(Circular) (Six-base) MAPSORT of: pFN21AB3158.seq Check: 2611 from: 1 to: 7477 pFN21AB3158 7477 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5287 278 Size: 53 83 186 936 3751 2468 Fragments arranged by size: 3751 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2014 4233 4820 1937 Size: 77 2219 587 4594 Fragments arranged by size: 4594 2219 587 77 Acc65I G'GTAC_C Cuts at: 4798 4798 Size: 7477 AclI AA'CG_TT Cuts at: 1563 5604 5977 1563 Size: 4041 373 3063 Fragments arranged by size: 4041 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2074 2263 2334 2476 3838 4291 5540 6924 Size: 442 189 71 142 1362 453 1249 1384 Cuts at: 6924 7192 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1362 1249 453 442 268 189 142 71 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7280 AflIII A'CryG_T Cuts at: 1203 6376 7353 1203 Size: 5173 977 1327 Fragments arranged by size: 5173 1327 977 AgeI A'CCGG_T Cuts at: 2249 6308 2249 Size: 4059 3418 AhdI GACnn_n'nnGTC Cuts at: 2227 6206 2227 Size: 3979 3498 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7477 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7477 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3075 3107 3075 Size: 32 7445 AlwNI CAG_nnn'CTG Cuts at: 3920 5220 6792 3920 Size: 1300 1572 4605 Fragments arranged by size: 4605 1572 1300 ApaLI G'TGCA_C Cuts at: 5534 6690 5534 Size: 1156 6321 ApoI r'AATT_y Cuts at: 1493 3690 4696 4786 5012 5164 7273 1493 Size: 2197 1006 90 226 152 2109 1697 Fragments arranged by size: 2197 2109 1697 1006 226 152 90 AseI AT'TA_AT Cuts at: 160 1032 2646 2862 4512 6031 160 Size: 872 1614 216 1650 1519 1606 Fragments arranged by size: 1650 1614 1606 1519 872 216 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7477 AvaI C'yCGr_G Cuts at: 1870 1943 2099 2292 4412 4802 1870 Size: 73 156 193 2120 390 4545 Fragments arranged by size: 4545 2120 390 193 156 73 AvrII C'CTAG_G Cuts at: 4292 4292 Size: 7477 BamHI G'GATC_C Cuts at: 4807 5174 4807 Size: 367 7110 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2930 4798 6253 618 Size: 325 217 627 1143 1868 1455 1842 Fragments arranged by size: 1868 1842 1455 1143 627 325 217 BanII G_rGCy'C Cuts at: 1391 4374 4796 1391 Size: 2983 422 4072 Fragments arranged by size: 4072 2983 422 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6506 BbvCI CC'TCA_GC Cuts at: 2799 4004 2799 Size: 1205 6272 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5668 5702 1903 Size: 34 3731 34 3678 Fragments arranged by size: 3731 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5369 6579 5369 Size: 1210 6267 BclI T'GATC_A Cuts at: 1548 4399 1548 Size: 2851 4626 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3797 6088 136 Size: 107 122 71 631 605 2125 2291 1525 Fragments arranged by size: 2291 2125 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2562 7472 2562 Size: 4910 2567 BlpI GC'TnA_GC Cuts at: 4894 4894 Size: 7477 Bme1580I G_kGCm'C Cuts at: 1790 2933 5538 6694 1790 Size: 1143 2605 1156 2573 Fragments arranged by size: 2605 2573 1156 1143 BmgBI CAC'GTC Cuts at: 1318 2701 1318 Size: 1383 6094 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5208 6166 442 Size: 416 53 83 399 24 3791 958 1753 Fragments arranged by size: 3791 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7477 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3583 3583 Size: 7477 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3075 3116 6137 1239 Size: 1836 41 3021 2579 Fragments arranged by size: 3021 2579 1836 41 Bpu10I CC'TnA_GC Cuts at: 2355 2799 4004 2355 Size: 444 1205 5828 Fragments arranged by size: 5828 1205 444 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5602 6467 6765 5602 Size: 865 298 6314 Fragments arranged by size: 6314 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2817 3386 6140 882 Size: 617 1318 569 2754 2219 Fragments arranged by size: 2754 2219 1318 617 569 BsaAI yAC'GTr Cuts at: 493 1204 2314 5268 493 Size: 711 1110 2954 2702 Fragments arranged by size: 2954 2702 1110 711 BsaBI GATnn'nnATC Cuts at: 2516 7471 2516 Size: 4955 2522 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2011 5284 5666 6301 Size: 53 83 186 936 478 3273 382 635 Cuts at: 6301 275 Size: 1451 Fragments arranged by size: 3273 1451 936 635 478 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2249 5910 6308 6582 6729 7140 2249 Size: 3661 398 274 147 411 2586 Fragments arranged by size: 3661 2586 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2363 2393 3075 3105 4273 Size: 30 165 30 960 30 682 30 1168 Cuts at: 4273 4303 1178 Size: 30 4352 Fragments arranged by size: 4352 1168 960 682 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2419 2422 2720 3632 4081 4131 4535 1186 Size: 1233 3 298 912 449 50 404 4128 Fragments arranged by size: 4128 1233 912 449 404 298 50 3 BseYI C'CCAG_C Cuts at: 6680 7176 6680 Size: 496 6981 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2327 1163 Size: 573 591 6313 Fragments arranged by size: 6313 591 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4927 5688 6716 1540 Size: 275 186 2926 761 1028 2301 Fragments arranged by size: 2926 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2166 5538 5623 6694 2166 Size: 3372 85 1071 2949 Fragments arranged by size: 3372 2949 1071 85 BsmI GAATG_Cn' Cuts at: 2923 4258 4988 5081 2923 Size: 1335 730 93 5319 Fragments arranged by size: 5319 1335 730 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2166 2933 4374 4796 5538 5623 6694 Size: 399 376 767 1441 422 742 85 1071 Cuts at: 6694 1391 Size: 2174 Fragments arranged by size: 2174 1441 1071 767 742 422 399 376 85 BspEI T'CCGG_A Cuts at: 7140 7140 Size: 7477 BspHI T'CATG_A Cuts at: 5366 7096 5366 Size: 1730 5747 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2058 2196 4832 844 Size: 1214 138 2636 3489 Fragments arranged by size: 3489 2636 1214 138 BsrBI CCG'CTC Cuts at: 1394 1823 2331 2412 5364 1394 Size: 429 508 81 2952 3507 Fragments arranged by size: 3507 2952 508 429 81 BsrDI GCAATG_nn' Cuts at: 65 1248 3273 4349 5972 6146 65 Size: 1183 2025 1076 1623 174 1396 Fragments arranged by size: 2025 1623 1396 1183 1076 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2249 6121 6308 1700 Size: 110 439 3872 187 2869 Fragments arranged by size: 3872 2869 439 187 110 BsrGI T'GTAC_A Cuts at: 96 2130 96 Size: 2034 5443 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7477 BssSI C'ACGA_G Cuts at: 5537 6549 5537 Size: 1012 6465 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7477 BstBI TT'CG_AA Cuts at: 6281 6281 Size: 7477 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7477 BstXI CCAn_nnnn'nTGG Cuts at: 4435 4630 4435 Size: 195 7282 BstYI r'GATC_y Cuts at: 1972 2562 3295 4807 5174 5559 5576 7028 7114 Size: 590 733 1512 367 385 17 1452 86 Cuts at: 7114 7126 7472 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1512 1452 733 590 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2481 2481 Size: 7477 BtgI C'CryG_G Cuts at: 513 1065 2004 2154 2680 513 Size: 552 939 150 526 5310 Fragments arranged by size: 5310 939 552 526 150 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4361 487 Size: 683 56 3135 3603 Fragments arranged by size: 3603 3135 683 56 BtsI GCAGTG_nn' Cuts at: 2836 3153 4993 5787 5807 2836 Size: 317 1840 794 20 4506 Fragments arranged by size: 4506 1840 794 317 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7477 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2805 2840 529 Size: 35 2241 35 5166 Fragments arranged by size: 5166 2241 35 35 DraI TTT'AAA Cuts at: 4781 4781 Size: 7477 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6484 809 Size: 507 5168 1802 Fragments arranged by size: 5168 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2178 2305 2607 Size: 54 1425 50 275 18 348 127 302 Cuts at: 2607 4924 5813 7442 8 Size: 2317 889 1629 43 Fragments arranged by size: 2317 1629 1425 889 348 302 275 127 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4924 1537 Size: 275 3112 4090 Fragments arranged by size: 4090 3112 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2081 2885 3552 3717 4279 4338 5407 1353 Size: 728 804 667 165 562 59 1069 3423 Fragments arranged by size: 3423 1069 804 728 667 562 165 59 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6064 6438 6584 387 Size: 1064 138 4475 374 146 1280 Fragments arranged by size: 4475 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2074 2263 2334 2476 3075 3116 3838 Size: 393 442 189 71 142 599 41 722 Cuts at: 3838 4291 5540 6137 6924 7192 1239 Size: 453 1249 597 787 268 1524 Fragments arranged by size: 1524 1249 787 722 599 597 453 442 393 268 189 142 71 41 EcoNI CCTnn'n_nnAGG Cuts at: 2141 2180 2480 2141 Size: 39 300 7138 Fragments arranged by size: 7138 300 39 EcoO109I rG'GnC_Cy Cuts at: 2084 2185 2374 2575 3647 4289 2084 Size: 101 189 201 1072 642 5272 Fragments arranged by size: 5272 1072 642 201 189 101 EcoRI G'AATT_C Cuts at: 3690 4786 5164 3690 Size: 1096 378 6003 Fragments arranged by size: 6003 1096 378 EcoRV GAT'ATC Cuts at: 4126 4126 Size: 7477 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3512 3512 Size: 7477 FspI TGC'GCA Cuts at: 5983 5983 Size: 7477 HaeII r_GCGC'y Cuts at: 1678 6624 1678 Size: 4946 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2213 2245 2315 2347 2795 Size: 32 135 32 641 32 70 32 448 Cuts at: 2795 2827 2965 2997 3456 3488 3644 3676 3732 Size: 32 138 32 459 32 156 32 56 Cuts at: 3732 3764 4273 4305 6118 6150 6192 6224 1373 Size: 32 509 32 1813 32 42 32 2626 Fragments arranged by size: 2626 1813 641 509 459 448 156 138 135 70 56 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3265 3319 3760 4234 4821 5067 Size: 988 281 1327 54 441 474 587 246 Cuts at: 5067 669 Size: 3079 Fragments arranged by size: 3079 1327 988 587 474 441 281 246 54 HindIII A'AGCT_T Cuts at: 748 4612 748 Size: 3864 3613 HpaI GTT'AAC Cuts at: 3265 3319 5067 3265 Size: 54 1748 5675 Fragments arranged by size: 5675 1748 54 KpnI G_GTAC'C Cuts at: 4802 4802 Size: 7477 MfeI C'AATT_G Cuts at: 2869 2937 5076 2869 Size: 68 2139 5270 Fragments arranged by size: 5270 2139 68 MluI A'CGCG_T Cuts at: 7353 7353 Size: 7477 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3792 6591 6775 1305 Size: 2487 2799 184 2007 Fragments arranged by size: 2799 2487 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2307 2609 10 Size: 54 1425 343 475 302 4878 Fragments arranged by size: 4878 1425 475 343 302 54 MslI CAynn'nnrTG Cuts at: 518 1166 3042 3488 4247 4433 4640 5435 5794 Size: 648 1876 446 759 186 207 795 359 Cuts at: 5794 5953 518 Size: 159 2042 Fragments arranged by size: 2042 1876 795 759 648 446 359 207 186 159 MspA1I CmG'CkG Cuts at: 1606 2094 2156 2236 2945 3712 4893 5570 6718 Size: 488 62 80 709 767 1181 677 1148 Cuts at: 6718 6963 1606 Size: 245 2120 Fragments arranged by size: 2120 1181 1148 767 709 677 488 245 80 62 NcoI C'CATG_G Cuts at: 513 1065 2004 2680 513 Size: 552 939 676 5310 Fragments arranged by size: 5310 939 676 552 NdeI CA'TA_TG Cuts at: 387 4740 387 Size: 4353 3124 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7477 NotI GC'GGCC_GC Cuts at: 4924 4924 Size: 7477 NspI r_CATG'y Cuts at: 1132 1229 4835 6380 1132 Size: 97 3606 1545 2229 Fragments arranged by size: 3606 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 4683 1348 Size: 3335 4142 PciI A'CATG_T Cuts at: 6376 6376 Size: 7477 PflMI CCAn_nnn'nTGG Cuts at: 1108 2956 3887 1108 Size: 1848 931 4698 Fragments arranged by size: 4698 1848 931 PfoI T'CCnGG_A Cuts at: 2577 2577 Size: 7477 PmeI GTTT'AAAC Cuts at: 4781 4781 Size: 7477 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3075 3107 5546 5578 7059 7091 3075 Size: 32 2439 32 1481 32 3461 Fragments arranged by size: 3461 2439 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2084 2185 2575 3647 4289 2084 Size: 101 390 1072 642 5272 Fragments arranged by size: 5272 1072 642 390 101 PshAI GACnn'nnGTC Cuts at: 1613 2448 3828 1613 Size: 835 1380 5262 Fragments arranged by size: 5262 1380 835 PsiI TTA'TAA Cuts at: 5047 5047 Size: 7477 PstI C_TGCA'G Cuts at: 830 2212 4359 4829 830 Size: 1382 2147 470 3478 Fragments arranged by size: 3478 2147 1382 470 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7477 PvuII CAG'CTG Cuts at: 2094 2236 2945 3712 2094 Size: 142 709 767 5859 Fragments arranged by size: 5859 767 709 142 SacII CC_GC'GG Cuts at: 2157 2157 Size: 7477 SalI G'TCGA_C Cuts at: 1936 4232 4819 1936 Size: 2296 587 4594 Fragments arranged by size: 4594 2296 587 SanDI GG'GwC_CC Cuts at: 2084 2575 2084 Size: 491 6986 SbfI CC_TGCA'GG Cuts at: 4829 4829 Size: 7477 ScaI AGT'ACT Cuts at: 4185 5725 7437 4185 Size: 1540 1712 4225 Fragments arranged by size: 4225 1712 1540 SfcI C'TryA_G Cuts at: 826 1045 2041 2208 2922 3419 3600 4095 4355 Size: 219 996 167 714 497 181 495 260 Cuts at: 4355 4825 5960 6641 6832 826 Size: 470 1135 681 191 1471 Fragments arranged by size: 1471 1135 996 714 681 497 495 470 260 219 191 181 167 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7477 SgrAI Cr'CCGG_yG Cuts at: 1700 2249 1700 Size: 549 6928 SmaI CCC'GGG Cuts at: 1872 2294 4804 1872 Size: 422 2510 4545 Fragments arranged by size: 4545 2510 422 SmlI C'TyrA_G Cuts at: 820 1017 1943 5581 6482 6744 820 Size: 197 926 3638 901 262 1553 Fragments arranged by size: 3638 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5268 493 Size: 4775 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7477 SphI G_CATG'C Cuts at: 1132 4835 1132 Size: 3703 3774 SspI AAT'ATT Cuts at: 5 52 3383 4774 5401 5 Size: 47 3331 1391 627 2081 Fragments arranged by size: 3331 2081 1391 627 47 StuI AGG'CCT Cuts at: 1517 4297 1517 Size: 2780 4697 StyI C'CwwG_G Cuts at: 513 1065 2004 2680 4292 4916 513 Size: 552 939 676 1612 624 3074 Fragments arranged by size: 3074 1612 939 676 624 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2738 3586 3732 3790 5504 5521 5674 Size: 1255 1158 848 146 58 1714 17 153 Cuts at: 5674 5859 6304 325 Size: 185 445 1498 Fragments arranged by size: 1714 1498 1255 1158 848 445 185 153 146 58 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2130 3735 4183 Size: 274 80 33 51 1443 153 1605 448 Cuts at: 4183 4705 5723 7435 96 Size: 522 1018 1712 138 Fragments arranged by size: 1712 1605 1443 1018 522 448 274 153 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2363 2395 7437 7469 2363 Size: 32 5042 32 2371 Fragments arranged by size: 5042 2371 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3680 4460 1316 Size: 2364 780 4333 Fragments arranged by size: 4333 2364 780 XbaI T'CTAG_A Cuts at: 4120 4813 4120 Size: 693 6784 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3403 3403 Size: 7477 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7477 XmaI C'CCGG_G Cuts at: 1870 2292 4802 1870 Size: 422 2510 4545 Fragments arranged by size: 4545 2510 422 XmnI GAAnn'nnTTC Cuts at: 3876 5606 3876 Size: 1730 5747 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5285 276 Size: 53 83 186 936 3751 2468 Fragments arranged by size: 3751 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI