Restriction map for
pFN21AB5551
(Linear) (Six-base) MAPSORT of: KIBB5551.seq Check: 8291 from: 1 to: 2040
KIBB5551.orf
With 182 enzymes: SgfI *
May 19, 2017 16:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1838 2040
Size: 1838 202
AatII G_ACGT'C
Cuts at: 0 1040 2040
Size: 1040 1000
AccI GT'mk_AC
Cuts at: 0 1817 2040
Size: 1817 223
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 826 2040
Size: 123 703 1214
Fragments arranged by size:
1214 703 123
AflIII A'CryG_T
Cuts at: 0 2030 2040
Size: 2030 10
AhdI GACnn_n'nnGTC
Cuts at: 0 1311 2040
Size: 1311 729
AleI CACnn'nnGTG
Cuts at: 0 1340 1421 1615 2040
Size: 1340 81 194 425
Fragments arranged by size:
1340 425 194 81
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 569 1439 2040
Size: 569 870 601
Fragments arranged by size:
870 601 569
AlwNI CAG_nnn'CTG
Cuts at: 0 1209 1830 2040
Size: 1209 621 210
Fragments arranged by size:
1209 621 210
ApaI G_GGCC'C
Cuts at: 0 1606 2040
Size: 1606 434
ApaLI G'TGCA_C
Cuts at: 0 1414 2040
Size: 1414 626
ApoI r'AATT_y
Cuts at: 0 176 2040
Size: 176 1864
AvaI C'yCGr_G
Cuts at: 0 163 1796 1957 2040
Size: 163 1633 161 83
Fragments arranged by size:
1633 163 161 83
BanI G'GyrC_C
Cuts at: 0 595 1024 1533 1609 1620 1876 2040
Size: 595 429 509 76 11 256 164
Fragments arranged by size:
595 509 429 256 164 76 11
BanII G_rGCy'C
Cuts at: 0 1196 1606 1958 2040
Size: 1196 410 352 82
Fragments arranged by size:
1196 410 352 82
BbeI G_GCGC'C
Cuts at: 0 599 2040
Size: 599 1441
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 751 785 1858 1892 2040
Size: 751 34 1073 34 148
Fragments arranged by size:
1073 751 148 34 34
BclI T'GATC_A
Cuts at: 0 2 2040
Size: 2 2038
BlpI GC'TnA_GC
Cuts at: 0 1952 2040
Size: 1952 88
Bme1580I G_kGCm'C
Cuts at: 0 1099 1418 1538 1606 1881 2040
Size: 1099 319 120 68 275 159
Fragments arranged by size:
1099 319 275 159 120 68
BmgBI CAC'GTC
Cuts at: 0 489 2031 2040
Size: 489 1542 9
Fragments arranged by size:
1542 489 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 1551 2040
Size: 1551 489
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 2040
Size: 604 1436
Bpu10I CC'TnA_GC
Cuts at: 0 373 1258 2040
Size: 373 885 782
Fragments arranged by size:
885 782 373
BsaI GGTCTCn'nnnn_
Cuts at: 0 121 431 1438 2040
Size: 121 310 1007 602
Fragments arranged by size:
1007 602 310 121
BsaAI yAC'GTr
Cuts at: 0 24 570 966 2040
Size: 24 546 396 1074
Fragments arranged by size:
1074 546 396 24
BsaHI Gr'CG_yC
Cuts at: 0 311 596 1037 2040
Size: 311 285 441 1003
Fragments arranged by size:
1003 441 311 285
BsaWI w'CCGG_w
Cuts at: 0 875 2040
Size: 875 1165
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 19 49 2040
Size: 19 30 1991
Fragments arranged by size:
1991 30 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 183 298 367 661 736 1081 1218 1221
Size: 183 115 69 294 75 345 137 3
Cuts at: 1221 1753 2040
Size: 532 287
Fragments arranged by size:
532 345 294 287 183 137 115 75
69 3
BseYI C'CCAG_C
Cuts at: 0 599 615 978 1008 1262 1775 2040
Size: 599 16 363 30 254 513 265
Fragments arranged by size:
599 513 363 265 254 30 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1945 2040
Size: 1945 95
BsiEI CG_ry'CG
Cuts at: 0 686 1458 1524 2040
Size: 686 772 66 516
Fragments arranged by size:
772 686 516 66
BsiHKAI G_wGCw'C
Cuts at: 0 548 1418 1853 1958 1973 2040
Size: 548 870 435 105 15 67
Fragments arranged by size:
870 548 435 105 67 15
BsmI GAATG_Cn'
Cuts at: 0 731 2040
Size: 731 1309
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1678 2040
Size: 1678 362
Bsp1286I G_dGCh'C
Cuts at: 0 548 1099 1196 1418 1538 1606 1853 1881
Size: 548 551 97 222 120 68 247 28
Cuts at: 1881 1958 1973 2040
Size: 77 15 67
Fragments arranged by size:
551 548 247 222 120 97 77 68
67 28 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1838 2040
Size: 1838 202
BsrBI CCG'CTC
Cuts at: 0 1647 1712 2040
Size: 1647 65 328
Fragments arranged by size:
1647 328 65
BsrFI r'CCGG_y
Cuts at: 0 681 800 1453 2040
Size: 681 119 653 587
Fragments arranged by size:
681 653 587 119
BsrGI T'GTAC_A
Cuts at: 0 782 2040
Size: 782 1258
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1209 2040
Size: 1209 831
BstYI r'GATC_y
Cuts at: 0 492 2040
Size: 492 1548
Bsu36I CC'TnA_GG
Cuts at: 0 350 2040
Size: 350 1690
BtgI C'CryG_G
Cuts at: 0 557 1964 2040
Size: 557 1407 76
Fragments arranged by size:
1407 557 76
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1365 2040
Size: 1365 675
DrdI GACnn_nn'nnGTC
Cuts at: 0 1036 2040
Size: 1036 1004
EaeI y'GGCC_r
Cuts at: 0 56 365 419 679 683 773 1292 1455
Size: 56 309 54 260 4 90 519 163
Cuts at: 1455 1475 1521 1913 2040
Size: 20 46 392 127
Fragments arranged by size:
519 392 309 260 163 127 90 56
54 46 20 4
EagI C'GGCC_G
Cuts at: 0 683 1455 1521 2040
Size: 683 772 66 519
Fragments arranged by size:
772 683 519 66
EarI CTCTTCn'nnn_
Cuts at: 0 142 802 2040
Size: 142 660 1238
Fragments arranged by size:
1238 660 142
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1552 2040
Size: 1552 488
EcoICRI GAG'CTC
Cuts at: 0 1956 2040
Size: 1956 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 604 826 2040
Size: 123 481 222 1214
Fragments arranged by size:
1214 481 222 123
EcoNI CCTnn'n_nnAGG
Cuts at: 0 304 1063 2040
Size: 304 759 977
Fragments arranged by size:
977 759 304
EcoO109I rG'GnC_Cy
Cuts at: 0 1603 2040
Size: 1603 437
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 88 849 2040
Size: 88 761 1191
Fragments arranged by size:
1191 761 88
FseI GG_CCGG'CC
Cuts at: 0 685 1457 2040
Size: 685 772 583
Fragments arranged by size:
772 685 583
FspI TGC'GCA
Cuts at: 0 1216 1549 2040
Size: 1216 333 491
Fragments arranged by size:
1216 491 333
HaeII r_GCGC'y
Cuts at: 0 599 903 2040
Size: 599 304 1137
Fragments arranged by size:
1137 599 304
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 19 51 1054 1086 1297 1329 2040
Size: 19 32 1003 32 211 32 711
Fragments arranged by size:
1003 711 211 32 32 32 19
HincII GTy'rAC
Cuts at: 0 1002 2040
Size: 1002 1038
KasI G'GCGC_C
Cuts at: 0 595 2040
Size: 595 1445
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1097 2040
Size: 1097 943
MscI TGG'CCA
Cuts at: 0 421 1294 1477 1915 2040
Size: 421 873 183 438 125
Fragments arranged by size:
873 438 421 183 125
MslI CAynn'nnrTG
Cuts at: 0 250 551 1092 1340 1421 1543 1597 1615
Size: 250 301 541 248 81 122 54 18
Cuts at: 1615 2040
Size: 425
Fragments arranged by size:
541 425 301 250 248 122 81 54
18
MspA1I CmG'CkG
Cuts at: 0 343 1008 2040
Size: 343 665 1032
Fragments arranged by size:
1032 665 343
NaeI GCC'GGC
Cuts at: 0 683 802 1455 2040
Size: 683 119 653 585
Fragments arranged by size:
683 653 585 119
NarI GG'CG_CC
Cuts at: 0 596 2040
Size: 596 1444
NcoI C'CATG_G
Cuts at: 0 557 1964 2040
Size: 557 1407 76
Fragments arranged by size:
1407 557 76
NgoMIV G'CCGG_C
Cuts at: 0 681 800 1453 2040
Size: 681 119 653 587
Fragments arranged by size:
681 653 587 119
NspI r_CATG'y
Cuts at: 0 339 2040
Size: 339 1701
PflMI CCAn_nnn'nTGG
Cuts at: 0 1484 2040
Size: 1484 556
PfoI T'CCnGG_A
Cuts at: 0 1640 2040
Size: 1640 400
PmlI CAC'GTG
Cuts at: 0 24 966 2040
Size: 24 942 1074
Fragments arranged by size:
1074 942 24
PspOMI G'GGCC_C
Cuts at: 0 1602 2040
Size: 1602 438
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 523 555 1921 1953 2040
Size: 523 32 1366 32 87
Fragments arranged by size:
1366 523 87 32 32
PstI C_TGCA'G
Cuts at: 0 282 479 864 1304 1364 2040
Size: 282 197 385 440 60 676
Fragments arranged by size:
676 440 385 282 197 60
PvuII CAG'CTG
Cuts at: 0 1008 2040
Size: 1008 1032
SacI G_AGCT'C
Cuts at: 0 1958 2040
Size: 1958 82
SapI GCTCTTCn'nnn_
Cuts at: 0 802 2040
Size: 802 1238
SbfI CC_TGCA'GG
Cuts at: 0 1304 1364 2040
Size: 1304 60 676
Fragments arranged by size:
1304 676 60
SfcI C'TryA_G
Cuts at: 0 278 475 860 1300 1360 2040
Size: 278 197 385 440 60 680
Fragments arranged by size:
680 440 385 278 197 60
SfoI GGC'GCC
Cuts at: 0 597 2040
Size: 597 1443
SmaI CCC'GGG
Cuts at: 0 1798 2040
Size: 1798 242
StyI C'CwwG_G
Cuts at: 0 305 557 776 1394 1448 1496 1964 2009
Size: 305 252 219 618 54 48 468 45
Cuts at: 2009 2040
Size: 31
Fragments arranged by size:
618 468 305 252 219 54 48 45
31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1012 1100 1254 1789 1859 2040
Size: 1012 88 154 535 70 181
Fragments arranged by size:
1012 535 181 154 88 70
TatI w'GTAC_w
Cuts at: 0 782 1694 2040
Size: 782 912 346
Fragments arranged by size:
912 782 346
Tth111I GACn'n_nGTC
Cuts at: 0 1039 2040
Size: 1039 1001
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 783 2040
Size: 783 1257
XmaI C'CCGG_G
Cuts at: 0 1796 2040
Size: 1796 244
XmnI GAAnn'nnTTC
Cuts at: 0 850 1933 2040
Size: 850 1083 107
Fragments arranged by size:
1083 850 107
ZraI GAC'GTC
Cuts at: 0 1038 2040
Size: 1038 1002
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AhdI AleI AlfI
AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI
BcgI BclI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI
BsrGI BstAPI BstYI Bsu36I BtgI BtgZI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FseI FspI HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PflMI
PfoI PmlI PspOMI PsrI PstI PvuII SacI SapI
SbfI SfcI SfoI SmaI StyI TaqII TatI Tth111I
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AloI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BciVI BfrBI
BglI BglII BmtI BplI BpuEI BsaBI BsiWI BspEI
BspHI BsrDI BssHII BssSI BstBI BstEII BstXI BstZ17I
BtsI ClaI CspCI DraI DraIII EcoRI EcoRV FspAI
HindIII HpaI KpnI MfeI MluI NdeI NheI NotI
NruI NsiI PacI PasI PciI PmeI PpiI PpuMI
PshAI PsiI PspXI PvuI RsrII SacII SalI SanDI
ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI XbaI XhoI