(Circular) (Six-base) MAPSORT of: pFN21AB9127.seq Check: 898 from: 1 to: 7162 pFN21AB9127 7162 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4972 278 Size: 53 83 186 936 3436 2468 Fragments arranged by size: 3436 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4505 1937 Size: 2568 4594 Acc65I G'GTAC_C Cuts at: 4483 4483 Size: 7162 AclI AA'CG_TT Cuts at: 1563 3869 4205 5289 5662 1563 Size: 2306 336 1084 373 3063 Fragments arranged by size: 3063 2306 1084 373 336 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5225 6609 6877 1632 Size: 3593 1384 268 1917 Fragments arranged by size: 3593 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6965 AflIII A'CryG_T Cuts at: 1203 6061 7038 1203 Size: 4858 977 1327 Fragments arranged by size: 4858 1327 977 AgeI A'CCGG_T Cuts at: 5993 5993 Size: 7162 AhdI GACnn_n'nnGTC Cuts at: 5891 5891 Size: 7162 AleI CACnn'nnGTG Cuts at: 1166 2907 2967 3075 3807 4227 1166 Size: 1741 60 108 732 420 4101 Fragments arranged by size: 4101 1741 732 420 108 60 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7162 AlwNI CAG_nnn'CTG Cuts at: 3102 4905 6477 3102 Size: 1803 1572 3787 Fragments arranged by size: 3787 1803 1572 ApaLI G'TGCA_C Cuts at: 3810 4062 4230 5219 6375 3810 Size: 252 168 989 1156 4597 Fragments arranged by size: 4597 1156 989 252 168 ApoI r'AATT_y Cuts at: 1493 2011 2269 2324 2391 2420 2638 2664 2814 Size: 518 258 55 67 29 218 26 150 Cuts at: 2814 2898 3066 3150 3234 3318 3402 3570 3654 Size: 84 168 84 84 84 84 168 84 Cuts at: 3654 3738 3822 3906 4158 4242 4300 4326 4471 Size: 84 84 84 252 84 58 26 145 Cuts at: 4471 4697 4849 6958 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 518 258 252 226 218 168 168 152 150 145 84 84 84 84 84 84 84 84 84 84 67 58 55 29 26 26 AseI AT'TA_AT Cuts at: 160 1032 2184 5716 160 Size: 872 1152 3532 1606 Fragments arranged by size: 3532 1606 1152 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7162 AvaI C'yCGr_G Cuts at: 1870 1943 4487 1870 Size: 73 2544 4545 Fragments arranged by size: 4545 2544 73 BamHI G'GATC_C Cuts at: 4492 4859 4492 Size: 367 6795 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4483 5938 618 Size: 325 217 627 2696 1455 1842 Fragments arranged by size: 2696 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2285 4481 1391 Size: 894 2196 4072 Fragments arranged by size: 4072 2196 894 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2792 3551 3956 928 Size: 971 893 759 405 4134 Fragments arranged by size: 4134 971 893 759 405 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5353 5387 1903 Size: 34 3416 34 3678 Fragments arranged by size: 3678 3416 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5054 6264 5054 Size: 1210 5952 BclI T'GATC_A Cuts at: 1548 1548 Size: 7162 BfrBI ATG'CAT Cuts at: 4417 4417 Size: 7162 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3893 5773 136 Size: 107 122 71 631 605 2221 1880 1525 Fragments arranged by size: 2221 1880 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2708 3278 7157 2708 Size: 570 3879 2713 Fragments arranged by size: 3879 2713 570 BlpI GC'TnA_GC Cuts at: 2284 4579 2284 Size: 2295 4867 Bme1580I G_kGCm'C Cuts at: 1790 3814 3898 4066 4234 5223 6379 1790 Size: 2024 84 168 168 989 1156 2573 Fragments arranged by size: 2573 2024 1156 989 168 168 84 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7162 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3840 4893 5851 Size: 416 53 83 399 24 2423 1053 958 Cuts at: 5851 442 Size: 1753 Fragments arranged by size: 2423 1753 1053 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7162 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2337 5822 1239 Size: 1098 3485 2579 Fragments arranged by size: 3485 2579 1098 Bpu10I CC'TnA_GC Cuts at: 3997 3997 Size: 7162 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3203 5287 6152 6450 3203 Size: 2084 865 298 3915 Fragments arranged by size: 3915 2084 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5825 882 Size: 617 4326 2219 Fragments arranged by size: 4326 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4953 493 Size: 711 3749 2702 Fragments arranged by size: 3749 2702 711 BsaBI GATnn'nnATC Cuts at: 7156 7156 Size: 7162 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4969 5351 5986 275 Size: 53 83 186 936 3436 382 635 1451 Fragments arranged by size: 3436 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5595 5993 6267 6414 6825 5595 Size: 398 274 147 411 5932 Fragments arranged by size: 5932 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6937 Fragments arranged by size: 6937 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7162 BseYI C'CCAG_C Cuts at: 6365 6861 6365 Size: 496 6666 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6589 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4612 5373 6401 1540 Size: 275 186 2611 761 1028 2301 Fragments arranged by size: 2611 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2285 2890 3058 3142 3310 3478 3814 3982 4066 Size: 605 168 84 168 168 336 168 84 Cuts at: 4066 4234 5223 5308 6379 2285 Size: 168 989 85 1071 3068 Fragments arranged by size: 3068 1071 989 605 336 168 168 168 168 168 85 84 84 BsmI GAATG_Cn' Cuts at: 3098 4022 4283 4419 4673 4766 3098 Size: 924 261 136 254 93 5494 Fragments arranged by size: 5494 924 261 254 136 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2285 2890 3058 3142 3310 3478 3814 Size: 399 495 605 168 84 168 168 336 Cuts at: 3814 3898 3982 4066 4234 4481 5223 5308 6379 Size: 84 84 84 168 247 742 85 1071 Cuts at: 6379 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 605 495 399 336 247 168 168 168 168 85 84 84 84 84 BspEI T'CCGG_A Cuts at: 6825 6825 Size: 7162 BspHI T'CATG_A Cuts at: 5051 6781 5051 Size: 1730 5432 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4517 844 Size: 3673 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5049 1394 Size: 429 3226 3507 Fragments arranged by size: 3507 3226 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5657 5831 65 Size: 1183 4409 174 1396 Fragments arranged by size: 4409 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5806 5993 1700 Size: 110 3996 187 2869 Fragments arranged by size: 3996 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2345 2971 96 Size: 2249 626 4287 Fragments arranged by size: 4287 2249 626 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7162 BssSI C'ACGA_G Cuts at: 5222 6234 5222 Size: 1012 6150 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7162 BstBI TT'CG_AA Cuts at: 5966 5966 Size: 7162 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7162 BstYI r'GATC_y Cuts at: 1972 2708 3278 4492 4859 5244 5261 6713 6799 Size: 736 570 1214 367 385 17 1452 86 Cuts at: 6799 6811 7157 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1214 736 570 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3890 3890 Size: 7162 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6610 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6423 Fragments arranged by size: 6423 683 56 BtsI GCAGTG_nn' Cuts at: 2779 3103 4027 4678 5472 5492 2779 Size: 324 924 651 794 20 4449 Fragments arranged by size: 4449 924 794 651 324 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7162 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2107 2142 2683 2718 529 Size: 35 1543 35 541 35 4973 Fragments arranged by size: 4973 1543 541 35 35 35 DraI TTT'AAA Cuts at: 4466 4466 Size: 7162 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6169 809 Size: 507 4853 1802 Fragments arranged by size: 4853 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4609 5498 7127 Size: 54 1425 50 275 18 2779 889 1629 Cuts at: 7127 8 Size: 43 Fragments arranged by size: 2779 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4609 1537 Size: 275 2797 4090 Fragments arranged by size: 4090 2797 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5092 1353 Size: 3739 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5749 6123 6269 387 Size: 1064 138 4160 374 146 1280 Fragments arranged by size: 4160 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2283 2283 Size: 7162 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2337 5225 5822 6609 6877 1239 Size: 393 705 2888 597 787 268 1524 Fragments arranged by size: 2888 1524 787 705 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2496 2496 Size: 7162 EcoRI G'AATT_C Cuts at: 2391 2638 2664 2814 2898 3066 3150 3234 3318 Size: 247 26 150 84 168 84 84 84 Cuts at: 3318 3402 3570 3654 3738 3822 3906 4158 4242 Size: 84 168 84 84 84 84 252 84 Cuts at: 4242 4326 4471 4849 2391 Size: 84 145 378 4704 Fragments arranged by size: 4704 378 252 247 168 168 150 145 84 84 84 84 84 84 84 84 84 84 84 26 FspI TGC'GCA Cuts at: 5668 5668 Size: 7162 HaeII r_GCGC'y Cuts at: 1678 6309 1678 Size: 4631 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2779 2811 2863 2895 3043 Size: 32 135 32 1207 32 52 32 148 Cuts at: 3043 3075 3295 3327 3955 3987 5803 5835 5877 Size: 32 220 32 628 32 1816 32 42 Cuts at: 5877 5909 1373 Size: 32 2626 Fragments arranged by size: 2626 1816 1207 628 220 148 135 52 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4506 4752 669 Size: 988 281 2568 246 3079 Fragments arranged by size: 3079 2568 988 281 246 HindIII A'AGCT_T Cuts at: 748 2625 4287 748 Size: 1877 1662 3623 Fragments arranged by size: 3623 1877 1662 HpaI GTT'AAC Cuts at: 4752 4752 Size: 7162 KpnI G_GTAC'C Cuts at: 4487 4487 Size: 7162 MfeI C'AATT_G Cuts at: 4761 4761 Size: 7162 MluI A'CGCG_T Cuts at: 7038 7038 Size: 7162 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6276 6460 1305 Size: 4971 184 2007 Fragments arranged by size: 4971 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5340 Fragments arranged by size: 5340 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2150 2907 2967 3075 3807 4227 4412 Size: 648 984 757 60 108 732 420 185 Cuts at: 4412 5120 5479 5638 518 Size: 708 359 159 2042 Fragments arranged by size: 2042 984 757 732 708 648 420 359 185 159 108 60 MspA1I CmG'CkG Cuts at: 1606 2123 4578 5255 6403 6648 1606 Size: 517 2455 677 1148 245 2120 Fragments arranged by size: 2455 2120 1148 677 517 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6610 NdeI CA'TA_TG Cuts at: 387 4054 387 Size: 3667 3495 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7162 NotI GC'GGCC_GC Cuts at: 4609 4609 Size: 7162 NsiI A_TGCA'T Cuts at: 4419 4419 Size: 7162 NspI r_CATG'y Cuts at: 1132 1229 4443 4520 6065 1132 Size: 97 3214 77 1545 2229 Fragments arranged by size: 3214 2229 1545 97 77 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7162 PciI A'CATG_T Cuts at: 6061 6061 Size: 7162 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7162 PfoI T'CCnGG_A Cuts at: 2575 2575 Size: 7162 PmeI GTTT'AAAC Cuts at: 4466 4466 Size: 7162 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5231 5263 6744 6776 5231 Size: 32 1481 32 5617 Fragments arranged by size: 5617 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7162 PsiI TTA'TAA Cuts at: 4732 4732 Size: 7162 PstI C_TGCA'G Cuts at: 830 4514 830 Size: 3684 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7162 SacI G_AGCT'C Cuts at: 2285 2285 Size: 7162 SalI G'TCGA_C Cuts at: 1936 4504 1936 Size: 2568 4594 SbfI CC_TGCA'GG Cuts at: 4514 4514 Size: 7162 ScaI AGT'ACT Cuts at: 5410 7122 5410 Size: 1712 5450 SfcI C'TryA_G Cuts at: 826 1045 4510 5645 6326 6517 826 Size: 219 3465 1135 681 191 1471 Fragments arranged by size: 3465 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7162 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7162 SmaI CCC'GGG Cuts at: 1872 4489 1872 Size: 2617 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3218 5266 6167 6429 820 Size: 197 926 1275 2048 901 262 1553 Fragments arranged by size: 2048 1553 1275 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4953 493 Size: 4460 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7162 SphI G_CATG'C Cuts at: 1132 4520 1132 Size: 3388 3774 SspI AAT'ATT Cuts at: 5 52 5086 5 Size: 47 5034 2081 Fragments arranged by size: 5034 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7162 StyI C'CwwG_G Cuts at: 513 1065 4601 513 Size: 552 3536 3074 Fragments arranged by size: 3536 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5189 5206 5359 5544 5989 325 Size: 1255 3609 17 153 185 445 1498 Fragments arranged by size: 3609 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2296 2345 2971 Size: 274 80 33 51 1443 319 49 626 Cuts at: 2971 3557 4147 4315 5408 7120 96 Size: 586 590 168 1093 1712 138 Fragments arranged by size: 1712 1443 1093 626 590 586 319 274 168 138 80 51 49 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2092 2124 2571 2603 7122 7154 2092 Size: 32 447 32 4519 32 2100 Fragments arranged by size: 4519 2100 447 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7162 XbaI T'CTAG_A Cuts at: 4498 4498 Size: 7162 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7162 XmaI C'CCGG_G Cuts at: 1870 4487 1870 Size: 2617 4545 XmnI GAAnn'nnTTC Cuts at: 2627 2861 2945 3617 3785 3953 4037 4205 5291 Size: 234 84 672 168 168 84 168 1086 Cuts at: 5291 2627 Size: 4498 Fragments arranged by size: 4498 1086 672 234 168 168 168 84 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4970 276 Size: 53 83 186 936 3436 2468 Fragments arranged by size: 3436 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI