Restriction map for
pFN21AB9259
(Linear) (Six-base) MAPSORT of: KIBB9259.seq Check: 3269 from: 1 to: 2937
KIBB9259.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1095 1902 1971 2937
Size: 1095 807 69 966
Fragments arranged by size:
1095 966 807 69
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 197 1593 2937
Size: 197 1396 1344
Fragments arranged by size:
1396 1344 197
AfeI AGC'GCT
Cuts at: 0 2017 2791 2937
Size: 2017 774 146
Fragments arranged by size:
2017 774 146
AgeI A'CCGG_T
Cuts at: 0 1138 2937
Size: 1138 1799
AhdI GACnn_n'nnGTC
Cuts at: 0 976 2937
Size: 976 1961
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2385 2937
Size: 2385 552
AlwNI CAG_nnn'CTG
Cuts at: 0 1410 2613 2937
Size: 1410 1203 324
Fragments arranged by size:
1410 1203 324
ApaI G_GGCC'C
Cuts at: 0 79 93 185 292 449 593 1723 2159
Size: 79 14 92 107 157 144 1130 436
Cuts at: 2159 2166 2937
Size: 7 771
Fragments arranged by size:
1130 771 436 157 144 107 92 79
14 7
AvaI C'yCGr_G
Cuts at: 0 147 448 639 912 1032 1805 2159 2476
Size: 147 301 191 273 120 773 354 317
Cuts at: 2476 2917 2937
Size: 441 20
Fragments arranged by size:
773 441 354 317 301 273 191 147
120 20
BamHI G'GATC_C
Cuts at: 0 1331 2577 2937
Size: 1331 1246 360
Fragments arranged by size:
1331 1246 360
BanI G'GyrC_C
Cuts at: 0 337 512 564 755 1107 1791 1797 2435
Size: 337 175 52 191 352 684 6 638
Cuts at: 2435 2937
Size: 502
Fragments arranged by size:
684 638 502 352 337 191 175 52
6
BanII G_rGCy'C
Cuts at: 0 79 93 109 134 185 292 449 485
Size: 79 14 16 25 51 107 157 36
Cuts at: 485 499 593 646 1058 1348 1723 2147 2159
Size: 14 94 53 412 290 375 424 12
Cuts at: 2159 2166 2937
Size: 7 771
Fragments arranged by size:
771 424 412 375 290 157 107 94
79 53 51 36 25 16 14 14
12 7
BbeI G_GCGC'C
Cuts at: 0 568 1795 2937
Size: 568 1227 1142
Fragments arranged by size:
1227 1142 568
BbsI GAAGACnn'nnnn_
Cuts at: 0 314 2102 2172 2937
Size: 314 1788 70 765
Fragments arranged by size:
1788 765 314 70
BbvCI CC'TCA_GC
Cuts at: 0 599 2937
Size: 599 2338
BciVI GTATCCnnnnn_n'
Cuts at: 0 1021 2937
Size: 1021 1916
BglI GCCn_nnn'nGGC
Cuts at: 0 1522 2072 2086 2937
Size: 1522 550 14 851
Fragments arranged by size:
1522 851 550 14
BglII A'GATC_T
Cuts at: 0 2906 2937
Size: 2906 31
BlpI GC'TnA_GC
Cuts at: 0 1387 2937
Size: 1387 1550
Bme1580I G_kGCm'C
Cuts at: 0 79 93 147 185 292 342 449 472
Size: 79 14 54 38 107 50 107 23
Cuts at: 472 593 1723 2159 2166 2600 2937
Size: 121 1130 436 7 434 337
Fragments arranged by size:
1130 436 434 337 121 107 107 79
54 50 38 23 14 7
BmgBI CAC'GTC
Cuts at: 0 72 2937
Size: 72 2865
BmrI ACTGGGnnnn_n'
Cuts at: 0 1131 2937
Size: 1131 1806
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 138 1941 1983 2937
Size: 138 1803 42 954
Fragments arranged by size:
1803 954 138 42
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 580 638 668 886 1272 1432 1465
Size: 93 487 58 30 218 386 160 33
Cuts at: 1465 1804 2074 2080 2446 2509 2692 2731 2937
Size: 339 270 6 366 63 183 39 206
Fragments arranged by size:
487 386 366 339 270 218 206 183
160 93 63 58 39 33 30 6
Bpu10I CC'TnA_GC
Cuts at: 0 187 599 2937
Size: 187 412 2338
Fragments arranged by size:
2338 412 187
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 915 1520 2647 2671 2937
Size: 915 605 1127 24 266
Fragments arranged by size:
1127 915 605 266 24
BsaI GGTCTCn'nnnn_
Cuts at: 0 1063 2937
Size: 1063 1874
BsaAI yAC'GTr
Cuts at: 0 2338 2937
Size: 2338 599
BsaBI GATnn'nnATC
Cuts at: 0 2911 2937
Size: 2911 26
BsaHI Gr'CG_yC
Cuts at: 0 565 1463 1792 2937
Size: 565 898 329 1145
Fragments arranged by size:
1145 898 565 329
BsaWI w'CCGG_w
Cuts at: 0 1065 1138 2937
Size: 1065 73 1799
Fragments arranged by size:
1799 1065 73
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1405 1432 1435 1462 2327 2357 2419 2449
Size: 1405 27 3 27 865 30 62 30
Cuts at: 2449 2510 2540 2937
Size: 61 30 397
Fragments arranged by size:
1405 865 397 62 61 30 30 30
27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 635 735 1225 1275 1549 1587 1873 2494
Size: 635 100 490 50 274 38 286 621
Cuts at: 2494 2737 2937
Size: 243 200
Fragments arranged by size:
635 621 490 286 274 243 200 100
50 38
BseYI C'CCAG_C
Cuts at: 0 39 102 2147 2937
Size: 39 63 2045 790
Fragments arranged by size:
2045 790 63 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 927 1930 1999 2062 2937
Size: 550 377 1003 69 63 875
Fragments arranged by size:
1003 875 550 377 69 63
BsiEI CG_ry'CG
Cuts at: 0 21 1715 1897 2307 2937
Size: 21 1694 182 410 630
Fragments arranged by size:
1694 630 410 182 21
BsiHKAI G_wGCw'C
Cuts at: 0 1348 2432 2937
Size: 1348 1084 505
Fragments arranged by size:
1348 1084 505
BsiWI C'GTAC_G
Cuts at: 0 2237 2937
Size: 2237 700
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1912 2937
Size: 1912 1025
Bsp1286I G_dGCh'C
Cuts at: 0 79 93 109 134 147 185 292 342
Size: 79 14 16 25 13 38 107 50
Cuts at: 342 449 472 485 499 593 646 1058 1348
Size: 107 23 13 14 94 53 412 290
Cuts at: 1348 1723 2147 2159 2166 2432 2600 2937
Size: 375 424 12 7 266 168 337
Fragments arranged by size:
424 412 375 337 290 266 168 107
107 94 79 53 50 38 25 23
16 14 14 13 13 12 7
BspEI T'CCGG_A
Cuts at: 0 1065 2937
Size: 1065 1872
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 107 1095 1481 1902 1971 2937
Size: 107 988 386 421 69 966
Fragments arranged by size:
988 966 421 386 107 69
BsrBI CCG'CTC
Cuts at: 0 1181 1217 2937
Size: 1181 36 1720
Fragments arranged by size:
1720 1181 36
BsrFI r'CCGG_y
Cuts at: 0 1138 1525 1794 2247 2302 2778 2810 2937
Size: 1138 387 269 453 55 476 32 127
Fragments arranged by size:
1138 476 453 387 269 127 55 32
BssHII G'CGCG_C
Cuts at: 0 1774 2827 2853 2937
Size: 1774 1053 26 84
Fragments arranged by size:
1774 1053 84 26
BssSI C'ACGA_G
Cuts at: 0 1696 2937
Size: 1696 1241
BstEII G'GTnAC_C
Cuts at: 0 7 1129 2937
Size: 7 1122 1808
Fragments arranged by size:
1808 1122 7
BstXI CCAn_nnnn'nTGG
Cuts at: 0 963 1972 2937
Size: 963 1009 965
Fragments arranged by size:
1009 965 963
BstYI r'GATC_y
Cuts at: 0 1157 1331 2483 2495 2577 2906 2937
Size: 1157 174 1152 12 82 329 31
Fragments arranged by size:
1157 1152 329 174 82 31 12
Bsu36I CC'TnA_GG
Cuts at: 0 218 2717 2937
Size: 218 2499 220
Fragments arranged by size:
2499 220 218
BtgI C'CryG_G
Cuts at: 0 12 1199 1457 1766 2081 2804 2937
Size: 12 1187 258 309 315 723 133
Fragments arranged by size:
1187 723 315 309 258 133 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1406 2937
Size: 1406 1531
BtsI GCAGTG_nn'
Cuts at: 0 470 495 2230 2508 2582 2937
Size: 470 25 1735 278 74 355
Fragments arranged by size:
1735 470 355 278 74 25
EaeI y'GGCC_r
Cuts at: 0 282 1712 1894 2078 2087 2249 2304 2846
Size: 282 1430 182 184 9 162 55 542
Cuts at: 2846 2879 2937
Size: 33 58
Fragments arranged by size:
1430 542 282 184 182 162 58 55
33 9
EagI C'GGCC_G
Cuts at: 0 1712 1894 2304 2937
Size: 1712 182 410 633
Fragments arranged by size:
1712 633 410 182
EarI CTCTTCn'nnn_
Cuts at: 0 600 1982 2937
Size: 600 1382 955
Fragments arranged by size:
1382 955 600
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1731 2473 2729 2857 2937
Size: 1731 742 256 128 80
Fragments arranged by size:
1731 742 256 128 80
EcoICRI GAG'CTC
Cuts at: 0 1346 2937
Size: 1346 1591
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 197 580 638 668 886 1272 1432
Size: 93 104 383 58 30 218 386 160
Cuts at: 1432 1465 1593 1804 2074 2080 2446 2509 2692
Size: 33 128 211 270 6 366 63 183
Cuts at: 2692 2731 2937
Size: 39 206
Fragments arranged by size:
386 383 366 270 218 211 206 183
160 128 104 93 63 58 39 33
30 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 196 221 267 1167 2127 2937
Size: 196 25 46 900 960 810
Fragments arranged by size:
960 900 810 196 46 25
EcoO109I rG'GnC_Cy
Cuts at: 0 76 89 90 181 182 214 288 289
Size: 76 13 1 91 1 32 74 1
Cuts at: 289 445 652 833 881 1617 1647 1719 1720
Size: 156 207 181 48 736 30 72 1
Cuts at: 1720 2064 2103 2156 2163 2610 2628 2733 2937
Size: 344 39 53 7 447 18 105 204
Fragments arranged by size:
736 447 344 207 204 181 156 105
91 76 74 72 53 48 39 32
30 18 13 7 1 1 1 1
FseI GG_CCGG'CC
Cuts at: 0 2306 2937
Size: 2306 631
HaeII r_GCGC'y
Cuts at: 0 460 568 912 1734 1795 2019 2793 2894
Size: 460 108 344 822 61 224 774 101
Cuts at: 2894 2937
Size: 43
Fragments arranged by size:
822 774 460 344 224 108 101 61
43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 190 222 958 962 990 994 1432 1464
Size: 190 32 736 4 28 4 438 32
Cuts at: 1464 2937
Size: 1473
Fragments arranged by size:
1473 736 438 190 32 32 28 4
4
KasI G'GCGC_C
Cuts at: 0 564 1791 2937
Size: 564 1227 1146
Fragments arranged by size:
1227 1146 564
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 902 2937
Size: 902 2035
MscI TGG'CCA
Cuts at: 0 284 2080 2089 2937
Size: 284 1796 9 848
Fragments arranged by size:
1796 848 284 9
MslI CAynn'nnrTG
Cuts at: 0 949 955 1906 2398 2937
Size: 949 6 951 492 539
Fragments arranged by size:
951 949 539 492 6
MspA1I CmG'CkG
Cuts at: 0 232 932 1410 1576 1884 2699 2817 2937
Size: 232 700 478 166 308 815 118 120
Fragments arranged by size:
815 700 478 308 232 166 120 118
NaeI GCC'GGC
Cuts at: 0 1527 2249 2304 2937
Size: 1527 722 55 633
Fragments arranged by size:
1527 722 633 55
NarI GG'CG_CC
Cuts at: 0 565 1792 2937
Size: 565 1227 1145
Fragments arranged by size:
1227 1145 565
NcoI C'CATG_G
Cuts at: 0 12 1199 1766 2937
Size: 12 1187 567 1171
Fragments arranged by size:
1187 1171 567 12
NdeI CA'TA_TG
Cuts at: 0 65 2937
Size: 65 2872
NgoMIV G'CCGG_C
Cuts at: 0 1525 2247 2302 2937
Size: 1525 722 55 635
Fragments arranged by size:
1525 722 635 55
NspI r_CATG'y
Cuts at: 0 954 1494 2937
Size: 954 540 1443
Fragments arranged by size:
1443 954 540
PasI CC'CwG_GG
Cuts at: 0 838 2296 2937
Size: 838 1458 641
Fragments arranged by size:
1458 838 641
PflMI CCAn_nnn'nTGG
Cuts at: 0 558 776 1410 1458 2445 2613 2699 2937
Size: 558 218 634 48 987 168 86 238
Fragments arranged by size:
987 634 558 238 218 168 86 48
PfoI T'CCnGG_A
Cuts at: 0 362 2317 2486 2606 2669 2708 2937
Size: 362 1955 169 120 63 39 229
Fragments arranged by size:
1955 362 229 169 120 63 39
PpuMI rG'GwC_Cy
Cuts at: 0 881 2610 2628 2733 2937
Size: 881 1729 18 105 204
Fragments arranged by size:
1729 881 204 105 18
PspOMI G'GGCC_C
Cuts at: 0 75 89 181 288 445 589 1719 2155
Size: 75 14 92 107 157 144 1130 436
Cuts at: 2155 2162 2937
Size: 7 775
Fragments arranged by size:
1130 775 436 157 144 107 92 75
14 7
PstI C_TGCA'G
Cuts at: 0 1106 1738 2111 2937
Size: 1106 632 373 826
Fragments arranged by size:
1106 826 632 373
PvuII CAG'CTG
Cuts at: 0 1410 2699 2937
Size: 1410 1289 238
Fragments arranged by size:
1410 1289 238
SacI G_AGCT'C
Cuts at: 0 1348 2937
Size: 1348 1589
SexAI A'CCwGG_T
Cuts at: 0 515 2937
Size: 515 2422
SfcI C'TryA_G
Cuts at: 0 1102 1359 1734 2107 2635 2677 2937
Size: 1102 257 375 373 528 42 260
Fragments arranged by size:
1102 528 375 373 260 257 42
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1522 2072 2086 2937
Size: 1522 550 14 851
Fragments arranged by size:
1522 851 550 14
SfoI GGC'GCC
Cuts at: 0 566 1793 2937
Size: 566 1227 1144
Fragments arranged by size:
1227 1144 566
SgrAI Cr'CCGG_yG
Cuts at: 0 1138 1794 2937
Size: 1138 656 1143
Fragments arranged by size:
1143 1138 656
SmaI CCC'GGG
Cuts at: 0 450 641 1034 1807 2161 2937
Size: 450 191 393 773 354 776
Fragments arranged by size:
776 773 450 393 354 191
SmlI C'TyrA_G
Cuts at: 0 894 1535 2662 2686 2937
Size: 894 641 1127 24 251
Fragments arranged by size:
1127 894 641 251 24
SphI G_CATG'C
Cuts at: 0 1494 2937
Size: 1494 1443
SrfI GCCC'GGGC
Cuts at: 0 450 641 2937
Size: 450 191 2296
Fragments arranged by size:
2296 450 191
StuI AGG'CCT
Cuts at: 0 118 2075 2937
Size: 118 1957 862
Fragments arranged by size:
1957 862 118
StyI C'CwwG_G
Cuts at: 0 12 593 1073 1199 1766 2937
Size: 12 581 480 126 567 1171
Fragments arranged by size:
1171 581 567 480 126 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 35 338 966 1759 2937
Size: 35 303 628 793 1178
Fragments arranged by size:
1178 793 628 303 35
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 688 720 1316 1348 1405 1437 2327 2359
Size: 688 32 596 32 57 32 890 32
Cuts at: 2359 2419 2451 2510 2542 2937
Size: 60 32 59 32 395
Fragments arranged by size:
890 688 596 395 60 59 57 32
32 32 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1021 2937
Size: 1021 1916
XmaI C'CCGG_G
Cuts at: 0 448 639 1032 1805 2159 2937
Size: 448 191 393 773 354 778
Fragments arranged by size:
778 773 448 393 354 191
Enzymes that do cut:
AarI AcuI AfeI AgeI AhdI AlfI AlwNI ApaI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrFI BssHII BssSI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PasI PflMI PfoI PpuMI PspOMI
PstI PvuII SacI SexAI SfcI SfiI SfoI SgrAI
SmaI SmlI SphI SrfI StuI StyI TaqII TstI
XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AleI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BcgI
BclI BfrBI BmtI BsmI BspHI BsrDI BsrGI BstAPI
BstBI BstZ17I ClaI CspCI DraI DraIII DrdI EcoRI
EcoRV FalI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI NheI NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SgfI
SnaBI SpeI SspI SwaI TatI Tth111I XbaI XhoI
XmnI ZraI