################################## # NO CUT ENZYMES ( 111 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspI AvrII BamHI BbeI BbrPI BciVI BclI BfaI BfrI BlnI BsaAI BseAI BsiWI BspDI BspEI BspHI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KasI KpnI KspI MaeI MfeI MluI MroI MseI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AhdI 1015 GACCTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1389 GTGCAC G^TGCAC ApaI 763 GGGCCC GGGCC^C ApaLI 1389 GTGCAC G^TGCAC AspEI 1015 GACCTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1412 TGCGCA TGC^GCA BbsI 498 GAAGAC GAAGAC(2/6) BcgI 1362 CGAGATGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 896 AGATCT A^GATCT BlpI 267 GCTGAGC GC^T[ACGT]AGC Bpu1102I 267 GCTGAGC GC^T[ACGT]AGC BpuAI 498 GAAGAC GAAGAC(2/6) BsaBI 18 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1531 GAGACC GGTCTC(1/5) BsiEI 1379 CGGCCG CG[GA][CT]^CG BsmBI 1022 CGTCTC CGTCTC(1/5) BsmI 1368 GAATGC GAATGC(1/-1) BspMI 972 GCAGGT ACCTGC(4/8) BsrDI 590 GCAATG GCAATG(2/0) BstAPI 528 GCAGTGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 267 GCTGAGC GC^T[ACGT]AGC EagI 1379 CGGCCG C^GGCCG Eam1105I 1015 GACCTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1263 CTCTTC CTCTTC(1/4) EclXI 1379 CGGCCG C^GGCCG Eco52I 1379 CGGCCG C^GGCCG EcoT14I 1430 CCAAGG C^C[AT][AT]GG FspI 1412 TGCGCA TGC^GCA Ksp632I 1263 CTCTTC CTCTTC(1/4) MamI 18 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC PflMI 724 CCAGCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 369 GAGCTC GAGCT^C SapI 1262 GCTCTTC GCTCTTC(1/4) ScaI 1322 AGTACT AGT^ACT SfiI 126 GGCCCACCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC StyI 1430 CCAAGG C^C[AT][AT]GG Van91I 724 CCAGCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcyI 1020 GGCGTC G[GA]^CG[CT]C 1459 GACGCC AvaI 375 CCCGAG C^[CT]CG[GA]G 982 CCCGAG BbvCI 243 CCTCAGC CCTCAGC(-5/-2) 1450 GCTGAGG BpmI 49 CTGGAG CTGGAG(16/14) 1105 CTGGAG BsaHI 1020 GGCGTC G[GA]^CG[CT]C 1459 GACGCC BsaWI 675 TCCGGT [AT]^CCGG[AT] 1223 ACCGGA BsgI 217 GTGCAG GTGCAG(16/14) 526 GTGCAG BsmAI 1531 GAGAC GTCTC(1/5) 1023 GTCTC BsoBI 375 CCCGAG C^[CT]CG[GA]G 982 CCCGAG BspLU11I 905 ACATGT A^CATGT 1385 ACATGT BsrBI 1260 CCGCTC CCGCTC(-3/-3) 778 GAGCGG BsrI 225 CCAGT ACTGG(1/-1) 288 CCAGT BssSI 1543 CACGAG CACGAG(-5/-1) 484 CTCGTG HaeII 190 AGCGCC [GA]GCGC^[CT] 802 AGCGCC Hin1I 1020 GGCGTC G[GA]^CG[CT]C 1459 GACGCC MaeIII 89 GTGAC ^GT[ACGT]AC 1114 GTGAC NaeI 207 GCCGGC GCC^GGC 263 GCCGGC NgoMIV 207 GCCGGC G^CCGGC 263 GCCGGC PvuII 1147 CAGCTG CAG^CTG 1242 CAGCTG SphI 399 GCATGC GCATG^C 533 GCATGC TaqI 1496 TCGA T^CGA 1529 TCGA Tsp45I 89 GTGAC ^GT[GC]AC 1114 GTGAC TthHB8I 1496 TCGA T^CGA 1529 TCGA XcmI 567 CCAGAAGGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 620 CCACGCCGGACCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwI AlwNI AspHI AvaII BalI BanI BanII BbvI BcnI BglI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI Bsp1286I Bsp1407I BsrFI BsrGI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI TseI TspRI XhoII