Restriction map for
pFN21AB5095
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# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI AhdI AocI Aor51HI ApaI
AscI AspEI AvrII BbeI BbrPI
BcgI BfrI BglII BlnI BsaAI
BsaBI BsaHI BsaI BseAI BsiEI
BsiWI BspEI BspMI BsrBI BssHII
BssSI BstBI Bsu36I CpoI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRV FauI FseI
HaeII Hin1I HindIII HpaI KasI
KspI MamI MluI MroI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
StuI SwaI XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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Acc65I 130 GGTACC G^GTACC
AccI 1519 GTATAC GT^[AC][GT]AC
AccII 10 CGCG CG^CG
AciI 9 CCGC CCGC(-3/-1)
Alw44I 1219 GTGCAC G^TGCAC
ApaLI 1219 GTGCAC G^TGCAC
AseI 1842 ATTAAT AT^TAAT
AsnI 1842 ATTAAT AT^TAAT
Asp700I 1881 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 130 GGTACC G^GTACC
AspHI 1219 GTGCAC G[AT]GC[AT]^C
AspI 1594 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2324 TGCGCA TGC^GCA
BalI 2642 TGGCCA TGG^CCA
BanII 783 GAGCCC G[GA]GC[CT]^C
BbvCI 787 CCTCAGC CCTCAGC(-5/-2)
BclI 2567 TGATCA T^GATCA
BglI 2789 GCCGTAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2823 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2823 GCTTAGC GC^T[ACGT]AGC
BsaWI 2625 TCCGGT [AT]^CCGG[AT]
BsiHKAI 1219 GTGCAC G[AT]GC[AT]^C
BsmBI 2710 GAGACG CGTCTC(1/5)
BsmI 2169 GCATTC GAATGC(1/-1)
BspDI 2441 ATCGAT AT^CGAT
BspLU11I 308 ACATGT A^CATGT
BsrDI 2646 CATTGC GCAATG(2/0)
BsrFI 2819 ACCGGC [GA]^CCGG[CT]
Bst1107I 1519 GTATAC GTA^TAC
BstAPI 2541 GCAGAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 10 CGCG CG^CG
BstZ17I 1519 GTATAC GTA^TAC
CelII 2823 GCTTAGC GC^T[ACGT]AGC
Cfr10I 2819 ACCGGC [GA]^CCGG[CT]
ClaI 2441 ATCGAT AT^CGAT
EcoT14I 2769 CCTTGG C^C[AT][AT]GG
FbaI 2567 TGATCA T^GATCA
FspI 2324 TGCGCA TGC^GCA
HgaI 11 GCGTC GACGC(5/10)
KpnI 130 GGTACC GGTAC^C
MfeI 1658 CAATTG C^AATTG
MluNI 2642 TGGCCA TGG^CCA
MscI 2642 TGGCCA TGG^CCA
MunI 1658 CAATTG C^AATTG
MvnI 10 CGCG CG^CG
PflFI 1594 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1918 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1842 ATTAAT AT^TAAT
PstI 1700 CTGCAG CTGCA^G
SpeI 1389 ACTAGT A^CTAGT
SspI 391 AATATT AAT^ATT
StyI 2769 CCTTGG C^C[AT][AT]GG
Tth111I 1594 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1918 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1554 TCTAGA T^CTAGA
XcmI 1596 CCATGTCTGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1881 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AflIII 308 ACATGT A^C[GA][CT]GT
565 ACACGT
BanI 130 GGTACC G^G[CT][GA]CC
1456 GGCACC
BbsI 1666 GAAGAC GAAGAC(2/6)
2016 GTCTTC
BciVI 294 GGATAC GTATCC(6/5)
739 GGATAC
BcnI 428 CCCGG CC^[GC]GG
2106 CCGGG
BmyI 783 GAGCCC G[AGT]GC[ACT]^C
1219 GTGCAC
BpuAI 1666 GAAGAC GAAGAC(2/6)
2016 GTCTTC
BsgI 1328 GTGCAG GTGCAG(16/14)
1969 GTGCAG
Bsp1286I 783 GAGCCC G[AGT]GC[ACT]^C
1219 GTGCAC
BspHI 977 TCATGA T^CATGA
2346 TCATGA
BstEII 841 GGTGACC G^GT[ACGT]ACC
1591 GGTGACC
BstPI 841 GGTGACC G^GT[ACGT]ACC
1591 GGTGACC
CfoI 2325 GCGC GCG^C
2902 GCGC
DraI 91 TTTAAA TTT^AAA
2131 TTTAAA
DraII 1833 AGGCCCT [GA]G^G[ACGT]CC[CT]
2677 AGGGCCT
EaeI 6 TGGCCG [CT]^GGCC[GA]
2642 TGGCCA
EcoO109I 1833 AGGCCCT [GA]G^G[ACGT]CC[CT]
2677 AGGGCCT
EcoO65I 841 GGTGACC G^GT[ACGT]ACC
1591 GGTGACC
EcoRI 648 GAATTC G^AATTC
1370 GAATTC
EcoT22I 685 ATGCAT ATGCA^T
1072 ATGCAT
HhaI 2325 GCGC GCG^C
2902 GCGC
HinP1I 2325 GCGC G^CGC
2902 GCGC
HincII 613 GTTGAC GT[CT]^[GA]AC
1892 GTCAAC
HindII 613 GTTGAC GT[CT]^[GA]AC
1892 GTCAAC
MspA1I 48 CAGCTG C[AC]G^C[GT]G
790 CAGCTG
NciI 428 CCCGG CC^[GC]GG
2106 CCGGG
NsiI 685 ATGCAT ATGCA^T
1072 ATGCAT
PaeR7I 281 CTCGAG C^TCGAG
2707 CTCGAG
PvuII 48 CAGCTG CAG^CTG
790 CAGCTG
RcaI 977 TCATGA T^CATGA
2346 TCATGA
ScaI 1704 AGTACT AGT^ACT
2727 AGTACT
XhoI 281 CTCGAG C^TCGAG
2707 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AvaI AvaII BamHI BbvI
BfaI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1407I
BsrGI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraIII EarI EcoNI EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI SphI
SspBI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII