(Circular) (Six-base) MAPSORT of: pFN21AB6014.seq Check: 6518 from: 1 to: 6799 pFN21AB6014 6799 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4609 278 Size: 53 83 186 936 3073 2468 Fragments arranged by size: 3073 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4142 1937 Size: 2205 4594 Acc65I G'GTAC_C Cuts at: 4120 4120 Size: 6799 AclI AA'CG_TT Cuts at: 1563 4926 5299 1563 Size: 3363 373 3063 Fragments arranged by size: 3363 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2754 3074 3572 4862 6246 6514 1632 Size: 1122 320 498 1290 1384 268 1917 Fragments arranged by size: 1917 1384 1290 1122 498 320 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6602 AflIII A'CryG_T Cuts at: 1203 3711 5698 6675 1203 Size: 2508 1987 977 1327 Fragments arranged by size: 2508 1987 1327 977 AgeI A'CCGG_T Cuts at: 5630 5630 Size: 6799 AhdI GACnn_n'nnGTC Cuts at: 2986 5528 2986 Size: 2542 4257 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6799 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6799 AlwNI CAG_nnn'CTG Cuts at: 3910 4542 6114 3910 Size: 632 1572 4595 Fragments arranged by size: 4595 1572 632 ApaI G_GGCC'C Cuts at: 2314 3854 3921 3928 2314 Size: 1540 67 7 5185 Fragments arranged by size: 5185 1540 67 7 ApaLI G'TGCA_C Cuts at: 3843 4856 6012 3843 Size: 1013 1156 4630 Fragments arranged by size: 4630 1156 1013 ApoI r'AATT_y Cuts at: 1493 2473 2926 2951 4108 4334 4486 6595 1493 Size: 980 453 25 1157 226 152 2109 1697 Fragments arranged by size: 2109 1697 1157 980 453 226 152 25 AseI AT'TA_AT Cuts at: 160 1032 5353 160 Size: 872 4321 1606 Fragments arranged by size: 4321 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6799 AvaI C'yCGr_G Cuts at: 1870 1943 2038 2721 3920 3959 4124 1870 Size: 73 95 683 1199 39 165 4545 Fragments arranged by size: 4545 1199 683 165 95 73 39 BamHI G'GATC_C Cuts at: 3168 3568 4129 4496 3168 Size: 400 561 367 5471 Fragments arranged by size: 5471 561 400 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4120 5575 618 Size: 325 217 627 2333 1455 1842 Fragments arranged by size: 2333 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2071 2314 3854 3921 3928 4118 1391 Size: 680 243 1540 67 7 190 4072 Fragments arranged by size: 4072 1540 680 243 190 67 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3062 3378 928 Size: 971 1163 316 4349 Fragments arranged by size: 4349 1163 971 316 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4990 5024 1903 Size: 34 3053 34 3678 Fragments arranged by size: 3678 3053 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4691 5901 4691 Size: 1210 5589 BclI T'GATC_A Cuts at: 1548 1548 Size: 6799 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2021 2305 Size: 107 122 71 631 605 337 12 284 Cuts at: 2305 3552 5410 136 Size: 1247 1858 1525 Fragments arranged by size: 1858 1525 1247 631 605 337 284 122 107 71 12 BglII A'GATC_T Cuts at: 6794 6794 Size: 6799 BlpI GC'TnA_GC Cuts at: 4216 4216 Size: 6799 Bme1580I G_kGCm'C Cuts at: 1790 2314 3847 3854 3921 3928 4860 6016 1790 Size: 524 1533 7 67 7 932 1156 2573 Fragments arranged by size: 2573 1533 1156 932 524 67 7 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6799 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2344 2586 3857 Size: 416 53 83 399 24 927 242 1271 Cuts at: 3857 4530 5488 442 Size: 673 958 1753 Fragments arranged by size: 1753 1271 958 927 673 416 399 242 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2126 1056 Size: 1070 5729 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2487 2771 3005 3887 3958 5459 1239 Size: 1248 284 234 882 71 1501 2579 Fragments arranged by size: 2579 1501 1248 882 284 234 71 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3047 4924 5789 6087 3047 Size: 1877 865 298 3759 Fragments arranged by size: 3759 1877 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3804 5462 882 Size: 617 2305 1658 2219 Fragments arranged by size: 2305 2219 1658 617 BsaAI yAC'GTr Cuts at: 493 1204 2635 3664 3714 4009 4590 493 Size: 711 1431 1029 50 295 581 2702 Fragments arranged by size: 2702 1431 1029 711 581 295 50 BsaBI GATnn'nnATC Cuts at: 6793 6793 Size: 6799 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2187 4606 4988 5623 Size: 53 83 186 936 654 2419 382 635 Cuts at: 5623 275 Size: 1451 Fragments arranged by size: 2419 1451 936 654 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2430 3601 5232 5630 5904 6051 6462 2430 Size: 1171 1631 398 274 147 411 2767 Fragments arranged by size: 2767 1631 1171 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2988 3018 1178 Size: 30 165 30 1585 30 4959 Fragments arranged by size: 4959 1585 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3418 3788 1186 Size: 2232 370 4197 Fragments arranged by size: 4197 2232 370 BseYI C'CCAG_C Cuts at: 2318 3613 3648 6002 6498 2318 Size: 1295 35 2354 496 2619 Fragments arranged by size: 2619 2354 1295 496 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2276 1163 Size: 573 540 5686 Fragments arranged by size: 5686 573 540 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2016 2244 3107 3544 4095 4249 Size: 275 186 15 228 863 437 551 154 Cuts at: 4249 5010 6038 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 863 761 551 437 275 228 186 154 15 BsiHKAI G_wGCw'C Cuts at: 2728 3450 3669 3847 4860 4945 6016 2728 Size: 722 219 178 1013 85 1071 3511 Fragments arranged by size: 3511 1071 1013 722 219 178 85 BsmI GAATG_Cn' Cuts at: 4310 4403 4310 Size: 93 6706 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2071 2314 2728 3450 3669 3847 3854 Size: 399 281 243 414 722 219 178 7 Cuts at: 3854 3921 3928 4118 4860 4945 6016 1391 Size: 67 7 190 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 722 414 399 281 243 219 190 178 85 67 7 7 BspEI T'CCGG_A Cuts at: 3601 6462 3601 Size: 2861 3938 BspHI T'CATG_A Cuts at: 4688 6418 4688 Size: 1730 5069 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2065 4154 844 Size: 1221 2089 3489 Fragments arranged by size: 3489 2089 1221 BsrBI CCG'CTC Cuts at: 1394 1823 2051 4686 1394 Size: 429 228 2635 3507 Fragments arranged by size: 3507 2635 429 228 BsrDI GCAATG_nn' Cuts at: 65 1248 2867 5294 5468 65 Size: 1183 1619 2427 174 1396 Fragments arranged by size: 2427 1619 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2553 5443 5630 1700 Size: 110 743 2890 187 2869 Fragments arranged by size: 2890 2869 743 187 110 BsrGI T'GTAC_A Cuts at: 96 2970 96 Size: 2874 3925 BssHII G'CGCG_C Cuts at: 1925 2030 1925 Size: 105 6694 BssSI C'ACGA_G Cuts at: 2252 4859 5871 2252 Size: 2607 1012 3180 Fragments arranged by size: 3180 2607 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6799 BstBI TT'CG_AA Cuts at: 5603 5603 Size: 6799 BstEII G'GTnAC_C Cuts at: 1184 2716 3284 1184 Size: 1532 568 4699 Fragments arranged by size: 4699 1532 568 BstYI r'GATC_y Cuts at: 1972 3168 3568 4129 4496 4881 4898 6350 6436 Size: 1196 400 561 367 385 17 1452 86 Cuts at: 6436 6448 6794 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1196 561 400 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2087 2105 2620 3474 3805 4095 Size: 552 939 83 18 515 854 331 290 Cuts at: 4095 513 Size: 3217 Fragments arranged by size: 3217 939 854 552 515 331 290 83 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6060 Fragments arranged by size: 6060 683 56 BtsI GCAGTG_nn' Cuts at: 2281 4315 5109 5129 2281 Size: 2034 794 20 3951 Fragments arranged by size: 3951 2034 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6799 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6764 DraI TTT'AAA Cuts at: 2440 2479 4103 2440 Size: 39 1624 5136 Fragments arranged by size: 5136 1624 39 DraIII CAC_nnn'GTG Cuts at: 3842 3842 Size: 6799 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5806 809 Size: 507 4490 1802 Fragments arranged by size: 4490 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 2241 2278 Size: 54 1425 50 275 18 183 228 37 Cuts at: 2278 3039 3084 3104 3147 3541 3642 3691 3756 Size: 761 45 20 43 394 101 49 65 Cuts at: 3756 4092 4246 5135 6764 8 Size: 336 154 889 1629 43 Fragments arranged by size: 1629 1425 889 761 394 336 275 228 183 154 101 65 54 50 49 45 43 43 37 20 18 EagI C'GGCC_G Cuts at: 1537 1812 2013 2241 3104 3541 4092 4246 1537 Size: 275 201 228 863 437 551 154 4090 Fragments arranged by size: 4090 863 551 437 275 228 201 154 EarI CTCTTCn'nnn_ Cuts at: 1353 2370 3765 4729 1353 Size: 1017 1395 964 3423 Fragments arranged by size: 3423 1395 1017 964 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5386 5760 5906 387 Size: 1064 138 3797 374 146 1280 Fragments arranged by size: 3797 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2487 2754 2771 3005 3074 3572 3887 Size: 393 855 267 17 234 69 498 315 Cuts at: 3887 3958 4862 5459 6246 6514 1239 Size: 71 904 597 787 268 1524 Fragments arranged by size: 1524 904 855 787 597 498 393 315 268 267 234 71 69 17 EcoO109I rG'GnC_Cy Cuts at: 2054 2311 2483 3637 3851 3924 2054 Size: 257 172 1154 214 73 4929 Fragments arranged by size: 4929 1154 257 214 172 73 EcoRI G'AATT_C Cuts at: 4108 4486 4108 Size: 378 6421 FspI TGC'GCA Cuts at: 5305 5305 Size: 6799 HaeII r_GCGC'y Cuts at: 1678 2077 5946 1678 Size: 399 3869 2531 Fragments arranged by size: 3869 2531 399 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2972 3004 3553 3585 3817 Size: 32 135 32 1400 32 549 32 232 Cuts at: 3817 3849 3865 3897 5440 5472 5514 5546 1373 Size: 32 16 32 1543 32 42 32 2626 Fragments arranged by size: 2626 1543 1400 549 232 135 42 32 32 32 32 32 32 32 32 16 HincII GTy'rAC Cuts at: 669 1657 1938 3199 3967 4143 4389 669 Size: 988 281 1261 768 176 246 3079 Fragments arranged by size: 3079 1261 988 768 281 246 176 HindIII A'AGCT_T Cuts at: 748 3584 748 Size: 2836 3963 HpaI GTT'AAC Cuts at: 4389 4389 Size: 6799 KpnI G_GTAC'C Cuts at: 4124 4124 Size: 6799 MfeI C'AATT_G Cuts at: 4398 4398 Size: 6799 MluI A'CGCG_T Cuts at: 6675 6675 Size: 6799 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3105 3232 5913 6097 1305 Size: 1800 127 2681 184 2007 Fragments arranged by size: 2681 2007 1800 184 127 MscI TGG'CCA Cuts at: 10 64 1489 1832 2280 3086 3149 3644 3758 Size: 54 1425 343 448 806 63 495 114 Cuts at: 3758 10 Size: 3051 Fragments arranged by size: 3051 1425 806 495 448 343 114 63 54 MslI CAynn'nnrTG Cuts at: 518 1166 2714 3345 3561 4034 4757 5116 5275 Size: 648 1548 631 216 473 723 359 159 Cuts at: 5275 518 Size: 2042 Fragments arranged by size: 2042 1548 723 648 631 473 359 216 159 MspA1I CmG'CkG Cuts at: 1606 2089 2107 2208 3648 4215 4892 6040 6285 Size: 483 18 101 1440 567 677 1148 245 Cuts at: 6285 1606 Size: 2120 Fragments arranged by size: 2120 1440 1148 677 567 483 245 101 18 NaeI GCC'GGC Cuts at: 2555 2555 Size: 6799 NcoI C'CATG_G Cuts at: 513 1065 2004 3474 3805 513 Size: 552 939 1470 331 3507 Fragments arranged by size: 3507 1470 939 552 331 NdeI CA'TA_TG Cuts at: 387 387 Size: 6799 NgoMIV G'CCGG_C Cuts at: 2553 2553 Size: 6799 NheI G'CTAG_C Cuts at: 1052 2122 1052 Size: 1070 5729 NotI GC'GGCC_GC Cuts at: 4246 4246 Size: 6799 NspI r_CATG'y Cuts at: 1132 1229 4157 5702 1132 Size: 97 2928 1545 2229 Fragments arranged by size: 2928 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6799 PciI A'CATG_T Cuts at: 5698 5698 Size: 6799 PflMI CCAn_nnn'nTGG Cuts at: 1108 2635 3510 3910 4068 1108 Size: 1527 875 400 158 3839 Fragments arranged by size: 3839 1527 875 400 158 PmeI GTTT'AAAC Cuts at: 4103 4103 Size: 6799 PmlI CAC'GTG Cuts at: 3664 3664 Size: 6799 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4868 4900 6381 6413 4868 Size: 32 1481 32 5254 Fragments arranged by size: 5254 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2054 2483 2054 Size: 429 6370 PshAI GACnn'nnGTC Cuts at: 1613 3987 1613 Size: 2374 4425 PsiI TTA'TAA Cuts at: 4369 4369 Size: 6799 PspOMI G'GGCC_C Cuts at: 2310 3850 3917 3924 2310 Size: 1540 67 7 5185 Fragments arranged by size: 5185 1540 67 7 PstI C_TGCA'G Cuts at: 830 2535 3138 3396 4151 830 Size: 1705 603 258 755 3478 Fragments arranged by size: 3478 1705 755 603 258 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6799 PvuII CAG'CTG Cuts at: 3648 3648 Size: 6799 SacII CC_GC'GG Cuts at: 2090 2108 2090 Size: 18 6781 SalI G'TCGA_C Cuts at: 1936 4141 1936 Size: 2205 4594 SbfI CC_TGCA'GG Cuts at: 4151 4151 Size: 6799 ScaI AGT'ACT Cuts at: 5047 6759 5047 Size: 1712 5087 SexAI A'CCwGG_T Cuts at: 3192 3838 3192 Size: 646 6153 SfcI C'TryA_G Cuts at: 826 1045 2531 3134 3392 4147 5282 5963 6154 Size: 219 1486 603 258 755 1135 681 191 Cuts at: 6154 826 Size: 1471 Fragments arranged by size: 1486 1471 1135 755 681 603 258 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6799 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6799 SmaI CCC'GGG Cuts at: 1872 2040 3922 4126 1872 Size: 168 1882 204 4545 Fragments arranged by size: 4545 1882 204 168 SmlI C'TyrA_G Cuts at: 820 1017 1943 3062 4903 5804 6066 820 Size: 197 926 1119 1841 901 262 1553 Fragments arranged by size: 1841 1553 1119 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4590 493 Size: 4097 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6799 SphI G_CATG'C Cuts at: 1132 4157 1132 Size: 3025 3774 SspI AAT'ATT Cuts at: 5 52 3225 4723 5 Size: 47 3173 1498 2081 Fragments arranged by size: 3173 2081 1498 47 StuI AGG'CCT Cuts at: 1517 3859 1517 Size: 2342 4457 StyI C'CwwG_G Cuts at: 513 1065 2004 2668 3474 3805 4238 513 Size: 552 939 664 806 331 433 3074 Fragments arranged by size: 3074 939 806 664 552 433 331 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2185 2505 3357 3417 3828 3836 4826 Size: 1255 605 320 852 60 411 8 990 Cuts at: 4826 4843 4996 5181 5626 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 990 852 605 445 411 320 185 153 60 17 8 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2970 4038 5045 Size: 274 80 33 51 1443 993 1068 1007 Cuts at: 5045 6757 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1068 1007 993 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2177 2209 2664 2696 6759 6791 2177 Size: 32 455 32 4063 32 2185 Fragments arranged by size: 4063 2185 455 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3194 1316 Size: 1878 4921 XbaI T'CTAG_A Cuts at: 4135 4135 Size: 6799 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3157 3588 3911 3157 Size: 431 323 6045 Fragments arranged by size: 6045 431 323 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6799 XmaI C'CCGG_G Cuts at: 1870 2038 3920 4124 1870 Size: 168 1882 204 4545 Fragments arranged by size: 4545 1882 204 168 XmnI GAAnn'nnTTC Cuts at: 3308 4928 3308 Size: 1620 5179 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4607 276 Size: 53 83 186 936 3073 2468 Fragments arranged by size: 3073 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SanDI SapI SfiI SfoI SrfI SwaI