(Circular) (Six-base) MAPSORT of: pFN21AB5628.seq Check: 2493 from: 1 to: 8833 pFN21AB5628 8833 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4386 6643 278 Size: 53 83 186 936 2850 2257 2468 Fragments arranged by size: 2850 2468 2257 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6176 1937 Size: 4239 4594 Acc65I G'GTAC_C Cuts at: 6119 6154 6119 Size: 35 8798 AclI AA'CG_TT Cuts at: 1563 6960 7333 1563 Size: 5397 373 3063 Fragments arranged by size: 5397 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2313 2384 2890 4064 4493 6896 8280 8548 Size: 681 71 506 1174 429 2403 1384 268 Cuts at: 8548 1632 Size: 1917 Fragments arranged by size: 2403 1917 1384 1174 681 506 429 268 71 AfeI AGC'GCT Cuts at: 3611 3611 Size: 8833 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8636 AflIII A'CryG_T Cuts at: 1203 3018 4407 7732 8709 1203 Size: 1815 1389 3325 977 1327 Fragments arranged by size: 3325 1815 1389 1327 977 AgeI A'CCGG_T Cuts at: 5133 7664 5133 Size: 2531 6302 AhdI GACnn_n'nnGTC Cuts at: 4966 5815 7562 4966 Size: 849 1747 6237 Fragments arranged by size: 6237 1747 849 AleI CACnn'nnGTG Cuts at: 1166 2841 4590 5177 1166 Size: 1675 1749 587 4822 Fragments arranged by size: 4822 1749 1675 587 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 6086 1184 Size: 4902 3931 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5679 5711 5679 Size: 32 8801 AlwNI CAG_nnn'CTG Cuts at: 3489 4426 5711 6576 8148 3489 Size: 937 1285 865 1572 4174 Fragments arranged by size: 4174 1572 1285 937 865 ApaI G_GGCC'C Cuts at: 3211 3429 4303 5898 3211 Size: 218 874 1595 6146 Fragments arranged by size: 6146 1595 874 218 ApaLI G'TGCA_C Cuts at: 3091 4521 6890 8046 3091 Size: 1430 2369 1156 3878 Fragments arranged by size: 3878 2369 1430 1156 ApoI r'AATT_y Cuts at: 1493 2439 3125 3893 4211 5386 6142 6368 6520 Size: 946 686 768 318 1175 756 226 152 Cuts at: 6520 8629 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1175 946 768 756 686 318 226 152 AseI AT'TA_AT Cuts at: 160 1032 2252 5550 7387 160 Size: 872 1220 3298 1837 1606 Fragments arranged by size: 3298 1837 1606 1220 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8833 AvaI C'yCGr_G Cuts at: 1870 1943 2082 2464 3343 4302 4868 5827 5873 Size: 73 139 382 879 959 566 959 46 Cuts at: 5873 6158 1870 Size: 285 4545 Fragments arranged by size: 4545 959 959 879 566 382 285 139 73 46 AvrII C'CTAG_G Cuts at: 3508 3508 Size: 8833 BamHI G'GATC_C Cuts at: 5317 5485 6163 6530 5317 Size: 168 678 367 7620 Fragments arranged by size: 7620 678 367 168 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2122 2784 2996 3395 3532 Size: 325 217 627 335 662 212 399 137 Cuts at: 3532 3578 4034 4110 4864 5267 5939 6119 6154 Size: 46 456 76 754 403 672 180 35 Cuts at: 6154 7609 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 754 672 662 627 456 403 399 335 325 217 212 180 137 76 46 35 BanII G_rGCy'C Cuts at: 1391 2159 2493 2738 3211 3344 3429 3455 3557 Size: 768 334 245 473 133 85 26 102 Cuts at: 3557 3890 4303 4814 5256 5834 5898 6152 1391 Size: 333 413 511 442 578 64 254 4072 Fragments arranged by size: 4072 768 578 511 473 442 413 334 333 254 245 133 102 85 64 26 BbeI G_GCGC'C Cuts at: 2788 3000 4038 4114 5271 2788 Size: 212 1038 76 1157 6350 Fragments arranged by size: 6350 1157 1038 212 76 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 5646 928 Size: 971 3747 4115 Fragments arranged by size: 4115 3747 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7024 7058 1903 Size: 34 5087 34 3678 Fragments arranged by size: 5087 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6725 7935 6725 Size: 1210 7623 BclI T'GATC_A Cuts at: 1548 1548 Size: 8833 BfrBI ATG'CAT Cuts at: 4497 4497 Size: 8833 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3363 4139 7444 Size: 107 122 71 631 605 1691 776 3305 Cuts at: 7444 136 Size: 1525 Fragments arranged by size: 3305 1691 1525 776 631 605 122 107 71 BglII A'GATC_T Cuts at: 4431 5949 8828 4431 Size: 1518 2879 4436 Fragments arranged by size: 4436 2879 1518 BlpI GC'TnA_GC Cuts at: 2135 2852 5833 6250 2135 Size: 717 2981 417 4718 Fragments arranged by size: 4718 2981 717 417 Bme1580I G_kGCm'C Cuts at: 1790 2921 3095 3211 3400 3429 3537 3581 4303 Size: 1131 174 116 189 29 108 44 722 Cuts at: 4303 4525 5898 5942 6894 8050 1790 Size: 222 1373 44 952 1156 2573 Fragments arranged by size: 2573 1373 1156 1131 952 722 222 189 174 116 108 44 44 29 BmgBI CAC'GTC Cuts at: 1318 3936 1318 Size: 2618 6215 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3432 5905 6564 Size: 416 53 83 399 24 2015 2473 659 Cuts at: 6564 7522 442 Size: 958 1753 Fragments arranged by size: 2473 2015 1753 958 659 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4376 1056 Size: 3320 5513 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5782 5982 5782 Size: 200 8633 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2205 2765 2889 4573 5380 5688 5824 6109 Size: 966 560 124 1684 807 308 136 285 Cuts at: 6109 7493 1239 Size: 1384 2579 Fragments arranged by size: 2579 1684 1384 966 807 560 308 285 136 124 Bpu10I CC'TnA_GC Cuts at: 4070 5630 4070 Size: 1560 7273 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3568 4019 5132 6958 7823 8121 3568 Size: 451 1113 1826 865 298 4280 Fragments arranged by size: 4280 1826 1113 865 451 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2181 7496 882 Size: 617 682 5315 2219 Fragments arranged by size: 5315 2219 682 617 BsaAI yAC'GTr Cuts at: 493 1204 3019 3628 6013 6624 493 Size: 711 1815 609 2385 611 2702 Fragments arranged by size: 2702 2385 1815 711 611 609 BsaBI GATnn'nnATC Cuts at: 8827 8827 Size: 8833 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2298 2785 2997 3134 Size: 53 83 186 936 765 487 212 137 Cuts at: 3134 3231 4035 4111 4383 4767 5268 6640 7022 Size: 97 804 76 272 384 501 1372 382 Cuts at: 7022 7657 275 Size: 635 1451 Fragments arranged by size: 1451 1372 936 804 765 635 501 487 384 382 272 212 186 137 97 83 76 53 BsaWI w'CCGG_w Cuts at: 3436 4970 5133 7266 7664 7938 8085 8496 3436 Size: 1534 163 2133 398 274 147 411 3773 Fragments arranged by size: 3773 2133 1534 411 398 274 163 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2696 2726 3629 3659 5679 Size: 30 165 30 1293 30 903 30 2020 Cuts at: 5679 5709 1178 Size: 30 4302 Fragments arranged by size: 4302 2020 1293 903 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3002 3005 3314 3565 4138 5324 5582 1186 Size: 1816 3 309 251 573 1186 258 4437 Fragments arranged by size: 4437 1816 1186 573 309 258 251 3 BseYI C'CCAG_C Cuts at: 2015 3399 3538 4091 4137 4368 4547 4654 5769 Size: 1384 139 553 46 231 179 107 1115 Cuts at: 5769 5888 8036 8532 2015 Size: 119 2148 496 2316 Fragments arranged by size: 2316 2148 1384 1115 553 496 231 179 139 119 107 46 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2867 4118 4254 5033 5887 1163 Size: 573 1131 1251 136 779 854 4109 Fragments arranged by size: 4109 1251 1131 854 779 573 136 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2305 2529 2892 2929 3099 4636 Size: 275 186 304 224 363 37 170 1537 Cuts at: 4636 5007 5755 6283 7044 8072 1540 Size: 371 748 528 761 1028 2301 Fragments arranged by size: 2301 1537 1028 761 748 528 371 363 304 275 224 186 170 37 BsiHKAI G_wGCw'C Cuts at: 2136 2267 2738 2858 3095 3344 3415 3557 3633 Size: 131 471 120 237 249 71 142 76 Cuts at: 3633 4525 4721 4749 6012 6894 6979 8050 2136 Size: 892 196 28 1263 882 85 1071 2919 Fragments arranged by size: 2919 1263 1071 892 882 471 249 237 196 142 131 120 85 76 71 28 BsmI GAATG_Cn' Cuts at: 3796 3834 4499 4899 6344 6437 3796 Size: 38 665 400 1445 93 6192 Fragments arranged by size: 6192 1445 665 400 93 38 BsmBI CGTCTCn'nnnn_ Cuts at: 5926 5926 Size: 8833 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2136 2159 2267 2493 2738 2858 2921 Size: 399 346 23 108 226 245 120 63 Cuts at: 2921 3095 3211 3344 3400 3415 3429 3455 3537 Size: 174 116 133 56 15 14 26 82 Cuts at: 3537 3557 3581 3633 3890 4303 4525 4721 4749 Size: 20 24 52 257 413 222 196 28 Cuts at: 4749 4814 5256 5834 5898 5942 6012 6152 6894 Size: 65 442 578 64 44 70 140 742 Cuts at: 6894 6979 8050 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 578 442 413 399 346 257 245 226 222 196 174 140 133 120 116 108 85 82 70 65 64 63 56 52 44 28 26 24 23 20 15 14 BspEI T'CCGG_A Cuts at: 3436 4970 8496 3436 Size: 1534 3526 3773 Fragments arranged by size: 3773 3526 1534 BspHI T'CATG_A Cuts at: 2145 2233 2614 2650 4443 6722 8452 2145 Size: 88 381 36 1793 2279 1730 2526 Fragments arranged by size: 2526 2279 1793 1730 381 88 36 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4531 5091 6188 844 Size: 3687 560 1097 3489 Fragments arranged by size: 3687 3489 1097 560 BsrBI CCG'CTC Cuts at: 1394 1823 3470 4984 6720 1394 Size: 429 1647 1514 1736 3507 Fragments arranged by size: 3507 1736 1647 1514 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2036 3066 4084 7328 7502 65 Size: 1183 788 1030 1018 3244 174 1396 Fragments arranged by size: 3244 1396 1183 1030 1018 788 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2093 2928 4107 5133 7477 7664 1700 Size: 110 283 835 1179 1026 2344 187 2869 Fragments arranged by size: 2869 2344 1179 1026 835 283 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8833 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8833 BssSI C'ACGA_G Cuts at: 2731 4699 6893 7905 2731 Size: 1968 2194 1012 3659 Fragments arranged by size: 3659 2194 1968 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2669 3708 4426 794 Size: 1875 1039 718 5201 Fragments arranged by size: 5201 1875 1039 718 BstBI TT'CG_AA Cuts at: 2310 3862 7637 2310 Size: 1552 3775 3506 Fragments arranged by size: 3775 3506 1552 BstEII G'GTnAC_C Cuts at: 1184 2818 2947 1184 Size: 1634 129 7070 Fragments arranged by size: 7070 1634 129 BstYI r'GATC_y Cuts at: 1972 2510 3054 3738 3959 4431 5116 5317 5485 Size: 538 544 684 221 472 685 201 168 Cuts at: 5485 5949 6163 6530 6915 6932 8384 8470 8482 Size: 464 214 367 385 17 1452 86 12 Cuts at: 8482 8828 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 685 684 544 538 472 464 385 367 346 221 214 201 168 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2770 3582 5007 5190 513 Size: 552 1705 812 1425 183 4156 Fragments arranged by size: 4156 1705 1425 812 552 183 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2705 487 Size: 683 56 1479 6615 Fragments arranged by size: 6615 1479 683 56 BtsI GCAGTG_nn' Cuts at: 3650 3716 4891 5789 6349 7143 7163 3650 Size: 66 1175 898 560 794 20 5320 Fragments arranged by size: 5320 1175 898 794 560 66 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8833 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8798 DraI TTT'AAA Cuts at: 2682 6137 2682 Size: 3455 5378 DraIII CAC_nnn'GTG Cuts at: 2129 2284 2129 Size: 155 8678 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2646 7840 809 Size: 507 1330 5194 1802 Fragments arranged by size: 5194 1802 1330 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2302 2526 2877 Size: 54 1425 50 275 18 472 224 351 Cuts at: 2877 2889 3096 4633 5004 5196 5715 5752 6015 Size: 12 207 1537 371 192 519 37 263 Cuts at: 6015 6280 7169 8798 8 Size: 265 889 1629 43 Fragments arranged by size: 1629 1537 1425 889 519 472 371 351 275 265 263 224 207 192 54 50 43 37 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2302 2526 2889 3096 4633 5004 5752 Size: 275 490 224 363 207 1537 371 748 Cuts at: 5752 6280 1537 Size: 528 4090 Fragments arranged by size: 4090 1537 748 528 490 371 363 275 224 207 EarI CTCTTCn'nnn_ Cuts at: 1353 3299 3794 4644 5021 5951 6763 1353 Size: 1946 495 850 377 930 812 3423 Fragments arranged by size: 3423 1946 930 850 812 495 377 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4520 7420 7794 7940 387 Size: 1064 138 2931 2900 374 146 1280 Fragments arranged by size: 2931 2900 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2736 3342 3555 2736 Size: 606 213 8014 Fragments arranged by size: 8014 606 213 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2205 2313 2384 2765 2889 2890 4064 Size: 393 573 108 71 381 124 1 1174 Cuts at: 4064 4493 4573 5380 5688 5824 6109 6896 7493 Size: 429 80 807 308 136 285 787 597 Cuts at: 7493 8280 8548 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1174 807 787 787 597 573 429 393 381 308 285 268 136 124 108 80 71 1 EcoNI CCTnn'n_nnAGG Cuts at: 2981 4163 4844 5325 5930 2981 Size: 1182 681 481 605 5884 Fragments arranged by size: 5884 1182 681 605 481 EcoO109I rG'GnC_Cy Cuts at: 3316 3426 3493 4168 4299 4543 4728 5080 5895 Size: 110 67 675 131 244 185 352 815 Cuts at: 5895 6104 3316 Size: 209 6045 Fragments arranged by size: 6045 815 675 352 244 209 185 131 110 67 EcoRI G'AATT_C Cuts at: 3125 4211 6142 6520 3125 Size: 1086 1931 378 5438 Fragments arranged by size: 5438 1931 1086 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2920 4963 5119 2920 Size: 2043 156 6634 Fragments arranged by size: 6634 2043 156 FspI TGC'GCA Cuts at: 7339 7339 Size: 8833 HaeII r_GCGC'y Cuts at: 1678 2788 2913 3000 3613 4038 4114 4159 5271 Size: 1110 125 87 613 425 76 45 1112 Cuts at: 5271 5742 5766 7980 1678 Size: 471 24 2214 2531 Fragments arranged by size: 2531 2214 1112 1110 613 471 425 125 87 76 45 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2585 2617 3653 3680 3685 Size: 32 135 32 1013 32 1036 27 5 Cuts at: 3685 3712 3960 3986 3992 4018 4423 4455 4904 Size: 27 248 26 6 26 405 32 449 Cuts at: 4904 4936 4952 4984 5801 5833 7474 7506 7548 Size: 32 16 32 817 32 1641 32 42 Cuts at: 7548 7580 1373 Size: 32 2626 Fragments arranged by size: 2626 1641 1036 1013 817 449 405 248 135 42 32 32 32 32 32 32 32 32 32 27 27 26 26 16 6 5 HincII GTy'rAC Cuts at: 669 1657 1938 4029 4351 6177 6423 669 Size: 988 281 2091 322 1826 246 3079 Fragments arranged by size: 3079 2091 1826 988 322 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8833 HpaI GTT'AAC Cuts at: 6423 6423 Size: 8833 KasI G'GCGC_C Cuts at: 2784 2996 4034 4110 5267 2784 Size: 212 1038 76 1157 6350 Fragments arranged by size: 6350 1157 1038 212 76 KpnI G_GTAC'C Cuts at: 6123 6158 6123 Size: 35 8798 MfeI C'AATT_G Cuts at: 6432 6432 Size: 8833 MluI A'CGCG_T Cuts at: 8709 8709 Size: 8833 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2527 2763 2775 4824 7947 8131 1305 Size: 1222 236 12 2049 3123 184 2007 Fragments arranged by size: 3123 2049 2007 1222 236 184 12 MscI TGG'CCA Cuts at: 10 64 1489 1832 5198 5717 10 Size: 54 1425 343 3366 519 3126 Fragments arranged by size: 3366 3126 1425 519 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2238 2841 3855 4590 4779 5177 6791 Size: 648 1072 603 1014 735 189 398 1614 Cuts at: 6791 7150 7309 518 Size: 359 159 2042 Fragments arranged by size: 2042 1614 1072 1014 735 648 603 398 359 189 159 MspA1I CmG'CkG Cuts at: 1606 2139 2772 2851 3276 3787 4137 4426 4483 Size: 533 633 79 425 511 350 289 57 Cuts at: 4483 5009 5296 5381 5708 5892 6249 6926 8074 Size: 526 287 85 327 184 357 677 1148 Cuts at: 8074 8319 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 633 533 526 511 425 357 350 327 289 287 245 184 85 79 57 NaeI GCC'GGC Cuts at: 2095 4109 2095 Size: 2014 6819 NarI GG'CG_CC Cuts at: 2785 2997 4035 4111 5268 2785 Size: 212 1038 76 1157 6350 Fragments arranged by size: 6350 1157 1038 212 76 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 8281 NdeI CA'TA_TG Cuts at: 387 387 Size: 8833 NgoMIV G'CCGG_C Cuts at: 2093 4107 2093 Size: 2014 6819 NheI G'CTAG_C Cuts at: 1052 4372 1052 Size: 3320 5513 NotI GC'GGCC_GC Cuts at: 5004 6280 5004 Size: 1276 7557 NsiI A_TGCA'T Cuts at: 4499 4499 Size: 8833 NspI r_CATG'y Cuts at: 1132 1229 2584 2782 3205 4411 4778 6191 7736 Size: 97 1355 198 423 1206 367 1413 1545 Cuts at: 7736 1132 Size: 2229 Fragments arranged by size: 2229 1545 1413 1355 1206 423 367 198 97 PasI CC'CwG_GG Cuts at: 1348 3405 1348 Size: 2057 6776 PciI A'CATG_T Cuts at: 4407 7732 4407 Size: 3325 5508 PflMI CCAn_nnn'nTGG Cuts at: 1108 2042 2815 5998 1108 Size: 934 773 3183 3943 Fragments arranged by size: 3943 3183 934 773 PfoI T'CCnGG_A Cuts at: 3633 3812 4527 4908 5796 3633 Size: 179 715 381 888 6670 Fragments arranged by size: 6670 888 715 381 179 PmeI GTTT'AAAC Cuts at: 6137 6137 Size: 8833 PmlI CAC'GTG Cuts at: 3019 3628 6013 3019 Size: 609 2385 5839 Fragments arranged by size: 5839 2385 609 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5679 5711 6902 6934 8415 8447 5679 Size: 32 1191 32 1481 32 6065 Fragments arranged by size: 6065 1481 1191 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3316 3493 4168 4543 4728 5080 6104 3316 Size: 177 675 375 185 352 1024 6045 Fragments arranged by size: 6045 1024 675 375 352 185 177 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8833 PsiI TTA'TAA Cuts at: 6403 6403 Size: 8833 PspOMI G'GGCC_C Cuts at: 3207 3425 4299 5894 3207 Size: 218 874 1595 6146 Fragments arranged by size: 6146 1595 874 218 PspXI vC'TCGA_Gb Cuts at: 3343 3343 Size: 8833 PstI C_TGCA'G Cuts at: 830 3156 4167 4331 4431 4856 6185 830 Size: 2326 1011 164 100 425 1329 3478 Fragments arranged by size: 3478 2326 1329 1011 425 164 100 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8833 PvuII CAG'CTG Cuts at: 2139 2851 4137 4426 5381 5708 2139 Size: 712 1286 289 955 327 5264 Fragments arranged by size: 5264 1286 955 712 327 289 SacI G_AGCT'C Cuts at: 2738 3344 3557 2738 Size: 606 213 8014 Fragments arranged by size: 8014 606 213 SacII CC_GC'GG Cuts at: 2773 5010 2773 Size: 2237 6596 SalI G'TCGA_C Cuts at: 1936 6175 1936 Size: 4239 4594 SapI GCTCTTCn'nnn_ Cuts at: 3299 4644 3299 Size: 1345 7488 SbfI CC_TGCA'GG Cuts at: 4856 6185 4856 Size: 1329 7504 ScaI AGT'ACT Cuts at: 7081 8793 7081 Size: 1712 7121 SexAI A'CCwGG_T Cuts at: 5643 6115 5643 Size: 472 8361 SfcI C'TryA_G Cuts at: 826 1045 3152 3754 4163 4294 4327 4427 4852 Size: 219 2107 602 409 131 33 100 425 Cuts at: 4852 6181 7316 7997 8188 826 Size: 1329 1135 681 191 1471 Fragments arranged by size: 2107 1471 1329 1135 681 602 425 409 219 191 131 100 33 SfoI GGC'GCC Cuts at: 2786 2998 4036 4112 5269 2786 Size: 212 1038 76 1157 6350 Fragments arranged by size: 6350 1157 1038 212 76 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8833 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8833 SmaI CCC'GGG Cuts at: 1872 2084 4304 5829 5875 6160 1872 Size: 212 2220 1525 46 285 4545 Fragments arranged by size: 4545 2220 1525 285 212 46 SmlI C'TyrA_G Cuts at: 820 1017 1943 3343 3547 3998 5147 6937 7838 Size: 197 926 1400 204 451 1149 1790 901 Cuts at: 7838 8100 820 Size: 262 1553 Fragments arranged by size: 1790 1553 1400 1149 926 901 451 262 204 197 SnaBI TAC'GTA Cuts at: 493 6624 493 Size: 6131 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8833 SphI G_CATG'C Cuts at: 1132 2782 3205 4778 6191 1132 Size: 1650 423 1573 1413 3774 Fragments arranged by size: 3774 1650 1573 1413 423 SrfI GCCC'GGGC Cuts at: 5829 5829 Size: 8833 SspI AAT'ATT Cuts at: 5 52 4253 6757 5 Size: 47 4201 2504 2081 Fragments arranged by size: 4201 2504 2081 47 StuI AGG'CCT Cuts at: 1517 2024 3821 4244 4788 4850 1517 Size: 507 1797 423 544 62 5500 Fragments arranged by size: 5500 1797 544 507 423 62 StyI C'CwwG_G Cuts at: 513 1065 2057 3061 3508 5263 5718 6272 513 Size: 552 992 1004 447 1755 455 554 3074 Fragments arranged by size: 3074 1755 1004 992 554 552 455 447 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3173 3323 3374 3575 4416 6860 6877 Size: 1255 1593 150 51 201 841 2444 17 Cuts at: 6877 7030 7215 7660 325 Size: 153 185 445 1498 Fragments arranged by size: 2444 1593 1498 1255 841 445 201 185 153 150 51 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 7079 8791 96 Size: 274 80 33 51 1443 5102 1712 138 Fragments arranged by size: 5102 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3629 3661 8793 8825 3629 Size: 32 5132 32 3637 Fragments arranged by size: 5132 3637 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4052 4172 4382 1316 Size: 2736 120 210 5767 Fragments arranged by size: 5767 2736 210 120 XbaI T'CTAG_A Cuts at: 6169 6169 Size: 8833 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3118 5206 3118 Size: 2088 6745 XhoI C'TCGA_G Cuts at: 1943 3343 1943 Size: 1400 7433 XmaI C'CCGG_G Cuts at: 1870 2082 4302 5827 5873 6158 1870 Size: 212 2220 1525 46 285 4545 Fragments arranged by size: 4545 2220 1525 285 212 46 XmnI GAAnn'nnTTC Cuts at: 4211 6962 4211 Size: 2751 6082 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4384 6641 276 Size: 53 83 186 936 2850 2257 2468 Fragments arranged by size: 2850 2468 2257 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI BaeI BbvCI BsiWI BstXI BstZ17I Bsu36I EcoRV FseI FspAI NruI PacI PsrI RsrII SanDI SfiI SwaI