Restriction map for
pFN21AB6014
(Linear) (Six-base) MAPSORT of: KIBB6014.seq Check: 8881 from: 1 to: 2094
KIBB6014.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 749 1069 1567 2094
Size: 749 320 498 527
Fragments arranged by size:
749 527 498 320
AflIII A'CryG_T
Cuts at: 0 1706 2094
Size: 1706 388
AhdI GACnn_n'nnGTC
Cuts at: 0 981 2094
Size: 981 1113
AlwNI CAG_nnn'CTG
Cuts at: 0 1905 2094
Size: 1905 189
ApaI G_GGCC'C
Cuts at: 0 309 1849 1916 1923 2094
Size: 309 1540 67 7 171
Fragments arranged by size:
1540 309 171 67 7
ApaLI G'TGCA_C
Cuts at: 0 1838 2094
Size: 1838 256
ApoI r'AATT_y
Cuts at: 0 468 921 946 2094
Size: 468 453 25 1148
Fragments arranged by size:
1148 468 453 25
AvaI C'yCGr_G
Cuts at: 0 33 716 1915 1954 2094
Size: 33 683 1199 39 140
Fragments arranged by size:
1199 683 140 39 33
BamHI G'GATC_C
Cuts at: 0 1163 1563 2094
Size: 1163 400 531
Fragments arranged by size:
1163 531 400
BanII G_rGCy'C
Cuts at: 0 66 309 1849 1916 1923 2094
Size: 66 243 1540 67 7 171
Fragments arranged by size:
1540 243 171 67 66 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 1057 1373 2094
Size: 1057 316 721
Fragments arranged by size:
1057 721 316
BglI GCCn_nnn'nGGC
Cuts at: 0 16 300 1547 2094
Size: 16 284 1247 547
Fragments arranged by size:
1247 547 284 16
Bme1580I G_kGCm'C
Cuts at: 0 309 1842 1849 1916 1923 2094
Size: 309 1533 7 67 7 171
Fragments arranged by size:
1533 309 171 67 7 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 339 581 1852 2094
Size: 339 242 1271 242
Fragments arranged by size:
1271 339 242 242
BmtI G_CTAG'C
Cuts at: 0 121 2094
Size: 121 1973
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 766 1000 1882 1953 2094
Size: 482 284 234 882 71 141
Fragments arranged by size:
882 482 284 234 141 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1042 2094
Size: 1042 1052
BsaI GGTCTCn'nnnn_
Cuts at: 0 1799 2094
Size: 1799 295
BsaAI yAC'GTr
Cuts at: 0 630 1659 1709 2004 2094
Size: 630 1029 50 295 90
Fragments arranged by size:
1029 630 295 90 50
BsaHI Gr'CG_yC
Cuts at: 0 182 2094
Size: 182 1912
BsaWI w'CCGG_w
Cuts at: 0 425 1596 2094
Size: 425 1171 498
Fragments arranged by size:
1171 498 425
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 983 1013 2094
Size: 983 30 1081
Fragments arranged by size:
1081 983 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1413 1783 2094
Size: 1413 370 311
Fragments arranged by size:
1413 370 311
BseYI C'CCAG_C
Cuts at: 0 313 1608 1643 2094
Size: 313 1295 35 451
Fragments arranged by size:
1295 451 313 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 2094
Size: 271 1823
BsiEI CG_ry'CG
Cuts at: 0 11 239 1102 1539 2090 2094
Size: 11 228 863 437 551 4
Fragments arranged by size:
863 551 437 228 11 4
BsiHKAI G_wGCw'C
Cuts at: 0 723 1445 1664 1842 2094
Size: 723 722 219 178 252
Fragments arranged by size:
723 722 252 219 178
Bsp1286I G_dGCh'C
Cuts at: 0 66 309 723 1445 1664 1842 1849 1916
Size: 66 243 414 722 219 178 7 67
Cuts at: 1916 1923 2094
Size: 7 171
Fragments arranged by size:
722 414 243 219 178 171 67 66
7 7
BspEI T'CCGG_A
Cuts at: 0 1596 2094
Size: 1596 498
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 60 2094
Size: 60 2034
BsrBI CCG'CTC
Cuts at: 0 46 2094
Size: 46 2048
BsrDI GCAATG_nn'
Cuts at: 0 862 2094
Size: 862 1232
BsrFI r'CCGG_y
Cuts at: 0 548 2094
Size: 548 1546
BsrGI T'GTAC_A
Cuts at: 0 965 2094
Size: 965 1129
BssHII G'CGCG_C
Cuts at: 0 25 2094
Size: 25 2069
BssSI C'ACGA_G
Cuts at: 0 247 2094
Size: 247 1847
BstEII G'GTnAC_C
Cuts at: 0 711 1279 2094
Size: 711 568 815
Fragments arranged by size:
815 711 568
BstYI r'GATC_y
Cuts at: 0 1163 1563 2094
Size: 1163 400 531
Fragments arranged by size:
1163 531 400
BtgI C'CryG_G
Cuts at: 0 82 100 615 1469 1800 2094
Size: 82 18 515 854 331 294
Fragments arranged by size:
854 515 331 294 82 18
BtsI GCAGTG_nn'
Cuts at: 0 276 2094
Size: 276 1818
DraI TTT'AAA
Cuts at: 0 435 474 2094
Size: 435 39 1620
Fragments arranged by size:
1620 435 39
DraIII CAC_nnn'GTG
Cuts at: 0 1837 2094
Size: 1837 257
EaeI y'GGCC_r
Cuts at: 0 8 236 273 1034 1079 1099 1142 1536
Size: 8 228 37 761 45 20 43 394
Cuts at: 1536 1637 1686 1751 2087 2094
Size: 101 49 65 336 7
Fragments arranged by size:
761 394 336 228 101 65 49 45
43 37 20 8 7
EagI C'GGCC_G
Cuts at: 0 8 236 1099 1536 2087 2094
Size: 8 228 863 437 551 7
Fragments arranged by size:
863 551 437 228 8 7
EarI CTCTTCn'nnn_
Cuts at: 0 365 1760 2094
Size: 365 1395 334
Fragments arranged by size:
1395 365 334
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 749 766 1000 1069 1567 1882 1953
Size: 482 267 17 234 69 498 315 71
Cuts at: 1953 2094
Size: 141
Fragments arranged by size:
498 482 315 267 234 141 71 69
17
EcoO109I rG'GnC_Cy
Cuts at: 0 49 306 478 1632 1846 1919 2094
Size: 49 257 172 1154 214 73 175
Fragments arranged by size:
1154 257 214 175 172 73 49
HaeII r_GCGC'y
Cuts at: 0 72 2094
Size: 72 2022
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 967 999 1548 1580 1812 1844 1860 1892
Size: 967 32 549 32 232 32 16 32
Cuts at: 1892 2094
Size: 202
Fragments arranged by size:
967 549 232 202 32 32 32 32
16
HincII GTy'rAC
Cuts at: 0 1194 1962 2094
Size: 1194 768 132
Fragments arranged by size:
1194 768 132
HindIII A'AGCT_T
Cuts at: 0 1579 2094
Size: 1579 515
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1100 1227 2094
Size: 1100 127 867
Fragments arranged by size:
1100 867 127
MscI TGG'CCA
Cuts at: 0 275 1081 1144 1639 1753 2094
Size: 275 806 63 495 114 341
Fragments arranged by size:
806 495 341 275 114 63
MslI CAynn'nnrTG
Cuts at: 0 709 1340 1556 2029 2094
Size: 709 631 216 473 65
Fragments arranged by size:
709 631 473 216 65
MspA1I CmG'CkG
Cuts at: 0 84 102 203 1643 2094
Size: 84 18 101 1440 451
Fragments arranged by size:
1440 451 101 84 18
NaeI GCC'GGC
Cuts at: 0 550 2094
Size: 550 1544
NcoI C'CATG_G
Cuts at: 0 1469 1800 2094
Size: 1469 331 294
Fragments arranged by size:
1469 331 294
NgoMIV G'CCGG_C
Cuts at: 0 548 2094
Size: 548 1546
NheI G'CTAG_C
Cuts at: 0 117 2094
Size: 117 1977
PflMI CCAn_nnn'nTGG
Cuts at: 0 630 1505 1905 2063 2094
Size: 630 875 400 158 31
Fragments arranged by size:
875 630 400 158 31
PmlI CAC'GTG
Cuts at: 0 1659 2094
Size: 1659 435
PpuMI rG'GwC_Cy
Cuts at: 0 49 478 2094
Size: 49 429 1616
Fragments arranged by size:
1616 429 49
PshAI GACnn'nnGTC
Cuts at: 0 1982 2094
Size: 1982 112
PspOMI G'GGCC_C
Cuts at: 0 305 1845 1912 1919 2094
Size: 305 1540 67 7 175
Fragments arranged by size:
1540 305 175 67 7
PstI C_TGCA'G
Cuts at: 0 530 1133 1391 2094
Size: 530 603 258 703
Fragments arranged by size:
703 603 530 258
PvuII CAG'CTG
Cuts at: 0 1643 2094
Size: 1643 451
SacII CC_GC'GG
Cuts at: 0 85 103 2094
Size: 85 18 1991
Fragments arranged by size:
1991 85 18
SexAI A'CCwGG_T
Cuts at: 0 1187 1833 2094
Size: 1187 646 261
Fragments arranged by size:
1187 646 261
SfcI C'TryA_G
Cuts at: 0 526 1129 1387 2094
Size: 526 603 258 707
Fragments arranged by size:
707 603 526 258
SmaI CCC'GGG
Cuts at: 0 35 1917 2094
Size: 35 1882 177
Fragments arranged by size:
1882 177 35
SmlI C'TyrA_G
Cuts at: 0 1057 2094
Size: 1057 1037
SspI AAT'ATT
Cuts at: 0 1220 2094
Size: 1220 874
StuI AGG'CCT
Cuts at: 0 1854 2094
Size: 1854 240
StyI C'CwwG_G
Cuts at: 0 663 1469 1800 2094
Size: 663 806 331 294
Fragments arranged by size:
806 663 331 294
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 180 500 1352 1412 1823 1831 2094
Size: 180 320 852 60 411 8 263
Fragments arranged by size:
852 411 320 263 180 60 8
TatI w'GTAC_w
Cuts at: 0 965 2033 2094
Size: 965 1068 61
Fragments arranged by size:
1068 965 61
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 172 204 659 691 2094
Size: 172 32 455 32 1403
Fragments arranged by size:
1403 455 172 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1189 2094
Size: 1189 905
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1152 1583 1906 2094
Size: 1152 431 323 188
Fragments arranged by size:
1152 431 323 188
XmaI C'CCGG_G
Cuts at: 0 33 1915 2094
Size: 33 1882 179
Fragments arranged by size:
1882 179 33
XmnI GAAnn'nnTTC
Cuts at: 0 1303 2094
Size: 1303 791
Enzymes that do cut:
AcuI AflIII AhdI AlwNI ApaI ApaLI ApoI AvaI
BamHI BanII BbsI BglI Bme1580I BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstEII BstYI BtgI BtsI
DraI DraIII EaeI EagI EarI Eco57MI EcoO109I HaeII
Hin4I HincII HindIII MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NheI PflMI PmlI PpuMI PshAI PspOMI
PstI PvuII SacII SexAI SfcI SmaI SmlI SspI
StuI StyI TaqII TatI TstI Tth111I XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AleI AlfI AloI AscI AseI AsiSI AvrII BaeI
BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BplI Bpu10I BsaBI BsiWI BsmI BsmBI
BspHI BstAPI BstBI BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DrdI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HpaI KasI KpnI MfeI MluI
NarI NdeI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PpiI PsiI PspXI PsrI PvuI
RsrII SacI SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SwaI
XbaI XhoI ZraI