(Circular) (Six-base) MAPSORT of: pFN21AE1160.seq Check: 4051 from: 1 to: 6286 pFN21AE1160 6286 bp With 182 enzymes: SgfI * July 6, 2012 17:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4096 278 Size: 53 83 186 936 2560 2468 Fragments arranged by size: 2560 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3629 1937 Size: 1692 4594 Acc65I G'GTAC_C Cuts at: 3607 3607 Size: 6286 AclI AA'CG_TT Cuts at: 1563 4413 4786 1563 Size: 2850 373 3063 Fragments arranged by size: 3063 2850 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3594 4349 5733 6001 1632 Size: 1962 755 1384 268 1917 Fragments arranged by size: 1962 1917 1384 755 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6089 AflIII A'CryG_T Cuts at: 1203 2097 2910 3132 3390 5185 6162 1203 Size: 894 813 222 258 1795 977 1327 Fragments arranged by size: 1795 1327 977 894 813 258 222 AgeI A'CCGG_T Cuts at: 5117 5117 Size: 6286 AhdI GACnn_n'nnGTC Cuts at: 3025 5015 3025 Size: 1990 4296 AleI CACnn'nnGTG Cuts at: 1166 3131 1166 Size: 1965 4321 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2498 1184 Size: 1314 4972 AlwNI CAG_nnn'CTG Cuts at: 2017 2077 2086 2256 2299 2401 4029 5601 2017 Size: 60 9 170 43 102 1628 1572 2702 Fragments arranged by size: 2702 1628 1572 170 102 60 43 9 ApaI G_GGCC'C Cuts at: 2772 2772 Size: 6286 ApaLI G'TGCA_C Cuts at: 3394 4343 5499 3394 Size: 949 1156 4181 Fragments arranged by size: 4181 1156 949 ApoI r'AATT_y Cuts at: 1493 3595 3821 3973 6082 1493 Size: 2102 226 152 2109 1697 Fragments arranged by size: 2109 2102 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4840 160 Size: 872 3808 1606 Fragments arranged by size: 3808 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6286 AvaI C'yCGr_G Cuts at: 1870 1943 2380 2987 3611 1870 Size: 73 437 607 624 4545 Fragments arranged by size: 4545 624 607 437 73 BamHI G'GATC_C Cuts at: 3616 3983 3616 Size: 367 5919 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2351 2798 3125 3254 3607 Size: 325 217 627 564 447 327 129 353 Cuts at: 3607 5062 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 564 447 353 327 325 217 129 BanII G_rGCy'C Cuts at: 1391 2378 2433 2441 2772 3605 1391 Size: 987 55 8 331 833 4072 Fragments arranged by size: 4072 987 833 331 55 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2510 928 Size: 971 611 4704 Fragments arranged by size: 4704 971 611 BbvCI CC'TCA_GC Cuts at: 2249 3456 2249 Size: 1207 5079 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3356 3390 4477 4511 1903 Size: 34 1419 34 1087 34 3678 Fragments arranged by size: 3678 1419 1087 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4178 5388 4178 Size: 1210 5076 BclI T'GATC_A Cuts at: 1548 1548 Size: 6286 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2129 2138 3383 Size: 107 122 71 631 605 457 9 1245 Cuts at: 3383 3552 4897 136 Size: 169 1345 1525 Fragments arranged by size: 1525 1345 1245 631 605 457 169 122 107 71 9 BglII A'GATC_T Cuts at: 2901 6281 2901 Size: 3380 2906 BlpI GC'TnA_GC Cuts at: 2273 3703 2273 Size: 1430 4856 Bme1580I G_kGCm'C Cuts at: 1790 2354 2772 3398 4347 5503 1790 Size: 564 418 626 949 1156 2573 Fragments arranged by size: 2573 1156 949 626 564 418 BmgBI CAC'GTC Cuts at: 1318 2098 2778 3169 1318 Size: 780 680 391 4435 Fragments arranged by size: 4435 780 680 391 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2224 4017 4975 Size: 416 53 83 399 24 807 1793 958 Cuts at: 4975 442 Size: 1753 Fragments arranged by size: 1793 1753 958 807 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6286 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2542 2542 Size: 6286 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2075 3131 4946 1239 Size: 836 1056 1815 2579 Fragments arranged by size: 2579 1815 1056 836 Bpu10I CC'TnA_GC Cuts at: 2249 3456 2249 Size: 1207 5079 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2351 2788 3434 4411 5276 5574 2351 Size: 437 646 977 865 298 3063 Fragments arranged by size: 3063 977 865 646 437 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3530 4949 882 Size: 617 2031 1419 2219 Fragments arranged by size: 2219 2031 1419 617 BsaAI yAC'GTr Cuts at: 493 1204 4077 493 Size: 711 2873 2702 Fragments arranged by size: 2873 2702 711 BsaBI GATnn'nnATC Cuts at: 2027 6280 2027 Size: 4253 2033 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3026 3465 4093 4475 Size: 53 83 186 936 1493 439 628 382 Cuts at: 4475 5110 275 Size: 635 1451 Fragments arranged by size: 1493 1451 936 635 628 439 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2680 3228 4719 5117 5391 5538 5949 2680 Size: 548 1491 398 274 147 411 3017 Fragments arranged by size: 3017 1491 548 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6061 Fragments arranged by size: 6061 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2426 2861 2870 2873 2888 1186 Size: 1240 435 9 3 15 4584 Fragments arranged by size: 4584 1240 435 15 9 3 BseYI C'CCAG_C Cuts at: 2582 2697 2771 5489 5985 2582 Size: 115 74 2718 496 2883 Fragments arranged by size: 2883 2718 496 115 74 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2243 2552 1163 Size: 573 507 309 4897 Fragments arranged by size: 4897 573 507 309 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3387 3736 4497 5525 1540 Size: 275 186 1386 349 761 1028 2301 Fragments arranged by size: 2301 1386 1028 761 349 275 186 BsiHKAI G_wGCw'C Cuts at: 2378 3315 3398 3450 4347 4432 5503 2378 Size: 937 83 52 897 85 1071 3161 Fragments arranged by size: 3161 1071 937 897 85 83 52 BsmI GAATG_Cn' Cuts at: 3379 3797 3890 3379 Size: 418 93 5775 Fragments arranged by size: 5775 418 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3033 3033 Size: 6286 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2354 2378 2433 2441 2772 3315 3398 Size: 399 564 24 55 8 331 543 83 Cuts at: 3398 3450 3605 4347 4432 5503 1391 Size: 52 155 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 564 543 399 331 155 85 83 55 52 24 8 BspEI T'CCGG_A Cuts at: 5949 5949 Size: 6286 BspHI T'CATG_A Cuts at: 4175 5905 4175 Size: 1730 4556 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2968 3574 3641 844 Size: 2124 606 67 3489 Fragments arranged by size: 3489 2124 606 67 BsrBI CCG'CTC Cuts at: 1394 1823 2785 3267 4173 1394 Size: 429 962 482 906 3507 Fragments arranged by size: 3507 962 906 482 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2602 4781 4955 65 Size: 1183 1354 2179 174 1396 Fragments arranged by size: 2179 1396 1354 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2212 2268 2646 2732 2895 3257 4930 Size: 110 402 56 378 86 163 362 1673 Cuts at: 4930 5117 1700 Size: 187 2869 Fragments arranged by size: 2869 1673 402 378 362 187 163 110 86 56 BsrGI T'GTAC_A Cuts at: 96 2307 2913 2922 3135 96 Size: 2211 606 9 213 3247 Fragments arranged by size: 3247 2211 606 213 9 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6286 BssSI C'ACGA_G Cuts at: 2489 3548 4346 5358 2489 Size: 1059 798 1012 3417 Fragments arranged by size: 3417 1059 1012 798 BstAPI GCAn_nnn'nTGC Cuts at: 794 2539 794 Size: 1745 4541 BstBI TT'CG_AA Cuts at: 5090 5090 Size: 6286 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6286 BstYI r'GATC_y Cuts at: 1972 2028 2244 2328 2901 3616 3983 4368 4385 Size: 56 216 84 573 715 367 385 17 Cuts at: 4385 5837 5923 5935 6281 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 715 573 385 367 346 216 86 84 56 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2199 2442 2544 2706 513 Size: 552 939 195 243 102 162 4093 Fragments arranged by size: 4093 939 552 243 195 162 102 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3364 487 Size: 683 56 2138 3409 Fragments arranged by size: 3409 2138 683 56 BtsI GCAGTG_nn' Cuts at: 2015 2540 3802 4596 4616 2015 Size: 525 1262 794 20 3685 Fragments arranged by size: 3685 1262 794 525 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6286 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6251 DraI TTT'AAA Cuts at: 3590 3590 Size: 6286 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5293 809 Size: 507 3977 1802 Fragments arranged by size: 3977 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2462 2547 2569 Size: 54 1425 50 275 18 632 85 22 Cuts at: 2569 2613 2941 3240 3384 3733 4622 6251 8 Size: 44 328 299 144 349 889 1629 43 Fragments arranged by size: 1629 1425 889 632 349 328 299 275 144 85 54 50 44 43 22 18 EagI C'GGCC_G Cuts at: 1537 1812 3384 3733 1537 Size: 275 1572 349 4090 Fragments arranged by size: 4090 1572 349 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3274 4216 1353 Size: 1921 942 3423 Fragments arranged by size: 3423 1921 942 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4873 5247 5393 387 Size: 1064 138 3284 374 146 1280 Fragments arranged by size: 3284 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2376 2376 Size: 6286 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 3131 3594 4349 4946 5733 6001 Size: 393 443 1056 463 755 597 787 268 Cuts at: 6001 1239 Size: 1524 Fragments arranged by size: 1524 1056 787 755 597 463 443 393 268 EcoO109I rG'GnC_Cy Cuts at: 2083 2173 2398 2416 2965 3193 3563 2083 Size: 90 225 18 549 228 370 4806 Fragments arranged by size: 4806 549 370 228 225 90 18 EcoRI G'AATT_C Cuts at: 3595 3973 3595 Size: 378 5908 FspI TGC'GCA Cuts at: 3419 4792 3419 Size: 1373 4913 HaeII r_GCGC'y Cuts at: 1678 2199 2811 5433 1678 Size: 521 612 2622 2531 Fragments arranged by size: 2622 2531 612 521 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2405 2437 3011 3043 4927 Size: 32 135 32 833 32 574 32 1884 Cuts at: 4927 4959 5001 5033 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1884 833 574 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3630 3876 669 Size: 988 281 1692 246 3079 Fragments arranged by size: 3079 1692 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6286 HpaI GTT'AAC Cuts at: 3876 3876 Size: 6286 KpnI G_GTAC'C Cuts at: 3611 3611 Size: 6286 MfeI C'AATT_G Cuts at: 3885 3885 Size: 6286 MluI A'CGCG_T Cuts at: 6162 6162 Size: 6286 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2406 2502 2760 3269 5400 5584 1305 Size: 1101 96 258 509 2131 184 2007 Fragments arranged by size: 2131 2007 1101 509 258 184 96 MscI TGG'CCA Cuts at: 10 64 1489 1832 2464 2549 2571 2615 3242 Size: 54 1425 343 632 85 22 44 627 Cuts at: 3242 10 Size: 3054 Fragments arranged by size: 3054 1425 632 627 343 85 54 44 22 MslI CAynn'nnrTG Cuts at: 518 1166 2282 2543 3131 4244 4603 4762 518 Size: 648 1116 261 588 1113 359 159 2042 Fragments arranged by size: 2042 1116 1113 648 588 359 261 159 MspA1I CmG'CkG Cuts at: 1606 2074 2482 3066 3154 3249 3702 4379 5527 Size: 468 408 584 88 95 453 677 1148 Cuts at: 5527 5772 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 584 468 453 408 245 95 88 NaeI GCC'GGC Cuts at: 2214 2270 2214 Size: 56 6230 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4795 Fragments arranged by size: 4795 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6286 NgoMIV G'CCGG_C Cuts at: 2212 2268 2212 Size: 56 6230 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6286 NotI GC'GGCC_GC Cuts at: 3733 3733 Size: 6286 NspI r_CATG'y Cuts at: 1132 1229 2408 2542 2914 3394 3644 5189 1132 Size: 97 1179 134 372 480 250 1545 2229 Fragments arranged by size: 2229 1545 1179 480 372 250 134 97 PasI CC'CwG_GG Cuts at: 1348 2395 1348 Size: 1047 5239 PciI A'CATG_T Cuts at: 2910 3390 5185 2910 Size: 480 1795 4011 Fragments arranged by size: 4011 1795 480 PflMI CCAn_nnn'nTGG Cuts at: 1108 2735 1108 Size: 1627 4659 PfoI T'CCnGG_A Cuts at: 2052 2169 2331 2970 2052 Size: 117 162 639 5368 Fragments arranged by size: 5368 639 162 117 PmeI GTTT'AAAC Cuts at: 3590 3590 Size: 6286 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4355 4387 5868 5900 4355 Size: 32 1481 32 4741 Fragments arranged by size: 4741 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2173 3193 3563 2173 Size: 1020 370 4896 Fragments arranged by size: 4896 1020 370 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6286 PsiI TTA'TAA Cuts at: 3856 3856 Size: 6286 PspOMI G'GGCC_C Cuts at: 2768 2768 Size: 6286 PstI C_TGCA'G Cuts at: 830 2022 2082 2328 3585 3638 830 Size: 1192 60 246 1257 53 3478 Fragments arranged by size: 3478 1257 1192 246 60 53 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6286 PvuII CAG'CTG Cuts at: 3154 3249 3154 Size: 95 6191 SacI G_AGCT'C Cuts at: 2378 2378 Size: 6286 SalI G'TCGA_C Cuts at: 1936 3628 1936 Size: 1692 4594 SanDI GG'GwC_CC Cuts at: 3563 3563 Size: 6286 SapI GCTCTTCn'nnn_ Cuts at: 3274 3274 Size: 6286 SbfI CC_TGCA'GG Cuts at: 3585 3638 3585 Size: 53 6233 ScaI AGT'ACT Cuts at: 3329 4534 6246 3329 Size: 1205 1712 3369 Fragments arranged by size: 3369 1712 1205 SfcI C'TryA_G Cuts at: 826 1045 2018 2078 2324 2470 3581 3634 4769 Size: 219 973 60 246 146 1111 53 1135 Cuts at: 4769 5450 5641 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1111 973 681 246 219 191 146 60 53 SfiI GGCCn_nnn'nGGCC Cuts at: 2138 2138 Size: 6286 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6286 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6286 SmaI CCC'GGG Cuts at: 1872 3613 1872 Size: 1741 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2366 2803 3449 4390 5291 5553 Size: 197 926 423 437 646 941 901 262 Cuts at: 5553 820 Size: 1553 Fragments arranged by size: 1553 941 926 901 646 437 423 262 197 SnaBI TAC'GTA Cuts at: 493 4077 493 Size: 3584 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6286 SphI G_CATG'C Cuts at: 1132 2408 2542 3644 1132 Size: 1276 134 1102 3774 Fragments arranged by size: 3774 1276 1102 134 SspI AAT'ATT Cuts at: 5 52 4210 5 Size: 47 4158 2081 Fragments arranged by size: 4158 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6286 StyI C'CwwG_G Cuts at: 513 1065 2004 3435 3725 513 Size: 552 939 1431 290 3074 Fragments arranged by size: 3074 1431 939 552 290 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3506 4313 4330 4483 4668 5113 325 Size: 1255 1926 807 17 153 185 445 1498 Fragments arranged by size: 1926 1498 1255 807 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2088 2307 2913 Size: 274 80 33 51 1443 111 219 606 Cuts at: 2913 2922 3135 3327 4532 6244 96 Size: 9 213 192 1205 1712 138 Fragments arranged by size: 1712 1443 1205 606 274 219 213 192 138 111 80 51 33 9 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6246 6278 6246 Size: 32 6254 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6286 XbaI T'CTAG_A Cuts at: 3622 3622 Size: 6286 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2579 2632 2579 Size: 53 6233 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6286 XmaI C'CCGG_G Cuts at: 1870 3611 1870 Size: 1741 4545 XmnI GAAnn'nnTTC Cuts at: 4415 4415 Size: 6286 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4094 276 Size: 53 83 186 936 2560 2468 Fragments arranged by size: 2560 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SexAI SfoI SrfI SwaI