Restriction map for
pFN21AB6014
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# NO CUT ENZYMES ( 90 enzymes) #
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AatII Acc65I AccI AclI AflII
AgeI AocI Aor51HI AscI AseI
AsnI Asp718I AviII AvrII BanI
BbeI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsiWI BsmBI BsmI BspDI
BspHI BspLU11I Bst1107I BstAPI BstBI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DrdI Eco47III Eco81I EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
FspI HpaI KasI KpnI MamI
MfeI MluI MunI NarI NdeI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccIII 1596 TCCGGA T^CCGGA
AcyI 181 GACGCC G[GA]^CG[CT]C
AflIII 1706 ACACGT A^C[GA][CT]GT
AhdI 976 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1838 GTGCAC G^TGCAC
AlwNI 1900 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1838 GTGCAC G^TGCAC
Asp700I 1299 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 976 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1186 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1657 CACGTG CAC^GTG
BsaHI 181 GACGCC G[GA]^CG[CT]C
BsaI 1805 GAGACC GGTCTC(1/5)
BseAI 1596 TCCGGA T^CCGGA
BsgI 250 GTGCAG GTGCAG(16/14)
Bsp1407I 965 TGTACA T^GTACA
BspEI 1596 TCCGGA T^CCGGA
BspMI 51 ACCTGC ACCTGC(4/8)
BsrBI 44 GAGCGG CCGCTC(-3/-3)
BsrDI 863 CATTGC GCAATG(2/0)
BsrFI 548 GCCGGC [GA]^CCGG[CT]
BsrGI 965 TGTACA T^GTACA
BssHII 25 GCGCGC G^CGCGC
BssSI 247 CTCGTG CACGAG(-5/-1)
Cfr10I 548 GCCGGC [GA]^CCGG[CT]
DraIII 1832 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 976 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeII 68 GGCGCT [GA]GCGC^[CT]
Hin1I 181 GACGCC G[GA]^CG[CT]C
HindIII 1579 AAGCTT A^AGCTT
MroI 1596 TCCGGA T^CCGGA
NaeI 548 GCCGGC GCC^GGC
NgoMIV 548 GCCGGC G^CCGGC
NheI 117 GCTAGC G^CTAGC
PflFI 1186 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1657 CACGTG CAC^GTG
PmlI 1657 CACGTG CAC^GTG
PshAI 1978 GACATGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1641 CAGCTG CAG^CTG
SmlI 1057 CTCAAG C^T[CT][GA]AG
SspBI 965 TGTACA T^GTACA
SspI 1218 AATATT AAT^ATT
StuI 1852 AGGCCT AGG^CCT
Tth111I 1186 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1299 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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BamHI 1163 GGATCC G^GATCC
1563 GGATCC
BbsI 1050 GAAGAC GAAGAC(2/6)
1366 GAAGAC
BpuAI 1050 GAAGAC GAAGAC(2/6)
1366 GAAGAC
BsaWI 425 TCCGGT [AT]^CCGG[AT]
1596 TCCGGA
BseRI 1398 GAGGAG GAGGAG(10/8)
1768 GAGGAG
BstEII 711 GGTGACC G^GT[ACGT]ACC
1279 GGTTACC
BstPI 711 GGTGACC G^GT[ACGT]ACC
1279 GGTTACC
BstYI 1163 GGATCC [GA]^GATC[CT]
1563 GGATCC
DraI 433 TTTAAA TTT^AAA
472 TTTAAA
EarI 370 GAAGAG CTCTTC(1/4)
1765 GAAGAG
EcoO65I 711 GGTGACC G^GT[ACGT]ACC
1279 GGTTACC
HincII 1192 GTCAAC GT[CT]^[GA]AC
1960 GTTGAC
HindII 1192 GTCAAC GT[CT]^[GA]AC
1960 GTTGAC
Ksp632I 370 GAAGAG CTCTTC(1/4)
1765 GAAGAG
KspI 82 CCGCGG CCGC^GG
100 CCGCGG
MflI 1163 GGATCC [GA]^GATC[CT]
1563 GGATCC
NcoI 1469 CCATGG C^CATGG
1800 CCATGG
PpuMI 48 GGGACCT [GA]G^G[AT]CC[CT]
477 AGGACCT
SacII 82 CCGCGG CCGC^GG
100 CCGCGG
SexAI 1187 ACCTGGT A^CC[AT]GGT
1833 ACCTGGT
SmaI 33 CCCGGG CCC^GGG
1915 CCCGGG
TaqI 958 TCGA T^CGA
1330 TCGA
Tsp45I 712 GTGAC ^GT[GC]AC
1069 GTGAC
TthHB8I 958 TCGA T^CGA
1330 TCGA
XhoII 1163 GGATCC [GA]^GATC[CT]
1563 GGATCC
XmaCI 33 CCCGGG C^CCGGG
1915 CCCGGG
XmaI 33 CCCGGG C^CCGGG
1915 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApaI ApoI AspHI AvaI
AvaII BalI BanII BbvI BcnI
BfaI BglI BmyI BpmI BsaAI
BsaJI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
Tru9I TseI Tsp509I TspRI Van91I
XcmI