Restriction map for
pFN21AB5628
##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AccI AclI AflII AocI AscI
AviII BbvCI BcgI BciVI BclI
BfrI BsaBI BsiWI Bsp1407I BspDI
BsrGI BssHII Bst1107I BstXI BstZ17I
Bsu36I ClaI CpoI Eco81I EcoRV
FbaI FseI FspI HindIII HpaI
MamI MfeI MluI MunI NcoI
NdeI NruI PacI PmeI PshAI
Psp1406I PvuI RsrII SalI ScaI
SfiI SgrAI SnaBI SpeI SspBI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AatII 2377 GACGTC GACGT^C
Acc65I 4114 GGTACC G^GTACC
AgeI 3128 ACCGGT A^CCGGT
Aor51HI 1604 AGCGCT AGC^GCT
Asp700I 2202 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4114 GGTACC G^GTACC
AvrII 1503 CCTAGG C^CTAGG
BbsI 3634 GAAGAC GAAGAC(2/6)
BlnI 1503 CCTAGG C^CTAGG
BpuAI 3634 GAAGAC GAAGAC(2/6)
BsaI 182 GAGACC GGTCTC(1/5)
BsmBI 3915 CGTCTC CGTCTC(1/5)
BspLU11I 2402 ACATGT A^CATGT
DraI 675 TTTAAA TTT^AAA
DrdI 635 GACTAGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1604 AGCGCT AGC^GCT
EcoT22I 2490 ATGCAT ATGCA^T
KpnI 4114 GGTACC GGTAC^C
NheI 2367 GCTAGC G^CTAGC
NotI 2998 GCGGCCGC GC^GGCCGC
NsiI 2490 ATGCAT ATGCA^T
PaeR7I 1338 CTCGAG C^TCGAG
PinAI 3128 ACCGGT A^CCGGT
SbfI 2846 CCTGCAGG CCTGCA^GG
Sse8387I 2846 CCTGCAGG CCTGCA^GG
SspI 2246 AATATT AAT^ATT
XhoI 1338 CTCGAG C^TCGAG
XmnI 2202 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 1431 TCCGGA T^CCGGA
2965 TCCGGA
AflIII 1013 ACGTGT A^C[GA][CT]GT
2402 ACATGT
AhdI 2956 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3805 GACAGCCCGTC
Alw44I 1086 GTGCAC G^TGCAC
2516 GTGCAC
ApaLI 1086 GTGCAC G^TGCAC
2516 GTGCAC
AseI 246 ATTAAT AT^TAAT
3544 ATTAAT
AsnI 246 ATTAAT AT^TAAT
3544 ATTAAT
AspEI 2956 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3805 GACAGCCCGTC
BalI 3191 TGGCCA TGG^CCA
3710 TGGCCA
BamHI 3312 GGATCC G^GATCC
3480 GGATCC
BglI 1352 GCCCCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2128 GCCAGCTGGGC
BglII 2426 AGATCT A^GATCT
3944 AGATCT
BseAI 1431 TCCGGA T^CCGGA
2965 TCCGGA
BspEI 1431 TCCGGA T^CCGGA
2965 TCCGGA
BspMI 3077 ACCTGC ACCTGC(4/8)
2535 GCAGGT
BsrBI 1463 CCGCTC CCGCTC(-3/-3)
2977 GAGCGG
BssSI 726 CACGAG CACGAG(-5/-1)
2694 CTCGTG
BstBI 304 TTCGAA TT^CGAA
1856 TTCGAA
BstEII 813 GGTGACC G^GT[ACGT]ACC
942 GGTTACC
BstPI 813 GGTGACC G^GT[ACGT]ACC
942 GGTTACC
DraIII 119 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
274 CACATGGTG
Eam1105I 2956 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3805 GACAGCCCGTC
EcoO65I 813 GGTGACC G^GT[ACGT]ACC
942 GGTTACC
EcoRI 1120 GAATTC G^AATTC
2206 GAATTC
HincII 2022 GTTGAC GT[CT]^[GA]AC
2344 GTCAAC
HindII 2022 GTTGAC GT[CT]^[GA]AC
2344 GTCAAC
KspI 765 CCGCGG CCGC^GG
3002 CCGCGG
MluNI 3191 TGGCCA TGG^CCA
3710 TGGCCA
MroI 1431 TCCGGA T^CCGGA
2965 TCCGGA
MscI 3191 TGGCCA TGG^CCA
3710 TGGCCA
NaeI 88 GCCGGC GCC^GGC
2102 GCCGGC
NgoMIV 88 GCCGGC G^CCGGC
2102 GCCGGC
NspV 304 TTCGAA TT^CGAA
1856 TTCGAA
PshBI 246 ATTAAT AT^TAAT
3544 ATTAAT
SacII 765 CCGCGG CCGC^GG
3002 CCGCGG
SapI 1299 GAAGAGC GCTCTTC(1/4)
2644 GAAGAGC
SexAI 3638 ACCTGGT A^CC[AT]GGT
4110 ACCAGGT
SfuI 304 TTCGAA TT^CGAA
1856 TTCGAA
XcmI 1106 CCAATGGCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3194 CCAGATACGGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AspI AvaI AvaII BanI
BanII BbeI BbrPI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BsaAI BsaHI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspHI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI SphI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XhoII XmaCI XmaI