Restriction map for
pFN21AB5565
(Linear) (Six-base) MAPSORT of: KIBB5565.seq Check: 2799 from: 1 to: 2211
KIBB5565.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 0 860 2211
Size: 860 1351
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1343 1924 2211
Size: 1343 581 287
Fragments arranged by size:
1343 581 287
AlwNI CAG_nnn'CTG
Cuts at: 0 30 2211
Size: 30 2181
ApaLI G'TGCA_C
Cuts at: 0 469 2211
Size: 469 1742
ApoI r'AATT_y
Cuts at: 0 382 425 492 650 694 847 1211 1531
Size: 382 43 67 158 44 153 364 320
Cuts at: 1531 1947 2071 2211
Size: 416 124 140
Fragments arranged by size:
416 382 364 320 158 153 140 124
67 44 43
AseI AT'TA_AT
Cuts at: 0 242 274 755 2211
Size: 242 32 481 1456
Fragments arranged by size:
1456 481 242 32
AvaI C'yCGr_G
Cuts at: 0 93 2211
Size: 93 2118
BamHI G'GATC_C
Cuts at: 0 1461 2211
Size: 1461 750
BbsI GAAGACnn'nnnn_
Cuts at: 0 2054 2211
Size: 2054 157
BbvCI CC'TCA_GC
Cuts at: 0 23 2211
Size: 23 2188
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 850 884 2211
Size: 850 34 1327
Fragments arranged by size:
1327 850 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 470 1300 2211
Size: 470 830 911
Fragments arranged by size:
911 830 470
BclI T'GATC_A
Cuts at: 0 303 570 2211
Size: 303 267 1641
Fragments arranged by size:
1641 303 267
BfrBI ATG'CAT
Cuts at: 0 2127 2211
Size: 2127 84
BlpI GC'TnA_GC
Cuts at: 0 1049 2211
Size: 1049 1162
Bme1580I G_kGCm'C
Cuts at: 0 473 2211
Size: 473 1738
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 108 2211
Size: 108 2103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 1873 2136 2211
Size: 538 1335 263 75
Fragments arranged by size:
1335 538 263 75
Bpu10I CC'TnA_GC
Cuts at: 0 23 2211
Size: 23 2188
BsaBI GATnn'nnATC
Cuts at: 0 569 881 2211
Size: 569 312 1330
Fragments arranged by size:
1330 569 312
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 216 246 2211
Size: 216 30 1965
Fragments arranged by size:
1965 216 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1235 2211
Size: 1235 976
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 819 2211
Size: 819 1392
BsiHKAI G_wGCw'C
Cuts at: 0 473 2211
Size: 473 1738
BsmI GAATG_Cn'
Cuts at: 0 340 2211
Size: 340 1871
Bsp1286I G_dGCh'C
Cuts at: 0 473 2211
Size: 473 1738
BspHI T'CATG_A
Cuts at: 0 1014 1782 1998 2211
Size: 1014 768 216 213
Fragments arranged by size:
1014 768 216 213
BsrDI GCAATG_nn'
Cuts at: 0 1238 1886 2024 2186 2211
Size: 1238 648 138 162 25
Fragments arranged by size:
1238 648 162 138 25
BsrGI T'GTAC_A
Cuts at: 0 1511 2211
Size: 1511 700
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1119 2211
Size: 1119 1092
BstYI r'GATC_y
Cuts at: 0 1461 2205 2211
Size: 1461 744 6
Fragments arranged by size:
1461 744 6
BtsI GCAGTG_nn'
Cuts at: 0 1550 2198 2211
Size: 1550 648 13
Fragments arranged by size:
1550 648 13
ClaI AT'CG_AT
Cuts at: 0 159 2211
Size: 159 2052
DraI TTT'AAA
Cuts at: 0 692 1208 2211
Size: 692 516 1003
Fragments arranged by size:
1003 692 516
DraIII CAC_nnn'GTG
Cuts at: 0 405 2211
Size: 405 1806
EaeI y'GGCC_r
Cuts at: 0 433 2211
Size: 433 1778
EarI CTCTTCn'nnn_
Cuts at: 0 1991 2211
Size: 1991 220
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 1343 1873 1924 2136 2211
Size: 538 805 530 51 212 75
Fragments arranged by size:
805 538 530 212 75 51
EcoO109I rG'GnC_Cy
Cuts at: 0 201 2211
Size: 201 2010
EcoRI G'AATT_C
Cuts at: 0 492 847 2211
Size: 492 355 1364
Fragments arranged by size:
1364 492 355
EcoRV GAT'ATC
Cuts at: 0 1830 2211
Size: 1830 381
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 997 2211
Size: 997 1214
HaeII r_GCGC'y
Cuts at: 0 43 2211
Size: 43 2168
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1227 1259 2211
Size: 1227 32 952
Fragments arranged by size:
1227 952 32
HincII GTy'rAC
Cuts at: 0 528 861 956 2211
Size: 528 333 95 1255
Fragments arranged by size:
1255 528 333 95
MfeI C'AATT_G
Cuts at: 0 2085 2211
Size: 2085 126
MscI TGG'CCA
Cuts at: 0 435 2211
Size: 435 1776
MslI CAynn'nnrTG
Cuts at: 0 1123 2000 2150 2191 2211
Size: 1123 877 150 41 20
Fragments arranged by size:
1123 877 150 41 20
MspA1I CmG'CkG
Cuts at: 0 27 2211
Size: 27 2184
NdeI CA'TA_TG
Cuts at: 0 522 2211
Size: 522 1689
NsiI A_TGCA'T
Cuts at: 0 2129 2211
Size: 2129 82
PacI TTA_AT'TAA
Cuts at: 0 1400 2211
Size: 1400 811
PflMI CCAn_nnn'nTGG
Cuts at: 0 405 815 1283 2211
Size: 405 410 468 928
Fragments arranged by size:
928 468 410 405
PfoI T'CCnGG_A
Cuts at: 0 554 1877 2211
Size: 554 1323 334
Fragments arranged by size:
1323 554 334
PpuMI rG'GwC_Cy
Cuts at: 0 201 2211
Size: 201 2010
PsiI TTA'TAA
Cuts at: 0 1577 2211
Size: 1577 634
PspXI vC'TCGA_Gb
Cuts at: 0 93 2211
Size: 93 2118
PstI C_TGCA'G
Cuts at: 0 873 2211
Size: 873 1338
PvuII CAG'CTG
Cuts at: 0 27 2211
Size: 27 2184
SalI G'TCGA_C
Cuts at: 0 859 2211
Size: 859 1352
SfcI C'TryA_G
Cuts at: 0 128 674 869 2211
Size: 128 546 195 1342
Fragments arranged by size:
1342 546 195 128
SmlI C'TyrA_G
Cuts at: 0 93 2211
Size: 93 2118
SspI AAT'ATT
Cuts at: 0 181 2211
Size: 181 2030
StyI C'CwwG_G
Cuts at: 0 204 1198 2211
Size: 204 994 1013
Fragments arranged by size:
1013 994 204
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 61 1489 2211
Size: 61 1428 722
Fragments arranged by size:
1428 722 61
TatI w'GTAC_w
Cuts at: 0 1391 1511 2211
Size: 1391 120 700
Fragments arranged by size:
1391 700 120
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 31 216 248 1135 1167 2211
Size: 31 185 32 887 32 1044
Fragments arranged by size:
1044 887 185 32 32 31
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 664 1694 2111 2211
Size: 664 1030 417 100
Fragments arranged by size:
1030 664 417 100
XhoI C'TCGA_G
Cuts at: 0 93 2211
Size: 93 2118
XmnI GAAnn'nnTTC
Cuts at: 0 371 2051 2159 2211
Size: 371 1680 108 52
Fragments arranged by size:
1680 371 108 52
Enzymes that do cut:
AccI AcuI AlwNI ApaLI ApoI AseI AvaI BamHI
BbsI BbvCI BcgI BciVI BclI BfrBI BlpI Bme1580I
BplI BpmI Bpu10I BsaBI BsaXI BseRI BsgI BsiHKAI
BsmI Bsp1286I BspHI BsrDI BsrGI BstXI BstYI BtsI
ClaI DraI DraIII EaeI EarI Eco57MI EcoO109I EcoRI
EcoRV FalI HaeII Hin4I HincII MfeI MscI MslI
MspA1I NdeI NsiI PacI PflMI PfoI PpuMI PsiI
PspXI PstI PvuII SalI SfcI SmlI SspI StyI
TaqII TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI AscI AsiSI AvrII
BaeI BanI BanII BbeI BglI BglII BmgBI BmrI
BmtI BpuEI BsaI BsaAI BsaHI BsaWI BseYI BsiEI
BsiWI BsmBI BspEI BspMI BsrBI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI CspCI
DrdI EagI EciI EcoICRI EcoNI FseI FspI FspAI
HindIII HpaI KasI KpnI MluI MmeI NaeI NarI
NcoI NgoMIV NheI NotI NruI NspI PasI PciI
PmeI PmlI PpiI PshAI PspOMI PsrI PvuI RsrII
SacI SacII SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI SwaI Tth111I XbaI XmaI ZraI