(Circular) (Six-base) MAPSORT of: pFN21AB3947.seq Check: 6152 from: 1 to: 7369 pFN21AB3947 7369 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5179 278 Size: 53 83 186 936 3643 2468 Fragments arranged by size: 3643 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3112 3349 3895 4712 1937 Size: 1175 237 546 817 4594 Fragments arranged by size: 4594 1175 817 546 237 Acc65I G'GTAC_C Cuts at: 2549 4690 2549 Size: 2141 5228 AclI AA'CG_TT Cuts at: 1563 5496 5869 1563 Size: 3933 373 3063 Fragments arranged by size: 3933 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3861 5432 6816 7084 1632 Size: 2229 1571 1384 268 1917 Fragments arranged by size: 2229 1917 1571 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7172 AflIII A'CryG_T Cuts at: 1203 2109 6268 7245 1203 Size: 906 4159 977 1327 Fragments arranged by size: 4159 1327 977 906 AgeI A'CCGG_T Cuts at: 6200 6200 Size: 7369 AhdI GACnn_n'nnGTC Cuts at: 4589 6098 4589 Size: 1509 5860 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7369 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7369 AlwNI CAG_nnn'CTG Cuts at: 2820 5112 6684 2820 Size: 2292 1572 3505 Fragments arranged by size: 3505 2292 1572 ApaLI G'TGCA_C Cuts at: 5426 6582 5426 Size: 1156 6213 ApoI r'AATT_y Cuts at: 1493 2248 3383 3653 4210 4678 4904 5056 7165 Size: 755 1135 270 557 468 226 152 2109 Cuts at: 7165 1493 Size: 1697 Fragments arranged by size: 2109 1697 1135 755 557 468 270 226 152 AseI AT'TA_AT Cuts at: 160 1032 5923 160 Size: 872 4891 1606 Fragments arranged by size: 4891 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7369 AvaI C'yCGr_G Cuts at: 1870 1943 2673 2721 4694 1870 Size: 73 730 48 1973 4545 Fragments arranged by size: 4545 1973 730 73 48 BamHI G'GATC_C Cuts at: 4699 5066 4699 Size: 367 7002 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2549 3737 4690 6145 618 Size: 325 217 627 762 1188 953 1455 1842 Fragments arranged by size: 1842 1455 1188 953 762 627 325 217 BanII G_rGCy'C Cuts at: 1391 4223 4688 1391 Size: 2832 465 4072 Fragments arranged by size: 4072 2832 465 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3782 4667 928 Size: 971 1883 885 3630 Fragments arranged by size: 3630 1883 971 885 BbvCI CC'TCA_GC Cuts at: 4443 4443 Size: 7369 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5560 5594 1903 Size: 34 3623 34 3678 Fragments arranged by size: 3678 3623 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5261 6471 5261 Size: 1210 6159 BclI T'GATC_A Cuts at: 1548 2949 1548 Size: 1401 5968 BfrBI ATG'CAT Cuts at: 2029 2214 2029 Size: 185 7184 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5980 136 Size: 107 122 71 631 605 4308 1525 Fragments arranged by size: 4308 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2130 7364 2130 Size: 5234 2135 BlpI GC'TnA_GC Cuts at: 2708 3423 4786 2708 Size: 715 1363 5291 Fragments arranged by size: 5291 1363 715 Bme1580I G_kGCm'C Cuts at: 1790 5430 6586 1790 Size: 3640 1156 2573 Fragments arranged by size: 3640 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3116 3893 1318 Size: 1798 777 4794 Fragments arranged by size: 4794 1798 777 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3773 5100 6058 Size: 416 53 83 399 24 2356 1327 958 Cuts at: 6058 442 Size: 1753 Fragments arranged by size: 2356 1753 1327 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7369 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3515 6029 1239 Size: 2276 2514 2579 Fragments arranged by size: 2579 2514 2276 Bpu10I CC'TnA_GC Cuts at: 4443 4443 Size: 7369 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2293 5494 6359 6657 2293 Size: 3201 865 298 3005 Fragments arranged by size: 3201 3005 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6032 882 Size: 617 4533 2219 Fragments arranged by size: 4533 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3797 5160 493 Size: 711 2593 1363 2702 Fragments arranged by size: 2702 2593 1363 711 BsaBI GATnn'nnATC Cuts at: 7363 7363 Size: 7369 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5176 5558 6193 275 Size: 53 83 186 936 3643 382 635 1451 Fragments arranged by size: 3643 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5802 6200 6474 6621 7032 5802 Size: 398 274 147 411 6139 Fragments arranged by size: 6139 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4623 4653 1178 Size: 30 165 30 3220 30 3894 Fragments arranged by size: 3894 3220 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2829 3253 1186 Size: 1643 424 5302 Fragments arranged by size: 5302 1643 424 BseYI C'CCAG_C Cuts at: 2641 3433 4232 4547 6572 7068 2641 Size: 792 799 315 2025 496 2942 Fragments arranged by size: 2942 2025 799 792 496 315 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4372 1163 Size: 573 2636 4160 Fragments arranged by size: 4160 2636 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4819 5580 6608 1540 Size: 275 186 2818 761 1028 2301 Fragments arranged by size: 2818 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5430 5515 6586 5430 Size: 85 1071 6213 Fragments arranged by size: 6213 1071 85 BsmI GAATG_Cn' Cuts at: 2031 3370 4880 4973 2031 Size: 1339 1510 93 4427 Fragments arranged by size: 4427 1510 1339 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4223 4688 5430 5515 6586 1391 Size: 399 2433 465 742 85 1071 2174 Fragments arranged by size: 2433 2174 1071 742 465 399 85 BspEI T'CCGG_A Cuts at: 7032 7032 Size: 7369 BspHI T'CATG_A Cuts at: 2628 3466 5258 6988 2628 Size: 838 1792 1730 3009 Fragments arranged by size: 3009 1792 1730 838 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4090 4724 844 Size: 3246 634 3489 Fragments arranged by size: 3489 3246 634 BsrBI CCG'CTC Cuts at: 1394 1823 5256 1394 Size: 429 3433 3507 Fragments arranged by size: 3507 3433 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4287 5864 6038 65 Size: 1183 3039 1577 174 1396 Fragments arranged by size: 3039 1577 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6013 6200 1700 Size: 110 4203 187 2869 Fragments arranged by size: 4203 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7369 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7369 BssSI C'ACGA_G Cuts at: 2478 3771 4019 5429 6441 2478 Size: 1293 248 1410 1012 3406 Fragments arranged by size: 3406 1410 1293 1012 248 BstAPI GCAn_nnn'nTGC Cuts at: 794 2432 794 Size: 1638 5731 BstBI TT'CG_AA Cuts at: 2023 6173 2023 Size: 4150 3219 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7369 BstYI r'GATC_y Cuts at: 1972 2130 4699 5066 5451 5468 6920 7006 7018 Size: 158 2569 367 385 17 1452 86 12 Cuts at: 7018 7364 1972 Size: 346 1977 Fragments arranged by size: 2569 1977 1452 385 367 346 158 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3966 4227 513 Size: 552 2901 261 3655 Fragments arranged by size: 3655 2901 552 261 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6630 Fragments arranged by size: 6630 683 56 BtsI GCAGTG_nn' Cuts at: 2188 3858 4885 5679 5699 2188 Size: 1670 1027 794 20 3858 Fragments arranged by size: 3858 1670 1027 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7369 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7334 DraI TTT'AAA Cuts at: 4673 4673 Size: 7369 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6376 809 Size: 507 5060 1802 Fragments arranged by size: 5060 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3693 4816 5705 Size: 54 1425 50 275 18 1863 1123 889 Cuts at: 5705 7334 8 Size: 1629 43 Fragments arranged by size: 1863 1629 1425 1123 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4816 1537 Size: 275 3004 4090 Fragments arranged by size: 4090 3004 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2160 3159 4102 5299 1353 Size: 807 999 943 1197 3423 Fragments arranged by size: 3423 1197 999 943 807 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5956 6330 6476 387 Size: 1064 138 4367 374 146 1280 Fragments arranged by size: 4367 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3515 3861 5432 6029 6816 7084 1239 Size: 393 1883 346 1571 597 787 268 1524 Fragments arranged by size: 1883 1571 1524 787 597 393 346 268 EcoO109I rG'GnC_Cy Cuts at: 2498 3629 2498 Size: 1131 6238 EcoRI G'AATT_C Cuts at: 4678 5056 4678 Size: 378 6991 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3031 3704 3031 Size: 673 6696 FspI TGC'GCA Cuts at: 5875 5875 Size: 7369 HaeII r_GCGC'y Cuts at: 1678 6516 1678 Size: 4838 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2770 2802 2815 2847 3023 Size: 32 135 32 1198 32 13 32 176 Cuts at: 3023 3055 3266 3298 3448 3480 4575 4607 6010 Size: 32 211 32 150 32 1095 32 1403 Cuts at: 6010 6042 6084 6116 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1403 1198 1095 211 176 150 135 42 32 32 32 32 32 32 32 32 32 32 13 HincII GTy'rAC Cuts at: 669 1657 1938 2258 4198 4713 4959 669 Size: 988 281 320 1940 515 246 3079 Fragments arranged by size: 3079 1940 988 515 320 281 246 HindIII A'AGCT_T Cuts at: 748 2373 3509 748 Size: 1625 1136 4608 Fragments arranged by size: 4608 1625 1136 HpaI GTT'AAC Cuts at: 4959 4959 Size: 7369 KpnI G_GTAC'C Cuts at: 2553 4694 2553 Size: 2141 5228 MfeI C'AATT_G Cuts at: 3103 4968 3103 Size: 1865 5504 MluI A'CGCG_T Cuts at: 7245 7245 Size: 7369 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2120 2469 3426 3598 6483 6667 1305 Size: 815 349 957 172 2885 184 2007 Fragments arranged by size: 2885 2007 957 815 349 184 172 MscI TGG'CCA Cuts at: 10 64 1489 1832 3695 10 Size: 54 1425 343 1863 3684 Fragments arranged by size: 3684 1863 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2093 2108 3471 3971 5327 5686 5845 Size: 648 927 15 1363 500 1356 359 159 Cuts at: 5845 518 Size: 2042 Fragments arranged by size: 2042 1363 1356 927 648 500 359 159 15 MspA1I CmG'CkG Cuts at: 1606 4386 4785 5462 6610 6855 1606 Size: 2780 399 677 1148 245 2120 Fragments arranged by size: 2780 2120 1148 677 399 245 NcoI C'CATG_G Cuts at: 513 1065 3966 4227 513 Size: 552 2901 261 3655 Fragments arranged by size: 3655 2901 552 261 NdeI CA'TA_TG Cuts at: 387 387 Size: 7369 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7369 NotI GC'GGCC_GC Cuts at: 4816 4816 Size: 7369 NsiI A_TGCA'T Cuts at: 2031 2216 2031 Size: 185 7184 NspI r_CATG'y Cuts at: 1132 1229 2113 2602 4485 4727 6272 1132 Size: 97 884 489 1883 242 1545 2229 Fragments arranged by size: 2229 1883 1545 884 489 242 97 PasI CC'CwG_GG Cuts at: 1348 2055 3321 1348 Size: 707 1266 5396 Fragments arranged by size: 5396 1266 707 PciI A'CATG_T Cuts at: 2109 6268 2109 Size: 4159 3210 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7369 PmeI GTTT'AAAC Cuts at: 4673 4673 Size: 7369 PmlI CAC'GTG Cuts at: 3797 3797 Size: 7369 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5438 5470 6951 6983 5438 Size: 32 1481 32 5824 Fragments arranged by size: 5824 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2498 3629 2498 Size: 1131 6238 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7369 PsiI TTA'TAA Cuts at: 2192 3203 3555 4939 2192 Size: 1011 352 1384 4622 Fragments arranged by size: 4622 1384 1011 352 PstI C_TGCA'G Cuts at: 830 3716 3814 4721 830 Size: 2886 98 907 3478 Fragments arranged by size: 3478 2886 907 98 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7369 PvuII CAG'CTG Cuts at: 4386 4386 Size: 7369 SalI G'TCGA_C Cuts at: 1936 4711 1936 Size: 2775 4594 SbfI CC_TGCA'GG Cuts at: 4721 4721 Size: 7369 ScaI AGT'ACT Cuts at: 3288 5617 7329 3288 Size: 2329 1712 3328 Fragments arranged by size: 3328 2329 1712 SfcI C'TryA_G Cuts at: 826 1045 3075 3350 3712 3810 3896 4401 4717 Size: 219 2030 275 362 98 86 505 316 Cuts at: 4717 5852 6533 6724 826 Size: 1135 681 191 1471 Fragments arranged by size: 2030 1471 1135 681 505 362 316 275 219 191 98 86 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7369 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7369 SmaI CCC'GGG Cuts at: 1872 4696 1872 Size: 2824 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2308 5473 6374 6636 820 Size: 197 926 365 3165 901 262 1553 Fragments arranged by size: 3165 1553 926 901 365 262 197 SnaBI TAC'GTA Cuts at: 493 5160 493 Size: 4667 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7369 SphI G_CATG'C Cuts at: 1132 4485 4727 1132 Size: 3353 242 3774 Fragments arranged by size: 3774 3353 242 SspI AAT'ATT Cuts at: 5 52 2473 5293 5 Size: 47 2421 2820 2081 Fragments arranged by size: 2820 2421 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7369 StyI C'CwwG_G Cuts at: 513 1065 2655 3564 3966 4146 4227 4642 4808 Size: 552 1590 909 402 180 81 415 166 Cuts at: 4808 513 Size: 3074 Fragments arranged by size: 3074 1590 909 552 415 402 180 166 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4409 5396 5413 5566 5751 6196 325 Size: 1255 2829 987 17 153 185 445 1498 Fragments arranged by size: 2829 1498 1255 987 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2324 2849 3009 Size: 274 80 33 51 1443 347 525 160 Cuts at: 3009 3286 4067 5615 7327 96 Size: 277 781 1548 1712 138 Fragments arranged by size: 1712 1548 1443 781 525 347 277 274 160 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3757 3789 7329 7361 3757 Size: 32 3540 32 3765 Fragments arranged by size: 3765 3540 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7369 XbaI T'CTAG_A Cuts at: 4705 4705 Size: 7369 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7369 XmaI C'CCGG_G Cuts at: 1870 4694 1870 Size: 2824 4545 XmnI GAAnn'nnTTC Cuts at: 5498 5498 Size: 7369 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5177 276 Size: 53 83 186 936 3643 2468 Fragments arranged by size: 3643 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI