Restriction map for
pFN21AB5565
##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI ApaI AscI Asp718I
AspEI AspI AviII AvrII BanI
BanII BbeI BbrPI BfrI BglI
BglII BlnI BsaAI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BspEI BspLU11I BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
Cfr10I CpoI DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I FauI FseI FspI
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MluI MroI MvnI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NspI NspV PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacI SacII
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tth111I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 64 enzymes) #
###################################
AccI 859 GTCGAC GT^[AC][GT]AC
Alw44I 469 GTGCAC G^TGCAC
AlwNI 25 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 469 GTGCAC G^TGCAC
AspHI 469 GTGCAC G[AT]GC[AT]^C
AvaI 93 CTCGAG C^[CT]CG[GA]G
BalI 433 TGGCCA TGG^CCA
BamHI 1461 GGATCC G^GATCC
BbsI 2047 GAAGAC GAAGAC(2/6)
BbvCI 22 CCTCAGC CCTCAGC(-5/-2)
BcgI 861 CGACTTGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1878 CCGGG CC^[GC]GG
BlpI 1048 GCTAAGC GC^T[ACGT]AGC
BmyI 469 GTGCAC G[AGT]GC[ACT]^C
Bpu1102I 1048 GCTAAGC GC^T[ACGT]AGC
BpuAI 2047 GAAGAC GAAGAC(2/6)
BseRI 1244 CTCCTC GAGGAG(10/8)
BsgI 834 CTGCAC GTGCAG(16/14)
BsiHKAI 469 GTGCAC G[AT]GC[AT]^C
BsmAI 2136 GAGAC GTCTC(1/5)
BsmI 334 GAATGC GAATGC(1/-1)
BsoBI 93 CTCGAG C^[CT]CG[GA]G
Bsp1286I 469 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 1511 TGTACA T^GTACA
BspDI 158 ATCGAT AT^CGAT
BsrGI 1511 TGTACA T^GTACA
BstXI 1112 CCAGAAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 1915 GCAGGC GC[ACGT]^[ACGT]GC
CelII 1048 GCTAAGC GC^T[ACGT]AGC
CfoI 40 GCGC GCG^C
ClaI 158 ATCGAT AT^CGAT
DraII 200 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 400 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 433 TGGCCA [CT]^GGCC[GA]
EarI 1985 CTCTTC CTCTTC(1/4)
EcoO109I 200 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 1828 GATATC GAT^ATC
EcoT22I 2125 ATGCAT ATGCA^T
HaeII 39 GGCGCT [GA]GCGC^[CT]
HapII 1878 CCGG C^CGG
HhaI 40 GCGC GCG^C
HinP1I 40 GCGC G^CGC
HpaII 1878 CCGG C^CGG
Ksp632I 1985 CTCTTC CTCTTC(1/4)
MfeI 2085 CAATTG C^AATTG
MluNI 433 TGGCCA TGG^CCA
MscI 433 TGGCCA TGG^CCA
MspA1I 25 CAGCTG C[AC]G^C[GT]G
MspI 1878 CCGG C^CGG
MunI 2085 CAATTG C^AATTG
NciI 1878 CCGGG CC^[GC]GG
NdeI 521 CATATG CA^TATG
NsiI 2125 ATGCAT ATGCA^T
PacI 1396 TTAATTAA TTAAT^TAA
PaeR7I 93 CTCGAG C^TCGAG
PpuMI 200 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 869 CTGCAG CTGCA^G
PvuII 25 CAGCTG CAG^CTG
SalI 859 GTCGAC G^TCGAC
SmlI 93 CTCGAG C^T[CT][GA]AG
SspBI 1511 TGTACA T^GTACA
SspI 179 AATATT AAT^ATT
Tsp45I 2100 GTCAC ^GT[GC]AC
XhoI 93 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 76 GCGG CCGC(-3/-1)
1105 GCGG
BciVI 1306 GGATAC GTATCC(6/5)
459 GTATCC
BclI 303 TGATCA T^GATCA
570 TGATCA
BfaI 448 CTAG C^TAG
1490 CTAG
BsaBI 565 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
877 GATGTCCATC
BsmFI 1372 GTCCC GGGAC(10/14)
1816 GTCCC
BstYI 1461 GGATCC [GA]^GATC[CT]
2205 AGATCC
DraI 690 TTTAAA TTT^AAA
1206 TTTAAA
EcoRI 492 GAATTC G^AATTC
847 GAATTC
EcoT14I 204 CCTTGG C^C[AT][AT]GG
1198 CCAAGG
FbaI 303 TGATCA T^GATCA
570 TGATCA
HaeIII 259 GGCC GG^CC
434 GGCC
MaeI 448 CTAG C^TAG
1490 CTAG
MaeII 890 ACGT A^CGT
2039 ACGT
MamI 565 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
877 GATGTCCATC
MflI 1461 GGATCC [GA]^GATC[CT]
2205 AGATCC
MwoI 21 GCCTCAGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
33 GCTCCTGGCGC
StyI 204 CCTTGG C^C[AT][AT]GG
1198 CCAAGG
XhoII 1461 GGATCC [GA]^GATC[CT]
2205 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AseI AsnI Asp700I AvaII BbvI
BpmI BsaJI BsiYI BslI BspHI
BsrDI BsrI BssKI BstF5I BstNI
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HincII HindII HinfI HphI
ItaI MaeIII MboI MboII MnlI
MseI MslI MvaI NdeII NlaIII
NlaIV PflMI PleI PshBI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I Van91I XcmI
XmnI