Restriction map for
pFN21AB3314
(Linear) (Six-base) MAPSORT of: KIBB3314.seq Check: 9366 from: 1 to: 2127
KIBB3314.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AatII G_ACGT'C
Cuts at: 0 518 1705 2127
Size: 518 1187 422
Fragments arranged by size:
1187 518 422
Acc65I G'GTAC_C
Cuts at: 0 1572 2127
Size: 1572 555
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 1179 1688 2006 2127
Size: 152 1027 509 318 121
Fragments arranged by size:
1027 509 318 152 121
AflII C'TTAA_G
Cuts at: 0 181 2127
Size: 181 1946
AflIII A'CryG_T
Cuts at: 0 580 1939 2127
Size: 580 1359 188
Fragments arranged by size:
1359 580 188
AhdI GACnn_n'nnGTC
Cuts at: 0 279 504 1254 2127
Size: 279 225 750 873
Fragments arranged by size:
873 750 279 225
AlwNI CAG_nnn'CTG
Cuts at: 0 289 2127
Size: 289 1838
ApaLI G'TGCA_C
Cuts at: 0 1540 2127
Size: 1540 587
ApoI r'AATT_y
Cuts at: 0 28 189 556 1770 2127
Size: 28 161 367 1214 357
Fragments arranged by size:
1214 367 357 161 28
AseI AT'TA_AT
Cuts at: 0 1357 2127
Size: 1357 770
BanI G'GyrC_C
Cuts at: 0 1572 2127
Size: 1572 555
BanII G_rGCy'C
Cuts at: 0 269 2127
Size: 269 1858
BbsI GAAGACnn'nnnn_
Cuts at: 0 1063 2127
Size: 1063 1064
BbvCI CC'TCA_GC
Cuts at: 0 100 2127
Size: 100 2027
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1531 1565 2127
Size: 1531 34 562
Fragments arranged by size:
1531 562 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 246 649 2127
Size: 246 403 1478
Fragments arranged by size:
1478 403 246
BclI T'GATC_A
Cuts at: 0 593 2127
Size: 593 1534
BglII A'GATC_T
Cuts at: 0 177 770 880 2127
Size: 177 593 110 1247
Fragments arranged by size:
1247 593 177 110
Bme1580I G_kGCm'C
Cuts at: 0 1476 1544 2127
Size: 1476 68 583
Fragments arranged by size:
1476 583 68
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 57 59 2127
Size: 57 2 2068
Fragments arranged by size:
2068 57 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 787 910 996 1204 1727 2127
Size: 787 123 86 208 523 400
Fragments arranged by size:
787 523 400 208 123 86
Bpu10I CC'TnA_GC
Cuts at: 0 100 352 2127
Size: 100 252 1775
Fragments arranged by size:
1775 252 100
BsaAI yAC'GTr
Cuts at: 0 1940 2127
Size: 1940 187
BsaBI GATnn'nnATC
Cuts at: 0 2045 2127
Size: 2045 82
BsaHI Gr'CG_yC
Cuts at: 0 515 1702 2127
Size: 515 1187 425
Fragments arranged by size:
1187 515 425
BsaWI w'CCGG_w
Cuts at: 0 60 817 2127
Size: 60 757 1310
Fragments arranged by size:
1310 757 60
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 412 442 2127
Size: 412 30 1685
Fragments arranged by size:
1685 412 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 372 718 1201 1229 1597 1645 2127
Size: 372 346 483 28 368 48 482
Fragments arranged by size:
483 482 372 368 346 48 28
BseYI C'CCAG_C
Cuts at: 0 406 1221 2127
Size: 406 815 906
Fragments arranged by size:
906 815 406
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 605 929 2127
Size: 421 184 324 1198
Fragments arranged by size:
1198 421 324 184
BsiHKAI G_wGCw'C
Cuts at: 0 269 1544 2127
Size: 269 1275 583
Fragments arranged by size:
1275 583 269
Bsp1286I G_dGCh'C
Cuts at: 0 269 1476 1544 2127
Size: 269 1207 68 583
Fragments arranged by size:
1207 583 269 68
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 62 2127
Size: 62 2065
BsrDI GCAATG_nn'
Cuts at: 0 1058 2127
Size: 1058 1069
BssSI C'ACGA_G
Cuts at: 0 1760 2127
Size: 1760 367
BstBI TT'CG_AA
Cuts at: 0 987 2127
Size: 987 1140
BstYI r'GATC_y
Cuts at: 0 177 608 770 880 2127
Size: 177 431 162 110 1247
Fragments arranged by size:
1247 431 177 162 110
BtgI C'CryG_G
Cuts at: 0 93 2127
Size: 93 2034
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1147 2127
Size: 1147 980
BtsI GCAGTG_nn'
Cuts at: 0 404 2127
Size: 404 1723
ClaI AT'CG_AT
Cuts at: 0 1361 2127
Size: 1361 766
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 195 230 1248 1283 1743 1778 2127
Size: 195 35 1018 35 460 35 349
Fragments arranged by size:
1018 460 349 195 35 35 35
DraI TTT'AAA
Cuts at: 0 1518 1986 2127
Size: 1518 468 141
Fragments arranged by size:
1518 468 141
DraIII CAC_nnn'GTG
Cuts at: 0 399 2127
Size: 399 1728
EaeI y'GGCC_r
Cuts at: 0 1013 1622 2127
Size: 1013 609 505
Fragments arranged by size:
1013 609 505
EcoICRI GAG'CTC
Cuts at: 0 267 2127
Size: 267 1860
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 787 910 996 1179 1204 1688 1727
Size: 152 635 123 86 183 25 484 39
Cuts at: 1727 2006 2127
Size: 279 121
Fragments arranged by size:
635 484 279 183 152 123 121 86
39 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 529 964 2127
Size: 529 435 1163
Fragments arranged by size:
1163 529 435
EcoO109I rG'GnC_Cy
Cuts at: 0 642 1152 2127
Size: 642 510 975
Fragments arranged by size:
975 642 510
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 525 929 2127
Size: 525 404 1198
Fragments arranged by size:
1198 525 404
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 265 297 490 522 1240 1272 1335 1354
Size: 265 32 193 32 718 32 63 19
Cuts at: 1354 1367 1386 1430 1462 1774 1806 2055 2087
Size: 13 19 44 32 312 32 249 32
Cuts at: 2087 2127
Size: 40
Fragments arranged by size:
718 312 265 249 193 63 44 40
32 32 32 32 32 32 19 19
13
HincII GTy'rAC
Cuts at: 0 1503 2127
Size: 1503 624
HindIII A'AGCT_T
Cuts at: 0 164 2127
Size: 164 1963
KpnI G_GTAC'C
Cuts at: 0 1576 2127
Size: 1576 551
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 432 1062 2127
Size: 432 630 1065
Fragments arranged by size:
1065 630 432
MscI TGG'CCA
Cuts at: 0 1015 2127
Size: 1015 1112
MslI CAynn'nnrTG
Cuts at: 0 905 1021 2127
Size: 905 116 1106
Fragments arranged by size:
1106 905 116
MspA1I CmG'CkG
Cuts at: 0 95 472 793 2127
Size: 95 377 321 1334
Fragments arranged by size:
1334 377 321 95
PfoI T'CCnGG_A
Cuts at: 0 248 1181 2127
Size: 248 933 946
Fragments arranged by size:
946 933 248
PmlI CAC'GTG
Cuts at: 0 1940 2127
Size: 1940 187
PpuMI rG'GwC_Cy
Cuts at: 0 1152 2127
Size: 1152 975
PstI C_TGCA'G
Cuts at: 0 1070 2127
Size: 1070 1057
PvuII CAG'CTG
Cuts at: 0 472 793 2127
Size: 472 321 1334
Fragments arranged by size:
1334 472 321
SacI G_AGCT'C
Cuts at: 0 269 2127
Size: 269 1858
SacII CC_GC'GG
Cuts at: 0 96 2127
Size: 96 2031
SfcI C'TryA_G
Cuts at: 0 1066 2127
Size: 1066 1061
SmlI C'TyrA_G
Cuts at: 0 181 2127
Size: 181 1946
SspI AAT'ATT
Cuts at: 0 948 2127
Size: 948 1179
StyI C'CwwG_G
Cuts at: 0 14 992 1568 2127
Size: 14 978 576 559
Fragments arranged by size:
978 576 559 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 839 1265 1351 2127
Size: 839 426 86 776
Fragments arranged by size:
839 776 426 86
TatI w'GTAC_w
Cuts at: 0 885 2127
Size: 885 1242
Tth111I GACn'n_nGTC
Cuts at: 0 849 1173 2127
Size: 849 324 954
Fragments arranged by size:
954 849 324
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1145 2127
Size: 1145 982
XmnI GAAnn'nnTTC
Cuts at: 0 1872 2127
Size: 1872 255
ZraI GAC'GTC
Cuts at: 0 516 1703 2127
Size: 516 1187 424
Fragments arranged by size:
1187 516 424
Enzymes that do cut:
AatII Acc65I AcuI AflII AflIII AhdI AlwNI ApaLI
ApoI AseI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglII Bme1580I BplI BpmI Bpu10I BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrDI BssSI BstBI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII EaeI EcoICRI Eco57MI EcoNI
EcoO109I FalI Hin4I HincII HindIII KpnI MmeI MscI
MslI MspA1I PfoI PmlI PpuMI PstI PvuII SacI
SacII SfcI SmlI SspI StyI TaqII TatI Tth111I
XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AgeI AleI AlfI AloI
ApaI AscI AsiSI AvaI AvrII BaeI BamHI BbeI
BfrBI BglI BlpI BmgBI BmrI BmtI BpuEI BsaI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BstAPI BstEII BstXI BstZ17I Bsu36I DrdI
EagI EarI EciI EcoRI EcoRV FseI FspI FspAI
HaeII HpaI KasI MfeI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI TstI XbaI XhoI
XmaI