Restriction map for
pFN21AB3701
(Circular) (Six-base) MAPSORT of: pFN21AB3701.seq Check: 6223 from: 1 to: 8239
pFN21AB3701 8239 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2983 2983
Size: 8239
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3846 6049 278
Size: 53 83 186 936 2310 2203 2468
Fragments arranged by size:
2468 2310 2203 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3030 4989 5582 1937
Size: 1093 1959 593 4594
Fragments arranged by size:
4594 1959 1093 593
Acc65I G'GTAC_C
Cuts at: 5560 5560
Size: 8239
AclI AA'CG_TT
Cuts at: 1563 6366 6739 1563
Size: 4803 373 3063
Fragments arranged by size:
4803 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2030 3697 3781 3793 4265 4928 6302 7686
Size: 398 1667 84 12 472 663 1374 1384
Cuts at: 7686 7954 1632
Size: 268 1917
Fragments arranged by size:
1917 1667 1384 1374 663 472 398 268
84 12
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8042
AflIII A'CryG_T
Cuts at: 1203 4326 4584 7138 8115 1203
Size: 3123 258 2554 977 1327
Fragments arranged by size:
3123 2554 1327 977 258
AgeI A'CCGG_T
Cuts at: 7070 7070
Size: 8239
AhdI GACnn_n'nnGTC
Cuts at: 6968 6968
Size: 8239
AleI CACnn'nnGTG
Cuts at: 1166 3746 4478 1166
Size: 2580 732 4927
Fragments arranged by size:
4927 2580 732
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5192 1184
Size: 4008 4231
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3425 3457 3425
Size: 32 8207
AlwNI CAG_nnn'CTG
Cuts at: 3418 3428 3733 3928 4705 4972 5413 5982 7554
Size: 10 305 195 777 267 441 569 1572
Cuts at: 7554 3418
Size: 4103
Fragments arranged by size:
4103 1572 777 569 441 305 267 195
10
ApaI G_GGCC'C
Cuts at: 3343 3496 4441 3343
Size: 153 945 7141
Fragments arranged by size:
7141 945 153
ApaLI G'TGCA_C
Cuts at: 2345 2624 6296 7452 2345
Size: 279 3672 1156 3132
Fragments arranged by size:
3672 3132 1156 279
ApoI r'AATT_y
Cuts at: 1493 5548 5774 5926 8035 1493
Size: 4055 226 152 2109 1697
Fragments arranged by size:
4055 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6793 160
Size: 872 5761 1606
Fragments arranged by size:
5761 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8239
AvaI C'yCGr_G
Cuts at: 1870 1943 2469 2658 3666 3685 4527 5564 1870
Size: 73 526 189 1008 19 842 1037 4545
Fragments arranged by size:
4545 1037 1008 842 526 189 73 19
BamHI G'GATC_C
Cuts at: 5569 5936 5569
Size: 367 7872
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2094 2814 2927 4205 4480
Size: 325 217 627 307 720 113 1278 275
Cuts at: 4480 5344 5560 7015 618
Size: 864 216 1455 1842
Fragments arranged by size:
1842 1455 1278 864 720 627 325 307
275 217 216 113
BanII G_rGCy'C
Cuts at: 1391 3343 3496 3894 3903 4441 4528 5244 5558
Size: 1952 153 398 9 538 87 716 314
Cuts at: 5558 1391
Size: 4072
Fragments arranged by size:
4072 1952 716 538 398 314 153 87
9
BbeI G_GCGC'C
Cuts at: 2098 5348 2098
Size: 3250 4989
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3377 3973 4015 5224 928
Size: 971 1478 596 42 1209 3943
Fragments arranged by size:
3943 1478 1209 971 596 42
BbvCI CC'TCA_GC
Cuts at: 3060 3060
Size: 8239
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5036 5070 6430 6464 1903
Size: 34 50 34 3015 34 1360 34 3678
Fragments arranged by size:
3678 3015 1360 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6131 7341 6131
Size: 1210 7029
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8239
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4355 4445 5396
Size: 107 122 71 631 605 2683 90 951
Cuts at: 5396 6850 136
Size: 1454 1525
Fragments arranged by size:
2683 1525 1454 951 631 605 122 107
90 71
BglII A'GATC_T
Cuts at: 2748 8234 2748
Size: 5486 2753
BlpI GC'TnA_GC
Cuts at: 5656 5656
Size: 8239
Bme1580I G_kGCm'C
Cuts at: 1790 2349 2628 3343 3496 3603 3872 4366 4441
Size: 559 279 715 153 107 269 494 75
Cuts at: 4441 4485 6300 7456 1790
Size: 44 1815 1156 2573
Fragments arranged by size:
2573 1815 1156 715 559 494 279 269
153 107 75 44
BmgBI CAC'GTC
Cuts at: 1318 2529 4132 4329 4585 5326 1318
Size: 1211 1603 197 256 741 4231
Fragments arranged by size:
4231 1603 1211 741 256 197
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3433 3832 4525
Size: 416 53 83 399 24 2016 399 693
Cuts at: 4525 4753 5970 6928 442
Size: 228 1217 958 1753
Fragments arranged by size:
2016 1753 1217 958 693 416 399 399
228 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8239
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2998 4964 2998
Size: 1966 6273
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2120 2663 4392 4959 4994 5180 5549 6899
Size: 881 543 1729 567 35 186 369 1350
Cuts at: 6899 1239
Size: 2579
Fragments arranged by size:
2579 1729 1350 881 567 543 369 186
35
Bpu10I CC'TnA_GC
Cuts at: 3060 4803 3060
Size: 1743 6496
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6364 7229 7527 6364
Size: 865 298 7076
Fragments arranged by size:
7076 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6902 882
Size: 617 5403 2219
Fragments arranged by size:
5403 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3055 6030 493
Size: 711 1851 2975 2702
Fragments arranged by size:
2975 2702 1851 711
BsaBI GATnn'nnATC
Cuts at: 2150 5369 8233 2150
Size: 3219 2864 2156
Fragments arranged by size:
3219 2864 2156
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2095 3720 3843 4727
Size: 53 83 186 936 562 1625 123 884
Cuts at: 4727 5012 5345 6046 6428 7063 275
Size: 285 333 701 382 635 1451
Fragments arranged by size:
1625 1451 936 884 701 635 562 382
333 285 186 123 83 53
BsaWI w'CCGG_w
Cuts at: 2412 3195 6672 7070 7344 7491 7902 2412
Size: 783 3477 398 274 147 411 2749
Fragments arranged by size:
3477 2749 783 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2445 2475 2537 2567 3257
Size: 30 165 30 1042 30 62 30 690
Cuts at: 3257 3287 4086 4116 4365 4395 4481 4511 1178
Size: 30 799 30 249 30 86 30 4906
Fragments arranged by size:
4906 1042 799 690 249 165 86 62
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2651 2660 3029 3843 4723 5018 5156 1186
Size: 1465 9 369 814 880 295 138 4269
Fragments arranged by size:
4269 1465 880 814 369 295 138 9
BseYI C'CCAG_C
Cuts at: 2179 3569 4066 4142 4427 4705 7442 7938 2179
Size: 1390 497 76 285 278 2737 496 2480
Fragments arranged by size:
2737 2480 1390 497 496 285 278 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2155 2279 2303 2762 2765 3465 4243
Size: 573 419 124 24 459 3 700 778
Cuts at: 4243 4292 4537 4610 4790 5162 1163
Size: 49 245 73 180 372 4240
Fragments arranged by size:
4240 778 700 573 459 419 372 245
180 124 73 49 24 3
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2266 3181 3321 5689 6450 7478
Size: 275 186 265 915 140 2368 761 1028
Cuts at: 7478 1540
Size: 2301
Fragments arranged by size:
2368 2301 1028 915 761 275 265 186
140
BsiHKAI G_wGCw'C
Cuts at: 2349 2628 3003 3402 3894 3903 5028 5244 5424
Size: 279 375 399 492 9 1125 216 180
Cuts at: 5424 6300 6385 7456 2349
Size: 876 85 1071 3132
Fragments arranged by size:
3132 1125 1071 876 492 399 375 279
216 180 85 9
BsmI GAATG_Cn'
Cuts at: 5750 5843 5750
Size: 93 8146
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2349 2628 3003 3343 3402 3496 3603
Size: 399 559 279 375 340 59 94 107
Cuts at: 3603 3872 3894 3903 4366 4441 4485 4528 5028
Size: 269 22 9 463 75 44 43 500
Cuts at: 5028 5244 5424 5558 6300 6385 7456 1391
Size: 216 180 134 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 559 500 463 399 375
340 279 269 216 180 134 107 94
85 75 59 44 43 22 9
BspEI T'CCGG_A
Cuts at: 3195 7902 3195
Size: 4707 3532
BspHI T'CATG_A
Cuts at: 6128 7858 6128
Size: 1730 6509
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2301 2983 4632 4923 5218 5594 844
Size: 1457 682 1649 291 295 376 3489
Fragments arranged by size:
3489 1649 1457 682 376 295 291
BsrBI CCG'CTC
Cuts at: 1394 1823 2378 3201 4830 5164 6126 1394
Size: 429 555 823 1629 334 962 3507
Fragments arranged by size:
3507 1629 962 823 555 429 334
BsrDI GCAATG_nn'
Cuts at: 65 1248 2481 3291 6734 6908 65
Size: 1183 1233 810 3443 174 1396
Fragments arranged by size:
3443 1396 1233 1183 810 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2232 3176 4992 5216 6883 7070 1700
Size: 110 422 944 1816 224 1667 187 2869
Fragments arranged by size:
2869 1816 1667 944 422 224 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8239
BssHII G'CGCG_C
Cuts at: 1925 3155 1925
Size: 1230 7009
BssSI C'ACGA_G
Cuts at: 2546 3744 6299 7311 2546
Size: 1198 2555 1012 3474
Fragments arranged by size:
3474 2555 1198 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3397 4088 794
Size: 2603 691 4945
Fragments arranged by size:
4945 2603 691
BstBI TT'CG_AA
Cuts at: 7043 7043
Size: 8239
BstEII G'GTnAC_C
Cuts at: 1184 3136 5449 1184
Size: 1952 2313 3974
Fragments arranged by size:
3974 2313 1952
BstXI CCAn_nnnn'nTGG
Cuts at: 3949 4744 5437 3949
Size: 795 693 6751
Fragments arranged by size:
6751 795 693
BstYI r'GATC_y
Cuts at: 1972 2748 3048 4599 4960 5569 5936 6321 6338
Size: 776 300 1551 361 609 367 385 17
Cuts at: 6338 7790 7876 7888 8234 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1551 1452 776 609 385 367 361
346 300 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2036 3654 2036
Size: 1618 6621
BtgI C'CryG_G
Cuts at: 513 1065 2026 2266 2729 2931 3298 3315 3576
Size: 552 961 240 463 202 367 17 261
Cuts at: 3576 4251 4485 4833 4870 5460 513
Size: 675 234 348 37 590 3292
Fragments arranged by size:
3292 961 675 590 552 463 367 348
261 240 234 202 37 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2461 3364 487
Size: 683 56 1235 903 5362
Fragments arranged by size:
5362 1235 903 683 56
BtsI GCAGTG_nn'
Cuts at: 2349 3603 3731 4160 4304 5073 5755 6549 6569
Size: 1254 128 429 144 769 682 794 20
Cuts at: 6569 2349
Size: 4019
Fragments arranged by size:
4019 1254 794 769 682 429 144 128
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8239
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8204
DraI TTT'AAA
Cuts at: 5543 5543
Size: 8239
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3587 3977 7246 809
Size: 507 2271 390 3269 1802
Fragments arranged by size:
3269 2271 1802 507 390
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2130 2263 2319
Size: 54 1425 50 275 18 300 133 56
Cuts at: 2319 2679 3178 3318 3517 3738 3999 4134 4182
Size: 360 499 140 199 221 261 135 48
Cuts at: 4182 4233 4557 5193 5218 5397 5686 6575 8204
Size: 51 324 636 25 179 289 889 1629
Cuts at: 8204 8
Size: 43
Fragments arranged by size:
1629 1425 889 636 499 360 324 300
289 275 261 221 199 179 140 135
133 56 54 51 50 48 43 25
18
EagI C'GGCC_G
Cuts at: 1537 1812 2263 3178 3318 5686 1537
Size: 275 451 915 140 2368 4090
Fragments arranged by size:
4090 2368 915 451 275 140
EarI CTCTTCn'nnn_
Cuts at: 1353 3812 4182 4806 5120 6169 1353
Size: 2459 370 624 314 1049 3423
Fragments arranged by size:
3423 2459 1049 624 370 314
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2042 3209 3226 4823 6826 7200
Size: 1064 138 453 1167 17 1597 2003 374
Cuts at: 7200 7346 387
Size: 146 1280
Fragments arranged by size:
2003 1597 1280 1167 1064 453 374 146
138 17
EcoICRI GAG'CTC
Cuts at: 3892 3901 5242 3892
Size: 9 1341 6889
Fragments arranged by size:
6889 1341 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2030 2120 2663 3697 3781 3793 4265
Size: 393 398 90 543 1034 84 12 472
Cuts at: 4265 4392 4928 4959 4994 5180 5549 6302 6899
Size: 127 536 31 35 186 369 753 597
Cuts at: 6899 7686 7954 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1034 787 753 597 543 536 472
398 393 369 268 186 127 90 84
35 31 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2537 3092 2537
Size: 555 7684
EcoO109I rG'GnC_Cy
Cuts at: 2032 3148 3339 3340 3650 4437 4438 4750 4912
Size: 1116 191 1 310 787 1 312 162
Cuts at: 4912 5126 5314 2032
Size: 214 188 4957
Fragments arranged by size:
4957 1116 787 312 310 214 191 188
162 1 1
EcoRI G'AATT_C
Cuts at: 5548 5926 5548
Size: 378 7861
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2983 2983
Size: 8239
FseI GG_CCGG'CC
Cuts at: 5220 5220
Size: 8239
FspI TGC'GCA
Cuts at: 3752 6745 3752
Size: 2993 5246
FspAI rTGC'GCAy
Cuts at: 3752 3752
Size: 8239
HaeII r_GCGC'y
Cuts at: 1678 2098 2702 5348 7386 1678
Size: 420 604 2646 2038 2531
Fragments arranged by size:
2646 2531 2038 604 420
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3240 3272 4391 4423 5012
Size: 32 135 32 1668 32 1119 32 589
Cuts at: 5012 5044 5118 5150 6880 6912 6954 6986 1373
Size: 32 74 32 1730 32 42 32 2626
Fragments arranged by size:
2626 1730 1668 1119 589 135 74 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2980 5583 5829 669
Size: 988 281 1042 2603 246 3079
Fragments arranged by size:
3079 2603 1042 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8239
HpaI GTT'AAC
Cuts at: 5829 5829
Size: 8239
KasI G'GCGC_C
Cuts at: 2094 5344 2094
Size: 3250 4989
KpnI G_GTAC'C
Cuts at: 5564 5564
Size: 8239
MfeI C'AATT_G
Cuts at: 5838 5838
Size: 8239
MluI A'CGCG_T
Cuts at: 8115 8115
Size: 8239
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2580 3563 7353 7537 1305
Size: 1275 983 3790 184 2007
Fragments arranged by size:
3790 2007 1275 983 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2132 2321 3740 4001 5399
Size: 54 1425 343 300 189 1419 261 1398
Cuts at: 5399 10
Size: 2850
Fragments arranged by size:
2850 1425 1419 1398 343 300 261 189
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2156 2315 2618 2741 3188 3329 3746
Size: 648 990 159 303 123 447 141 417
Cuts at: 3746 4478 4721 5375 5435 6197 6556 6715 518
Size: 732 243 654 60 762 359 159 2042
Fragments arranged by size:
2042 990 762 732 654 648 447 417
359 303 243 159 159 141 123 60
MspA1I CmG'CkG
Cuts at: 1606 2075 2268 2809 3472 4397 5254 5655 6332
Size: 469 193 541 663 925 857 401 677
Cuts at: 6332 7480 7725 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 925 857 677 663 541 469
401 245 193
NaeI GCC'GGC
Cuts at: 5218 5218
Size: 8239
NarI GG'CG_CC
Cuts at: 2095 5345 2095
Size: 3250 4989
NcoI C'CATG_G
Cuts at: 513 1065 2026 4485 4870 5460 513
Size: 552 961 2459 385 590 3292
Fragments arranged by size:
3292 2459 961 590 552 385
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8239
NgoMIV G'CCGG_C
Cuts at: 5216 5216
Size: 8239
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8239
NotI GC'GGCC_GC
Cuts at: 5686 5686
Size: 8239
NspI r_CATG'y
Cuts at: 1132 1229 4063 5597 7142 1132
Size: 97 2834 1534 1545 2229
Fragments arranged by size:
2834 2229 1545 1534 97
PasI CC'CwG_GG
Cuts at: 1348 4604 1348
Size: 3256 4983
PciI A'CATG_T
Cuts at: 7138 7138
Size: 8239
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2824 3526 4645 4705 4765 1108
Size: 1716 702 1119 60 60 4582
Fragments arranged by size:
4582 1716 1119 702 60 60
PfoI T'CCnGG_A
Cuts at: 3894 4281 5526 3894
Size: 387 1245 6607
Fragments arranged by size:
6607 1245 387
PmeI GTTT'AAAC
Cuts at: 5543 5543
Size: 8239
PmlI CAC'GTG
Cuts at: 3055 3055
Size: 8239
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6308 6340 7821 7853 6308
Size: 32 1481 32 6694
Fragments arranged by size:
6694 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3148 4750 4912 5126 3148
Size: 1602 162 214 6261
Fragments arranged by size:
6261 1602 214 162
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8239
PsiI TTA'TAA
Cuts at: 5809 5809
Size: 8239
PspOMI G'GGCC_C
Cuts at: 3339 3492 4437 3339
Size: 153 945 7141
Fragments arranged by size:
7141 945 153
PspXI vC'TCGA_Gb
Cuts at: 3666 3666
Size: 8239
PstI C_TGCA'G
Cuts at: 830 2994 3393 3598 3888 4167 4851 4968 5124
Size: 2164 399 205 290 279 684 117 156
Cuts at: 5124 5591 830
Size: 467 3478
Fragments arranged by size:
3478 2164 684 467 399 290 279 205
156 117
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8239
PvuII CAG'CTG
Cuts at: 3472 4397 5254 3472
Size: 925 857 6457
Fragments arranged by size:
6457 925 857
SacI G_AGCT'C
Cuts at: 3894 3903 5244 3894
Size: 9 1341 6889
Fragments arranged by size:
6889 1341 9
SacII CC_GC'GG
Cuts at: 2269 2269
Size: 8239
SalI G'TCGA_C
Cuts at: 1936 5581 1936
Size: 3645 4594
SanDI GG'GwC_CC
Cuts at: 5126 5126
Size: 8239
SapI GCTCTTCn'nnn_
Cuts at: 3812 4182 4806 3812
Size: 370 624 7245
Fragments arranged by size:
7245 624 370
SbfI CC_TGCA'GG
Cuts at: 2994 4851 5124 5591 2994
Size: 1857 273 467 5642
Fragments arranged by size:
5642 1857 467 273
ScaI AGT'ACT
Cuts at: 4157 4169 4514 6487 8199 4157
Size: 12 345 1973 1712 4197
Fragments arranged by size:
4197 1973 1712 345 12
SexAI A'CCwGG_T
Cuts at: 3033 3033
Size: 8239
SfcI C'TryA_G
Cuts at: 826 1045 2990 3389 3594 3884 4163 4504 4847
Size: 219 1945 399 205 290 279 341 343
Cuts at: 4847 4964 5120 5587 6722 7403 7594 826
Size: 117 156 467 1135 681 191 1471
Fragments arranged by size:
1945 1471 1135 681 467 399 343 341
290 279 219 205 191 156 117
SfiI GGCCn_nnn'nGGCC
Cuts at: 4445 5396 4445
Size: 951 7288
SfoI GGC'GCC
Cuts at: 2096 5346 2096
Size: 3250 4989
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8239
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8239
SmaI CCC'GGG
Cuts at: 1872 2471 2660 3687 5566 1872
Size: 599 189 1027 1879 4545
Fragments arranged by size:
4545 1879 1027 599 189
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3666 6343 7244 7506 820
Size: 197 926 1723 2677 901 262 1553
Fragments arranged by size:
2677 1723 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6030 493
Size: 5537 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8239
SphI G_CATG'C
Cuts at: 1132 5597 1132
Size: 4465 3774
SrfI GCCC'GGGC
Cuts at: 2471 2471
Size: 8239
SspI AAT'ATT
Cuts at: 5 52 6163 5
Size: 47 6111 2081
Fragments arranged by size:
6111 2081 47
StuI AGG'CCT
Cuts at: 1517 2288 2393 4682 1517
Size: 771 105 2289 5074
Fragments arranged by size:
5074 2289 771 105
StyI C'CwwG_G
Cuts at: 513 1065 2026 2322 3244 4485 4870 4919 5310
Size: 552 961 296 922 1241 385 49 391
Cuts at: 5310 5391 5400 5460 5493 5678 513
Size: 81 9 60 33 185 3074
Fragments arranged by size:
3074 1241 961 922 552 391 385 296
185 81 60 49 33 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3452 3770 4223 4334 5294 5473 6266
Size: 1255 1872 318 453 111 960 179 793
Cuts at: 6266 6283 6436 6621 7066 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1872 1498 1255 960 793 453 445 318
185 179 153 111 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4155 4167 4512
Size: 274 80 33 51 1443 2178 12 345
Cuts at: 4512 4614 6485 8197 96
Size: 102 1871 1712 138
Fragments arranged by size:
2178 1871 1712 1443 345 274 138 102
80 51 33 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2445 2477 2537 2569 3257 3289 3535 3567 4086
Size: 32 60 32 688 32 246 32 519
Cuts at: 4086 4118 4296 4328 4365 4397 8199 8231 2445
Size: 32 178 32 37 32 3802 32 2453
Fragments arranged by size:
3802 2453 688 519 246 178 60 37
32 32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3035 1316
Size: 1719 6520
XbaI T'CTAG_A
Cuts at: 5575 5575
Size: 8239
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4063 4702 4063
Size: 639 7600
XhoI C'TCGA_G
Cuts at: 1943 3666 1943
Size: 1723 6516
XmaI C'CCGG_G
Cuts at: 1870 2469 2658 3685 5564 1870
Size: 599 189 1027 1879 4545
Fragments arranged by size:
4545 1879 1027 599 189
XmnI GAAnn'nnTTC
Cuts at: 4890 6368 4890
Size: 1478 6761
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3844 6047 276
Size: 53 83 186 936 2310 2203 2468
Fragments arranged by size:
2468 2310 2203 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I
DraIII EcoRV NruI NsiI PacI PsrI RsrII SwaI