Restriction map for
pFN21AB5095
(Linear) (Six-base) MAPSORT of: KIBB5095.seq Check: 1586 from: 1 to: 2922
KIBB5095.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 0 1520 2922
Size: 1520 1402
Acc65I G'GTAC_C
Cuts at: 0 130 2922
Size: 130 2792
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2003 2384 2922
Size: 2003 381 538
Fragments arranged by size:
2003 538 381
AflIII A'CryG_T
Cuts at: 0 308 565 2922
Size: 308 257 2357
Fragments arranged by size:
2357 308 257
AleI CACnn'nnGTG
Cuts at: 0 902 1589 1786 1903 2922
Size: 902 687 197 117 1019
Fragments arranged by size:
1019 902 687 197 117
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 250 710 1984 2050 2922
Size: 250 460 1274 66 872
Fragments arranged by size:
1274 872 460 250 66
AlwNI CAG_nnn'CTG
Cuts at: 0 1924 2565 2695 2922
Size: 1924 641 130 227
Fragments arranged by size:
1924 641 227 130
ApaLI G'TGCA_C
Cuts at: 0 1219 2922
Size: 1219 1703
ApoI r'AATT_y
Cuts at: 0 648 1370 1378 2149 2922
Size: 648 722 8 771 773
Fragments arranged by size:
773 771 722 648 8
AseI AT'TA_AT
Cuts at: 0 1843 2922
Size: 1843 1079
AvaI C'yCGr_G
Cuts at: 0 281 2583 2707 2922
Size: 281 2302 124 215
Fragments arranged by size:
2302 281 215 124
BamHI G'GATC_C
Cuts at: 0 1815 1914 2120 2922
Size: 1815 99 206 802
Fragments arranged by size:
1815 802 206 99
BanI G'GyrC_C
Cuts at: 0 130 1456 2922
Size: 130 1326 1466
Fragments arranged by size:
1466 1326 130
BanII G_rGCy'C
Cuts at: 0 787 2922
Size: 787 2135
BbsI GAAGACnn'nnnn_
Cuts at: 0 1673 2009 2922
Size: 1673 336 913
Fragments arranged by size:
1673 913 336
BbvCI CC'TCA_GC
Cuts at: 0 788 2922
Size: 788 2134
BciVI GTATCCnnnnn_n'
Cuts at: 0 288 733 2922
Size: 288 445 2189
Fragments arranged by size:
2189 445 288
BclI T'GATC_A
Cuts at: 0 2567 2922
Size: 2567 355
BfrBI ATG'CAT
Cuts at: 0 687 1074 2922
Size: 687 387 1848
Fragments arranged by size:
1848 687 387
BglI GCCn_nnn'nGGC
Cuts at: 0 2795 2922
Size: 2795 127
BlpI GC'TnA_GC
Cuts at: 0 2824 2922
Size: 2824 98
Bme1580I G_kGCm'C
Cuts at: 0 1223 2922
Size: 1223 1699
BmgBI CAC'GTC
Cuts at: 0 568 911 1788 2922
Size: 568 343 877 1134
Fragments arranged by size:
1134 877 568 343
BmrI ACTGGGnnnn_n'
Cuts at: 0 2740 2922
Size: 2740 182
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 434 2048 2598 2922
Size: 434 1614 550 324
Fragments arranged by size:
1614 550 434 324
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 986 1535 1693 1946 2922
Size: 986 549 158 253 976
Fragments arranged by size:
986 976 549 253 158
Bpu10I CC'TnA_GC
Cuts at: 0 788 2922
Size: 788 2134
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1422 1770 2922
Size: 1422 348 1152
Fragments arranged by size:
1422 1152 348
BsaWI w'CCGG_w
Cuts at: 0 2625 2922
Size: 2625 297
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 626 656 2579 2609 2922
Size: 626 30 1923 30 313
Fragments arranged by size:
1923 626 313 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 476 2520 2893 2907 2922
Size: 476 2044 373 14 15
Fragments arranged by size:
2044 476 373 15 14
BseYI C'CCAG_C
Cuts at: 0 627 2922
Size: 627 2295
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1349 1990 2922
Size: 1349 641 932
Fragments arranged by size:
1349 932 641
BsiHKAI G_wGCw'C
Cuts at: 0 1223 2922
Size: 1223 1699
BsmI GAATG_Cn'
Cuts at: 0 2169 2922
Size: 2169 753
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2704 2922
Size: 2704 218
Bsp1286I G_dGCh'C
Cuts at: 0 787 1223 2922
Size: 787 436 1699
Fragments arranged by size:
1699 787 436
BspHI T'CATG_A
Cuts at: 0 977 2346 2922
Size: 977 1369 576
Fragments arranged by size:
1369 977 576
BsrDI GCAATG_nn'
Cuts at: 0 2645 2922
Size: 2645 277
BsrFI r'CCGG_y
Cuts at: 0 2819 2922
Size: 2819 103
BsrGI T'GTAC_A
Cuts at: 0 376 2265 2378 2922
Size: 376 1889 113 544
Fragments arranged by size:
1889 544 376 113
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2547 2922
Size: 2547 375
BstEII G'GTnAC_C
Cuts at: 0 841 1591 2922
Size: 841 750 1331
Fragments arranged by size:
1331 841 750
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1543 1717 1905 2922
Size: 1543 174 188 1017
Fragments arranged by size:
1543 1017 188 174
BstYI r'GATC_y
Cuts at: 0 1299 1686 1815 1914 2120 2922
Size: 1299 387 129 99 206 802
Fragments arranged by size:
1299 802 387 206 129 99
BstZ17I GTA'TAC
Cuts at: 0 1521 2922
Size: 1521 1401
BtsI GCAGTG_nn'
Cuts at: 0 1878 2234 2844 2922
Size: 1878 356 610 78
Fragments arranged by size:
1878 610 356 78
ClaI AT'CG_AT
Cuts at: 0 2442 2922
Size: 2442 480
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 882 917 1730 1765 2922
Size: 882 35 813 35 1157
Fragments arranged by size:
1157 882 813 35 35
DraI TTT'AAA
Cuts at: 0 93 2133 2922
Size: 93 2040 789
Fragments arranged by size:
2040 789 93
DraIII CAC_nnn'GTG
Cuts at: 0 1450 1984 2003 2408 2922
Size: 1450 534 19 405 514
Fragments arranged by size:
1450 534 514 405 19
EaeI y'GGCC_r
Cuts at: 0 6 2642 2922
Size: 6 2636 280
Fragments arranged by size:
2636 280 6
EarI CTCTTCn'nnn_
Cuts at: 0 1490 2006 2718 2870 2922
Size: 1490 516 712 152 52
Fragments arranged by size:
1490 712 516 152 52
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 986 1535 1693 1946 2003 2384 2922
Size: 986 549 158 253 57 381 538
Fragments arranged by size:
986 549 538 381 253 158 57
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1451 2036 2267 2922
Size: 1451 585 231 655
Fragments arranged by size:
1451 655 585 231
EcoO109I rG'GnC_Cy
Cuts at: 0 1834 2678 2922
Size: 1834 844 244
Fragments arranged by size:
1834 844 244
EcoRI G'AATT_C
Cuts at: 0 648 1370 2922
Size: 648 722 1552
Fragments arranged by size:
1552 722 648
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2436 2922
Size: 2436 486
FspI TGC'GCA
Cuts at: 0 2326 2922
Size: 2326 596
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1597 1629 2922
Size: 1597 32 1293
Fragments arranged by size:
1597 1293 32
HincII GTy'rAC
Cuts at: 0 615 1894 2922
Size: 615 1279 1028
Fragments arranged by size:
1279 1028 615
KpnI G_GTAC'C
Cuts at: 0 134 2922
Size: 134 2788
MfeI C'AATT_G
Cuts at: 0 1658 2922
Size: 1658 1264
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 426 438 2098 2922
Size: 426 12 1660 824
Fragments arranged by size:
1660 824 426 12
MscI TGG'CCA
Cuts at: 0 2644 2922
Size: 2644 278
MslI CAynn'nnrTG
Cuts at: 0 232 902 1589 1786 1903 2922
Size: 232 670 687 197 117 1019
Fragments arranged by size:
1019 687 670 232 197 117
MspA1I CmG'CkG
Cuts at: 0 50 792 2922
Size: 50 742 2130
Fragments arranged by size:
2130 742 50
NsiI A_TGCA'T
Cuts at: 0 689 1076 2922
Size: 689 387 1846
Fragments arranged by size:
1846 689 387
NspI r_CATG'y
Cuts at: 0 189 231 312 597 691 967 1863 2922
Size: 189 42 81 285 94 276 896 1059
Fragments arranged by size:
1059 896 285 276 189 94 81 42
PciI A'CATG_T
Cuts at: 0 308 2922
Size: 308 2614
PflMI CCAn_nnn'nTGG
Cuts at: 0 1924 2922
Size: 1924 998
PfoI T'CCnGG_A
Cuts at: 0 2471 2922
Size: 2471 451
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2690 2722 2922
Size: 2690 32 200
Fragments arranged by size:
2690 200 32
PsiI TTA'TAA
Cuts at: 0 671 2922
Size: 671 2251
PspXI vC'TCGA_Gb
Cuts at: 0 281 2922
Size: 281 2641
PstI C_TGCA'G
Cuts at: 0 1704 2922
Size: 1704 1218
PvuII CAG'CTG
Cuts at: 0 50 792 2922
Size: 50 742 2130
Fragments arranged by size:
2130 742 50
ScaI AGT'ACT
Cuts at: 0 1706 2729 2922
Size: 1706 1023 193
Fragments arranged by size:
1706 1023 193
SexAI A'CCwGG_T
Cuts at: 0 1262 1446 1571 2922
Size: 1262 184 125 1351
Fragments arranged by size:
1351 1262 184 125
SfcI C'TryA_G
Cuts at: 0 490 699 1120 1235 1700 2799 2922
Size: 490 209 421 115 465 1099 123
Fragments arranged by size:
1099 490 465 421 209 123 115
SmlI C'TyrA_G
Cuts at: 0 281 1401 1749 2707 2922
Size: 281 1120 348 958 215
Fragments arranged by size:
1120 958 348 281 215
SpeI A'CTAG_T
Cuts at: 0 1389 2922
Size: 1389 1533
SphI G_CATG'C
Cuts at: 0 189 967 1863 2922
Size: 189 778 896 1059
Fragments arranged by size:
1059 896 778 189
SspI AAT'ATT
Cuts at: 0 393 2922
Size: 393 2529
StyI C'CwwG_G
Cuts at: 0 2769 2922
Size: 2769 153
TatI w'GTAC_w
Cuts at: 0 376 486 1704 2265 2378 2727 2922
Size: 376 110 1218 561 113 349 195
Fragments arranged by size:
1218 561 376 349 195 113 110
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 626 658 2922
Size: 626 32 2264
Fragments arranged by size:
2264 626 32
Tth111I GACn'n_nGTC
Cuts at: 0 1597 2922
Size: 1597 1325
XbaI T'CTAG_A
Cuts at: 0 1554 2922
Size: 1554 1368
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1603 2922
Size: 1603 1319
XhoI C'TCGA_G
Cuts at: 0 281 2707 2922
Size: 281 2426 215
Fragments arranged by size:
2426 281 215
XmnI GAAnn'nnTTC
Cuts at: 0 1885 2922
Size: 1885 1037
Enzymes that do cut:
AccI Acc65I AcuI AflIII AleI AlfI AlwNI ApaLI
ApoI AseI AvaI BamHI BanI BanII BbsI BbvCI
BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrDI BsrFI
BsrGI BstAPI BstEII BstXI BstYI BstZ17I BtsI ClaI
CspCI DraI DraIII EaeI EarI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI Hin4I HincII KpnI MfeI MmeI
MscI MslI MspA1I NsiI NspI PciI PflMI PfoI
PpiI PsiI PspXI PstI PvuII ScaI SexAI SfcI
SmlI SpeI SphI SspI StyI TatI TstI Tth111I
XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AloI
ApaI AscI AsiSI AvrII BaeI BbeI BcgI BglII
BmtI BsaI BsaAI BsaBI BsaHI BsiEI BsiWI BspEI
BspMI BsrBI BssHII BssSI BstBI Bsu36I BtgI BtgZI
DrdI EagI EciI EcoICRI EcoRV FseI FspAI HaeII
HindIII HpaI KasI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI PacI PasI PmeI PmlI
PpuMI PshAI PspOMI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI
SmaI SnaBI SrfI StuI SwaI TaqII XmaI ZraI