(Circular) (Six-base) MAPSORT of: pFN21AB5551.seq Check: 7681 from: 1 to: 6745 pFN21AB5551 6745 bp With 182 enzymes: SgfI * May 22, 2017 10:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3843 3843 Size: 6745 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3045 4555 278 Size: 53 83 186 936 1509 1510 2468 Fragments arranged by size: 2468 1510 1509 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3822 4088 1937 Size: 1885 266 4594 Fragments arranged by size: 4594 1885 266 Acc65I G'GTAC_C Cuts at: 4066 4066 Size: 6745 AclI AA'CG_TT Cuts at: 1563 4872 5245 1563 Size: 3309 373 3063 Fragments arranged by size: 3309 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2128 2831 4808 6192 6460 1632 Size: 496 703 1977 1384 268 1917 Fragments arranged by size: 1977 1917 1384 703 496 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6548 AflIII A'CryG_T Cuts at: 1203 4035 5644 6621 1203 Size: 2832 1609 977 1327 Fragments arranged by size: 2832 1609 1327 977 AgeI A'CCGG_T Cuts at: 5576 5576 Size: 6745 AhdI GACnn_n'nnGTC Cuts at: 3316 5474 3316 Size: 2158 4587 AleI CACnn'nnGTG Cuts at: 1166 3345 3426 3620 1166 Size: 2179 81 194 4291 Fragments arranged by size: 4291 2179 194 81 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2574 3444 1184 Size: 1390 870 4485 Fragments arranged by size: 4485 1390 870 AlwNI CAG_nnn'CTG Cuts at: 3214 3835 4488 6060 3214 Size: 621 653 1572 3899 Fragments arranged by size: 3899 1572 653 621 ApaI G_GGCC'C Cuts at: 3611 3611 Size: 6745 ApaLI G'TGCA_C Cuts at: 3419 4802 5958 3419 Size: 1383 1156 4206 Fragments arranged by size: 4206 1383 1156 ApoI r'AATT_y Cuts at: 1493 2181 4054 4280 4432 6541 1493 Size: 688 1873 226 152 2109 1697 Fragments arranged by size: 2109 1873 1697 688 226 152 AseI AT'TA_AT Cuts at: 160 1032 5299 160 Size: 872 4267 1606 Fragments arranged by size: 4267 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6745 AvaI C'yCGr_G Cuts at: 1870 1943 2168 3801 3962 4070 1870 Size: 73 225 1633 161 108 4545 Fragments arranged by size: 4545 1633 225 161 108 73 BamHI G'GATC_C Cuts at: 4075 4442 4075 Size: 367 6378 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2600 3029 3538 3614 3625 Size: 325 217 627 813 429 509 76 11 Cuts at: 3625 3881 4066 5521 618 Size: 256 185 1455 1842 Fragments arranged by size: 1842 1455 813 627 509 429 325 256 217 185 76 11 BanII G_rGCy'C Cuts at: 1391 3201 3611 3963 4064 1391 Size: 1810 410 352 101 4072 Fragments arranged by size: 4072 1810 410 352 101 BbeI G_GCGC'C Cuts at: 2604 2604 Size: 6745 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5774 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2756 2790 3863 3897 4936 4970 1903 Size: 34 819 34 1073 34 1039 34 3678 Fragments arranged by size: 3678 1073 1039 819 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4637 5847 4637 Size: 1210 5535 BclI T'GATC_A Cuts at: 1548 2007 1548 Size: 459 6286 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5356 136 Size: 107 122 71 631 605 3684 1525 Fragments arranged by size: 3684 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6740 6740 Size: 6745 BlpI GC'TnA_GC Cuts at: 3957 4162 3957 Size: 205 6540 Bme1580I G_kGCm'C Cuts at: 1790 3104 3423 3543 3611 3886 4806 5962 1790 Size: 1314 319 120 68 275 920 1156 2573 Fragments arranged by size: 2573 1314 1156 920 319 275 120 68 BmgBI CAC'GTC Cuts at: 1318 2494 4036 1318 Size: 1176 1542 4027 Fragments arranged by size: 4027 1542 1176 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3556 4476 5434 Size: 416 53 83 399 24 2139 920 958 Cuts at: 5434 442 Size: 1753 Fragments arranged by size: 2139 1753 958 920 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6745 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2609 5405 1239 Size: 1370 2796 2579 Fragments arranged by size: 2796 2579 1370 Bpu10I CC'TnA_GC Cuts at: 2378 3263 2378 Size: 885 5860 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4870 5735 6033 4870 Size: 865 298 5582 Fragments arranged by size: 5582 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2126 2436 3443 5408 882 Size: 617 627 310 1007 1965 2219 Fragments arranged by size: 2219 1965 1007 627 617 310 BsaAI yAC'GTr Cuts at: 493 1204 2029 2575 2971 4536 493 Size: 711 825 546 396 1565 2702 Fragments arranged by size: 2702 1565 825 711 546 396 BsaBI GATnn'nnATC Cuts at: 6739 6739 Size: 6745 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2316 2601 3042 4552 Size: 53 83 186 936 783 285 441 1510 Cuts at: 4552 4934 5569 275 Size: 382 635 1451 Fragments arranged by size: 1510 1451 936 783 635 441 382 285 186 83 53 BsaWI w'CCGG_w Cuts at: 2880 5178 5576 5850 5997 6408 2880 Size: 2298 398 274 147 411 3217 Fragments arranged by size: 3217 2298 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2024 2054 1178 Size: 30 165 30 621 30 5869 Fragments arranged by size: 5869 621 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2188 2303 2372 2666 2741 3086 3223 3226 Size: 1002 115 69 294 75 345 137 3 Cuts at: 3226 3758 1186 Size: 532 4173 Fragments arranged by size: 4173 1002 532 345 294 137 115 75 69 3 BseYI C'CCAG_C Cuts at: 2604 2620 2983 3013 3267 3780 5948 6444 2604 Size: 16 363 30 254 513 2168 496 2905 Fragments arranged by size: 2905 2168 513 496 363 254 30 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3950 1163 Size: 573 2214 3958 Fragments arranged by size: 3958 2214 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2691 3463 3529 4195 4956 5984 Size: 275 186 690 772 66 666 761 1028 Cuts at: 5984 1540 Size: 2301 Fragments arranged by size: 2301 1028 772 761 690 666 275 186 66 BsiHKAI G_wGCw'C Cuts at: 2553 3423 3858 3963 3978 4806 4891 5962 2553 Size: 870 435 105 15 828 85 1071 3336 Fragments arranged by size: 3336 1071 870 828 435 105 85 15 BsmI GAATG_Cn' Cuts at: 2736 4256 4349 2736 Size: 1520 93 5132 Fragments arranged by size: 5132 1520 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3683 3683 Size: 6745 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2553 3104 3201 3423 3543 3611 3858 Size: 399 763 551 97 222 120 68 247 Cuts at: 3858 3886 3963 3978 4064 4806 4891 5962 1391 Size: 28 77 15 86 742 85 1071 2174 Fragments arranged by size: 2174 1071 763 742 551 399 247 222 120 97 86 85 77 68 28 15 BspEI T'CCGG_A Cuts at: 6408 6408 Size: 6745 BspHI T'CATG_A Cuts at: 4634 6364 4634 Size: 1730 5015 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3843 4100 844 Size: 2999 257 3489 Fragments arranged by size: 3489 2999 257 BsrBI CCG'CTC Cuts at: 1394 1823 3652 3717 4632 1394 Size: 429 1829 65 915 3507 Fragments arranged by size: 3507 1829 915 429 65 BsrDI GCAATG_nn' Cuts at: 65 1248 5240 5414 65 Size: 1183 3992 174 1396 Fragments arranged by size: 3992 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2686 2805 3458 5389 5576 1700 Size: 110 876 119 653 1931 187 2869 Fragments arranged by size: 2869 1931 876 653 187 119 110 BsrGI T'GTAC_A Cuts at: 96 2787 96 Size: 2691 4054 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6745 BssSI C'ACGA_G Cuts at: 4805 5817 4805 Size: 1012 5733 BstAPI GCAn_nnn'nTGC Cuts at: 794 3214 794 Size: 2420 4325 BstBI TT'CG_AA Cuts at: 5549 5549 Size: 6745 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6745 BstYI r'GATC_y Cuts at: 1972 2497 4075 4442 4827 4844 6296 6382 6394 Size: 525 1578 367 385 17 1452 86 12 Cuts at: 6394 6740 1972 Size: 346 1977 Fragments arranged by size: 1977 1578 1452 525 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2355 2355 Size: 6745 BtgI C'CryG_G Cuts at: 513 1065 2562 3969 513 Size: 552 1497 1407 3289 Fragments arranged by size: 3289 1497 1407 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3370 487 Size: 683 56 2144 3862 Fragments arranged by size: 3862 2144 683 56 BtsI GCAGTG_nn' Cuts at: 4261 5055 5075 4261 Size: 794 20 5931 Fragments arranged by size: 5931 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6745 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6710 DraI TTT'AAA Cuts at: 4049 4049 Size: 6745 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3041 5752 809 Size: 507 1725 2711 1802 Fragments arranged by size: 2711 1802 1725 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2061 2370 2424 Size: 54 1425 50 275 18 231 309 54 Cuts at: 2424 2684 2688 2778 3297 3460 3480 3526 3918 Size: 260 4 90 519 163 20 46 392 Cuts at: 3918 4192 5081 6710 8 Size: 274 889 1629 43 Fragments arranged by size: 1629 1425 889 519 392 309 275 274 260 231 163 90 54 54 50 46 43 20 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2688 3460 3526 4192 1537 Size: 275 876 772 66 666 4090 Fragments arranged by size: 4090 876 772 666 275 66 EarI CTCTTCn'nnn_ Cuts at: 1353 2147 2807 4675 1353 Size: 794 660 1868 3423 Fragments arranged by size: 3423 1868 794 660 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3557 5332 5706 5852 387 Size: 1064 138 1968 1775 374 146 1280 Fragments arranged by size: 1968 1775 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3961 3961 Size: 6745 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2128 2609 2831 4808 5405 6192 6460 Size: 393 496 481 222 1977 597 787 268 Cuts at: 6460 1239 Size: 1524 Fragments arranged by size: 1977 1524 787 597 496 481 393 268 222 EcoNI CCTnn'n_nnAGG Cuts at: 2309 3068 2309 Size: 759 5986 EcoO109I rG'GnC_Cy Cuts at: 3608 3608 Size: 6745 EcoRI G'AATT_C Cuts at: 4054 4432 4054 Size: 378 6367 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2093 2854 2093 Size: 761 5984 FseI GG_CCGG'CC Cuts at: 2690 3462 2690 Size: 772 5973 FspI TGC'GCA Cuts at: 3221 3554 5251 3221 Size: 333 1697 4715 Fragments arranged by size: 4715 1697 333 HaeII r_GCGC'y Cuts at: 1678 2604 2908 5892 1678 Size: 926 304 2984 2531 Fragments arranged by size: 2984 2531 926 304 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2024 2056 3059 3091 3302 Size: 32 135 32 452 32 1003 32 211 Cuts at: 3302 3334 5386 5418 5460 5492 1373 Size: 32 2052 32 42 32 2626 Fragments arranged by size: 2626 2052 1003 452 211 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3007 4089 4335 669 Size: 988 281 1069 1082 246 3079 Fragments arranged by size: 3079 1082 1069 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6745 HpaI GTT'AAC Cuts at: 4335 4335 Size: 6745 KasI G'GCGC_C Cuts at: 2600 2600 Size: 6745 KpnI G_GTAC'C Cuts at: 4070 4070 Size: 6745 MfeI C'AATT_G Cuts at: 4344 4344 Size: 6745 MluI A'CGCG_T Cuts at: 6621 6621 Size: 6745 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3102 5859 6043 1305 Size: 1797 2757 184 2007 Fragments arranged by size: 2757 2007 1797 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2426 3299 3482 3920 10 Size: 54 1425 343 594 873 183 438 2835 Fragments arranged by size: 2835 1425 873 594 438 343 183 54 MslI CAynn'nnrTG Cuts at: 518 1166 2255 2556 3097 3345 3426 3548 3602 Size: 648 1089 301 541 248 81 122 54 Cuts at: 3602 3620 4703 5062 5221 518 Size: 18 1083 359 159 2042 Fragments arranged by size: 2042 1089 1083 648 541 359 301 248 159 122 81 54 18 MspA1I CmG'CkG Cuts at: 1606 2348 3013 4161 4838 5986 6231 1606 Size: 742 665 1148 677 1148 245 2120 Fragments arranged by size: 2120 1148 1148 742 677 665 245 NaeI GCC'GGC Cuts at: 2688 2807 3460 2688 Size: 119 653 5973 Fragments arranged by size: 5973 653 119 NarI GG'CG_CC Cuts at: 2601 2601 Size: 6745 NcoI C'CATG_G Cuts at: 513 1065 2562 3969 513 Size: 552 1497 1407 3289 Fragments arranged by size: 3289 1497 1407 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6745 NgoMIV G'CCGG_C Cuts at: 2686 2805 3458 2686 Size: 119 653 5973 Fragments arranged by size: 5973 653 119 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6745 NotI GC'GGCC_GC Cuts at: 4192 4192 Size: 6745 NspI r_CATG'y Cuts at: 1132 1229 2344 4103 5648 1132 Size: 97 1115 1759 1545 2229 Fragments arranged by size: 2229 1759 1545 1115 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6745 PciI A'CATG_T Cuts at: 5644 5644 Size: 6745 PflMI CCAn_nnn'nTGG Cuts at: 1108 3489 1108 Size: 2381 4364 PfoI T'CCnGG_A Cuts at: 3645 3645 Size: 6745 PmeI GTTT'AAAC Cuts at: 4049 4049 Size: 6745 PmlI CAC'GTG Cuts at: 2029 2971 2029 Size: 942 5803 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4814 4846 6327 6359 4814 Size: 32 1481 32 5200 Fragments arranged by size: 5200 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6745 PsiI TTA'TAA Cuts at: 4315 4315 Size: 6745 PspOMI G'GGCC_C Cuts at: 3607 3607 Size: 6745 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2528 2560 3926 3958 2528 Size: 32 1366 32 5315 Fragments arranged by size: 5315 1366 32 32 PstI C_TGCA'G Cuts at: 830 2287 2484 2869 3309 3369 4097 830 Size: 1457 197 385 440 60 728 3478 Fragments arranged by size: 3478 1457 728 440 385 197 60 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6745 PvuII CAG'CTG Cuts at: 3013 3013 Size: 6745 SacI G_AGCT'C Cuts at: 3963 3963 Size: 6745 SalI G'TCGA_C Cuts at: 1936 4087 1936 Size: 2151 4594 SapI GCTCTTCn'nnn_ Cuts at: 2807 2807 Size: 6745 SbfI CC_TGCA'GG Cuts at: 3309 3369 4097 3309 Size: 60 728 5957 Fragments arranged by size: 5957 728 60 ScaI AGT'ACT Cuts at: 4993 6705 4993 Size: 1712 5033 SfcI C'TryA_G Cuts at: 826 1045 2283 2480 2865 3305 3365 4093 5228 Size: 219 1238 197 385 440 60 728 1135 Cuts at: 5228 5909 6100 826 Size: 681 191 1471 Fragments arranged by size: 1471 1238 1135 728 681 440 385 219 197 191 60 SfoI GGC'GCC Cuts at: 2602 2602 Size: 6745 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6745 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6745 SmaI CCC'GGG Cuts at: 1872 3803 4072 1872 Size: 1931 269 4545 Fragments arranged by size: 4545 1931 269 SmlI C'TyrA_G Cuts at: 820 1017 1943 4849 5750 6012 820 Size: 197 926 2906 901 262 1553 Fragments arranged by size: 2906 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4536 493 Size: 4043 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6745 SphI G_CATG'C Cuts at: 1132 4103 1132 Size: 2971 3774 SspI AAT'ATT Cuts at: 5 52 4669 5 Size: 47 4617 2081 Fragments arranged by size: 4617 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6745 StyI C'CwwG_G Cuts at: 513 1065 2310 2562 2781 3399 3453 3501 3969 Size: 552 1245 252 219 618 54 48 468 Cuts at: 3969 4014 4184 513 Size: 45 170 3074 Fragments arranged by size: 3074 1245 618 552 468 252 219 170 54 48 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3017 3105 3259 3794 3864 4772 4789 Size: 1255 1437 88 154 535 70 908 17 Cuts at: 4789 4942 5127 5572 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1437 1255 908 535 445 185 154 153 88 70 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2787 3699 4991 Size: 274 80 33 51 1443 810 912 1292 Cuts at: 4991 6703 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1292 912 810 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6705 6737 6705 Size: 32 6713 Tth111I GACn'n_nGTC Cuts at: 1316 3044 1316 Size: 1728 5017 XbaI T'CTAG_A Cuts at: 4081 4081 Size: 6745 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2788 2011 Size: 777 5968 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6745 XmaI C'CCGG_G Cuts at: 1870 3801 4070 1870 Size: 1931 269 4545 Fragments arranged by size: 4545 1931 269 XmnI GAAnn'nnTTC Cuts at: 2855 3938 4874 2855 Size: 1083 936 4726 Fragments arranged by size: 4726 1083 936 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3043 4553 276 Size: 53 83 186 936 1509 1510 2468 Fragments arranged by size: 2468 1510 1509 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoRV FspAI NruI NsiI PacI PpuMI PspXI RsrII SacII SanDI SexAI SfiI SrfI SwaI