(Circular) (Six-base) MAPSORT of: pFN21AB5095.seq Check: 2767 from: 1 to: 7627 pFN21AB5095 7627 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5437 278 Size: 53 83 186 936 3901 2468 Fragments arranged by size: 3901 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3525 4970 1937 Size: 1588 1445 4594 Fragments arranged by size: 4594 1588 1445 Acc65I G'GTAC_C Cuts at: 2135 4948 2135 Size: 2813 4814 AclI AA'CG_TT Cuts at: 1563 5754 6127 1563 Size: 4191 373 3063 Fragments arranged by size: 4191 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4008 4389 5690 7074 7342 1632 Size: 2376 381 1301 1384 268 1917 Fragments arranged by size: 2376 1917 1384 1301 381 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7430 AflIII A'CryG_T Cuts at: 1203 2313 2570 6526 7503 1203 Size: 1110 257 3956 977 1327 Fragments arranged by size: 3956 1327 1110 977 257 AgeI A'CCGG_T Cuts at: 6458 6458 Size: 7627 AhdI GACnn_n'nnGTC Cuts at: 6356 6356 Size: 7627 AleI CACnn'nnGTG Cuts at: 1166 2907 3594 3791 3908 1166 Size: 1741 687 197 117 4885 Fragments arranged by size: 4885 1741 687 197 117 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2255 2715 3989 4055 1184 Size: 1071 460 1274 66 4756 Fragments arranged by size: 4756 1274 1071 460 66 AlwNI CAG_nnn'CTG Cuts at: 3929 4570 4700 5370 6942 3929 Size: 641 130 670 1572 4614 Fragments arranged by size: 4614 1572 670 641 130 ApaLI G'TGCA_C Cuts at: 3224 5684 6840 3224 Size: 2460 1156 4011 Fragments arranged by size: 4011 2460 1156 ApoI r'AATT_y Cuts at: 1493 2653 3375 3383 4154 4936 5162 5314 7423 Size: 1160 722 8 771 782 226 152 2109 Cuts at: 7423 1493 Size: 1697 Fragments arranged by size: 2109 1697 1160 782 771 722 226 152 8 AseI AT'TA_AT Cuts at: 160 1032 3848 6181 160 Size: 872 2816 2333 1606 Fragments arranged by size: 2816 2333 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7627 AvaI C'yCGr_G Cuts at: 1870 1943 2286 4588 4712 4952 1870 Size: 73 343 2302 124 240 4545 Fragments arranged by size: 4545 2302 343 240 124 73 BamHI G'GATC_C Cuts at: 3820 3919 4125 4957 5324 3820 Size: 99 206 832 367 6123 Fragments arranged by size: 6123 832 367 206 99 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2135 3461 4948 6403 618 Size: 325 217 627 348 1326 1487 1455 1842 Fragments arranged by size: 1842 1487 1455 1326 627 348 325 217 BanII G_rGCy'C Cuts at: 1391 2792 4946 1391 Size: 1401 2154 4072 Fragments arranged by size: 4072 2154 1401 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3678 4014 928 Size: 971 1779 336 4541 Fragments arranged by size: 4541 1779 971 336 BbvCI CC'TCA_GC Cuts at: 2793 2793 Size: 7627 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5818 5852 1903 Size: 34 3881 34 3678 Fragments arranged by size: 3881 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2293 2738 5519 6729 2293 Size: 445 2781 1210 3191 Fragments arranged by size: 3191 2781 1210 445 BclI T'GATC_A Cuts at: 1548 4572 1548 Size: 3024 4603 BfrBI ATG'CAT Cuts at: 2692 3079 2692 Size: 387 7240 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4800 6238 136 Size: 107 122 71 631 605 3128 1438 1525 Fragments arranged by size: 3128 1525 1438 631 605 122 107 71 BglII A'GATC_T Cuts at: 7622 7622 Size: 7627 BlpI GC'TnA_GC Cuts at: 4829 5044 4829 Size: 215 7412 Bme1580I G_kGCm'C Cuts at: 1790 3228 5688 6844 1790 Size: 1438 2460 1156 2573 Fragments arranged by size: 2573 2460 1438 1156 BmgBI CAC'GTC Cuts at: 1318 2573 2916 3793 1318 Size: 1255 343 877 5152 Fragments arranged by size: 5152 1255 877 343 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4745 5358 6316 Size: 416 53 83 399 24 3328 613 958 Cuts at: 6316 442 Size: 1753 Fragments arranged by size: 3328 1753 958 613 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7627 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2439 4053 4603 2439 Size: 1614 550 5463 Fragments arranged by size: 5463 1614 550 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2991 3540 3698 3951 6287 1239 Size: 1752 549 158 253 2336 2579 Fragments arranged by size: 2579 2336 1752 549 253 158 Bpu10I CC'TnA_GC Cuts at: 2793 2793 Size: 7627 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3427 3775 5752 6617 6915 3427 Size: 348 1977 865 298 4139 Fragments arranged by size: 4139 1977 865 348 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6290 882 Size: 617 4791 2219 Fragments arranged by size: 4791 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5418 493 Size: 711 4214 2702 Fragments arranged by size: 4214 2702 711 BsaBI GATnn'nnATC Cuts at: 7621 7621 Size: 7627 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5434 5816 6451 275 Size: 53 83 186 936 3901 382 635 1451 Fragments arranged by size: 3901 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4630 6060 6458 6732 6879 7290 4630 Size: 1430 398 274 147 411 4967 Fragments arranged by size: 4967 1430 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2631 2661 4584 4614 1178 Size: 30 165 30 1228 30 1923 30 4191 Fragments arranged by size: 4191 1923 1228 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2481 4525 4898 4912 1186 Size: 1295 2044 373 14 3901 Fragments arranged by size: 3901 2044 1295 373 14 BseYI C'CCAG_C Cuts at: 2632 6830 7326 2632 Size: 4198 496 2933 Fragments arranged by size: 4198 2933 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3354 3995 1163 Size: 573 1618 641 4795 Fragments arranged by size: 4795 1618 641 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5077 5838 6866 1540 Size: 275 186 3076 761 1028 2301 Fragments arranged by size: 3076 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3228 5688 5773 6844 3228 Size: 2460 85 1071 4011 Fragments arranged by size: 4011 2460 1071 85 BsmI GAATG_Cn' Cuts at: 4174 5138 5231 4174 Size: 964 93 6570 Fragments arranged by size: 6570 964 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4709 4709 Size: 7627 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2792 3228 4946 5688 5773 6844 1391 Size: 399 1002 436 1718 742 85 1071 2174 Fragments arranged by size: 2174 1718 1071 1002 742 436 399 85 BspEI T'CCGG_A Cuts at: 7290 7290 Size: 7627 BspHI T'CATG_A Cuts at: 2982 4351 5516 7246 2982 Size: 1369 1165 1730 3363 Fragments arranged by size: 3363 1730 1369 1165 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4982 844 Size: 4138 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5514 1394 Size: 429 3691 3507 Fragments arranged by size: 3691 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4650 6122 6296 65 Size: 1183 3402 1472 174 1396 Fragments arranged by size: 3402 1472 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4824 6271 6458 1700 Size: 110 3014 1447 187 2869 Fragments arranged by size: 3014 2869 1447 187 110 BsrGI T'GTAC_A Cuts at: 96 2381 4270 4383 96 Size: 2285 1889 113 3340 Fragments arranged by size: 3340 2285 1889 113 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7627 BssSI C'ACGA_G Cuts at: 5687 6699 5687 Size: 1012 6615 BstAPI GCAn_nnn'nTGC Cuts at: 794 4552 794 Size: 3758 3869 BstBI TT'CG_AA Cuts at: 6431 6431 Size: 7627 BstEII G'GTnAC_C Cuts at: 1184 2846 3596 1184 Size: 1662 750 5215 Fragments arranged by size: 5215 1662 750 BstXI CCAn_nnnn'nTGG Cuts at: 3548 3722 3910 3548 Size: 174 188 7265 Fragments arranged by size: 7265 188 174 BstYI r'GATC_y Cuts at: 1972 3304 3691 3820 3919 4125 4957 5324 5709 Size: 1332 387 129 99 206 832 367 385 Cuts at: 5709 5726 7178 7264 7276 7622 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1332 832 387 385 367 346 206 129 99 86 17 12 BstZ17I GTA'TAC Cuts at: 3526 3526 Size: 7627 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 6136 Fragments arranged by size: 6136 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6888 Fragments arranged by size: 6888 683 56 BtsI GCAGTG_nn' Cuts at: 3883 4239 4849 5143 5937 5957 3883 Size: 356 610 294 794 20 5553 Fragments arranged by size: 5553 794 610 356 294 20 ClaI AT'CG_AT Cuts at: 1554 4447 1554 Size: 2893 4734 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2887 2922 3735 3770 529 Size: 35 2323 35 813 35 4386 Fragments arranged by size: 4386 2323 813 35 35 35 DraI TTT'AAA Cuts at: 2098 4138 4931 2098 Size: 2040 793 4794 Fragments arranged by size: 4794 2040 793 DraIII CAC_nnn'GTG Cuts at: 3455 3989 4008 4413 3455 Size: 534 19 405 6669 Fragments arranged by size: 6669 534 405 19 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6634 809 Size: 507 5318 1802 Fragments arranged by size: 5318 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2011 4647 5074 Size: 54 1425 50 275 18 181 2636 427 Cuts at: 5074 5963 7592 8 Size: 889 1629 43 Fragments arranged by size: 2636 1629 1425 889 427 275 181 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5074 1537 Size: 275 3262 4090 Fragments arranged by size: 4090 3262 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3495 4011 4723 4875 5557 1353 Size: 2142 516 712 152 682 3423 Fragments arranged by size: 3423 2142 712 682 516 152 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6214 6588 6734 387 Size: 1064 138 4625 374 146 1280 Fragments arranged by size: 4625 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2991 3540 3698 3951 4008 4389 5690 Size: 393 1359 549 158 253 57 381 1301 Cuts at: 5690 6287 7074 7342 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1359 1301 787 597 549 393 381 268 253 158 57 EcoNI CCTnn'n_nnAGG Cuts at: 3456 4041 4272 3456 Size: 585 231 6811 Fragments arranged by size: 6811 585 231 EcoO109I rG'GnC_Cy Cuts at: 3839 4683 3839 Size: 844 6783 EcoRI G'AATT_C Cuts at: 2653 3375 4936 5314 2653 Size: 722 1561 378 4966 Fragments arranged by size: 4966 1561 722 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4441 4441 Size: 7627 FspI TGC'GCA Cuts at: 4331 6133 4331 Size: 1802 5825 HaeII r_GCGC'y Cuts at: 1678 6774 1678 Size: 5096 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3602 3634 6268 6300 6342 Size: 32 135 32 2030 32 2634 32 42 Cuts at: 6342 6374 1373 Size: 32 2626 Fragments arranged by size: 2634 2626 2030 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2620 3899 4971 5217 669 Size: 988 281 682 1279 1072 246 3079 Fragments arranged by size: 3079 1279 1072 988 682 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7627 HpaI GTT'AAC Cuts at: 5217 5217 Size: 7627 KpnI G_GTAC'C Cuts at: 2139 4952 2139 Size: 2813 4814 MfeI C'AATT_G Cuts at: 3663 5226 3663 Size: 1563 6064 MluI A'CGCG_T Cuts at: 7503 7503 Size: 7627 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2431 2443 4103 6741 6925 1305 Size: 1126 12 1660 2638 184 2007 Fragments arranged by size: 2638 2007 1660 1126 184 12 MscI TGG'CCA Cuts at: 10 64 1489 1832 4649 10 Size: 54 1425 343 2817 2988 Fragments arranged by size: 2988 2817 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2237 2907 3594 3791 3908 5585 5944 Size: 648 1071 670 687 197 117 1677 359 Cuts at: 5944 6103 518 Size: 159 2042 Fragments arranged by size: 2042 1677 1071 687 670 648 359 197 159 117 MspA1I CmG'CkG Cuts at: 1606 2055 2797 5043 5720 6868 7113 1606 Size: 449 742 2246 677 1148 245 2120 Fragments arranged by size: 2246 2120 1148 742 677 449 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6136 Fragments arranged by size: 6136 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7627 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7627 NotI GC'GGCC_GC Cuts at: 5074 5074 Size: 7627 NsiI A_TGCA'T Cuts at: 2694 3081 2694 Size: 387 7240 NspI r_CATG'y Cuts at: 1132 1229 2194 2236 2317 2602 2696 2972 3868 Size: 97 965 42 81 285 94 276 896 Cuts at: 3868 4985 6530 1132 Size: 1117 1545 2229 Fragments arranged by size: 2229 1545 1117 965 896 285 276 97 94 81 42 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7627 PciI A'CATG_T Cuts at: 2313 6526 2313 Size: 4213 3414 PflMI CCAn_nnn'nTGG Cuts at: 1108 3929 1108 Size: 2821 4806 PfoI T'CCnGG_A Cuts at: 4476 4476 Size: 7627 PmeI GTTT'AAAC Cuts at: 4931 4931 Size: 7627 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4695 4727 5696 5728 7209 7241 4695 Size: 32 969 32 1481 32 5081 Fragments arranged by size: 5081 1481 969 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7627 PsiI TTA'TAA Cuts at: 2676 5197 2676 Size: 2521 5106 PspXI vC'TCGA_Gb Cuts at: 2286 2286 Size: 7627 PstI C_TGCA'G Cuts at: 830 3709 4979 830 Size: 2879 1270 3478 Fragments arranged by size: 3478 2879 1270 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7627 PvuII CAG'CTG Cuts at: 2055 2797 2055 Size: 742 6885 SalI G'TCGA_C Cuts at: 1936 4969 1936 Size: 3033 4594 SbfI CC_TGCA'GG Cuts at: 4979 4979 Size: 7627 ScaI AGT'ACT Cuts at: 3711 4734 5875 7587 3711 Size: 1023 1141 1712 3751 Fragments arranged by size: 3751 1712 1141 1023 SexAI A'CCwGG_T Cuts at: 3267 3451 3576 3267 Size: 184 125 7318 Fragments arranged by size: 7318 184 125 SfcI C'TryA_G Cuts at: 826 1045 2495 2704 3125 3240 3705 4804 4975 Size: 219 1450 209 421 115 465 1099 171 Cuts at: 4975 6110 6791 6982 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1450 1135 1099 681 465 421 219 209 191 171 115 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7627 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7627 SmaI CCC'GGG Cuts at: 1872 4954 1872 Size: 3082 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2286 3406 3754 4712 5731 6632 Size: 197 926 343 1120 348 958 1019 901 Cuts at: 6632 6894 820 Size: 262 1553 Fragments arranged by size: 1553 1120 1019 958 926 901 348 343 262 197 SnaBI TAC'GTA Cuts at: 493 5418 493 Size: 4925 2702 SpeI A'CTAG_T Cuts at: 152 3394 152 Size: 3242 4385 SphI G_CATG'C Cuts at: 1132 2194 2972 3868 4985 1132 Size: 1062 778 896 1117 3774 Fragments arranged by size: 3774 1117 1062 896 778 SspI AAT'ATT Cuts at: 5 52 2398 5551 5 Size: 47 2346 3153 2081 Fragments arranged by size: 3153 2346 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7627 StyI C'CwwG_G Cuts at: 513 1065 2004 4774 5066 513 Size: 552 939 2770 292 3074 Fragments arranged by size: 3074 2770 939 552 292 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5654 5671 5824 6009 6454 325 Size: 1255 4074 17 153 185 445 1498 Fragments arranged by size: 4074 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2381 2491 3709 Size: 274 80 33 51 1443 404 110 1218 Cuts at: 3709 4270 4383 4732 5873 7585 96 Size: 561 113 349 1141 1712 138 Fragments arranged by size: 1712 1443 1218 1141 561 404 349 274 138 113 110 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2631 2663 7587 7619 2631 Size: 32 4924 32 2639 Fragments arranged by size: 4924 2639 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3602 1316 Size: 2286 5341 XbaI T'CTAG_A Cuts at: 3559 4963 3559 Size: 1404 6223 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3608 3608 Size: 7627 XhoI C'TCGA_G Cuts at: 1943 2286 4712 1943 Size: 343 2426 4858 Fragments arranged by size: 4858 2426 343 XmaI C'CCGG_G Cuts at: 1870 4952 1870 Size: 3082 4545 XmnI GAAnn'nnTTC Cuts at: 3890 5756 3890 Size: 1866 5761 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5435 276 Size: 53 83 186 936 3901 2468 Fragments arranged by size: 3901 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI