Restriction map for
pFN21AB3314
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AccI AccIII AclI AgeI AocI
Aor51HI ApaI AscI AvaI AviII
AvrII BamHI BbeI BglI BlnI
BlpI Bpu1102I BsaI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII Bst1107I BstAPI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I CpoI DrdI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HpaI KasI Ksp632I MfeI
MluI MroI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PaeR7I
PflMI PinAI PmeI PshAI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 61 enzymes) #
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Acc65I 1572 GGTACC G^GTACC
AflII 181 CTTAAG C^TTAAG
Alw44I 1540 GTGCAC G^TGCAC
AlwNI 284 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1540 GTGCAC G^TGCAC
AseI 1356 ATTAAT AT^TAAT
AsnI 1356 ATTAAT AT^TAAT
Asp700I 1868 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1572 GGTACC G^GTACC
BalI 1013 TGGCCA TGG^CCA
BanI 1572 GGTACC G^G[CT][GA]CC
BanII 265 GAGCTC G[GA]GC[CT]^C
BbrPI 1938 CACGTG CAC^GTG
BbsI 1056 GAAGAC GAAGAC(2/6)
BbvCI 99 GCTGAGG CCTCAGC(-5/-2)
BcgI 1542 GCACATCAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 593 TGATCA T^GATCA
BcnI 374 CCCGG CC^[GC]GG
BfaI 2006 CTAG C^TAG
BfrI 181 CTTAAG C^TTAAG
BpuAI 1056 GAAGAC GAAGAC(2/6)
BsaAI 1938 CACGTG [CT]AC^GT[GA]
BsaBI 2041 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI 1360 ATCGAT AT^CGAT
BspMI 53 ACCTGC ACCTGC(4/8)
BsrDI 1051 GCAATG GCAATG(2/0)
BssSI 1760 CTCGTG CACGAG(-5/-1)
BstBI 986 TTCGAA TT^CGAA
CfoI 1676 GCGC GCG^C
ClaI 1360 ATCGAT AT^CGAT
DraIII 394 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 93 CCGCGG C^C[GA][CT]GG
FauI 95 GCGGG CCCGC(4/6)
FbaI 593 TGATCA T^GATCA
HgaI 1977 GCGTC GACGC(5/10)
HhaI 1676 GCGC GCG^C
HinP1I 1676 GCGC G^CGC
HincII 1501 GTTGAC GT[CT]^[GA]AC
HindII 1501 GTTGAC GT[CT]^[GA]AC
HindIII 164 AAGCTT A^AGCTT
KpnI 1572 GGTACC GGTAC^C
KspI 93 CCGCGG CCGC^GG
MaeI 2006 CTAG C^TAG
MamI 2041 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1013 TGGCCA TGG^CCA
MscI 1013 TGGCCA TGG^CCA
NciI 374 CCCGG CC^[GC]GG
NspV 986 TTCGAA TT^CGAA
PmaCI 1938 CACGTG CAC^GTG
PmlI 1938 CACGTG CAC^GTG
PpuMI 1151 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 1356 ATTAAT AT^TAAT
PstI 1066 CTGCAG CTGCA^G
SacI 265 GAGCTC GAGCT^C
SacII 93 CCGCGG CCGC^GG
SfcI 1066 CTGCAG C^T[GA][CT]AG
SfuI 986 TTCGAA TT^CGAA
SmlI 181 CTTAAG C^T[CT][GA]AG
SspI 946 AATATT AAT^ATT
XcmI 1138 CCAGCAAAAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1868 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AatII 514 GACGTC GACGT^C
1701 GACGTC
AccII 94 CGCG CG^CG
332 CGCG
AcyI 514 GACGTC G[GA]^CG[CT]C
1701 GACGTC
AflIII 580 ACACGT A^C[GA][CT]GT
1939 ACGTGT
AspHI 265 GAGCTC G[AT]GC[AT]^C
1540 GTGCAC
AspI 846 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1170 GACAATGTC
BciVI 252 GGATAC GTATCC(6/5)
655 GGATAC
BsaHI 514 GACGTC G[GA]^CG[CT]C
1701 GACGTC
BsaWI 60 ACCGGA [AT]^CCGG[AT]
817 ACCGGA
BsiHKAI 265 GAGCTC G[AT]GC[AT]^C
1540 GTGCAC
BstUI 94 CGCG CG^CG
332 CGCG
DraI 1516 TTTAAA TTT^AAA
1984 TTTAAA
DraII 641 AGGCCCT [GA]G^G[ACGT]CC[CT]
1151 GGGACCT
EaeI 1013 TGGCCA [CT]^GGCC[GA]
1622 TGGCCG
EcoNI 525 CCTCAATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
960 CCTCTCAAAGG
EcoO109I 641 AGGCCCT [GA]G^G[ACGT]CC[CT]
1151 GGGACCT
Hin1I 514 GACGTC G[GA]^CG[CT]C
1701 GACGTC
MslI 901 CATTTAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1017 CACAGATATG
MvnI 94 CGCG CG^CG
332 CGCG
PflFI 846 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1170 GACAATGTC
PvuII 470 CAGCTG CAG^CTG
791 CAGCTG
Tth111I 846 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1170 GACAATGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AfaI AhdI AluI
AlwI ApoI AspEI AvaII BbvI
BglII BmyI BpmI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
Eam1105I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII