(Circular) (Six-base) MAPSORT of: pFN21AB5565.seq Check: 6631 from: 1 to: 6916 pFN21AB5565 6916 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4726 278 Size: 53 83 186 936 3190 2468 Fragments arranged by size: 3190 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2865 4259 1937 Size: 928 1394 4594 Fragments arranged by size: 4594 1394 928 Acc65I G'GTAC_C Cuts at: 4237 4237 Size: 6916 AclI AA'CG_TT Cuts at: 1563 5043 5416 1563 Size: 3480 373 3063 Fragments arranged by size: 3480 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3348 3929 4979 6363 6631 1632 Size: 1716 581 1050 1384 268 1917 Fragments arranged by size: 1917 1716 1384 1050 581 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6719 AflIII A'CryG_T Cuts at: 1203 5815 6792 1203 Size: 4612 977 1327 Fragments arranged by size: 4612 1327 977 AgeI A'CCGG_T Cuts at: 5747 5747 Size: 6916 AhdI GACnn_n'nnGTC Cuts at: 5645 5645 Size: 6916 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6916 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6916 AlwNI CAG_nnn'CTG Cuts at: 2035 4659 6231 2035 Size: 2624 1572 2720 Fragments arranged by size: 2720 2624 1572 ApaLI G'TGCA_C Cuts at: 2474 4973 6129 2474 Size: 2499 1156 3261 Fragments arranged by size: 3261 2499 1156 ApoI r'AATT_y Cuts at: 1493 2387 2430 2497 2655 2699 2852 3216 3536 Size: 894 43 67 158 44 153 364 320 Cuts at: 3536 3952 4076 4225 4451 4603 6712 1493 Size: 416 124 149 226 152 2109 1697 Fragments arranged by size: 2109 1697 894 416 364 320 226 158 153 152 149 124 67 44 43 AseI AT'TA_AT Cuts at: 160 1032 2247 2279 2760 5470 160 Size: 872 1215 32 481 2710 1606 Fragments arranged by size: 2710 1606 1215 872 481 32 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6916 AvaI C'yCGr_G Cuts at: 1870 1943 2098 4241 1870 Size: 73 155 2143 4545 Fragments arranged by size: 4545 2143 155 73 BamHI G'GATC_C Cuts at: 3466 4246 4613 3466 Size: 780 367 5769 Fragments arranged by size: 5769 780 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4237 5692 618 Size: 325 217 627 2450 1455 1842 Fragments arranged by size: 2450 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4235 1391 Size: 2844 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4059 928 Size: 971 2160 3785 Fragments arranged by size: 3785 2160 971 BbvCI CC'TCA_GC Cuts at: 2028 2028 Size: 6916 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2855 2889 5107 5141 1903 Size: 34 918 34 2218 34 3678 Fragments arranged by size: 3678 2218 918 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2475 3305 4808 6018 2475 Size: 830 1503 1210 3373 Fragments arranged by size: 3373 1503 1210 830 BclI T'GATC_A Cuts at: 1548 2308 2575 1548 Size: 760 267 5889 Fragments arranged by size: 5889 760 267 BfrBI ATG'CAT Cuts at: 4132 4132 Size: 6916 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5527 136 Size: 107 122 71 631 605 3855 1525 Fragments arranged by size: 3855 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6911 6911 Size: 6916 BlpI GC'TnA_GC Cuts at: 3054 4333 3054 Size: 1279 5637 Bme1580I G_kGCm'C Cuts at: 1790 2478 4977 6133 1790 Size: 688 2499 1156 2573 Fragments arranged by size: 2573 2499 1156 688 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6916 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4647 5605 442 Size: 416 53 83 399 24 3230 958 1753 Fragments arranged by size: 3230 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6916 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2113 2113 Size: 6916 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2543 3878 4141 5576 1239 Size: 1304 1335 263 1435 2579 Fragments arranged by size: 2579 1435 1335 1304 263 Bpu10I CC'TnA_GC Cuts at: 2028 2028 Size: 6916 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5041 5906 6204 5041 Size: 865 298 5753 Fragments arranged by size: 5753 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5579 882 Size: 617 4080 2219 Fragments arranged by size: 4080 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4707 493 Size: 711 3503 2702 Fragments arranged by size: 3503 2702 711 BsaBI GATnn'nnATC Cuts at: 2574 2886 6910 2574 Size: 312 4024 2580 Fragments arranged by size: 4024 2580 312 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4723 5105 5740 275 Size: 53 83 186 936 3190 382 635 1451 Fragments arranged by size: 3190 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5349 5747 6021 6168 6579 5349 Size: 398 274 147 411 5686 Fragments arranged by size: 5686 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2221 2251 1178 Size: 30 165 30 818 30 5843 Fragments arranged by size: 5843 818 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3240 1186 Size: 2054 4862 BseYI C'CCAG_C Cuts at: 6119 6615 6119 Size: 496 6420 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2824 1163 Size: 573 1088 5255 Fragments arranged by size: 5255 1088 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4366 5127 6155 1540 Size: 275 186 2365 761 1028 2301 Fragments arranged by size: 2365 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2478 4977 5062 6133 2478 Size: 2499 85 1071 3261 Fragments arranged by size: 3261 2499 1071 85 BsmI GAATG_Cn' Cuts at: 2345 4427 4520 2345 Size: 2082 93 4741 Fragments arranged by size: 4741 2082 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2478 4235 4977 5062 6133 1391 Size: 399 688 1757 742 85 1071 2174 Fragments arranged by size: 2174 1757 1071 742 688 399 85 BspEI T'CCGG_A Cuts at: 6579 6579 Size: 6916 BspHI T'CATG_A Cuts at: 3019 3787 4003 4805 6535 3019 Size: 768 216 802 1730 3400 Fragments arranged by size: 3400 1730 802 768 216 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4271 844 Size: 3427 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4803 1394 Size: 429 2980 3507 Fragments arranged by size: 3507 2980 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3243 3891 4029 4191 5411 5585 65 Size: 1183 1995 648 138 162 1220 174 1396 Fragments arranged by size: 1995 1396 1220 1183 648 174 162 138 BsrFI r'CCGG_y Cuts at: 1700 1810 5560 5747 1700 Size: 110 3750 187 2869 Fragments arranged by size: 3750 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3516 96 Size: 3420 3496 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6916 BssSI C'ACGA_G Cuts at: 4976 5988 4976 Size: 1012 5904 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6916 BstBI TT'CG_AA Cuts at: 5720 5720 Size: 6916 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6916 BstXI CCAn_nnnn'nTGG Cuts at: 3124 3124 Size: 6916 BstYI r'GATC_y Cuts at: 1972 3466 4210 4246 4613 4998 5015 6467 6553 Size: 1494 744 36 367 385 17 1452 86 Cuts at: 6553 6565 6911 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1494 1452 744 385 367 346 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5425 Fragments arranged by size: 5425 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6177 Fragments arranged by size: 6177 683 56 BtsI GCAGTG_nn' Cuts at: 3555 4203 4432 5226 5246 3555 Size: 648 229 794 20 5225 Fragments arranged by size: 5225 794 648 229 20 ClaI AT'CG_AT Cuts at: 1554 2164 1554 Size: 610 6306 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6881 DraI TTT'AAA Cuts at: 2697 3213 4220 2697 Size: 516 1007 5393 Fragments arranged by size: 5393 1007 516 DraIII CAC_nnn'GTG Cuts at: 2410 2410 Size: 6916 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5923 809 Size: 507 4607 1802 Fragments arranged by size: 4607 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2438 4363 5252 Size: 54 1425 50 275 18 608 1925 889 Cuts at: 5252 6881 8 Size: 1629 43 Fragments arranged by size: 1925 1629 1425 889 608 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4363 1537 Size: 275 2551 4090 Fragments arranged by size: 4090 2551 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3996 4846 1353 Size: 2643 850 3423 Fragments arranged by size: 3423 2643 850 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5503 5877 6023 387 Size: 1064 138 3914 374 146 1280 Fragments arranged by size: 3914 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2543 3348 3878 3929 4141 4979 5576 Size: 393 911 805 530 51 212 838 597 Cuts at: 5576 6363 6631 1239 Size: 787 268 1524 Fragments arranged by size: 1524 911 838 805 787 597 530 393 268 212 51 EcoO109I rG'GnC_Cy Cuts at: 2206 2206 Size: 6916 EcoRI G'AATT_C Cuts at: 2497 2852 4225 4603 2497 Size: 355 1373 378 4810 Fragments arranged by size: 4810 1373 378 355 EcoRV GAT'ATC Cuts at: 3835 3835 Size: 6916 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3002 3002 Size: 6916 FspI TGC'GCA Cuts at: 5422 5422 Size: 6916 HaeII r_GCGC'y Cuts at: 1678 2048 6063 1678 Size: 370 4015 2531 Fragments arranged by size: 4015 2531 370 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3232 3264 5557 5589 5631 Size: 32 135 32 1660 32 2293 32 42 Cuts at: 5631 5663 1373 Size: 32 2626 Fragments arranged by size: 2626 2293 1660 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2533 2866 2961 4260 4506 669 Size: 988 281 595 333 95 1299 246 3079 Fragments arranged by size: 3079 1299 988 595 333 281 246 95 HindIII A'AGCT_T Cuts at: 748 748 Size: 6916 HpaI GTT'AAC Cuts at: 4506 4506 Size: 6916 KpnI G_GTAC'C Cuts at: 4241 4241 Size: 6916 MfeI C'AATT_G Cuts at: 4090 4515 4090 Size: 425 6491 MluI A'CGCG_T Cuts at: 6792 6792 Size: 6916 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6030 6214 1305 Size: 4725 184 2007 Fragments arranged by size: 4725 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2440 10 Size: 54 1425 343 608 4486 Fragments arranged by size: 4486 1425 608 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3128 4005 4155 4196 4874 5233 5392 Size: 648 1962 877 150 41 678 359 159 Cuts at: 5392 518 Size: 2042 Fragments arranged by size: 2042 1962 877 678 648 359 159 150 41 MspA1I CmG'CkG Cuts at: 1606 2032 4332 5009 6157 6402 1606 Size: 426 2300 677 1148 245 2120 Fragments arranged by size: 2300 2120 1148 677 426 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5425 Fragments arranged by size: 5425 939 552 NdeI CA'TA_TG Cuts at: 387 2527 387 Size: 2140 4776 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6916 NotI GC'GGCC_GC Cuts at: 4363 4363 Size: 6916 NsiI A_TGCA'T Cuts at: 4134 4134 Size: 6916 NspI r_CATG'y Cuts at: 1132 1229 4274 5819 1132 Size: 97 3045 1545 2229 Fragments arranged by size: 3045 2229 1545 97 PacI TTA_AT'TAA Cuts at: 3405 3405 Size: 6916 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6916 PciI A'CATG_T Cuts at: 5815 5815 Size: 6916 PflMI CCAn_nnn'nTGG Cuts at: 1108 2410 2820 3288 1108 Size: 1302 410 468 4736 Fragments arranged by size: 4736 1302 468 410 PfoI T'CCnGG_A Cuts at: 2559 3882 2559 Size: 1323 5593 PmeI GTTT'AAAC Cuts at: 4220 4220 Size: 6916 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4985 5017 6498 6530 4985 Size: 32 1481 32 5371 Fragments arranged by size: 5371 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2206 2206 Size: 6916 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6916 PsiI TTA'TAA Cuts at: 3582 4486 3582 Size: 904 6012 PspXI vC'TCGA_Gb Cuts at: 2098 2098 Size: 6916 PstI C_TGCA'G Cuts at: 830 2878 4268 830 Size: 2048 1390 3478 Fragments arranged by size: 3478 2048 1390 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6916 PvuII CAG'CTG Cuts at: 2032 2032 Size: 6916 SalI G'TCGA_C Cuts at: 1936 2864 4258 1936 Size: 928 1394 4594 Fragments arranged by size: 4594 1394 928 SbfI CC_TGCA'GG Cuts at: 4268 4268 Size: 6916 ScaI AGT'ACT Cuts at: 5164 6876 5164 Size: 1712 5204 SfcI C'TryA_G Cuts at: 826 1045 2133 2679 2874 4264 5399 6080 6271 Size: 219 1088 546 195 1390 1135 681 191 Cuts at: 6271 826 Size: 1471 Fragments arranged by size: 1471 1390 1135 1088 681 546 219 195 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6916 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6916 SmaI CCC'GGG Cuts at: 1872 4243 1872 Size: 2371 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2098 5020 5921 6183 820 Size: 197 926 155 2922 901 262 1553 Fragments arranged by size: 2922 1553 926 901 262 197 155 SnaBI TAC'GTA Cuts at: 493 4707 493 Size: 4214 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6916 SphI G_CATG'C Cuts at: 1132 4274 1132 Size: 3142 3774 SspI AAT'ATT Cuts at: 5 52 2186 4840 5 Size: 47 2134 2654 2081 Fragments arranged by size: 2654 2134 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6916 StyI C'CwwG_G Cuts at: 513 1065 2004 2209 3203 4355 513 Size: 552 939 205 994 1152 3074 Fragments arranged by size: 3074 1152 994 939 552 205 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2066 3494 4943 4960 5113 5298 5743 Size: 1255 486 1428 1449 17 153 185 445 Cuts at: 5743 325 Size: 1498 Fragments arranged by size: 1498 1449 1428 1255 486 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3396 3516 5162 Size: 274 80 33 51 1443 1419 120 1646 Cuts at: 5162 6874 96 Size: 1712 138 Fragments arranged by size: 1712 1646 1443 1419 274 138 120 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2004 2036 2221 2253 3140 3172 6876 6908 2004 Size: 32 185 32 887 32 3704 32 2012 Fragments arranged by size: 3704 2012 887 185 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6916 XbaI T'CTAG_A Cuts at: 4252 4252 Size: 6916 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2669 3699 4116 2669 Size: 1030 417 5469 Fragments arranged by size: 5469 1030 417 XhoI C'TCGA_G Cuts at: 1943 2098 1943 Size: 155 6761 XmaI C'CCGG_G Cuts at: 1870 4241 1870 Size: 2371 4545 XmnI GAAnn'nnTTC Cuts at: 2376 4056 4164 5045 2376 Size: 1680 108 881 4247 Fragments arranged by size: 4247 1680 881 108 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4724 276 Size: 53 83 186 936 3190 2468 Fragments arranged by size: 3190 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PmlI PspOMI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI