(Circular) (Six-base) MAPSORT of: pFN21AB3044.seq Check: 3985 from: 1 to: 6577 pFN21AB3044 6577 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4387 278 Size: 53 83 186 936 2851 2468 Fragments arranged by size: 2851 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2389 2521 3291 3920 1937 Size: 452 132 770 629 4594 Fragments arranged by size: 4594 770 629 452 132 Acc65I G'GTAC_C Cuts at: 3898 3898 Size: 6577 AclI AA'CG_TT Cuts at: 1563 3826 4704 5077 1563 Size: 2263 878 373 3063 Fragments arranged by size: 3063 2263 878 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2196 4640 6024 6292 1632 Size: 564 2444 1384 268 1917 Fragments arranged by size: 2444 1917 1384 564 268 AfeI AGC'GCT Cuts at: 2081 2081 Size: 6577 AflII C'TTAA_G Cuts at: 820 1017 2897 820 Size: 197 1880 4500 Fragments arranged by size: 4500 1880 197 AflIII A'CryG_T Cuts at: 1203 2633 2692 5476 6453 1203 Size: 1430 59 2784 977 1327 Fragments arranged by size: 2784 1430 1327 977 59 AgeI A'CCGG_T Cuts at: 5408 5408 Size: 6577 AhdI GACnn_n'nnGTC Cuts at: 3374 5306 3374 Size: 1932 4645 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6577 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6577 AlwNI CAG_nnn'CTG Cuts at: 4320 5892 4320 Size: 1572 5005 ApaLI G'TGCA_C Cuts at: 4634 5790 4634 Size: 1156 5421 ApoI r'AATT_y Cuts at: 1493 3815 3886 4112 4264 6373 1493 Size: 2322 71 226 152 2109 1697 Fragments arranged by size: 2322 2109 1697 226 152 71 AseI AT'TA_AT Cuts at: 160 1032 2712 2955 5131 160 Size: 872 1680 243 2176 1606 Fragments arranged by size: 2176 1680 1606 872 243 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6577 AvaI C'yCGr_G Cuts at: 1870 1943 3902 1870 Size: 73 1959 4545 Fragments arranged by size: 4545 1959 73 BamHI G'GATC_C Cuts at: 2047 2204 2305 3328 3907 4274 2047 Size: 157 101 1023 579 367 4350 Fragments arranged by size: 4350 1023 579 367 157 101 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3769 3898 5353 618 Size: 325 217 627 1982 129 1455 1842 Fragments arranged by size: 1982 1842 1455 627 325 217 129 BanII G_rGCy'C Cuts at: 1391 2039 3896 1391 Size: 648 1857 4072 Fragments arranged by size: 4072 1857 648 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3132 928 Size: 971 1233 4373 Fragments arranged by size: 4373 1233 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4768 4802 1903 Size: 34 2831 34 3678 Fragments arranged by size: 3678 2831 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3819 4469 5679 3819 Size: 650 1210 4717 Fragments arranged by size: 4717 1210 650 BclI T'GATC_A Cuts at: 1548 1548 Size: 6577 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5188 136 Size: 107 122 71 631 605 3516 1525 Fragments arranged by size: 3516 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6572 6572 Size: 6577 BlpI GC'TnA_GC Cuts at: 3994 3994 Size: 6577 Bme1580I G_kGCm'C Cuts at: 1790 3772 4638 5794 1790 Size: 1982 866 1156 2573 Fragments arranged by size: 2573 1982 1156 866 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6577 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3424 4308 5266 Size: 416 53 83 399 24 2007 884 958 Cuts at: 5266 442 Size: 1753 Fragments arranged by size: 2007 1753 958 884 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6577 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2439 3090 3780 5237 1239 Size: 1200 651 690 1457 2579 Fragments arranged by size: 2579 1457 1200 690 651 Bpu10I CC'TnA_GC Cuts at: 2301 2301 Size: 6577 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4702 5567 5865 4702 Size: 865 298 5414 Fragments arranged by size: 5414 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5240 882 Size: 617 3741 2219 Fragments arranged by size: 3741 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2477 4368 493 Size: 711 1273 1891 2702 Fragments arranged by size: 2702 1891 1273 711 BsaBI GATnn'nnATC Cuts at: 2310 2368 2374 6571 2310 Size: 58 6 4197 2316 Fragments arranged by size: 4197 2316 58 6 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3360 4384 4766 5401 Size: 53 83 186 936 1827 1024 382 635 Cuts at: 5401 275 Size: 1451 Fragments arranged by size: 1827 1451 1024 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5010 5408 5682 5829 6240 5010 Size: 398 274 147 411 5347 Fragments arranged by size: 5347 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6352 Fragments arranged by size: 6352 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2057 3609 1186 Size: 871 1552 4154 Fragments arranged by size: 4154 1552 871 BseYI C'CCAG_C Cuts at: 5780 6276 5780 Size: 496 6081 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3227 1163 Size: 573 1491 4513 Fragments arranged by size: 4513 1491 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4027 4788 5816 1540 Size: 275 186 2026 761 1028 2301 Fragments arranged by size: 2301 2026 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2302 3619 4638 4723 5794 2302 Size: 1317 1019 85 1071 3085 Fragments arranged by size: 3085 1317 1071 1019 85 BsmI GAATG_Cn' Cuts at: 4088 4181 4088 Size: 93 6484 BsmBI CGTCTCn'nnnn_ Cuts at: 2048 3510 2048 Size: 1462 5115 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2039 2302 3619 3772 3896 4638 4723 Size: 399 249 263 1317 153 124 742 85 Cuts at: 4723 5794 1391 Size: 1071 2174 Fragments arranged by size: 2174 1317 1071 742 399 263 249 153 124 85 BspEI T'CCGG_A Cuts at: 6240 6240 Size: 6577 BspHI T'CATG_A Cuts at: 3607 4466 6196 3607 Size: 859 1730 3988 Fragments arranged by size: 3988 1730 859 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3932 844 Size: 3088 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4464 1394 Size: 429 2641 3507 Fragments arranged by size: 3507 2641 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2330 2892 3415 5072 5246 65 Size: 1183 1082 562 523 1657 174 1396 Fragments arranged by size: 1657 1396 1183 1082 562 523 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5221 5408 1700 Size: 110 3411 187 2869 Fragments arranged by size: 3411 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6577 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6577 BssSI C'ACGA_G Cuts at: 4637 5649 4637 Size: 1012 5565 BstAPI GCAn_nnn'nTGC Cuts at: 794 2759 794 Size: 1965 4612 BstBI TT'CG_AA Cuts at: 3541 5381 3541 Size: 1840 4737 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6577 BstYI r'GATC_y Cuts at: 1972 2047 2204 2305 2369 3328 3907 4274 4659 Size: 75 157 101 64 959 579 367 385 Cuts at: 4659 4676 6128 6214 6226 6572 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 959 579 385 367 346 157 101 86 75 64 17 12 BstZ17I GTA'TAC Cuts at: 3292 3292 Size: 6577 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6025 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5838 Fragments arranged by size: 5838 683 56 BtsI GCAGTG_nn' Cuts at: 3798 4093 4887 4907 3798 Size: 295 794 20 5468 Fragments arranged by size: 5468 794 295 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6577 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6542 DraI TTT'AAA Cuts at: 2728 2749 3336 3881 2728 Size: 21 587 545 5424 Fragments arranged by size: 5424 587 545 21 DraIII CAC_nnn'GTG Cuts at: 3029 3029 Size: 6577 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5584 809 Size: 507 4268 1802 Fragments arranged by size: 4268 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4024 4913 6542 Size: 54 1425 50 275 18 2194 889 1629 Cuts at: 6542 8 Size: 43 Fragments arranged by size: 2194 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4024 1537 Size: 275 2212 4090 Fragments arranged by size: 4090 2212 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2455 2822 2958 3133 3192 3292 4507 1353 Size: 1102 367 136 175 59 100 1215 3423 Fragments arranged by size: 3423 1215 1102 367 175 136 100 59 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3258 5164 5538 5684 387 Size: 1064 138 1669 1906 374 146 1280 Fragments arranged by size: 1906 1669 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2196 2439 3090 3780 4640 5237 6024 Size: 393 564 243 651 690 860 597 787 Cuts at: 6024 6292 1239 Size: 268 1524 Fragments arranged by size: 1524 860 787 690 651 597 564 393 268 243 EcoO109I rG'GnC_Cy Cuts at: 2089 2939 2089 Size: 850 5727 EcoRI G'AATT_C Cuts at: 3886 4264 3886 Size: 378 6199 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3008 3008 Size: 6577 FspI TGC'GCA Cuts at: 5083 5083 Size: 6577 HaeII r_GCGC'y Cuts at: 1678 2083 5724 1678 Size: 405 3641 2531 Fragments arranged by size: 3641 2531 405 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2045 2077 2193 2225 2555 Size: 32 135 32 473 32 116 32 330 Cuts at: 2555 2587 3360 3392 5218 5250 5292 5324 1373 Size: 32 773 32 1826 32 42 32 2626 Fragments arranged by size: 2626 1826 773 473 330 135 116 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2390 2530 3400 3921 4167 669 Size: 988 281 452 140 870 521 246 3079 Fragments arranged by size: 3079 988 870 521 452 281 246 140 HindIII A'AGCT_T Cuts at: 748 748 Size: 6577 HpaI GTT'AAC Cuts at: 4167 4167 Size: 6577 KpnI G_GTAC'C Cuts at: 3902 3902 Size: 6577 MfeI C'AATT_G Cuts at: 3253 4176 3253 Size: 923 5654 MluI A'CGCG_T Cuts at: 2633 6453 2633 Size: 3820 2757 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1989 5691 5875 1305 Size: 684 3702 184 2007 Fragments arranged by size: 3702 2007 684 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4755 Fragments arranged by size: 4755 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3612 4535 4894 5053 518 Size: 648 2446 923 359 159 2042 Fragments arranged by size: 2446 2042 923 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2820 3993 4670 5818 6063 1606 Size: 1214 1173 677 1148 245 2120 Fragments arranged by size: 2120 1214 1173 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6025 NdeI CA'TA_TG Cuts at: 387 3739 387 Size: 3352 3225 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6577 NotI GC'GGCC_GC Cuts at: 4024 4024 Size: 6577 NspI r_CATG'y Cuts at: 1132 1229 2696 3935 5480 1132 Size: 97 1467 1239 1545 2229 Fragments arranged by size: 2229 1545 1467 1239 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6577 PciI A'CATG_T Cuts at: 2692 5476 2692 Size: 2784 3793 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6577 PmeI GTTT'AAAC Cuts at: 3881 3881 Size: 6577 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4646 4678 6159 6191 4646 Size: 32 1481 32 5032 Fragments arranged by size: 5032 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2089 2089 Size: 6577 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6577 PsiI TTA'TAA Cuts at: 4147 4147 Size: 6577 PstI C_TGCA'G Cuts at: 830 2201 3859 3929 830 Size: 1371 1658 70 3478 Fragments arranged by size: 3478 1658 1371 70 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6577 PvuII CAG'CTG Cuts at: 2820 2820 Size: 6577 SalI G'TCGA_C Cuts at: 1936 2388 3919 1936 Size: 452 1531 4594 Fragments arranged by size: 4594 1531 452 SapI GCTCTTCn'nnn_ Cuts at: 3133 3133 Size: 6577 SbfI CC_TGCA'GG Cuts at: 3929 3929 Size: 6577 ScaI AGT'ACT Cuts at: 2546 4825 6537 2546 Size: 2279 1712 2586 Fragments arranged by size: 2586 2279 1712 SfcI C'TryA_G Cuts at: 826 1045 2197 2537 3118 3426 3855 3925 5060 Size: 219 1152 340 581 308 429 70 1135 Cuts at: 5060 5741 5932 826 Size: 681 191 1471 Fragments arranged by size: 1471 1152 1135 681 581 429 340 308 219 191 70 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6577 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6577 SmaI CCC'GGG Cuts at: 1872 3904 1872 Size: 2032 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2897 4681 5582 5844 820 Size: 197 926 954 1784 901 262 1553 Fragments arranged by size: 1784 1553 954 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4368 493 Size: 3875 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6577 SphI G_CATG'C Cuts at: 1132 3935 1132 Size: 2803 3774 SspI AAT'ATT Cuts at: 5 52 4501 5 Size: 47 4449 2081 Fragments arranged by size: 4449 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6577 StyI C'CwwG_G Cuts at: 513 1065 3675 4016 513 Size: 552 2610 341 3074 Fragments arranged by size: 3074 2610 552 341 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2109 4604 4621 4774 4959 5404 325 Size: 1255 529 2495 17 153 185 445 1498 Fragments arranged by size: 2495 1498 1255 529 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2544 4823 6535 Size: 274 80 33 51 1443 567 2279 1712 Cuts at: 6535 96 Size: 138 Fragments arranged by size: 2279 1712 1443 567 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6537 6569 6537 Size: 32 6545 Tth111I GACn'n_nGTC Cuts at: 1316 2059 1316 Size: 743 5834 XbaI T'CTAG_A Cuts at: 3913 3913 Size: 6577 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3259 3259 Size: 6577 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6577 XmaI C'CCGG_G Cuts at: 1870 3902 1870 Size: 2032 4545 XmnI GAAnn'nnTTC Cuts at: 3435 4706 3435 Size: 1271 5306 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4385 276 Size: 53 83 186 936 2851 2468 Fragments arranged by size: 2851 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI