(Circular) (Six-base) MAPSORT of: pFN21AB3647.seq Check: 6851 from: 1 to: 6499 pFN21AB3647 6499 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2559 2559 Size: 6499 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3435 4309 278 Size: 53 83 186 936 1899 874 2468 Fragments arranged by size: 2468 1899 936 874 186 83 53 AccI GT'mk_AC Cuts at: 1937 3051 3842 1937 Size: 1114 791 4594 Fragments arranged by size: 4594 1114 791 Acc65I G'GTAC_C Cuts at: 3348 3820 3348 Size: 472 6027 AclI AA'CG_TT Cuts at: 1563 4626 4999 1563 Size: 3063 373 3063 Fragments arranged by size: 3063 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2216 2343 2576 2621 4562 5946 6214 1632 Size: 584 127 233 45 1941 1384 268 1917 Fragments arranged by size: 1941 1917 1384 584 268 233 127 45 AfeI AGC'GCT Cuts at: 3656 3656 Size: 6499 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6302 AflIII A'CryG_T Cuts at: 1203 5398 6375 1203 Size: 4195 977 1327 Fragments arranged by size: 4195 1327 977 AgeI A'CCGG_T Cuts at: 5330 5330 Size: 6499 AhdI GACnn_n'nnGTC Cuts at: 5228 5228 Size: 6499 AleI CACnn'nnGTG Cuts at: 1166 3540 1166 Size: 2374 4125 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3558 1184 Size: 2374 4125 AlwNI CAG_nnn'CTG Cuts at: 3619 4242 5814 3619 Size: 623 1572 4304 Fragments arranged by size: 4304 1572 623 ApaLI G'TGCA_C Cuts at: 2519 4556 5712 2519 Size: 2037 1156 3306 Fragments arranged by size: 3306 2037 1156 ApoI r'AATT_y Cuts at: 1493 2385 2753 3337 3808 4034 4186 6295 1493 Size: 892 368 584 471 226 152 2109 1697 Fragments arranged by size: 2109 1697 892 584 471 368 226 152 AseI AT'TA_AT Cuts at: 160 1032 5053 160 Size: 872 4021 1606 Fragments arranged by size: 4021 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6499 AvaI C'yCGr_G Cuts at: 1870 1943 2639 3824 1870 Size: 73 696 1185 4545 Fragments arranged by size: 4545 1185 696 73 BamHI G'GATC_C Cuts at: 3034 3829 4196 3034 Size: 795 367 5337 Fragments arranged by size: 5337 795 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3348 3542 3820 5275 618 Size: 325 217 627 1561 194 278 1455 1842 Fragments arranged by size: 1842 1561 1455 627 325 278 217 194 BanII G_rGCy'C Cuts at: 1391 2265 2439 3818 1391 Size: 874 174 1379 4072 Fragments arranged by size: 4072 1379 874 174 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3020 928 Size: 971 1121 4407 Fragments arranged by size: 4407 1121 971 BbvCI CC'TCA_GC Cuts at: 2156 3210 2156 Size: 1054 5445 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4690 4724 1903 Size: 34 2753 34 3678 Fragments arranged by size: 3678 2753 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3489 4391 5601 3489 Size: 902 1210 4387 Fragments arranged by size: 4387 1210 902 BclI T'GATC_A Cuts at: 1548 1548 Size: 6499 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2147 3735 5110 Size: 107 122 71 631 605 475 1588 1375 Cuts at: 5110 136 Size: 1525 Fragments arranged by size: 1588 1525 1375 631 605 475 122 107 71 BglII A'GATC_T Cuts at: 6494 6494 Size: 6499 BlpI GC'TnA_GC Cuts at: 2772 3916 2772 Size: 1144 5355 Bme1580I G_kGCm'C Cuts at: 1790 2523 3547 3698 4560 5716 1790 Size: 733 1024 151 862 1156 2573 Fragments arranged by size: 2573 1156 1024 862 733 151 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6499 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3083 4230 5188 Size: 416 53 83 399 24 1666 1147 958 Cuts at: 5188 442 Size: 1753 Fragments arranged by size: 1753 1666 1147 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6499 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3213 3213 Size: 6499 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2234 3287 3476 3731 5159 1239 Size: 995 1053 189 255 1428 2579 Fragments arranged by size: 2579 1428 1053 995 255 189 Bpu10I CC'TnA_GC Cuts at: 2156 2337 3210 2156 Size: 181 873 5445 Fragments arranged by size: 5445 873 181 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2423 4624 5489 5787 2423 Size: 2201 865 298 3135 Fragments arranged by size: 3135 2201 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2940 5162 882 Size: 617 1441 2222 2219 Fragments arranged by size: 2222 2219 1441 617 BsaAI yAC'GTr Cuts at: 493 1204 2749 4290 493 Size: 711 1545 1541 2702 Fragments arranged by size: 2702 1545 1541 711 BsaBI GATnn'nnATC Cuts at: 2468 6493 2468 Size: 4025 2474 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2115 2940 3432 4306 Size: 53 83 186 936 582 825 492 874 Cuts at: 4306 4688 5323 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 874 825 635 582 492 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2407 2802 4932 5330 5604 5751 6162 2407 Size: 395 2130 398 274 147 411 2744 Fragments arranged by size: 2744 2130 411 398 395 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2426 2456 2888 2918 1178 Size: 30 165 30 1023 30 432 30 4759 Fragments arranged by size: 4759 1023 432 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2609 2630 3072 3554 1186 Size: 1423 21 442 482 4131 Fragments arranged by size: 4131 1423 482 442 21 BseYI C'CCAG_C Cuts at: 2766 2892 2971 3492 3612 5702 6198 2766 Size: 126 79 521 120 2090 496 3067 Fragments arranged by size: 3067 2090 521 496 126 120 79 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2879 3518 3745 1163 Size: 573 1143 639 227 3917 Fragments arranged by size: 3917 1143 639 573 227 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2135 3949 4710 5738 1540 Size: 275 186 134 1814 761 1028 2301 Fragments arranged by size: 2301 1814 1028 761 275 186 134 BsiHKAI G_wGCw'C Cuts at: 2073 2439 2523 4560 4645 5716 2073 Size: 366 84 2037 85 1071 2856 Fragments arranged by size: 2856 2037 1071 366 85 84 BsmI GAATG_Cn' Cuts at: 3199 4010 4103 3199 Size: 811 93 5595 Fragments arranged by size: 5595 811 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2106 2106 Size: 6499 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2265 2439 2523 3547 3698 3818 Size: 399 283 192 174 84 1024 151 120 Cuts at: 3818 4560 4645 5716 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 1024 742 399 283 192 174 151 120 85 84 BspEI T'CCGG_A Cuts at: 2407 6162 2407 Size: 3755 2744 BspHI T'CATG_A Cuts at: 3684 4388 6118 3684 Size: 704 1730 4065 Fragments arranged by size: 4065 1730 704 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2463 2559 2713 3267 3854 844 Size: 1619 96 154 554 587 3489 Fragments arranged by size: 3489 1619 587 554 154 96 BsrBI CCG'CTC Cuts at: 1394 1823 3145 4386 1394 Size: 429 1322 1241 3507 Fragments arranged by size: 3507 1322 1241 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4994 5168 65 Size: 1183 3746 174 1396 Fragments arranged by size: 3746 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2060 2088 2229 2776 5143 5330 1700 Size: 110 250 28 141 547 2367 187 2869 Fragments arranged by size: 2869 2367 547 250 187 141 110 28 BsrGI T'GTAC_A Cuts at: 96 2910 3243 3648 96 Size: 2814 333 405 2947 Fragments arranged by size: 2947 2814 405 333 BssHII G'CGCG_C Cuts at: 1925 2137 2160 1925 Size: 212 23 6264 Fragments arranged by size: 6264 212 23 BssSI C'ACGA_G Cuts at: 2123 4559 5571 2123 Size: 2436 1012 3051 Fragments arranged by size: 3051 2436 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6499 BstBI TT'CG_AA Cuts at: 5303 5303 Size: 6499 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6499 BstXI CCAn_nnnn'nTGG Cuts at: 2931 2931 Size: 6499 BstYI r'GATC_y Cuts at: 1972 2127 3034 3829 4196 4581 4598 6050 6136 Size: 155 907 795 367 385 17 1452 86 Cuts at: 6136 6148 6494 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 907 795 385 367 346 155 86 17 12 BstZ17I GTA'TAC Cuts at: 3052 3052 Size: 6499 BtgI C'CryG_G Cuts at: 513 1065 2004 2881 3487 513 Size: 552 939 877 606 3525 Fragments arranged by size: 3525 939 877 606 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2080 487 Size: 683 56 854 4906 Fragments arranged by size: 4906 854 683 56 BtsI GCAGTG_nn' Cuts at: 4015 4809 4829 4015 Size: 794 20 5685 Fragments arranged by size: 5685 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6499 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6464 DraI TTT'AAA Cuts at: 3327 3803 3327 Size: 476 6023 DraIII CAC_nnn'GTG Cuts at: 2427 2427 Size: 6499 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5506 809 Size: 507 4190 1802 Fragments arranged by size: 4190 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2079 2132 2148 Size: 54 1425 50 275 18 249 53 16 Cuts at: 2148 3946 4835 6464 8 Size: 1798 889 1629 43 Fragments arranged by size: 1798 1629 1425 889 275 249 54 53 50 43 18 16 EagI C'GGCC_G Cuts at: 1537 1812 2132 3946 1537 Size: 275 320 1814 4090 Fragments arranged by size: 4090 1814 320 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2014 2078 2586 2663 3204 3301 4429 1353 Size: 661 64 508 77 541 97 1128 3423 Fragments arranged by size: 3423 1128 661 541 508 97 77 64 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5086 5460 5606 387 Size: 1064 138 3497 374 146 1280 Fragments arranged by size: 3497 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2437 2437 Size: 6499 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2216 2234 2343 2576 2621 3287 3476 Size: 393 584 18 109 233 45 666 189 Cuts at: 3476 3731 4562 5159 5946 6214 1239 Size: 255 831 597 787 268 1524 Fragments arranged by size: 1524 831 787 666 597 584 393 268 255 233 189 109 45 18 EcoNI CCTnn'n_nnAGG Cuts at: 2555 2555 Size: 6499 EcoO109I rG'GnC_Cy Cuts at: 2296 2342 2452 2494 2605 2967 3112 3727 2296 Size: 46 110 42 111 362 145 615 5068 Fragments arranged by size: 5068 615 362 145 111 110 46 42 EcoRI G'AATT_C Cuts at: 2753 3808 4186 2753 Size: 1055 378 5066 Fragments arranged by size: 5066 1055 378 EcoRV GAT'ATC Cuts at: 2466 2466 Size: 6499 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2315 3544 2315 Size: 1229 5270 FspI TGC'GCA Cuts at: 2105 5005 2105 Size: 2900 3599 HaeII r_GCGC'y Cuts at: 1678 2172 2203 3658 5646 1678 Size: 494 31 1455 1988 2531 Fragments arranged by size: 2531 1988 1455 494 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2483 2515 2674 2706 3218 Size: 32 135 32 911 32 159 32 512 Cuts at: 3218 3250 5140 5172 5214 5246 1373 Size: 32 1890 32 42 32 2626 Fragments arranged by size: 2626 1890 911 512 159 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3001 3843 4089 669 Size: 988 281 1063 842 246 3079 Fragments arranged by size: 3079 1063 988 842 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6499 HpaI GTT'AAC Cuts at: 4089 4089 Size: 6499 KpnI G_GTAC'C Cuts at: 3352 3824 3352 Size: 472 6027 MfeI C'AATT_G Cuts at: 4098 4098 Size: 6499 MluI A'CGCG_T Cuts at: 6375 6375 Size: 6499 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5613 5797 1305 Size: 4308 184 2007 Fragments arranged by size: 4308 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2081 2150 10 Size: 54 1425 343 249 69 4359 Fragments arranged by size: 4359 1425 343 249 69 54 MslI CAynn'nnrTG Cuts at: 518 1166 2099 2483 2570 3540 4457 4816 4975 Size: 648 933 384 87 970 917 359 159 Cuts at: 4975 518 Size: 2042 Fragments arranged by size: 2042 970 933 917 648 384 359 159 87 MspA1I CmG'CkG Cuts at: 1606 2109 2236 2762 2863 2892 3265 3454 3579 Size: 503 127 526 101 29 373 189 125 Cuts at: 3579 3759 3915 4592 5740 5985 1606 Size: 180 156 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 526 503 373 245 189 180 156 127 125 101 29 NaeI GCC'GGC Cuts at: 2062 2090 2062 Size: 28 6471 NcoI C'CATG_G Cuts at: 513 1065 2004 3487 513 Size: 552 939 1483 3525 Fragments arranged by size: 3525 1483 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6499 NgoMIV G'CCGG_C Cuts at: 2060 2088 2060 Size: 28 6471 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6499 NotI GC'GGCC_GC Cuts at: 3946 3946 Size: 6499 NspI r_CATG'y Cuts at: 1132 1229 2911 3857 5402 1132 Size: 97 1682 946 1545 2229 Fragments arranged by size: 2229 1682 1545 946 97 PasI CC'CwG_GG Cuts at: 1348 3547 1348 Size: 2199 4300 PciI A'CATG_T Cuts at: 5398 5398 Size: 6499 PflMI CCAn_nnn'nTGG Cuts at: 1108 3619 1108 Size: 2511 3988 PfoI T'CCnGG_A Cuts at: 2181 2793 3274 3618 2181 Size: 612 481 344 5062 Fragments arranged by size: 5062 612 481 344 PmeI GTTT'AAAC Cuts at: 3803 3803 Size: 6499 PmlI CAC'GTG Cuts at: 2749 2749 Size: 6499 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4568 4600 6081 6113 4568 Size: 32 1481 32 4954 Fragments arranged by size: 4954 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2342 2452 2605 2967 3112 2342 Size: 110 153 362 145 5729 Fragments arranged by size: 5729 362 153 145 110 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6499 PsiI TTA'TAA Cuts at: 4069 4069 Size: 6499 PstI C_TGCA'G Cuts at: 830 3851 830 Size: 3021 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6499 PvuII CAG'CTG Cuts at: 2236 2892 3759 2236 Size: 656 867 4976 Fragments arranged by size: 4976 867 656 RsrII CG'GwC_CG Cuts at: 2514 2514 Size: 6499 SacI G_AGCT'C Cuts at: 2439 2439 Size: 6499 SalI G'TCGA_C Cuts at: 1936 3841 1936 Size: 1905 4594 SanDI GG'GwC_CC Cuts at: 2342 2342 Size: 6499 SapI GCTCTTCn'nnn_ Cuts at: 2078 3204 3301 2078 Size: 1126 97 5276 Fragments arranged by size: 5276 1126 97 SbfI CC_TGCA'GG Cuts at: 3851 3851 Size: 6499 ScaI AGT'ACT Cuts at: 2444 4747 6459 2444 Size: 2303 1712 2484 Fragments arranged by size: 2484 2303 1712 SfcI C'TryA_G Cuts at: 826 1045 3847 4982 5663 5854 826 Size: 219 2802 1135 681 191 1471 Fragments arranged by size: 2802 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6499 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6499 SmaI CCC'GGG Cuts at: 1872 2641 3826 1872 Size: 769 1185 4545 Fragments arranged by size: 4545 1185 769 SmlI C'TyrA_G Cuts at: 820 1017 1943 2438 4603 5504 5766 820 Size: 197 926 495 2165 901 262 1553 Fragments arranged by size: 2165 1553 926 901 495 262 197 SnaBI TAC'GTA Cuts at: 493 4290 493 Size: 3797 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6499 SphI G_CATG'C Cuts at: 1132 3857 1132 Size: 2725 3774 SspI AAT'ATT Cuts at: 5 52 3067 3474 4423 5 Size: 47 3015 407 949 2081 Fragments arranged by size: 3015 2081 949 407 47 StuI AGG'CCT Cuts at: 1517 2679 1517 Size: 1162 5337 StyI C'CwwG_G Cuts at: 513 1065 2004 2257 2448 3487 3519 3938 513 Size: 552 939 253 191 1039 32 419 3074 Fragments arranged by size: 3074 1039 939 552 419 253 191 32 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3530 4526 4543 4696 4881 5326 325 Size: 1255 1950 996 17 153 185 445 1498 Fragments arranged by size: 1950 1498 1255 996 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2442 2910 3243 Size: 274 80 33 51 1443 465 468 333 Cuts at: 3243 3648 4745 6457 96 Size: 405 1097 1712 138 Fragments arranged by size: 1712 1443 1097 468 465 405 333 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2888 2920 6459 6491 2888 Size: 32 3539 32 2896 Fragments arranged by size: 3539 2896 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3632 1316 Size: 2316 4183 XbaI T'CTAG_A Cuts at: 3835 3835 Size: 6499 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2354 2931 2354 Size: 577 5922 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6499 XmaI C'CCGG_G Cuts at: 1870 2639 3824 1870 Size: 769 1185 4545 Fragments arranged by size: 4545 1185 769 XmnI GAAnn'nnTTC Cuts at: 2565 3011 4628 2565 Size: 446 1617 4436 Fragments arranged by size: 4436 1617 446 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3433 4307 276 Size: 53 83 186 936 1899 874 2468 Fragments arranged by size: 2468 1899 936 874 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI Bsu36I FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI SacII SexAI SfiI SfoI SrfI SwaI