Restriction map for
pFN21AB5606
(Circular) (Six-base) MAPSORT of: pFN21AB5606.seq Check: 1203 from: 1 to: 6541
pFN21AB5606 6541 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2665 3235 3515 2665
Size: 570 280 5691
Fragments arranged by size:
5691 570 280
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4351 278
Size: 53 83 186 936 2815 2468
Fragments arranged by size:
2815 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2114 2537 3884 1937
Size: 177 423 1347 4594
Fragments arranged by size:
4594 1347 423 177
Acc65I G'GTAC_C
Cuts at: 3862 3862
Size: 6541
AclI AA'CG_TT
Cuts at: 1563 4668 5041 1563
Size: 3105 373 3063
Fragments arranged by size:
3105 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2831 3756 4604 5988 6256 1632
Size: 1199 925 848 1384 268 1917
Fragments arranged by size:
1917 1384 1199 925 848 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6344
AflIII A'CryG_T
Cuts at: 1203 5440 6417 1203
Size: 4237 977 1327
Fragments arranged by size:
4237 1327 977
AgeI A'CCGG_T
Cuts at: 5372 5372
Size: 6541
AhdI GACnn_n'nnGTC
Cuts at: 5270 5270
Size: 6541
AleI CACnn'nnGTG
Cuts at: 1166 3509 1166
Size: 2343 4198
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6541
AlwNI CAG_nnn'CTG
Cuts at: 2774 3050 3080 4284 5856 2774
Size: 276 30 1204 1572 3459
Fragments arranged by size:
3459 1572 1204 276 30
ApaI G_GGCC'C
Cuts at: 2748 2748
Size: 6541
ApaLI G'TGCA_C
Cuts at: 4598 5754 4598
Size: 1156 5385
ApoI r'AATT_y
Cuts at: 1493 2730 3596 3850 4076 4228 6337 1493
Size: 1237 866 254 226 152 2109 1697
Fragments arranged by size:
2109 1697 1237 866 254 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3414 5095 160
Size: 872 2382 1681 1606
Fragments arranged by size:
2382 1681 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6541
AvaI C'yCGr_G
Cuts at: 1870 1943 3643 3866 1870
Size: 73 1700 223 4545
Fragments arranged by size:
4545 1700 223 73
BamHI G'GATC_C
Cuts at: 3537 3871 4238 3537
Size: 334 367 5840
Fragments arranged by size:
5840 367 334
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3862 5317 618
Size: 325 217 627 2075 1455 1842
Fragments arranged by size:
2075 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2049 2438 2748 3405 3567 3860 1391
Size: 658 389 310 657 162 293 4072
Fragments arranged by size:
4072 658 657 389 310 293 162
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3068 3304 928
Size: 971 1169 236 4165
Fragments arranged by size:
4165 1169 971 236
BbvCI CC'TCA_GC
Cuts at: 2613 2613
Size: 6541
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4732 4766 1903
Size: 34 2795 34 3678
Fragments arranged by size:
3678 2795 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3051 4433 5643 3051
Size: 1382 1210 3949
Fragments arranged by size:
3949 1382 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6541
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5152 136
Size: 107 122 71 631 605 3480 1525
Fragments arranged by size:
3480 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6536 6536
Size: 6541
BlpI GC'TnA_GC
Cuts at: 3958 3958
Size: 6541
Bme1580I G_kGCm'C
Cuts at: 1790 2245 2748 3774 4602 5758 1790
Size: 455 503 1026 828 1156 2573
Fragments arranged by size:
2573 1156 1026 828 503 455
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6541
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2468 3146 4272
Size: 416 53 83 399 24 1051 678 1126
Cuts at: 4272 5230 442
Size: 958 1753
Fragments arranged by size:
1753 1126 1051 958 678 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6541
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2362 2386 2422 2362
Size: 24 36 6481
Fragments arranged by size:
6481 36 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2208 2332 2445 3030 3443 5201 1239
Size: 969 124 113 585 413 1758 2579
Fragments arranged by size:
2579 1758 969 585 413 124 113
Bpu10I CC'TnA_GC
Cuts at: 2613 2740 2613
Size: 127 6414
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2354 2417 3203 4666 5531 5829 2354
Size: 63 786 1463 865 298 3066
Fragments arranged by size:
3066 1463 865 786 298 63
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2069 3731 5204 882
Size: 617 570 1662 1473 2219
Fragments arranged by size:
2219 1662 1473 617 570
BsaAI yAC'GTr
Cuts at: 493 1204 3319 4332 493
Size: 711 2115 1013 2702
Fragments arranged by size:
2702 2115 1013 711
BsaBI GATnn'nnATC
Cuts at: 6535 6535
Size: 6541
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4348 4730 5365 275
Size: 53 83 186 936 2815 382 635 1451
Fragments arranged by size:
2815 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4974 5372 5646 5793 6204 4974
Size: 398 274 147 411 5311
Fragments arranged by size:
5311 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2440 2470 2846 2876 3255
Size: 30 165 30 1037 30 376 30 379
Cuts at: 3255 3285 1178
Size: 30 4434
Fragments arranged by size:
4434 1037 379 376 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2262 2351 2448 2994 3668 1186
Size: 1076 89 97 546 674 4059
Fragments arranged by size:
4059 1076 674 546 97 89
BseYI C'CCAG_C
Cuts at: 2099 2607 2623 2688 2693 5744 6240 2099
Size: 508 16 65 5 3051 496 2400
Fragments arranged by size:
3051 2400 508 496 65 16 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2049 2422 2541 2693 1163
Size: 573 313 373 119 152 5011
Fragments arranged by size:
5011 573 373 313 152 119
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3991 4752 5780 1540
Size: 275 186 1990 761 1028 2301
Fragments arranged by size:
2301 1990 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2438 3189 3256 3833 4602 4687 5758 2438
Size: 751 67 577 769 85 1071 3221
Fragments arranged by size:
3221 1071 769 751 577 85 67
BsmI GAATG_Cn'
Cuts at: 4052 4145 4052
Size: 93 6448
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2245 2438 2748 3189 3256 3405
Size: 399 259 196 193 310 441 67 149
Cuts at: 3405 3567 3774 3833 3860 4602 4687 5758 1391
Size: 162 207 59 27 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 441 399 310 259 207
196 193 162 149 85 67 59 27
BspEI T'CCGG_A
Cuts at: 6204 6204
Size: 6541
BspHI T'CATG_A
Cuts at: 4430 6160 4430
Size: 1730 4811
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2665 3235 3341 3515 3797 3896 844
Size: 1821 570 106 174 282 99 3489
Fragments arranged by size:
3489 1821 570 282 174 106 99
BsrBI CCG'CTC
Cuts at: 1394 1823 3460 4428 1394
Size: 429 1637 968 3507
Fragments arranged by size:
3507 1637 968 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3104 3439 5036 5210 65
Size: 1183 1856 335 1597 174 1396
Fragments arranged by size:
1856 1597 1396 1183 335 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5185 5372 1700
Size: 110 3375 187 2869
Fragments arranged by size:
3375 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6541
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6541
BssSI C'ACGA_G
Cuts at: 4601 5613 4601
Size: 1012 5529
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3342 3789 794
Size: 2548 447 3546
Fragments arranged by size:
3546 2548 447
BstBI TT'CG_AA
Cuts at: 5345 5345
Size: 6541
BstEII G'GTnAC_C
Cuts at: 1184 2966 1184
Size: 1782 4759
BstXI CCAn_nnnn'nTGG
Cuts at: 2090 2386 2581 2979 2090
Size: 296 195 398 5652
Fragments arranged by size:
5652 398 296 195
BstYI r'GATC_y
Cuts at: 1972 3076 3537 3871 4238 4623 4640 6092 6178
Size: 1104 461 334 367 385 17 1452 86
Cuts at: 6178 6190 6536 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1104 461 385 367 346 334
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2637 3300 3605 513
Size: 552 1572 663 305 3449
Fragments arranged by size:
3449 1572 663 552 305
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5802
Fragments arranged by size:
5802 683 56
BtsI GCAGTG_nn'
Cuts at: 2792 3294 3434 3571 3697 4057 4851 4871 2792
Size: 502 140 137 126 360 794 20 4462
Fragments arranged by size:
4462 794 502 360 140 137 126 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6541
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3305 3340 529
Size: 35 2741 35 3730
Fragments arranged by size:
3730 2741 35 35
DraI TTT'AAA
Cuts at: 3845 3845
Size: 6541
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5548 809
Size: 507 4232 1802
Fragments arranged by size:
4232 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2053 3121 3244
Size: 54 1425 50 275 18 223 1068 123
Cuts at: 3244 3988 4877 6506 8
Size: 744 889 1629 43
Fragments arranged by size:
1629 1425 1068 889 744 275 223 123
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3988 1537
Size: 275 2176 4090
Fragments arranged by size:
4090 2176 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2286 4471 1353
Size: 933 2185 3423
Fragments arranged by size:
3423 2185 933
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3155 5128 5502 5648 387
Size: 1064 138 1566 1973 374 146 1280
Fragments arranged by size:
1973 1566 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2436 2436
Size: 6541
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2208 2332 2445 2831 3030 3443 3756
Size: 393 576 124 113 386 199 413 313
Cuts at: 3756 4604 5201 5988 6256 1239
Size: 848 597 787 268 1524
Fragments arranged by size:
1524 848 787 597 576 413 393 386
313 268 199 124 113
EcoNI CCTnn'n_nnAGG
Cuts at: 2721 3012 2721
Size: 291 6250
EcoO109I rG'GnC_Cy
Cuts at: 2744 2745 3140 2744
Size: 1 395 6145
Fragments arranged by size:
6145 395 1
EcoRI G'AATT_C
Cuts at: 3850 4228 3850
Size: 378 6163
FspI TGC'GCA
Cuts at: 5047 5047
Size: 6541
HaeII r_GCGC'y
Cuts at: 1678 2347 5688 1678
Size: 669 3341 2531
Fragments arranged by size:
3341 2531 669
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5182 5214 5256 5288 1373
Size: 32 135 32 3610 32 42 32 2626
Fragments arranged by size:
3610 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2960 3816 3885 4131 669
Size: 988 281 1022 856 69 246 3079
Fragments arranged by size:
3079 1022 988 856 281 246 69
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6541
HpaI GTT'AAC
Cuts at: 4131 4131
Size: 6541
KpnI G_GTAC'C
Cuts at: 3866 3866
Size: 6541
MfeI C'AATT_G
Cuts at: 4140 4140
Size: 6541
MluI A'CGCG_T
Cuts at: 6417 6417
Size: 6541
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5655 5839 1305
Size: 4350 184 2007
Fragments arranged by size:
4350 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3123 10
Size: 54 1425 343 1291 3428
Fragments arranged by size:
3428 1425 1291 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2108 2579 3299 3509 3809 4499 4858
Size: 648 942 471 720 210 300 690 359
Cuts at: 4858 5017 518
Size: 159 2042
Fragments arranged by size:
2042 942 720 690 648 471 359 300
210 159
MspA1I CmG'CkG
Cuts at: 1606 3249 3607 3957 4634 5782 6027 1606
Size: 1643 358 350 677 1148 245 2120
Fragments arranged by size:
2120 1643 1148 677 358 350 245
NcoI C'CATG_G
Cuts at: 513 1065 3300 513
Size: 552 2235 3754
Fragments arranged by size:
3754 2235 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6541
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6541
NotI GC'GGCC_GC
Cuts at: 3988 3988
Size: 6541
NspI r_CATG'y
Cuts at: 1132 1229 3899 5444 1132
Size: 97 2670 1545 2229
Fragments arranged by size:
2670 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2749 1348
Size: 1401 5140
PciI A'CATG_T
Cuts at: 5440 5440
Size: 6541
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2924 3050 3119 1108
Size: 1816 126 69 4530
Fragments arranged by size:
4530 1816 126 69
PfoI T'CCnGG_A
Cuts at: 2317 3007 2317
Size: 690 5851
PmeI GTTT'AAAC
Cuts at: 3845 3845
Size: 6541
PmlI CAC'GTG
Cuts at: 3319 3319
Size: 6541
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4610 4642 6123 6155 4610
Size: 32 1481 32 4996
Fragments arranged by size:
4996 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2474 1613
Size: 861 5680
PsiI TTA'TAA
Cuts at: 4111 4111
Size: 6541
PspOMI G'GGCC_C
Cuts at: 2744 2744
Size: 6541
PstI C_TGCA'G
Cuts at: 830 3893 830
Size: 3063 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6541
SacI G_AGCT'C
Cuts at: 2438 2438
Size: 6541
SacII CC_GC'GG
Cuts at: 3608 3608
Size: 6541
SalI G'TCGA_C
Cuts at: 1936 3883 1936
Size: 1947 4594
SbfI CC_TGCA'GG
Cuts at: 3893 3893
Size: 6541
ScaI AGT'ACT
Cuts at: 4789 6501 4789
Size: 1712 4829
SfcI C'TryA_G
Cuts at: 826 1045 3889 5024 5705 5896 826
Size: 219 2844 1135 681 191 1471
Fragments arranged by size:
2844 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6541
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6541
SmaI CCC'GGG
Cuts at: 1872 3645 3868 1872
Size: 1773 223 4545
Fragments arranged by size:
4545 1773 223
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2333 2396 3182 4645 5546 5808
Size: 197 926 390 63 786 1463 901 262
Cuts at: 5808 820
Size: 1553
Fragments arranged by size:
1553 1463 926 901 786 390 262 197
63
SnaBI TAC'GTA
Cuts at: 493 4332 493
Size: 3839 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6541
SphI G_CATG'C
Cuts at: 1132 3899 1132
Size: 2767 3774
SspI AAT'ATT
Cuts at: 5 52 4465 5
Size: 47 4413 2081
Fragments arranged by size:
4413 2081 47
StuI AGG'CCT
Cuts at: 1517 2659 3485 1517
Size: 1142 826 4573
Fragments arranged by size:
4573 1142 826
StyI C'CwwG_G
Cuts at: 513 1065 3300 3748 3762 3822 3980 513
Size: 552 2235 448 14 60 158 3074
Fragments arranged by size:
3074 2235 552 448 158 60 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2028 2044 2096 2143 2985 4568 4585
Size: 1255 448 16 52 47 842 1583 17
Cuts at: 4585 4738 4923 5368 325
Size: 153 185 445 1498
Fragments arranged by size:
1583 1498 1255 842 448 445 185 153
52 47 17 16
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2166 2305 4787
Size: 274 80 33 51 1443 189 139 2482
Cuts at: 4787 6499 96
Size: 1712 138
Fragments arranged by size:
2482 1712 1443 274 189 139 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6501 6533 6501
Size: 32 6509
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6541
XbaI T'CTAG_A
Cuts at: 2227 3877 2227
Size: 1650 4891
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3029 3451 3029
Size: 422 6119
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6541
XmaI C'CCGG_G
Cuts at: 1870 3643 3866 1870
Size: 1773 223 4545
Fragments arranged by size:
4545 1773 223
XmnI GAAnn'nnTTC
Cuts at: 4670 4670
Size: 6541
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4349 276
Size: 53 83 186 936 2815 2468
Fragments arranged by size:
2815 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PstI PvuI SacI
SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI
PspXI PsrI PvuII RsrII SanDI SapI SexAI SfiI
SfoI SrfI SwaI