Restriction map for
pFN21AB5628
(Linear) (Six-base) MAPSORT of: KIBB5628.seq Check: 2106 from: 1 to: 4128
KIBB5628.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AatII G_ACGT'C
Cuts at: 0 2381 4128
Size: 2381 1747
Acc65I G'GTAC_C
Cuts at: 0 4114 4128
Size: 4114 14
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 379 885 2059 2488 4128
Size: 308 71 506 1174 429 1640
Fragments arranged by size:
1640 1174 506 429 308 71
AfeI AGC'GCT
Cuts at: 0 1606 4128
Size: 1606 2522
AflIII A'CryG_T
Cuts at: 0 1013 2402 4128
Size: 1013 1389 1726
Fragments arranged by size:
1726 1389 1013
AgeI A'CCGG_T
Cuts at: 0 3128 4128
Size: 3128 1000
AhdI GACnn_n'nnGTC
Cuts at: 0 2961 3810 4128
Size: 2961 849 318
Fragments arranged by size:
2961 849 318
AleI CACnn'nnGTG
Cuts at: 0 836 2585 3172 4128
Size: 836 1749 587 956
Fragments arranged by size:
1749 956 836 587
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4081 4128
Size: 4081 47
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3674 3706 4128
Size: 3674 32 422
Fragments arranged by size:
3674 422 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1484 2421 3706 4128
Size: 1484 937 1285 422
Fragments arranged by size:
1484 1285 937 422
ApaI G_GGCC'C
Cuts at: 0 1206 1424 2298 3893 4128
Size: 1206 218 874 1595 235
Fragments arranged by size:
1595 1206 874 235 218
ApaLI G'TGCA_C
Cuts at: 0 1086 2516 4128
Size: 1086 1430 1612
Fragments arranged by size:
1612 1430 1086
ApoI r'AATT_y
Cuts at: 0 434 1120 1888 2206 3381 4128
Size: 434 686 768 318 1175 747
Fragments arranged by size:
1175 768 747 686 434 318
AseI AT'TA_AT
Cuts at: 0 247 3545 4128
Size: 247 3298 583
Fragments arranged by size:
3298 583 247
AvaI C'yCGr_G
Cuts at: 0 77 459 1338 2297 2863 3822 3868 4128
Size: 77 382 879 959 566 959 46 260
Fragments arranged by size:
959 959 879 566 382 260 77 46
AvrII C'CTAG_G
Cuts at: 0 1503 4128
Size: 1503 2625
BamHI G'GATC_C
Cuts at: 0 3312 3480 4128
Size: 3312 168 648
Fragments arranged by size:
3312 648 168
BanI G'GyrC_C
Cuts at: 0 117 779 991 1390 1527 1573 2029 2105
Size: 117 662 212 399 137 46 456 76
Cuts at: 2105 2859 3262 3934 4114 4128
Size: 754 403 672 180 14
Fragments arranged by size:
754 672 662 456 403 399 212 180
137 117 76 46 14
BanII G_rGCy'C
Cuts at: 0 154 488 733 1206 1339 1424 1450 1552
Size: 154 334 245 473 133 85 26 102
Cuts at: 1552 1885 2298 2809 3251 3829 3893 4128
Size: 333 413 511 442 578 64 235
Fragments arranged by size:
578 511 473 442 413 334 333 245
235 154 133 102 85 64 26
BbeI G_GCGC'C
Cuts at: 0 783 995 2033 2109 3266 4128
Size: 783 212 1038 76 1157 862
Fragments arranged by size:
1157 1038 862 783 212 76
BbsI GAAGACnn'nnnn_
Cuts at: 0 3641 4128
Size: 3641 487
BfrBI ATG'CAT
Cuts at: 0 2492 4128
Size: 2492 1636
BglI GCCn_nnn'nGGC
Cuts at: 0 1358 2134 4128
Size: 1358 776 1994
Fragments arranged by size:
1994 1358 776
BglII A'GATC_T
Cuts at: 0 2426 3944 4128
Size: 2426 1518 184
Fragments arranged by size:
2426 1518 184
BlpI GC'TnA_GC
Cuts at: 0 130 847 3828 4128
Size: 130 717 2981 300
Fragments arranged by size:
2981 717 300 130
Bme1580I G_kGCm'C
Cuts at: 0 916 1090 1206 1395 1424 1532 1576 2298
Size: 916 174 116 189 29 108 44 722
Cuts at: 2298 2520 3893 3937 4128
Size: 222 1373 44 191
Fragments arranged by size:
1373 916 722 222 191 189 174 116
108 44 44 29
BmgBI CAC'GTC
Cuts at: 0 1931 4128
Size: 1931 2197
BmrI ACTGGGnnnn_n'
Cuts at: 0 1427 3900 4128
Size: 1427 2473 228
Fragments arranged by size:
2473 1427 228
BmtI G_CTAG'C
Cuts at: 0 2371 4128
Size: 2371 1757
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3777 3977 4128
Size: 3777 200 151
Fragments arranged by size:
3777 200 151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 760 884 2568 3375 3683 3819 4104
Size: 200 560 124 1684 807 308 136 285
Cuts at: 4104 4128
Size: 24
Fragments arranged by size:
1684 807 560 308 285 200 136 124
24
Bpu10I CC'TnA_GC
Cuts at: 0 2065 3625 4128
Size: 2065 1560 503
Fragments arranged by size:
2065 1560 503
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1563 2014 3127 4128
Size: 1563 451 1113 1001
Fragments arranged by size:
1563 1113 1001 451
BsaI GGTCTCn'nnnn_
Cuts at: 0 176 4128
Size: 176 3952
BsaAI yAC'GTr
Cuts at: 0 1014 1623 4008 4128
Size: 1014 609 2385 120
Fragments arranged by size:
2385 1014 609 120
BsaHI Gr'CG_yC
Cuts at: 0 293 780 992 1129 1226 2030 2106 2378
Size: 293 487 212 137 97 804 76 272
Cuts at: 2378 2762 3263 4128
Size: 384 501 865
Fragments arranged by size:
865 804 501 487 384 293 272 212
137 97 76
BsaWI w'CCGG_w
Cuts at: 0 1431 2965 3128 4128
Size: 1431 1534 163 1000
Fragments arranged by size:
1534 1431 1000 163
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 691 721 1624 1654 3674 3704 4128
Size: 691 30 903 30 2020 30 424
Fragments arranged by size:
2020 903 691 424 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 997 1000 1309 1560 2133 3319 3577 4128
Size: 997 3 309 251 573 1186 258 551
Fragments arranged by size:
1186 997 573 551 309 258 251 3
BseYI C'CCAG_C
Cuts at: 0 10 1394 1533 2086 2132 2363 2542 2649
Size: 10 1384 139 553 46 231 179 107
Cuts at: 2649 3764 3883 4128
Size: 1115 119 245
Fragments arranged by size:
1384 1115 553 245 231 179 139 119
107 46 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 862 2113 2249 3028 3882 4128
Size: 862 1251 136 779 854 246
Fragments arranged by size:
1251 862 854 779 246 136
BsiEI CG_ry'CG
Cuts at: 0 300 524 887 924 1094 2631 3002 3750
Size: 300 224 363 37 170 1537 371 748
Cuts at: 3750 4128
Size: 378
Fragments arranged by size:
1537 748 378 371 363 300 224 170
37
BsiHKAI G_wGCw'C
Cuts at: 0 131 262 733 853 1090 1339 1410 1552
Size: 131 131 471 120 237 249 71 142
Cuts at: 1552 1628 2520 2716 2744 4007 4128
Size: 76 892 196 28 1263 121
Fragments arranged by size:
1263 892 471 249 237 196 142 131
131 121 120 76 71 28
BsmI GAATG_Cn'
Cuts at: 0 1791 1829 2494 2894 4128
Size: 1791 38 665 400 1234
Fragments arranged by size:
1791 1234 665 400 38
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3921 4128
Size: 3921 207
Bsp1286I G_dGCh'C
Cuts at: 0 131 154 262 488 733 853 916 1090
Size: 131 23 108 226 245 120 63 174
Cuts at: 1090 1206 1339 1395 1410 1424 1450 1532 1552
Size: 116 133 56 15 14 26 82 20
Cuts at: 1552 1576 1628 1885 2298 2520 2716 2744 2809
Size: 24 52 257 413 222 196 28 65
Cuts at: 2809 3251 3829 3893 3937 4007 4128
Size: 442 578 64 44 70 121
Fragments arranged by size:
578 442 413 257 245 226 222 196
174 133 131 121 120 116 108 82
70 65 64 63 56 52 44 28
26 24 23 20 15 14
BspEI T'CCGG_A
Cuts at: 0 1431 2965 4128
Size: 1431 1534 1163
Fragments arranged by size:
1534 1431 1163
BspHI T'CATG_A
Cuts at: 0 140 228 609 645 2438 4128
Size: 140 88 381 36 1793 1690
Fragments arranged by size:
1793 1690 381 140 88 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2526 3086 4128
Size: 2526 560 1042
Fragments arranged by size:
2526 1042 560
BsrBI CCG'CTC
Cuts at: 0 1465 2979 4128
Size: 1465 1514 1149
Fragments arranged by size:
1514 1465 1149
BsrDI GCAATG_nn'
Cuts at: 0 31 1061 2079 4128
Size: 31 1030 1018 2049
Fragments arranged by size:
2049 1030 1018 31
BsrFI r'CCGG_y
Cuts at: 0 88 923 2102 3128 4128
Size: 88 835 1179 1026 1000
Fragments arranged by size:
1179 1026 1000 835 88
BssSI C'ACGA_G
Cuts at: 0 726 2694 4128
Size: 726 1968 1434
Fragments arranged by size:
1968 1434 726
BstAPI GCAn_nnn'nTGC
Cuts at: 0 664 1703 2421 4128
Size: 664 1039 718 1707
Fragments arranged by size:
1707 1039 718 664
BstBI TT'CG_AA
Cuts at: 0 305 1857 4128
Size: 305 1552 2271
Fragments arranged by size:
2271 1552 305
BstEII G'GTnAC_C
Cuts at: 0 813 942 4128
Size: 813 129 3186
Fragments arranged by size:
3186 813 129
BstYI r'GATC_y
Cuts at: 0 505 1049 1733 1954 2426 3111 3312 3480
Size: 505 544 684 221 472 685 201 168
Cuts at: 3480 3944 4128
Size: 464 184
Fragments arranged by size:
685 684 544 505 472 464 221 201
184 168
BtgI C'CryG_G
Cuts at: 0 765 1577 3002 3185 4128
Size: 765 812 1425 183 943
Fragments arranged by size:
1425 943 812 765 183
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 700 4128
Size: 700 3428
BtsI GCAGTG_nn'
Cuts at: 0 1645 1711 2886 3784 4128
Size: 1645 66 1175 898 344
Fragments arranged by size:
1645 1175 898 344 66
DraI TTT'AAA
Cuts at: 0 677 4128
Size: 677 3451
DraIII CAC_nnn'GTG
Cuts at: 0 124 279 4128
Size: 124 155 3849
Fragments arranged by size:
3849 155 124
DrdI GACnn_nn'nnGTC
Cuts at: 0 641 4128
Size: 641 3487
EaeI y'GGCC_r
Cuts at: 0 297 521 872 884 1091 2628 2999 3191
Size: 297 224 351 12 207 1537 371 192
Cuts at: 3191 3710 3747 4010 4128
Size: 519 37 263 118
Fragments arranged by size:
1537 519 371 351 297 263 224 207
192 118 37 12
EagI C'GGCC_G
Cuts at: 0 297 521 884 1091 2628 2999 3747 4128
Size: 297 224 363 207 1537 371 748 381
Fragments arranged by size:
1537 748 381 371 363 297 224 207
EarI CTCTTCn'nnn_
Cuts at: 0 1294 1789 2639 3016 3946 4128
Size: 1294 495 850 377 930 182
Fragments arranged by size:
1294 930 850 495 377 182
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2515 4128
Size: 2515 1613
EcoICRI GAG'CTC
Cuts at: 0 731 1337 1550 4128
Size: 731 606 213 2578
Fragments arranged by size:
2578 731 606 213
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 308 379 760 884 885 2059 2488
Size: 200 108 71 381 124 1 1174 429
Cuts at: 2488 2568 3375 3683 3819 4104 4128
Size: 80 807 308 136 285 24
Fragments arranged by size:
1174 807 429 381 308 285 200 136
124 108 80 71 24 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 976 2158 2839 3320 3925 4128
Size: 976 1182 681 481 605 203
Fragments arranged by size:
1182 976 681 605 481 203
EcoO109I rG'GnC_Cy
Cuts at: 0 1311 1421 1488 2163 2294 2538 2723 3075
Size: 1311 110 67 675 131 244 185 352
Cuts at: 3075 3890 4099 4128
Size: 815 209 29
Fragments arranged by size:
1311 815 675 352 244 209 185 131
110 67 29
EcoRI G'AATT_C
Cuts at: 0 1120 2206 4128
Size: 1120 1086 1922
Fragments arranged by size:
1922 1120 1086
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 915 2958 3114 4128
Size: 915 2043 156 1014
Fragments arranged by size:
2043 1014 915 156
HaeII r_GCGC'y
Cuts at: 0 783 908 995 1608 2033 2109 2154 3266
Size: 783 125 87 613 425 76 45 1112
Cuts at: 3266 3737 3761 4128
Size: 471 24 367
Fragments arranged by size:
1112 783 613 471 425 367 125 87
76 45 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 580 612 1648 1675 1680 1707 1955 1981
Size: 580 32 1036 27 5 27 248 26
Cuts at: 1981 1987 2013 2418 2450 2899 2931 2947 2979
Size: 6 26 405 32 449 32 16 32
Cuts at: 2979 3796 3828 4128
Size: 817 32 300
Fragments arranged by size:
1036 817 580 449 405 300 248 32
32 32 32 32 27 27 26 26
16 6 5
HincII GTy'rAC
Cuts at: 0 2024 2346 4128
Size: 2024 322 1782
Fragments arranged by size:
2024 1782 322
KasI G'GCGC_C
Cuts at: 0 779 991 2029 2105 3262 4128
Size: 779 212 1038 76 1157 866
Fragments arranged by size:
1157 1038 866 779 212 76
KpnI G_GTAC'C
Cuts at: 0 4118 4128
Size: 4118 10
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 522 758 770 2819 4128
Size: 522 236 12 2049 1309
Fragments arranged by size:
2049 1309 522 236 12
MscI TGG'CCA
Cuts at: 0 3193 3712 4128
Size: 3193 519 416
Fragments arranged by size:
3193 519 416
MslI CAynn'nnrTG
Cuts at: 0 233 836 1850 2585 2774 3172 4128
Size: 233 603 1014 735 189 398 956
Fragments arranged by size:
1014 956 735 603 398 233 189
MspA1I CmG'CkG
Cuts at: 0 134 767 846 1271 1782 2132 2421 2478
Size: 134 633 79 425 511 350 289 57
Cuts at: 2478 3004 3291 3376 3703 3887 4128
Size: 526 287 85 327 184 241
Fragments arranged by size:
633 526 511 425 350 327 289 287
241 184 134 85 79 57
NaeI GCC'GGC
Cuts at: 0 90 2104 4128
Size: 90 2014 2024
Fragments arranged by size:
2024 2014 90
NarI GG'CG_CC
Cuts at: 0 780 992 2030 2106 3263 4128
Size: 780 212 1038 76 1157 865
Fragments arranged by size:
1157 1038 865 780 212 76
NgoMIV G'CCGG_C
Cuts at: 0 88 2102 4128
Size: 88 2014 2026
Fragments arranged by size:
2026 2014 88
NheI G'CTAG_C
Cuts at: 0 2367 4128
Size: 2367 1761
NotI GC'GGCC_GC
Cuts at: 0 2999 4128
Size: 2999 1129
NsiI A_TGCA'T
Cuts at: 0 2494 4128
Size: 2494 1634
NspI r_CATG'y
Cuts at: 0 579 777 1200 2406 2773 4128
Size: 579 198 423 1206 367 1355
Fragments arranged by size:
1355 1206 579 423 367 198
PasI CC'CwG_GG
Cuts at: 0 1400 4128
Size: 1400 2728
PciI A'CATG_T
Cuts at: 0 2402 4128
Size: 2402 1726
PflMI CCAn_nnn'nTGG
Cuts at: 0 37 810 3993 4128
Size: 37 773 3183 135
Fragments arranged by size:
3183 773 135 37
PfoI T'CCnGG_A
Cuts at: 0 1628 1807 2522 2903 3791 4128
Size: 1628 179 715 381 888 337
Fragments arranged by size:
1628 888 715 381 337 179
PmlI CAC'GTG
Cuts at: 0 1014 1623 4008 4128
Size: 1014 609 2385 120
Fragments arranged by size:
2385 1014 609 120
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3674 3706 4128
Size: 3674 32 422
Fragments arranged by size:
3674 422 32
PpuMI rG'GwC_Cy
Cuts at: 0 1311 1488 2163 2538 2723 3075 4099 4128
Size: 1311 177 675 375 185 352 1024 29
Fragments arranged by size:
1311 1024 675 375 352 185 177 29
PspOMI G'GGCC_C
Cuts at: 0 1202 1420 2294 3889 4128
Size: 1202 218 874 1595 239
Fragments arranged by size:
1595 1202 874 239 218
PspXI vC'TCGA_Gb
Cuts at: 0 1338 4128
Size: 1338 2790
PstI C_TGCA'G
Cuts at: 0 1151 2162 2326 2426 2851 4128
Size: 1151 1011 164 100 425 1277
Fragments arranged by size:
1277 1151 1011 425 164 100
PvuII CAG'CTG
Cuts at: 0 134 846 2132 2421 3376 3703 4128
Size: 134 712 1286 289 955 327 425
Fragments arranged by size:
1286 955 712 425 327 289 134
SacI G_AGCT'C
Cuts at: 0 733 1339 1552 4128
Size: 733 606 213 2576
Fragments arranged by size:
2576 733 606 213
SacII CC_GC'GG
Cuts at: 0 768 3005 4128
Size: 768 2237 1123
Fragments arranged by size:
2237 1123 768
SapI GCTCTTCn'nnn_
Cuts at: 0 1294 2639 4128
Size: 1294 1345 1489
Fragments arranged by size:
1489 1345 1294
SbfI CC_TGCA'GG
Cuts at: 0 2851 4128
Size: 2851 1277
SexAI A'CCwGG_T
Cuts at: 0 3638 4110 4128
Size: 3638 472 18
Fragments arranged by size:
3638 472 18
SfcI C'TryA_G
Cuts at: 0 1147 1749 2158 2289 2322 2422 2847 4128
Size: 1147 602 409 131 33 100 425 1281
Fragments arranged by size:
1281 1147 602 425 409 131 100 33
SfoI GGC'GCC
Cuts at: 0 781 993 2031 2107 3264 4128
Size: 781 212 1038 76 1157 864
Fragments arranged by size:
1157 1038 864 781 212 76
SmaI CCC'GGG
Cuts at: 0 79 2299 3824 3870 4128
Size: 79 2220 1525 46 258
Fragments arranged by size:
2220 1525 258 79 46
SmlI C'TyrA_G
Cuts at: 0 1338 1542 1993 3142 4128
Size: 1338 204 451 1149 986
Fragments arranged by size:
1338 1149 986 451 204
SphI G_CATG'C
Cuts at: 0 777 1200 2773 4128
Size: 777 423 1573 1355
Fragments arranged by size:
1573 1355 777 423
SrfI GCCC'GGGC
Cuts at: 0 3824 4128
Size: 3824 304
SspI AAT'ATT
Cuts at: 0 2248 4128
Size: 2248 1880
StuI AGG'CCT
Cuts at: 0 19 1816 2239 2783 2845 4128
Size: 19 1797 423 544 62 1283
Fragments arranged by size:
1797 1283 544 423 62 19
StyI C'CwwG_G
Cuts at: 0 52 1056 1503 3258 3713 4128
Size: 52 1004 447 1755 455 415
Fragments arranged by size:
1755 1004 455 447 415 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1168 1318 1369 1570 2411 4128
Size: 1168 150 51 201 841 1717
Fragments arranged by size:
1717 1168 841 201 150 51
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1624 1656 4128
Size: 1624 32 2472
Fragments arranged by size:
2472 1624 32
Tth111I GACn'n_nGTC
Cuts at: 0 2047 2167 2377 4128
Size: 2047 120 210 1751
Fragments arranged by size:
2047 1751 210 120
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1113 3201 4128
Size: 1113 2088 927
Fragments arranged by size:
2088 1113 927
XhoI C'TCGA_G
Cuts at: 0 1338 4128
Size: 1338 2790
XmaI C'CCGG_G
Cuts at: 0 77 2297 3822 3868 4128
Size: 77 2220 1525 46 260
Fragments arranged by size:
2220 1525 260 77 46
XmnI GAAnn'nnTTC
Cuts at: 0 2206 4128
Size: 2206 1922
ZraI GAC'GTC
Cuts at: 0 2379 4128
Size: 2379 1749
Enzymes that do cut:
AatII Acc65I AcuI AfeI AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AvaI
AvrII BamHI BanI BanII BbeI BbsI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI HaeII Hin4I HincII KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmlI PpiI
PpuMI PspOMI PspXI PstI PvuII SacI SacII SapI
SbfI SexAI SfcI SfoI SmaI SmlI SphI SrfI
SspI StuI StyI TaqII TstI Tth111I XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI AclI AflII AscI AsiSI BaeI BbvCI
BcgI BciVI BclI BsaBI BsiWI BsrGI BssHII BstXI
BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspI FspAI
HindIII HpaI MfeI MluI NcoI NdeI NruI PacI
PmeI PshAI PsiI PsrI PvuI RsrII SalI SanDI
ScaI SfiI SgfI SgrAI SnaBI SpeI SwaI TatI
XbaI