(Linear) (Six-base) MAPSORT of: KIEE1160.seq Check: 5016 from: 1 to: 1581 KIEE1160.orf With 182 enzymes: SgfI * July 3, 2012 16:48 .. AflIII A'CryG_T Cuts at: 0 92 905 1127 1385 1581 Size: 92 813 222 258 196 Fragments arranged by size: 813 258 222 196 92 AhdI GACnn_n'nnGTC Cuts at: 0 1020 1581 Size: 1020 561 AleI CACnn'nnGTG Cuts at: 0 1126 1581 Size: 1126 455 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 493 1581 Size: 493 1088 AlwNI CAG_nnn'CTG Cuts at: 0 12 72 81 251 294 396 1581 Size: 12 60 9 170 43 102 1185 Fragments arranged by size: 1185 170 102 60 43 12 9 ApaI G_GGCC'C Cuts at: 0 767 1581 Size: 767 814 ApaLI G'TGCA_C Cuts at: 0 1389 1581 Size: 1389 192 AvaI C'yCGr_G Cuts at: 0 375 982 1581 Size: 375 607 599 Fragments arranged by size: 607 599 375 BanI G'GyrC_C Cuts at: 0 346 793 1120 1249 1581 Size: 346 447 327 129 332 Fragments arranged by size: 447 346 332 327 129 BanII G_rGCy'C Cuts at: 0 373 428 436 767 1581 Size: 373 55 8 331 814 Fragments arranged by size: 814 373 331 55 8 BbsI GAAGACnn'nnnn_ Cuts at: 0 505 1581 Size: 505 1076 BbvCI CC'TCA_GC Cuts at: 0 244 1451 1581 Size: 244 1207 130 Fragments arranged by size: 1207 244 130 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1351 1385 1581 Size: 1351 34 196 Fragments arranged by size: 1351 196 34 BglI GCCn_nnn'nGGC Cuts at: 0 124 133 1378 1547 1581 Size: 124 9 1245 169 34 Fragments arranged by size: 1245 169 124 34 9 BglII A'GATC_T Cuts at: 0 896 1581 Size: 896 685 BlpI GC'TnA_GC Cuts at: 0 268 1581 Size: 268 1313 Bme1580I G_kGCm'C Cuts at: 0 349 767 1393 1581 Size: 349 418 626 188 Fragments arranged by size: 626 418 349 188 BmgBI CAC'GTC Cuts at: 0 93 773 1164 1581 Size: 93 680 391 417 Fragments arranged by size: 680 417 391 93 BmrI ACTGGGnnnn_n' Cuts at: 0 219 1581 Size: 219 1362 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 537 1581 Size: 537 1044 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 70 1126 1581 Size: 70 1056 455 Fragments arranged by size: 1056 455 70 Bpu10I CC'TnA_GC Cuts at: 0 244 1451 1581 Size: 244 1207 130 Fragments arranged by size: 1207 244 130 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 346 783 1429 1581 Size: 346 437 646 152 Fragments arranged by size: 646 437 346 152 BsaI GGTCTCn'nnnn_ Cuts at: 0 1525 1581 Size: 1525 56 BsaBI GATnn'nnATC Cuts at: 0 22 1581 Size: 22 1559 BsaHI Gr'CG_yC Cuts at: 0 1021 1460 1581 Size: 1021 439 121 Fragments arranged by size: 1021 439 121 BsaWI w'CCGG_w Cuts at: 0 675 1223 1581 Size: 675 548 358 Fragments arranged by size: 675 548 358 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 421 856 865 868 883 1581 Size: 421 435 9 3 15 698 Fragments arranged by size: 698 435 421 15 9 3 BseYI C'CCAG_C Cuts at: 0 577 692 766 1581 Size: 577 115 74 815 Fragments arranged by size: 815 577 115 74 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 238 547 1581 Size: 238 309 1034 Fragments arranged by size: 1034 309 238 BsiEI CG_ry'CG Cuts at: 0 1382 1581 Size: 1382 199 BsiHKAI G_wGCw'C Cuts at: 0 373 1310 1393 1445 1581 Size: 373 937 83 52 136 Fragments arranged by size: 937 373 136 83 52 BsmI GAATG_Cn' Cuts at: 0 1374 1581 Size: 1374 207 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1028 1581 Size: 1028 553 Bsp1286I G_dGCh'C Cuts at: 0 349 373 428 436 767 1310 1393 1445 Size: 349 24 55 8 331 543 83 52 Cuts at: 1445 1581 Size: 136 Fragments arranged by size: 543 349 331 136 83 55 52 24 8 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 963 1581 Size: 963 618 BsrBI CCG'CTC Cuts at: 0 780 1262 1581 Size: 780 482 319 Fragments arranged by size: 780 482 319 BsrDI GCAATG_nn' Cuts at: 0 597 1581 Size: 597 984 BsrFI r'CCGG_y Cuts at: 0 207 263 641 727 890 1252 1581 Size: 207 56 378 86 163 362 329 Fragments arranged by size: 378 362 329 207 163 86 56 BsrGI T'GTAC_A Cuts at: 0 302 908 917 1130 1581 Size: 302 606 9 213 451 Fragments arranged by size: 606 451 302 213 9 BssSI C'ACGA_G Cuts at: 0 484 1543 1581 Size: 484 1059 38 Fragments arranged by size: 1059 484 38 BstAPI GCAn_nnn'nTGC Cuts at: 0 534 1581 Size: 534 1047 BstYI r'GATC_y Cuts at: 0 23 239 323 896 1581 Size: 23 216 84 573 685 Fragments arranged by size: 685 573 216 84 23 BtgI C'CryG_G Cuts at: 0 194 437 539 701 1581 Size: 194 243 102 162 880 Fragments arranged by size: 880 243 194 162 102 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1359 1581 Size: 1359 222 BtsI GCAGTG_nn' Cuts at: 0 10 535 1581 Size: 10 525 1046 Fragments arranged by size: 1046 525 10 EaeI y'GGCC_r Cuts at: 0 457 542 564 608 936 1235 1379 1581 Size: 457 85 22 44 328 299 144 202 Fragments arranged by size: 457 328 299 202 144 85 44 22 EagI C'GGCC_G Cuts at: 0 1379 1581 Size: 1379 202 EarI CTCTTCn'nnn_ Cuts at: 0 1269 1581 Size: 1269 312 EcoICRI GAG'CTC Cuts at: 0 371 1581 Size: 371 1210 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 70 1126 1581 Size: 70 1056 455 Fragments arranged by size: 1056 455 70 EcoO109I rG'GnC_Cy Cuts at: 0 78 168 393 411 960 1188 1558 1581 Size: 78 90 225 18 549 228 370 23 Fragments arranged by size: 549 370 228 225 90 78 23 18 FspI TGC'GCA Cuts at: 0 1414 1581 Size: 1414 167 HaeII r_GCGC'y Cuts at: 0 194 806 1581 Size: 194 612 775 Fragments arranged by size: 775 612 194 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 400 432 1006 1038 1581 Size: 400 32 574 32 543 Fragments arranged by size: 574 543 400 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 401 497 755 1264 1581 Size: 401 96 258 509 317 Fragments arranged by size: 509 401 317 258 96 MscI TGG'CCA Cuts at: 0 459 544 566 610 1237 1581 Size: 459 85 22 44 627 344 Fragments arranged by size: 627 459 344 85 44 22 MslI CAynn'nnrTG Cuts at: 0 277 538 1126 1581 Size: 277 261 588 455 Fragments arranged by size: 588 455 277 261 MspA1I CmG'CkG Cuts at: 0 69 477 1061 1149 1244 1581 Size: 69 408 584 88 95 337 Fragments arranged by size: 584 408 337 95 88 69 NaeI GCC'GGC Cuts at: 0 209 265 1581 Size: 209 56 1316 Fragments arranged by size: 1316 209 56 NgoMIV G'CCGG_C Cuts at: 0 207 263 1581 Size: 207 56 1318 Fragments arranged by size: 1318 207 56 NspI r_CATG'y Cuts at: 0 403 537 909 1389 1581 Size: 403 134 372 480 192 Fragments arranged by size: 480 403 372 192 134 PasI CC'CwG_GG Cuts at: 0 390 1581 Size: 390 1191 PciI A'CATG_T Cuts at: 0 905 1385 1581 Size: 905 480 196 Fragments arranged by size: 905 480 196 PflMI CCAn_nnn'nTGG Cuts at: 0 730 1581 Size: 730 851 PfoI T'CCnGG_A Cuts at: 0 47 164 326 965 1581 Size: 47 117 162 639 616 Fragments arranged by size: 639 616 162 117 47 PpuMI rG'GwC_Cy Cuts at: 0 168 1188 1558 1581 Size: 168 1020 370 23 Fragments arranged by size: 1020 370 168 23 PspOMI G'GGCC_C Cuts at: 0 763 1581 Size: 763 818 PstI C_TGCA'G Cuts at: 0 17 77 323 1580 1581 Size: 17 60 246 1257 1 Fragments arranged by size: 1257 246 60 17 1 PvuII CAG'CTG Cuts at: 0 1149 1244 1581 Size: 1149 95 337 Fragments arranged by size: 1149 337 95 SacI G_AGCT'C Cuts at: 0 373 1581 Size: 373 1208 SanDI GG'GwC_CC Cuts at: 0 1558 1581 Size: 1558 23 SapI GCTCTTCn'nnn_ Cuts at: 0 1269 1581 Size: 1269 312 ScaI AGT'ACT Cuts at: 0 1324 1581 Size: 1324 257 SfcI C'TryA_G Cuts at: 0 13 73 319 465 1576 1581 Size: 13 60 246 146 1111 5 Fragments arranged by size: 1111 246 146 60 13 5 SfiI GGCCn_nnn'nGGCC Cuts at: 0 133 1581 Size: 133 1448 SmlI C'TyrA_G Cuts at: 0 361 798 1444 1581 Size: 361 437 646 137 Fragments arranged by size: 646 437 361 137 SphI G_CATG'C Cuts at: 0 403 537 1581 Size: 403 134 1044 Fragments arranged by size: 1044 403 134 StyI C'CwwG_G Cuts at: 0 1430 1581 Size: 1430 151 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1501 1581 Size: 1501 80 TatI w'GTAC_w Cuts at: 0 83 302 908 917 1130 1322 1581 Size: 83 219 606 9 213 192 259 Fragments arranged by size: 606 259 219 213 192 83 9 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 574 627 1581 Size: 574 53 954 Fragments arranged by size: 954 574 53 Enzymes that do cut: AflIII AhdI AleI AlfI AlwNI ApaI ApaLI AvaI BanI BanII BbsI BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstYI BtgI BtgZI BtsI EaeI EagI EarI EcoICRI Eco57MI EcoO109I FspI HaeII Hin4I MmeI MscI MslI MspA1I NaeI NgoMIV NspI PasI PciI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SanDI SapI ScaI SfcI SfiI SmlI SphI StyI TaqII TatI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AgeI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI BclI BfrBI BmtI BsaAI BsaXI BsiWI BspEI BspHI BssHII BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EciI EcoNI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NarI NcoI NdeI NheI NotI NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SbfI SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI XmaI XmnI ZraI