Restriction map for
pFN21AB5657
(Linear) (Six-base) MAPSORT of: KIBB5657.seq Check: 8431 from: 1 to: 3432
KIBB5657.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
Acc65I G'GTAC_C
Cuts at: 0 1753 1943 2693 3432
Size: 1753 190 750 739
Fragments arranged by size:
1753 750 739 190
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2018 2282 3432
Size: 2018 264 1150
Fragments arranged by size:
2018 1150 264
AleI CACnn'nnGTG
Cuts at: 0 1076 3432
Size: 1076 2356
AlwNI CAG_nnn'CTG
Cuts at: 0 819 1723 3432
Size: 819 904 1709
Fragments arranged by size:
1709 904 819
ApoI r'AATT_y
Cuts at: 0 1347 1586 1636 1683 1800 2178 2231 2642
Size: 1347 239 50 47 117 378 53 411
Cuts at: 2642 3100 3432
Size: 458 332
Fragments arranged by size:
1347 458 411 378 332 239 117 53
50 47
BanI G'GyrC_C
Cuts at: 0 510 1753 1943 2416 2693 3432
Size: 510 1243 190 473 277 739
Fragments arranged by size:
1243 739 510 473 277 190
BanII G_rGCy'C
Cuts at: 0 124 1200 3432
Size: 124 1076 2232
Fragments arranged by size:
2232 1076 124
BbsI GAAGACnn'nnnn_
Cuts at: 0 550 620 1114 2874 3432
Size: 550 70 494 1760 558
Fragments arranged by size:
1760 558 550 494 70
BciVI GTATCCnnnnn_n'
Cuts at: 0 2067 2917 3432
Size: 2067 850 515
Fragments arranged by size:
2067 850 515
BclI T'GATC_A
Cuts at: 0 792 1500 2307 3432
Size: 792 708 807 1125
Fragments arranged by size:
1125 807 792 708
BfrBI ATG'CAT
Cuts at: 0 3351 3432
Size: 3351 81
BglI GCCn_nnn'nGGC
Cuts at: 0 119 3432
Size: 119 3313
BglII A'GATC_T
Cuts at: 0 644 2108 3432
Size: 644 1464 1324
Fragments arranged by size:
1464 1324 644
Bme1580I G_kGCm'C
Cuts at: 0 3185 3432
Size: 3185 247
BmrI ACTGGGnnnn_n'
Cuts at: 0 1743 3432
Size: 1743 1689
BmtI G_CTAG'C
Cuts at: 0 187 3432
Size: 187 3245
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 222 1631 3432
Size: 222 1409 1801
Fragments arranged by size:
1801 1409 222
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1148 1530 1888 3432
Size: 1148 382 358 1544
Fragments arranged by size:
1544 1148 382 358
Bpu10I CC'TnA_GC
Cuts at: 0 3013 3432
Size: 3013 419
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2917 3106 3411 3432
Size: 2917 189 305 21
Fragments arranged by size:
2917 305 189 21
BsaI GGTCTCn'nnnn_
Cuts at: 0 1995 3432
Size: 1995 1437
BsaBI GATnn'nnATC
Cuts at: 0 2885 3432
Size: 2885 547
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1927 1957 3432
Size: 1927 30 1475
Fragments arranged by size:
1927 1475 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 3063 3432
Size: 3063 369
BseYI C'CCAG_C
Cuts at: 0 746 854 3432
Size: 746 108 2578
Fragments arranged by size:
2578 746 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 3432
Size: 406 3026
BsiEI CG_ry'CG
Cuts at: 0 1494 3432
Size: 1494 1938
BsiHKAI G_wGCw'C
Cuts at: 0 2124 3432
Size: 2124 1308
BsmI GAATG_Cn'
Cuts at: 0 3351 3432
Size: 3351 81
Bsp1286I G_dGCh'C
Cuts at: 0 124 1200 2124 3185 3432
Size: 124 1076 924 1061 247
Fragments arranged by size:
1076 1061 924 247 124
BspHI T'CATG_A
Cuts at: 0 1704 3035 3432
Size: 1704 1331 397
Fragments arranged by size:
1704 1331 397
BsrDI GCAATG_nn'
Cuts at: 0 2642 3183 3432
Size: 2642 541 249
Fragments arranged by size:
2642 541 249
BstBI TT'CG_AA
Cuts at: 0 2940 3432
Size: 2940 492
BstXI CCAn_nnnn'nTGG
Cuts at: 0 292 2601 3432
Size: 292 2309 831
Fragments arranged by size:
2309 831 292
BstYI r'GATC_y
Cuts at: 0 644 1008 1015 2108 3432
Size: 644 364 7 1093 1324
Fragments arranged by size:
1324 1093 644 364 7
Bsu36I CC'TnA_GG
Cuts at: 0 131 3432
Size: 131 3301
BtsI GCAGTG_nn'
Cuts at: 0 39 335 967 2797 3432
Size: 39 296 632 1830 635
Fragments arranged by size:
1830 635 632 296 39
ClaI AT'CG_AT
Cuts at: 0 1450 3432
Size: 1450 1982
DraI TTT'AAA
Cuts at: 0 1116 1365 3302 3432
Size: 1116 249 1937 130
Fragments arranged by size:
1937 1116 249 130
DraIII CAC_nnn'GTG
Cuts at: 0 364 3432
Size: 364 3068
EarI CTCTTCn'nnn_
Cuts at: 0 2431 2738 3432
Size: 2431 307 694
Fragments arranged by size:
2431 694 307
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1148 1530 1888 2018 2282 3432
Size: 1148 382 358 130 264 1150
Fragments arranged by size:
1150 1148 382 358 264 130
EcoRI G'AATT_C
Cuts at: 0 1347 3100 3432
Size: 1347 1753 332
Fragments arranged by size:
1753 1347 332
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 209 241 1000 1032 1834 1866 1966 1998
Size: 209 32 759 32 802 32 100 32
Cuts at: 1998 3432
Size: 1434
Fragments arranged by size:
1434 802 759 209 100 32 32 32
32
HincII GTy'rAC
Cuts at: 0 333 3432
Size: 333 3099
HindIII A'AGCT_T
Cuts at: 0 1234 3432
Size: 1234 2198
KpnI G_GTAC'C
Cuts at: 0 1757 1947 2697 3432
Size: 1757 190 750 735
Fragments arranged by size:
1757 750 735 190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 2571 3432
Size: 374 2197 861
Fragments arranged by size:
2197 861 374
MslI CAynn'nnrTG
Cuts at: 0 1076 1706 3055 3188 3432
Size: 1076 630 1349 133 244
Fragments arranged by size:
1349 1076 630 244 133
NheI G'CTAG_C
Cuts at: 0 183 3432
Size: 183 3249
NsiI A_TGCA'T
Cuts at: 0 3353 3432
Size: 3353 79
NspI r_CATG'y
Cuts at: 0 927 3432
Size: 927 2505
PasI CC'CwG_GG
Cuts at: 0 1148 3432
Size: 1148 2284
PflMI CCAn_nnn'nTGG
Cuts at: 0 433 3432
Size: 433 2999
PfoI T'CCnGG_A
Cuts at: 0 1125 1176 1865 2774 2909 3432
Size: 1125 51 689 909 135 523
Fragments arranged by size:
1125 909 689 523 135 51
PshAI GACnn'nnGTC
Cuts at: 0 83 3432
Size: 83 3349
PstI C_TGCA'G
Cuts at: 0 342 3432
Size: 342 3090
PvuI CG_AT'CG
Cuts at: 0 1494 3432
Size: 1494 1938
RsrII CG'GwC_CG
Cuts at: 0 848 3432
Size: 848 2584
SfcI C'TryA_G
Cuts at: 0 338 1053 3432
Size: 338 715 2379
Fragments arranged by size:
2379 715 338
SmlI C'TyrA_G
Cuts at: 0 2896 3085 3390 3432
Size: 2896 189 305 42
Fragments arranged by size:
2896 305 189 42
SspI AAT'ATT
Cuts at: 0 213 2598 3432
Size: 213 2385 834
Fragments arranged by size:
2385 834 213
StyI C'CwwG_G
Cuts at: 0 285 1939 3432
Size: 285 1654 1493
Fragments arranged by size:
1654 1493 285
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 836 1725 1991 2982 3432
Size: 836 889 266 991 450
Fragments arranged by size:
991 889 836 450 266
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2030 2062 2132 2164 2567 2599 3432
Size: 2030 32 70 32 403 32 833
Fragments arranged by size:
2030 833 403 70 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 223 3432
Size: 223 3209
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1254 1683 2760 3432
Size: 1254 429 1077 672
Fragments arranged by size:
1254 1077 672 429
XmnI GAAnn'nnTTC
Cuts at: 0 494 547 3432
Size: 494 53 2885
Fragments arranged by size:
2885 494 53
Enzymes that do cut:
Acc65I AcuI AleI AlwNI ApoI BanI BanII BbsI
BciVI BclI BfrBI BglI BglII Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BsrDI
BstBI BstXI BstYI Bsu36I BtsI ClaI DraI DraIII
EarI Eco57MI EcoRI Hin4I HincII HindIII KpnI MmeI
MslI NheI NsiI NspI PasI PflMI PfoI PshAI
PstI PvuI RsrII SfcI SmlI SspI StyI TaqII
TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BlpI
BmgBI BsaAI BsaHI BsaWI BsiWI BsmBI BspEI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstZ17I
BtgI BtgZI CspCI DrdI EaeI EagI EciI EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HaeII
HpaI KasI MfeI MluI MscI MspA1I NaeI NarI
NcoI NdeI NgoMIV NotI NruI PacI PciI PmeI
PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PvuII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI TatI XbaI XhoI XmaI ZraI