Restriction map for
pFN21AB5657
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII AccI AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BfrI BlnI BlpI Bpu1102I
BsaAI BsaHI BsaWI BseAI BsiWI
BsmBI BsoBI Bsp1407I BspEI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstZ17I CelII Cfr10I DraII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRV FseI FspI HaeII HgaI
Hin1I HpaI KasI KspI MfeI
MluI MluNI MroI MscI MspA1I
MunI NaeI NarI NcoI NdeI
NgoMIV NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AocI 130 CCTCAGG CC^T[ACGT]AGG
AspHI 2120 GAGCAC G[AT]GC[AT]^C
AspI 220 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BglI 113 GCCGCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 2881 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1989 GGTCTC GGTCTC(1/5)
BseRI 3072 CTCCTC GAGGAG(10/8)
BsgI 385 GTGCAG GTGCAG(16/14)
BsiEI 1491 CGATCG CG[GA][CT]^CG
BsiHKAI 2120 GAGCAC G[AT]GC[AT]^C
BsmI 3351 GCATTC GAATGC(1/-1)
BspDI 1449 ATCGAT AT^CGAT
BstBI 2939 TTCGAA TT^CGAA
Bsu36I 130 CCTCAGG CC^T[ACGT]AGG
ClaI 1449 ATCGAT AT^CGAT
CpoI 847 CGGTCCG CG^G[AT]CCG
DraIII 359 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 130 CCTCAGG CC^T[ACGT]AGG
EcoT22I 3349 ATGCAT ATGCA^T
FauI 108 CCCGC CCCGC(4/6)
HincII 331 GTTGAC GT[CT]^[GA]AC
HindII 331 GTTGAC GT[CT]^[GA]AC
HindIII 1234 AAGCTT A^AGCTT
MaeII 1119 ACGT A^CGT
MamI 2881 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 183 GCTAGC G^CTAGC
NsiI 3349 ATGCAT ATGCA^T
NspI 923 ACATGC [GA]CATG^[CT]
NspV 2939 TTCGAA TT^CGAA
PflFI 220 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 427 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 79 GACCGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 338 CTGCAG CTGCA^G
PvuI 1491 CGATCG CGAT^CG
RsrII 847 CGGTCCG CG^G[AT]CCG
SfuI 2939 TTCGAA TT^CGAA
Tth111I 220 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 427 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 110 CGCG CG^CG
115 CGCG
AlwNI 814 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1718 CAGGAACTG
Asp700I 490 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
543 GAAGACTTTC
BanII 120 GGGCTC G[GA]GC[CT]^C
1196 GGGCTC
BciVI 2073 GGATAC GTATCC(6/5)
2906 GTATCC
BcnI 81 CCGGG CC^[GC]GG
3361 CCCGG
BglII 644 AGATCT A^GATCT
2108 AGATCT
BspHI 1704 TCATGA T^CATGA
3035 TCATGA
BsrDI 2635 GCAATG GCAATG(2/0)
3176 GCAATG
BstUI 110 CGCG CG^CG
115 CGCG
BstXI 285 CCAAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2594 CCAATATTGTGG
EarI 2425 CTCTTC CTCTTC(1/4)
2732 CTCTTC
EcoRI 1347 GAATTC G^AATTC
3100 GAATTC
EcoT14I 285 CCAAGG C^C[AT][AT]GG
1939 CCAAGG
Ksp632I 2425 CTCTTC CTCTTC(1/4)
2732 CTCTTC
MvnI 110 CGCG CG^CG
115 CGCG
MwoI 113 GCCGCGAGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
275 GCAATTTCAGC
NciI 81 CCGGG CC^[GC]GG
3361 CCCGG
RcaI 1704 TCATGA T^CATGA
3035 TCATGA
SfcI 338 CTGCAG C^T[GA][CT]AG
1053 CTACAG
SspI 211 AATATT AAT^ATT
2596 AATATT
StyI 285 CCAAGG C^C[AT][AT]GG
1939 CCAAGG
XmnI 490 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
543 GAAGACTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
Acc65I AciI AcsI AfaI AluI
AlwI ApoI Asp718I AvaII BanI
BbsI BbvI BclI BfaI BmyI
BpmI BpuAI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI EcoRII
FbaI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI KpnI MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII