Restriction map for
pFN21AB5551
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# NO CUT ENZYMES ( 91 enzymes) #
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Acc65I AccIII AclI AflII AgeI
Aor51HI AscI AseI AsnI Asp718I
AvrII BamHI BbsI BbvCI BciVI
BfaI BfrI BglI BglII BlnI
BpuAI BsaBI BseAI BsiWI BspDI
BspEI BspHI BspLU11I BsrDI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I ClaI CpoI DraI
DraIII Eco47III EcoO65I EcoRI EcoRV
EcoT22I HindIII HpaI KpnI KspI
MaeI MamI MfeI MluI MroI
MseI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI ScaI SexAI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
SspI StuI SwaI Tru9I XbaI
XhoI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AatII 1036 GACGTC GACGT^C
AccI 1816 GTCTAC GT^[AC][GT]AC
AcsI 176 AAATTC [GA]^AATT[CT]
AflIII 2030 ACGTGT A^C[GA][CT]GT
AhdI 1306 GACTGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1414 GTGCAC G^TGCAC
AlwI 492 GGATC GGATC(4/5)
AocI 349 CCTGAGG CC^T[ACGT]AGG
ApaI 1602 GGGCCC GGGCC^C
ApaLI 1414 GTGCAC G^TGCAC
ApoI 176 AAATTC [GA]^AATT[CT]
AspEI 1306 GACTGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1036 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 595 GGCGCC GGCGC^C
BclI 2 TGATCA T^GATCA
BlpI 1951 GCTGAGC GC^T[ACGT]AGC
BpmI 583 CTGGAG CTGGAG(16/14)
Bpu1102I 1951 GCTGAGC GC^T[ACGT]AGC
BsaWI 875 ACCGGA [AT]^CCGG[AT]
BsgI 1924 GTGCAG GTGCAG(16/14)
BsmBI 1684 GAGACG CGTCTC(1/5)
BsmI 731 GCATTC GAATGC(1/-1)
Bsp1407I 782 TGTACA T^GTACA
BspMI 1829 ACCTGC ACCTGC(4/8)
BsrGI 782 TGTACA T^GTACA
BstAPI 1203 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 492 GGATCT [GA]^GATC[CT]
Bsu36I 349 CCTGAGG CC^T[ACGT]AGG
CelII 1951 GCTGAGC GC^T[ACGT]AGC
DraII 1602 GGGCCCC [GA]G^G[ACGT]CC[CT]
DrdI 1030 GACCTGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1306 GACTGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 349 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1602 GGGCCCC [GA]G^G[ACGT]CC[CT]
FbaI 2 TGATCA T^GATCA
HincII 1000 GTCAAC GT[CT]^[GA]AC
HindII 1000 GTCAAC GT[CT]^[GA]AC
KasI 595 GGCGCC G^GCGCC
MaeIII 865 GTCAC ^GT[ACGT]AC
MflI 492 GGATCT [GA]^GATC[CT]
NarI 595 GGCGCC GG^CGCC
NspI 335 GCATGT [GA]CATG^[CT]
PflFI 1036 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1478 CCACCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1006 CAGCTG CAG^CTG
SacI 1954 GAGCTC GAGCT^C
SapI 807 GAAGAGC GCTCTTC(1/4)
SfoI 595 GGCGCC GGC^GCC
SmaI 1796 CCCGGG CCC^GGG
SspBI 782 TGTACA T^GTACA
Tsp45I 865 GTCAC ^GT[GC]AC
TspRI 714 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 1036 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1478 CCACCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 776 CCAAGGTGTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 492 GGATCT [GA]^GATC[CT]
XmaCI 1796 CCCGGG C^CCGGG
XmaI 1796 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AlwNI 1204 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1825 CAGCACCTG
Asp700I 846 GAATCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1929 GAACACCTTC
AviII 1214 TGCGCA TGC^GCA
1547 TGCGCA
BbrPI 22 CACGTG CAC^GTG
964 CACGTG
BcgI 762 CGACATAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1869 CGAAGGCGGTGC
BsrBI 1710 CCGCTC CCGCTC(-3/-3)
1645 GAGCGG
DsaI 557 CCATGG C^C[GA][CT]GG
1964 CCATGG
EarI 136 CTCTTC CTCTTC(1/4)
807 GAAGAG
EcoNI 300 CCTGTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1059 CCTGGATGAGG
FseI 680 GGCCGGCC GGCCGG^CC
1452 GGCCGGCC
FspI 1214 TGCGCA TGC^GCA
1547 TGCGCA
HaeII 595 GGCGCC [GA]GCGC^[CT]
899 AGCGCC
HgaI 310 GACGC GACGC(5/10)
1686 GACGC
Ksp632I 136 CTCTTC CTCTTC(1/4)
807 GAAGAG
MspA1I 341 CAGCGG C[AC]G^C[GT]G
1006 CAGCTG
NcoI 557 CCATGG C^CATGG
1964 CCATGG
PmaCI 22 CACGTG CAC^GTG
964 CACGTG
PmlI 22 CACGTG CAC^GTG
964 CACGTG
SbfI 1299 CCTGCAGG CCTGCA^GG
1359 CCTGCAGG
Sse8387I 1299 CCTGCAGG CCTGCA^GG
1359 CCTGCAGG
Tsp509I 177 AATT ^AATT
920 AATT
XmnI 846 GAATCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1929 GAACACCTTC
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# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI AvaII BalI BanI
BanII BbvI BcnI BmyI BsaAI
BsaHI BsaI BsaJI BseRI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII EaeI
EagI EclXI Eco52I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeII MboI MboII
MluNI MnlI MscI MslI MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
TseI TthHB8I