 2981 Size: 3390 4252 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7642 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4390 1184 Size: 3206 4436 AlwNI CAG_nnn'CTG Cuts at: 3415 4618 5385 6957 3415 Size: 1203 767 1572 4100 Fragments arranged by size: 4100 1572 1203 767 ApaI G_GGCC'C Cuts at: 2084 2098 2190 2297 2454 2598 3728 4164 4171 Size: 14 92 107 157 144 1130 436 7 Cuts at: 4171 2084 Size: 5555 Fragments arranged by size: 5555 1130 436 157 144 107 92 14 7 ApaLI G'TGCA_C Cuts at: 5699 6855 5699 Size: 1156 6486 ApoI r'AATT_y Cuts at: 1493 4951 5177 5329 7438 1493 Size: 3458 226 152 2109 1697 Fragments arranged by size: 3458 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6196 160 Size: 872 5164 1606 Fragments arranged by size: 5164 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7642 AvaI C'yCGr_G Cuts at: 1870 1943 2152 2453 2644 2917 3037 3810 4164 Size: 73 209 301 191 273 120 773 354 Cuts at: 4164 4481 4922 4967 1870 Size: 317 441 45 4545 Fragments arranged by size: 4545 773 441 354 317 301 273 209 191 120 73 45 BamHI G'GATC_C Cuts at: 3336 4582 4972 5339 3336 Size: 1246 390 367 5639 Fragments arranged by size: 5639 1246 390 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2342 2517 2569 2760 3112 Size: 325 217 627 555 175 52 191 352 Cuts at: 3112 3796 3802 4440 4963 6418 618 Size: 684 6 638 523 1455 1842 Fragments arranged by size: 1842 1455 684 638 627 555 523 352 325 217 191 175 52 6 BanII G_rGCy'C Cuts at: 1391 2084 2098 2114 2139 2190 2297 2454 2490 Size: 693 14 16 25 51 107 157 36 Cuts at: 2490 2504 2598 2651 3063 3353 3728 4152 4164 Size: 14 94 53 412 290 375 424 12 Cuts at: 4164 4171 4961 1391 Size: 7 790 4072 Fragments arranged by size: 4072 790 693 424 412 375 290 157 107 94 53 51 36 25 16 14 14 12 7 BbeI G_GCGC'C Cuts at: 2573 3800 2573 Size: 1227 6415 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2319 4107 4177 928 Size: 971 420 1788 70 4393 Fragments arranged by size: 4393 1788 971 420 70 BbvCI CC'TCA_GC Cuts at: 2604 2604 Size: 7642 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5833 5867 1903 Size: 34 3896 34 3678 Fragments arranged by size: 3896 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3026 5534 6744 3026 Size: 2508 1210 3924 Fragments arranged by size: 3924 2508 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7642 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3527 4077 4091 Size: 107 122 71 631 605 1855 550 14 Cuts at: 4091 6253 136 Size: 2162 1525 Fragments arranged by size: 2162 1855 1525 631 605 550 122 107 71 14 BglII A'GATC_T Cuts at: 4911 7637 4911 Size: 2726 4916 BlpI GC'TnA_GC Cuts at: 3392 5059 3392 Size: 1667 5975 Bme1580I G_kGCm'C Cuts at: 1790 2084 2098 2152 2190 2297 2347 2454 2477 Size: 294 14 54 38 107 50 107 23 Cuts at: 2477 2598 3728 4164 4171 4605 5703 6859 1790 Size: 121 1130 436 7 434 1098 1156 2573 Fragments arranged by size: 2573 1156 1130 1098 436 434 294 121 107 107 54 50 38 23 14 7 BmgBI CAC'GTC Cuts at: 1318 2077 1318 Size: 759 6883 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3136 5373 6331 Size: 416 53 83 399 24 1719 2237 958 Cuts at: 6331 442 Size: 1753 Fragments arranged by size: 2237 1753 1719 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7642 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2143 3946 3988 2143 Size: 1803 42 5797 Fragments arranged by size: 5797 1803 42 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2098 2585 2643 2673 2891 3277 3437 3470 Size: 859 487 58 30 218 386 160 33 Cuts at: 3470 3809 4079 4085 4451 4514 4697 4736 6302 Size: 339 270 6 366 63 183 39 1566 Cuts at: 6302 1239 Size: 2579 Fragments arranged by size: 2579 1566 859 487 386 366 339 270 218 183 160 63 58 39 33 30 6 Bpu10I CC'TnA_GC Cuts at: 2192 2604 2192 Size: 412 7230 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2920 3525 4652 4676 5767 6632 6930 2920 Size: 605 1127 24 1091 865 298 3632 Fragments arranged by size: 3632 1127 1091 865 605 298 24 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3068 6305 882 Size: 617 1569 3237 2219 Fragments arranged by size: 3237 2219 1569 617 BsaAI yAC'GTr Cuts at: 493 1204 4343 5433 493 Size: 711 3139 1090 2702 Fragments arranged by size: 3139 2702 1090 711 BsaBI GATnn'nnATC Cuts at: 4916 7636 4916 Size: 2720 4922 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2570 3468 3797 5449 Size: 53 83 186 936 1037 898 329 1652 Cuts at: 5449 5831 6466 275 Size: 382 635 1451 Fragments arranged by size: 1652 1451 1037 936 898 635 382 329 186 83 53 BsaWI w'CCGG_w Cuts at: 3070 3143 6075 6473 6747 6894 7305 3070 Size: 73 2932 398 274 147 411 3407 Fragments arranged by size: 3407 2932 411 398 274 147 73 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3410 3437 3440 3467 4332 Size: 30 165 30 2007 27 3 27 865 Cuts at: 4332 4362 4424 4454 4515 4545 1178 Size: 30 62 30 61 30 4275 Fragments arranged by size: 4275 2007 865 165 62 61 30 30 30 30 30 27 27 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2640 2740 3230 3280 3554 3592 3878 4499 Size: 1454 100 490 50 274 38 286 621 Cuts at: 4499 4742 1186 Size: 243 4086 Fragments arranged by size: 4086 1454 621 490 286 274 243 100 50 38 BseYI C'CCAG_C Cuts at: 2044 2107 4152 6845 7341 2044 Size: 63 2045 2693 496 2345 Fragments arranged by size: 2693 2345 2045 496 63 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2555 2932 3935 4004 4067 1163 Size: 573 819 377 1003 69 63 4738 Fragments arranged by size: 4738 1003 819 573 377 69 63 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2026 3720 3902 4312 5092 5853 Size: 275 186 25 1694 182 410 780 761 Cuts at: 5853 6881 1540 Size: 1028 2301 Fragments arranged by size: 2301 1694 1028 780 761 410 275 186 182 25 BsiHKAI G_wGCw'C Cuts at: 3353 4437 5703 5788 6859 3353 Size: 1084 1266 85 1071 4136 Fragments arranged by size: 4136 1266 1084 1071 85 BsiWI C'GTAC_G Cuts at: 4242 4242 Size: 7642 BsmI GAATG_Cn' Cuts at: 5153 5246 5153 Size: 93 7549 BsmBI CGTCTCn'nnnn_ Cuts at: 3917 3917 Size: 7642 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2084 2098 2114 2139 2152 2190 2297 Size: 399 294 14 16 25 13 38 107 Cuts at: 2297 2347 2454 2477 2490 2504 2598 2651 3063 Size: 50 107 23 13 14 94 53 412 Cuts at: 3063 3353 3728 4152 4164 4171 4437 4605 4961 Size: 290 375 424 12 7 266 168 356 Cuts at: 4961 5703 5788 6859 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 424 412 399 375 356 294 290 266 168 107 107 94 85 53 50 38 25 23 16 14 14 13 13 12 7 BspEI T'CCGG_A Cuts at: 3070 7305 3070 Size: 4235 3407 BspHI T'CATG_A Cuts at: 5531 7261 5531 Size: 1730 5912 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2112 3100 3486 3907 3976 4997 844 Size: 1268 988 386 421 69 1021 3489 Fragments arranged by size: 3489 1268 1021 988 421 386 69 BsrBI CCG'CTC Cuts at: 1394 1823 3186 3222 5529 1394 Size: 429 1363 36 2307 3507 Fragments arranged by size: 3507 2307 1363 429 36 BsrDI GCAATG_nn' Cuts at: 65 1248 6137 6311 65 Size: 1183 4889 174 1396 Fragments arranged by size: 4889 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3143 3530 3799 4252 4307 4783 4815 Size: 110 1333 387 269 453 55 476 32 Cuts at: 4815 6286 6473 1700 Size: 1471 187 2869 Fragments arranged by size: 2869 1471 1333 476 453 387 269 187 110 55 32 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7642 BssHII G'CGCG_C Cuts at: 1925 3779 4832 4858 1925 Size: 1854 1053 26 4709 Fragments arranged by size: 4709 1854 1053 26 BssSI C'ACGA_G Cuts at: 3701 5702 6714 3701 Size: 2001 1012 4629 Fragments arranged by size: 4629 2001 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7642 BstBI TT'CG_AA Cuts at: 6446 6446 Size: 7642 BstEII G'GTnAC_C Cuts at: 1184 2012 3134 1184 Size: 828 1122 5692 Fragments arranged by size: 5692 1122 828 BstXI CCAn_nnnn'nTGG Cuts at: 2968 3977 2968 Size: 1009 6633 BstYI r'GATC_y Cuts at: 1972 3162 3336 4488 4500 4582 4911 4972 5339 Size: 1190 174 1152 12 82 329 61 367 Cuts at: 5339 5724 5741 7193 7279 7291 7637 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1190 1152 385 367 346 329 174 86 82 61 17 12 12 Bsu36I CC'TnA_GG Cuts at: 2223 4722 2223 Size: 2499 5143 BtgI C'CryG_G Cuts at: 513 1065 2017 3204 3462 3771 4086 4809 513 Size: 552 952 1187 258 309 315 723 3346 Fragments arranged by size: 3346 1187 952 723 552 315 309 258 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3411 487 Size: 683 56 2185 4718 Fragments arranged by size: 4718 2185 683 56 BtsI GCAGTG_nn' Cuts at: 2475 2500 4235 4513 4587 5158 5952 5972 2475 Size: 25 1735 278 74 571 794 20 4145 Fragments arranged by size: 4145 1735 794 571 278 74 25 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7642 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7607 DraI TTT'AAA Cuts at: 4946 4946 Size: 7642 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6649 809 Size: 507 5333 1802 Fragments arranged by size: 5333 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2287 3717 3899 Size: 54 1425 50 275 18 457 1430 182 Cuts at: 3899 4083 4092 4254 4309 4851 4884 5089 5978 Size: 184 9 162 55 542 33 205 889 Cuts at: 5978 7607 8 Size: 1629 43 Fragments arranged by size: 1629 1430 1425 889 542 457 275 205 184 182 162 55 54 50 43 33 18 9 EagI C'GGCC_G Cuts at: 1537 1812 3717 3899 4309 5089 1537 Size: 275 1905 182 410 780 4090 Fragments arranged by size: 4090 1905 780 410 275 182 EarI CTCTTCn'nnn_ Cuts at: 1353 2605 3987 5572 1353 Size: 1252 1382 1585 3423 Fragments arranged by size: 3423 1585 1382 1252 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3736 4478 4734 4862 6229 6603 Size: 1064 138 2147 742 256 128 1367 374 Cuts at: 6603 6749 387 Size: 146 1280 Fragments arranged by size: 2147 1367 1280 1064 742 374 256 146 138 128 EcoICRI GAG'CTC Cuts at: 3351 3351 Size: 7642 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2098 2202 2585 2643 2673 2891 3277 Size: 393 466 104 383 58 30 218 386 Cuts at: 3277 3437 3470 3598 3809 4079 4085 4451 4514 Size: 160 33 128 211 270 6 366 63 Cuts at: 4514 4697 4736 5705 6302 7089 7357 1239 Size: 183 39 969 597 787 268 1524 Fragments arranged by size: 1524 969 787 597 466 393 386 383 366 270 268 218 211 183 160 128 104 63 58 39 33 30 6 EcoNI CCTnn'n_nnAGG Cuts at: 2201 2226 2272 3172 4132 2201 Size: 25 46 900 960 5711 Fragments arranged by size: 5711 960 900 46 25 EcoO109I rG'GnC_Cy Cuts at: 2081 2094 2095 2186 2187 2219 2293 2294 2450 Size: 13 1 91 1 32 74 1 156 Cuts at: 2450 2657 2838 2886 3622 3652 3724 3725 4069 Size: 207 181 48 736 30 72 1 344 Cuts at: 4069 4108 4161 4168 4615 4633 4738 2081 Size: 39 53 7 447 18 105 4985 Fragments arranged by size: 4985 736 447 344 207 181 156 105 91 74 72 53 48 39 32 30 18 13 7 1 1 1 1 EcoRI G'AATT_C Cuts at: 4951 5329 4951 Size: 378 7264 FseI GG_CCGG'CC Cuts at: 4311 4311 Size: 7642 FspI TGC'GCA Cuts at: 6148 6148 Size: 7642 HaeII r_GCGC'y Cuts at: 1678 2465 2573 2917 3739 3800 4024 4798 4899 Size: 787 108 344 822 61 224 774 101 Cuts at: 4899 6789 1678 Size: 1890 2531 Fragments arranged by size: 2531 1890 822 787 774 344 224 108 101 61 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2195 2227 2963 2967 2995 Size: 32 135 32 623 32 736 4 28 Cuts at: 2995 2999 3437 3469 6283 6315 6357 6389 1373 Size: 4 438 32 2814 32 42 32 2626 Fragments arranged by size: 2814 2626 736 623 438 135 42 32 32 32 32 32 32 28 4 4 HincII GTy'rAC Cuts at: 669 1657 1938 4986 5232 669 Size: 988 281 3048 246 3079 Fragments arranged by size: 3079 3048 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7642 HpaI GTT'AAC Cuts at: 5232 5232 Size: 7642 KasI G'GCGC_C Cuts at: 2569 3796 2569 Size: 1227 6415 KpnI G_GTAC'C Cuts at: 4967 4967 Size: 7642 MfeI C'AATT_G Cuts at: 5241 5241 Size: 7642 MluI A'CGCG_T Cuts at: 7518 7518 Size: 7642 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2907 6756 6940 1305 Size: 1602 3849 184 2007 Fragments arranged by size: 3849 2007 1602 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2289 4085 4094 10 Size: 54 1425 343 457 1796 9 3558 Fragments arranged by size: 3558 1796 1425 457 343 54 9 MslI CAynn'nnrTG Cuts at: 518 1166 2954 2960 3911 4403 5600 5959 6118 Size: 648 1788 6 951 492 1197 359 159 Cuts at: 6118 518 Size: 2042 Fragments arranged by size: 2042 1788 1197 951 648 492 359 159 6 MspA1I CmG'CkG Cuts at: 1606 2237 2937 3415 3581 3889 4704 4822 5058 Size: 631 700 478 166 308 815 118 236 Cuts at: 5058 5735 6883 7128 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 815 700 677 631 478 308 245 236 166 118 NaeI GCC'GGC Cuts at: 3532 4254 4309 3532 Size: 722 55 6865 Fragments arranged by size: 6865 722 55 NarI GG'CG_CC Cuts at: 2570 3797 2570 Size: 1227 6415 NcoI C'CATG_G Cuts at: 513 1065 2017 3204 3771 513 Size: 552 952 1187 567 4384 Fragments arranged by size: 4384 1187 952 567 552 NdeI CA'TA_TG Cuts at: 387 2070 387 Size: 1683 5959 NgoMIV G'CCGG_C Cuts at: 3530 4252 4307 3530 Size: 722 55 6865 Fragments arranged by size: 6865 722 55 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7642 NotI GC'GGCC_GC Cuts at: 5089 5089 Size: 7642 NspI r_CATG'y Cuts at: 1132 1229 2959 3499 5000 6545 1132 Size: 97 1730 540 1501 1545 2229 Fragments arranged by size: 2229 1730 1545 1501 540 97 PasI CC'CwG_GG Cuts at: 1348 2843 4301 1348 Size: 1495 1458 4689 Fragments arranged by size: 4689 1495 1458 PciI A'CATG_T Cuts at: 6541 6541 Size: 7642 PflMI CCAn_nnn'nTGG Cuts at: 1108 2563 2781 3415 3463 4450 4618 4704 1108 Size: 1455 218 634 48 987 168 86 4046 Fragments arranged by size: 4046 1455 987 634 218 168 86 48 PfoI T'CCnGG_A Cuts at: 2367 4322 4491 4611 4674 4713 2367 Size: 1955 169 120 63 39 5296 Fragments arranged by size: 5296 1955 169 120 63 39 PmeI GTTT'AAAC Cuts at: 4946 4946 Size: 7642 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5711 5743 7224 7256 5711 Size: 32 1481 32 6097 Fragments arranged by size: 6097 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2886 4615 4633 4738 2886 Size: 1729 18 105 5790 Fragments arranged by size: 5790 1729 105 18 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7642 PsiI TTA'TAA Cuts at: 5212 5212 Size: 7642 PspOMI G'GGCC_C Cuts at: 2080 2094 2186 2293 2450 2594 3724 4160 4167 Size: 14 92 107 157 144 1130 436 7 Cuts at: 4167 2080 Size: 5555 Fragments arranged by size: 5555 1130 436 157 144 107 92 14 7 PstI C_TGCA'G Cuts at: 830 3111 3743 4116 4994 830 Size: 2281 632 373 878 3478 Fragments arranged by size: 3478 2281 878 632 373 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7642 PvuII CAG'CTG Cuts at: 3415 4704 3415 Size: 1289 6353 SacI G_AGCT'C Cuts at: 3353 3353 Size: 7642 SalI G'TCGA_C Cuts at: 1936 4984 1936 Size: 3048 4594 SbfI CC_TGCA'GG Cuts at: 4994 4994 Size: 7642 ScaI AGT'ACT Cuts at: 5890 7602 5890 Size: 1712 5930 SexAI A'CCwGG_T Cuts at: 2520 2520 Size: 7642 SfcI C'TryA_G Cuts at: 826 1045 3107 3364 3739 4112 4640 4682 4990 Size: 219 2062 257 375 373 528 42 308 Cuts at: 4990 6125 6806 6997 826 Size: 1135 681 191 1471 Fragments arranged by size: 2062 1471 1135 681 528 375 373 308 257 219 191 42 SfiI GGCCn_nnn'nGGCC Cuts at: 3527 4077 4091 3527 Size: 550 14 7078 Fragments arranged by size: 7078 550 14 SfoI GGC'GCC Cuts at: 2571 3798 2571 Size: 1227 6415 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7642 SgrAI Cr'CCGG_yG Cuts at: 1700 3143 3799 1700 Size: 1443 656 5543 Fragments arranged by size: 5543 1443 656 SmaI CCC'GGG Cuts at: 1872 2455 2646 3039 3812 4166 4969 1872 Size: 583 191 393 773 354 803 4545 Fragments arranged by size: 4545 803 773 583 393 354 191 SmlI C'TyrA_G Cuts at: 820 1017 1943 2899 3540 4667 4691 5746 6647 Size: 197 926 956 641 1127 24 1055 901 Cuts at: 6647 6909 820 Size: 262 1553 Fragments arranged by size: 1553 1127 1055 956 926 901 641 262 197 24 SnaBI TAC'GTA Cuts at: 493 5433 493 Size: 4940 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7642 SphI G_CATG'C Cuts at: 1132 3499 5000 1132 Size: 2367 1501 3774 Fragments arranged by size: 3774 2367 1501 SrfI GCCC'GGGC Cuts at: 2455 2646 2455 Size: 191 7451 SspI AAT'ATT Cuts at: 5 52 5566 5 Size: 47 5514 2081 Fragments arranged by size: 5514 2081 47 StuI AGG'CCT Cuts at: 1517 2123 4080 1517 Size: 606 1957 5079 Fragments arranged by size: 5079 1957 606 StyI C'CwwG_G Cuts at: 513 1065 2017 2598 3078 3204 3771 5081 513 Size: 552 952 581 480 126 567 1310 3074 Fragments arranged by size: 3074 1310 952 581 567 552 480 126 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2040 2343 2971 3764 5669 5686 5839 Size: 1255 460 303 628 793 1905 17 153 Cuts at: 5839 6024 6469 325 Size: 185 445 1498 Fragments arranged by size: 1905 1498 1255 793 628 460 445 303 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5888 7600 96 Size: 274 80 33 51 1443 3911 1712 138 Fragments arranged by size: 3911 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2693 2725 3321 3353 3410 3442 4332 4364 4424 Size: 32 596 32 57 32 890 32 60 Cuts at: 4424 4456 4515 4547 7602 7634 2693 Size: 32 59 32 3055 32 2701 Fragments arranged by size: 3055 2701 890 596 60 59 57 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7642 XbaI T'CTAG_A Cuts at: 4978 4978 Size: 7642 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3026 3026 Size: 7642 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7642 XmaI C'CCGG_G Cuts at: 1870 2453 2644 3037 3810 4164 4967 1870 Size: 583 191 393 773 354 803 4545 Fragments arranged by size: 4545 803 773 583 393 354 191 XmnI GAAnn'nnTTC Cuts at: 5771 5771 Size: 7642 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5450 276 Size: 53 83 186 936 3916 2468 Fragments arranged by size: 3916 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BstZ17I DraIII EcoRV FalI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SwaI