(Circular) (Six-base) MAPSORT of: pFN21AB5657.seq Check: 8940 from: 1 to: 8137 pFN21AB5657 8137 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5947 278 Size: 53 83 186 936 4411 2468 Fragments arranged by size: 4411 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5480 1937 Size: 3543 4594 Acc65I G'GTAC_C Cuts at: 3758 3948 4698 5458 3758 Size: 190 750 760 6437 Fragments arranged by size: 6437 760 750 190 AclI AA'CG_TT Cuts at: 1563 6264 6637 1563 Size: 4701 373 3063 Fragments arranged by size: 4701 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4023 4287 6200 7584 7852 1632 Size: 2391 264 1913 1384 268 1917 Fragments arranged by size: 2391 1917 1913 1384 268 264 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7940 AflIII A'CryG_T Cuts at: 1203 7036 8013 1203 Size: 5833 977 1327 Fragments arranged by size: 5833 1327 977 AgeI A'CCGG_T Cuts at: 6968 6968 Size: 8137 AhdI GACnn_n'nnGTC Cuts at: 6866 6866 Size: 8137 AleI CACnn'nnGTG Cuts at: 1166 3081 1166 Size: 1915 6222 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8137 AlwNI CAG_nnn'CTG Cuts at: 2824 3728 5880 7452 2824 Size: 904 2152 1572 3509 Fragments arranged by size: 3509 2152 1572 904 ApaLI G'TGCA_C Cuts at: 6194 7350 6194 Size: 1156 6981 ApoI r'AATT_y Cuts at: 1493 3352 3591 3641 3688 3805 4183 4236 4647 Size: 1859 239 50 47 117 378 53 411 Cuts at: 4647 5105 5446 5672 5824 7933 1493 Size: 458 341 226 152 2109 1697 Fragments arranged by size: 2109 1859 1697 458 411 378 341 239 226 152 117 53 50 47 AseI AT'TA_AT Cuts at: 160 1032 6691 160 Size: 872 5659 1606 Fragments arranged by size: 5659 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8137 AvaI C'yCGr_G Cuts at: 1870 1943 5462 1870 Size: 73 3519 4545 Fragments arranged by size: 4545 3519 73 BamHI G'GATC_C Cuts at: 5467 5834 5467 Size: 367 7770 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2515 3758 3948 4421 4698 Size: 325 217 627 728 1243 190 473 277 Cuts at: 4698 5458 6913 618 Size: 760 1455 1842 Fragments arranged by size: 1842 1455 1243 760 728 627 473 325 277 217 190 BanII G_rGCy'C Cuts at: 1391 2129 3205 5456 1391 Size: 738 1076 2251 4072 Fragments arranged by size: 4072 2251 1076 738 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2555 2625 3119 4879 928 Size: 971 656 70 494 1760 4186 Fragments arranged by size: 4186 1760 971 656 494 70 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6328 6362 1903 Size: 34 4391 34 3678 Fragments arranged by size: 4391 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4072 4922 6029 7239 4072 Size: 850 1107 1210 4970 Fragments arranged by size: 4970 1210 1107 850 BclI T'GATC_A Cuts at: 1548 2797 3505 4312 1548 Size: 1249 708 807 5373 Fragments arranged by size: 5373 1249 807 708 BfrBI ATG'CAT Cuts at: 5356 5356 Size: 8137 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2124 6748 136 Size: 107 122 71 631 605 452 4624 1525 Fragments arranged by size: 4624 1525 631 605 452 122 107 71 BglII A'GATC_T Cuts at: 2649 4113 8132 2649 Size: 1464 4019 2654 Fragments arranged by size: 4019 2654 1464 BlpI GC'TnA_GC Cuts at: 5554 5554 Size: 8137 Bme1580I G_kGCm'C Cuts at: 1790 5190 6198 7354 1790 Size: 3400 1008 1156 2573 Fragments arranged by size: 3400 2573 1156 1008 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8137 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3748 5868 6826 Size: 416 53 83 399 24 2331 2120 958 Cuts at: 6826 442 Size: 1753 Fragments arranged by size: 2331 2120 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2192 1056 Size: 1136 7001 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2227 3636 2227 Size: 1409 6728 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3153 3535 3893 6797 1239 Size: 1914 382 358 2904 2579 Fragments arranged by size: 2904 2579 1914 382 358 Bpu10I CC'TnA_GC Cuts at: 5018 5018 Size: 8137 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4922 5111 5416 6262 7127 7425 4922 Size: 189 305 846 865 298 5634 Fragments arranged by size: 5634 865 846 305 298 189 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4000 6800 882 Size: 617 2501 2800 2219 Fragments arranged by size: 2800 2501 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5928 493 Size: 711 4724 2702 Fragments arranged by size: 4724 2702 711 BsaBI GATnn'nnATC Cuts at: 4890 8131 4890 Size: 3241 4896 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5944 6326 6961 275 Size: 53 83 186 936 4411 382 635 1451 Fragments arranged by size: 4411 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6570 6968 7242 7389 7800 6570 Size: 398 274 147 411 6907 Fragments arranged by size: 6907 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3932 3962 1178 Size: 30 165 30 2529 30 5353 Fragments arranged by size: 5353 2529 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 5068 1186 Size: 3882 4255 BseYI C'CCAG_C Cuts at: 2751 2859 7340 7836 2751 Size: 108 4481 496 3052 Fragments arranged by size: 4481 3052 496 108 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2411 1163 Size: 573 675 6889 Fragments arranged by size: 6889 675 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3499 5587 6348 7376 1540 Size: 275 186 1498 2088 761 1028 2301 Fragments arranged by size: 2301 2088 1498 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4129 6198 6283 7354 4129 Size: 2069 85 1071 4912 Fragments arranged by size: 4912 2069 1071 85 BsmI GAATG_Cn' Cuts at: 5356 5648 5741 5356 Size: 292 93 7752 Fragments arranged by size: 7752 292 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2129 3205 4129 5190 5456 6198 6283 Size: 399 339 1076 924 1061 266 742 85 Cuts at: 6283 7354 1391 Size: 1071 2174 Fragments arranged by size: 2174 1076 1071 1061 924 742 399 339 266 85 BspEI T'CCGG_A Cuts at: 7800 7800 Size: 8137 BspHI T'CATG_A Cuts at: 3709 5040 6026 7756 3709 Size: 1331 986 1730 4090 Fragments arranged by size: 4090 1730 1331 986 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5492 844 Size: 4648 3489 BsrBI CCG'CTC Cuts at: 1394 1823 6024 1394 Size: 429 4201 3507 Fragments arranged by size: 4201 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4647 5188 6632 6806 65 Size: 1183 3399 541 1444 174 1396 Fragments arranged by size: 3399 1444 1396 1183 541 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6781 6968 1700 Size: 110 4971 187 2869 Fragments arranged by size: 4971 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8137 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8137 BssSI C'ACGA_G Cuts at: 6197 7209 6197 Size: 1012 7125 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8137 BstBI TT'CG_AA Cuts at: 4945 6941 4945 Size: 1996 6141 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8137 BstXI CCAn_nnnn'nTGG Cuts at: 2297 4606 2297 Size: 2309 5828 BstYI r'GATC_y Cuts at: 1972 2649 3013 3020 4113 5467 5834 6219 6236 Size: 677 364 7 1093 1354 367 385 17 Cuts at: 6236 7688 7774 7786 8132 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1354 1093 677 385 367 364 346 86 17 12 7 Bsu36I CC'TnA_GG Cuts at: 2136 2136 Size: 8137 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 7585 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7398 Fragments arranged by size: 7398 683 56 BtsI GCAGTG_nn' Cuts at: 2044 2340 2972 4802 5653 6447 6467 2044 Size: 296 632 1830 851 794 20 3714 Fragments arranged by size: 3714 1830 851 794 632 296 20 ClaI AT'CG_AT Cuts at: 1554 3455 1554 Size: 1901 6236 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8102 DraI TTT'AAA Cuts at: 3121 3370 5307 5441 3121 Size: 249 1937 134 5817 Fragments arranged by size: 5817 1937 249 134 DraIII CAC_nnn'GTG Cuts at: 2369 2369 Size: 8137 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7144 809 Size: 507 5828 1802 Fragments arranged by size: 5828 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5584 6473 8102 Size: 54 1425 50 275 18 3754 889 1629 Cuts at: 8102 8 Size: 43 Fragments arranged by size: 3754 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5584 1537 Size: 275 3772 4090 Fragments arranged by size: 4090 3772 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4436 4743 6067 1353 Size: 3083 307 1324 3423 Fragments arranged by size: 3423 3083 1324 307 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6724 7098 7244 387 Size: 1064 138 5135 374 146 1280 Fragments arranged by size: 5135 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3153 3535 3893 4023 4287 6200 6797 Size: 393 1521 382 358 130 264 1913 597 Cuts at: 6797 7584 7852 1239 Size: 787 268 1524 Fragments arranged by size: 1913 1524 1521 787 597 393 382 358 268 264 130 EcoRI G'AATT_C Cuts at: 3352 5105 5446 5824 3352 Size: 1753 341 378 5665 Fragments arranged by size: 5665 1753 378 341 FspI TGC'GCA Cuts at: 6643 6643 Size: 8137 HaeII r_GCGC'y Cuts at: 1678 7284 1678 Size: 5606 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2214 2246 3005 3037 3839 Size: 32 135 32 642 32 759 32 802 Cuts at: 3839 3871 3971 4003 6778 6810 6852 6884 1373 Size: 32 100 32 2775 32 42 32 2626 Fragments arranged by size: 2775 2626 802 759 642 135 100 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2338 5481 5727 669 Size: 988 281 400 3143 246 3079 Fragments arranged by size: 3143 3079 988 400 281 246 HindIII A'AGCT_T Cuts at: 748 3239 748 Size: 2491 5646 HpaI GTT'AAC Cuts at: 5727 5727 Size: 8137 KpnI G_GTAC'C Cuts at: 3762 3952 4702 5462 3762 Size: 190 750 760 6437 Fragments arranged by size: 6437 760 750 190 MfeI C'AATT_G Cuts at: 5736 5736 Size: 8137 MluI A'CGCG_T Cuts at: 8013 8013 Size: 8137 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2379 4576 7251 7435 1305 Size: 1074 2197 2675 184 2007 Fragments arranged by size: 2675 2197 2007 1074 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6315 Fragments arranged by size: 6315 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3081 3711 5060 5193 6095 6454 6613 Size: 648 1915 630 1349 133 902 359 159 Cuts at: 6613 518 Size: 2042 Fragments arranged by size: 2042 1915 1349 902 648 630 359 159 133 MspA1I CmG'CkG Cuts at: 1606 5553 6230 7378 7623 1606 Size: 3947 677 1148 245 2120 Fragments arranged by size: 3947 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7585 NdeI CA'TA_TG Cuts at: 387 387 Size: 8137 NheI G'CTAG_C Cuts at: 1052 2188 1052 Size: 1136 7001 NotI GC'GGCC_GC Cuts at: 5584 5584 Size: 8137 NsiI A_TGCA'T Cuts at: 5358 5358 Size: 8137 NspI r_CATG'y Cuts at: 1132 1229 2932 5495 7040 1132 Size: 97 1703 2563 1545 2229 Fragments arranged by size: 2563 2229 1703 1545 97 PasI CC'CwG_GG Cuts at: 1348 3153 1348 Size: 1805 6332 PciI A'CATG_T Cuts at: 7036 7036 Size: 8137 PflMI CCAn_nnn'nTGG Cuts at: 1108 2438 1108 Size: 1330 6807 PfoI T'CCnGG_A Cuts at: 3130 3181 3870 4779 4914 3130 Size: 51 689 909 135 6353 Fragments arranged by size: 6353 909 689 135 51 PmeI GTTT'AAAC Cuts at: 5441 5441 Size: 8137 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6206 6238 7719 7751 6206 Size: 32 1481 32 6592 Fragments arranged by size: 6592 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2088 1613 Size: 475 7662 PsiI TTA'TAA Cuts at: 5707 5707 Size: 8137 PstI C_TGCA'G Cuts at: 830 2347 5489 830 Size: 1517 3142 3478 Fragments arranged by size: 3478 3142 1517 PvuI CG_AT'CG Cuts at: 2001 3499 2001 Size: 1498 6639 RsrII CG'GwC_CG Cuts at: 2853 2853 Size: 8137 SalI G'TCGA_C Cuts at: 1936 5479 1936 Size: 3543 4594 SbfI CC_TGCA'GG Cuts at: 5489 5489 Size: 8137 ScaI AGT'ACT Cuts at: 6385 8097 6385 Size: 1712 6425 SfcI C'TryA_G Cuts at: 826 1045 2343 3058 5485 6620 7301 7492 826 Size: 219 1298 715 2427 1135 681 191 1471 Fragments arranged by size: 2427 1471 1298 1135 715 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8137 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8137 SmaI CCC'GGG Cuts at: 1872 5464 1872 Size: 3592 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4901 5090 5395 6241 7142 7404 Size: 197 926 2958 189 305 846 901 262 Cuts at: 7404 820 Size: 1553 Fragments arranged by size: 2958 1553 926 901 846 305 262 197 189 SnaBI TAC'GTA Cuts at: 493 5928 493 Size: 5435 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8137 SphI G_CATG'C Cuts at: 1132 5495 1132 Size: 4363 3774 SspI AAT'ATT Cuts at: 5 52 2218 4603 6061 5 Size: 47 2166 2385 1458 2081 Fragments arranged by size: 2385 2166 2081 1458 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8137 StyI C'CwwG_G Cuts at: 513 1065 2290 3944 5576 513 Size: 552 1225 1654 1632 3074 Fragments arranged by size: 3074 1654 1632 1225 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2841 3730 3996 4987 6164 6181 6334 Size: 1255 1261 889 266 991 1177 17 153 Cuts at: 6334 6519 6964 325 Size: 185 445 1498 Fragments arranged by size: 1498 1261 1255 1177 991 889 445 266 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6383 8095 96 Size: 274 80 33 51 1443 4406 1712 138 Fragments arranged by size: 4406 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4035 4067 4137 4169 4572 4604 8097 8129 4035 Size: 32 70 32 403 32 3493 32 4043 Fragments arranged by size: 4043 3493 403 70 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2228 1316 Size: 912 7225 XbaI T'CTAG_A Cuts at: 5473 5473 Size: 8137 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3259 3688 4765 3259 Size: 429 1077 6631 Fragments arranged by size: 6631 1077 429 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8137 XmaI C'CCGG_G Cuts at: 1870 5462 1870 Size: 3592 4545 XmnI GAAnn'nnTTC Cuts at: 2499 2552 6266 2499 Size: 53 3714 4370 Fragments arranged by size: 4370 3714 53 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5945 276 Size: 53 83 186 936 4411 2468 Fragments arranged by size: 4411 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI RsrII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI