(Circular) (Six-base) MAPSORT of: pFN21AA2027.seq Check: 6590 from: 1 to: 7033 pFN21AA2027 7033 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2284 2414 2284 Size: 130 6903 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4056 4843 278 Size: 53 83 186 936 2520 787 2468 Fragments arranged by size: 2520 2468 936 787 186 83 53 AccI GT'mk_AC Cuts at: 1937 3206 3656 4376 1937 Size: 1269 450 720 4594 Fragments arranged by size: 4594 1269 720 450 Acc65I G'GTAC_C Cuts at: 4354 4354 Size: 7033 AclI AA'CG_TT Cuts at: 1563 5160 5533 1563 Size: 3597 373 3063 Fragments arranged by size: 3597 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2478 2682 3056 5096 6480 6748 1632 Size: 846 204 374 2040 1384 268 1917 Fragments arranged by size: 2040 1917 1384 846 374 268 204 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6836 AflIII A'CryG_T Cuts at: 1203 3302 5932 6909 1203 Size: 2099 2630 977 1327 Fragments arranged by size: 2630 2099 1327 977 AgeI A'CCGG_T Cuts at: 5864 5864 Size: 7033 AhdI GACnn_n'nnGTC Cuts at: 4111 5762 4111 Size: 1651 5382 AleI CACnn'nnGTG Cuts at: 1166 3096 1166 Size: 1930 5103 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7033 AlwNI CAG_nnn'CTG Cuts at: 3860 3959 3962 4776 6348 3860 Size: 99 3 814 1572 4545 Fragments arranged by size: 4545 1572 814 99 3 ApaI G_GGCC'C Cuts at: 2774 2774 Size: 7033 ApaLI G'TGCA_C Cuts at: 3179 5090 6246 3179 Size: 1911 1156 3966 Fragments arranged by size: 3966 1911 1156 ApoI r'AATT_y Cuts at: 1493 3464 4342 4568 4720 6829 1493 Size: 1971 878 226 152 2109 1697 Fragments arranged by size: 2109 1971 1697 878 226 152 AseI AT'TA_AT Cuts at: 160 1032 4172 5587 160 Size: 872 3140 1415 1606 Fragments arranged by size: 3140 1606 1415 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7033 AvaI C'yCGr_G Cuts at: 1870 1943 2341 2767 4358 1870 Size: 73 398 426 1591 4545 Fragments arranged by size: 4545 1591 426 398 73 BamHI G'GATC_C Cuts at: 3586 4363 4730 3586 Size: 777 367 5889 Fragments arranged by size: 5889 777 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2150 2854 4354 5809 618 Size: 325 217 627 363 704 1500 1455 1842 Fragments arranged by size: 1842 1500 1455 704 627 363 325 217 BanII G_rGCy'C Cuts at: 1391 2774 3120 4352 1391 Size: 1383 346 1232 4072 Fragments arranged by size: 4072 1383 1232 346 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2076 2232 3875 4311 928 Size: 971 177 156 1643 436 3650 Fragments arranged by size: 3650 1643 971 436 177 156 BbvCI CC'TCA_GC Cuts at: 2171 2171 Size: 7033 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5224 5258 1903 Size: 34 3287 34 3678 Fragments arranged by size: 3678 3287 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3029 4925 6135 3029 Size: 1896 1210 3927 Fragments arranged by size: 3927 1896 1210 BclI T'GATC_A Cuts at: 1548 2425 3568 1548 Size: 877 1143 5013 Fragments arranged by size: 5013 1143 877 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2778 5644 136 Size: 107 122 71 631 605 1106 2866 1525 Fragments arranged by size: 2866 1525 1106 631 605 122 107 71 BglII A'GATC_T Cuts at: 7028 7028 Size: 7033 BlpI GC'TnA_GC Cuts at: 4450 4450 Size: 7033 Bme1580I G_kGCm'C Cuts at: 1790 2155 2774 3161 3183 5094 6250 1790 Size: 365 619 387 22 1911 1156 2573 Fragments arranged by size: 2573 1911 1156 619 387 365 22 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7033 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3009 4764 5722 Size: 416 53 83 399 24 1592 1755 958 Cuts at: 5722 442 Size: 1753 Fragments arranged by size: 1755 1753 1592 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3137 1056 Size: 2081 4952 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2552 2552 Size: 7033 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2562 2592 3134 3268 3321 3832 3966 4007 Size: 1323 30 542 134 53 511 134 41 Cuts at: 4007 5693 1239 Size: 1686 2579 Fragments arranged by size: 2579 1686 1323 542 511 134 134 53 41 30 Bpu10I CC'TnA_GC Cuts at: 2171 3054 3818 3966 2171 Size: 883 764 148 5238 Fragments arranged by size: 5238 883 764 148 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3914 5158 6023 6321 3914 Size: 1244 865 298 4626 Fragments arranged by size: 4626 1244 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2213 3286 5696 882 Size: 617 714 1073 2410 2219 Fragments arranged by size: 2410 2219 1073 714 617 BsaAI yAC'GTr Cuts at: 493 1204 3303 4824 493 Size: 711 2099 1521 2702 Fragments arranged by size: 2702 2099 1521 711 BsaBI GATnn'nnATC Cuts at: 3419 7027 3419 Size: 3608 3425 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2532 4053 4288 4840 Size: 53 83 186 936 999 1521 235 552 Cuts at: 4840 5222 5857 275 Size: 382 635 1451 Fragments arranged by size: 1521 1451 999 936 635 552 382 235 186 83 53 BsaWI w'CCGG_w Cuts at: 2060 5466 5864 6138 6285 6696 2060 Size: 3406 398 274 147 411 2397 Fragments arranged by size: 3406 2397 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2103 2133 3926 3956 1178 Size: 30 165 30 700 30 1793 30 4255 Fragments arranged by size: 4255 1793 700 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3621 1186 Size: 2435 4598 BseYI C'CCAG_C Cuts at: 2304 2942 3294 3908 6236 6732 2304 Size: 638 352 614 2328 496 2605 Fragments arranged by size: 2605 2328 638 614 496 352 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2285 2621 2990 1163 Size: 573 549 336 369 5206 Fragments arranged by size: 5206 573 549 369 336 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4483 5244 6272 1540 Size: 275 186 2482 761 1028 2301 Fragments arranged by size: 2482 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3052 3120 3183 3779 5094 5179 6250 3052 Size: 68 63 596 1315 85 1071 3835 Fragments arranged by size: 3835 1315 1071 596 85 68 63 BsmI GAATG_Cn' Cuts at: 2137 4544 4637 2137 Size: 2407 93 4533 Fragments arranged by size: 4533 2407 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3007 3007 Size: 7033 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2155 2774 3052 3120 3161 3183 3779 Size: 399 365 619 278 68 41 22 596 Cuts at: 3779 4352 5094 5179 6250 1391 Size: 573 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 619 596 573 399 365 278 85 68 41 22 BspEI T'CCGG_A Cuts at: 2060 6696 2060 Size: 4636 2397 BspHI T'CATG_A Cuts at: 4922 6652 4922 Size: 1730 5303 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2194 2284 2414 3362 4388 844 Size: 1350 90 130 948 1026 3489 Fragments arranged by size: 3489 1350 1026 948 130 90 BsrBI CCG'CTC Cuts at: 1394 1823 4920 1394 Size: 429 3097 3507 Fragments arranged by size: 3507 3097 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5528 5702 65 Size: 1183 4280 174 1396 Fragments arranged by size: 4280 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5677 5864 1700 Size: 110 3867 187 2869 Fragments arranged by size: 3867 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7033 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7033 BssSI C'ACGA_G Cuts at: 5093 6105 5093 Size: 1012 6021 BstAPI GCAn_nnn'nTGC Cuts at: 794 3962 794 Size: 3168 3865 BstBI TT'CG_AA Cuts at: 5837 5837 Size: 7033 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7033 BstXI CCAn_nnnn'nTGG Cuts at: 3098 3161 3716 3098 Size: 63 555 6415 Fragments arranged by size: 6415 555 63 BstYI r'GATC_y Cuts at: 1972 2362 2996 3586 4202 4363 4730 5115 5132 Size: 390 634 590 616 161 367 385 17 Cuts at: 5132 6584 6670 6682 7028 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 634 616 590 390 385 367 346 161 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3633 3633 Size: 7033 BtgI C'CryG_G Cuts at: 513 1065 2004 3837 513 Size: 552 939 1833 3709 Fragments arranged by size: 3709 1833 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6294 Fragments arranged by size: 6294 683 56 BtsI GCAGTG_nn' Cuts at: 2760 2982 3024 3103 4549 5343 5363 2760 Size: 222 42 79 1446 794 20 4430 Fragments arranged by size: 4430 1446 794 222 79 42 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7033 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6998 DraI TTT'AAA Cuts at: 4337 4337 Size: 7033 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6040 809 Size: 507 4724 1802 Fragments arranged by size: 4724 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3508 4480 5369 Size: 54 1425 50 275 18 1678 972 889 Cuts at: 5369 6998 8 Size: 1629 43 Fragments arranged by size: 1678 1629 1425 972 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4480 1537 Size: 275 2668 4090 Fragments arranged by size: 4090 2668 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3342 4963 1353 Size: 1989 1621 3423 Fragments arranged by size: 3423 1989 1621 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5620 5994 6140 387 Size: 1064 138 4031 374 146 1280 Fragments arranged by size: 4031 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3118 3118 Size: 7033 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2478 2562 2592 2682 3056 3134 3268 Size: 393 846 84 30 90 374 78 134 Cuts at: 3268 3321 3832 3966 4007 5096 5693 6480 6748 Size: 53 511 134 41 1089 597 787 268 Cuts at: 6748 1239 Size: 1524 Fragments arranged by size: 1524 1089 846 787 597 511 393 374 268 134 134 90 84 78 53 41 30 EcoNI CCTnn'n_nnAGG Cuts at: 2201 2526 2201 Size: 325 6708 EcoO109I rG'GnC_Cy Cuts at: 2703 2771 3253 2703 Size: 68 482 6483 Fragments arranged by size: 6483 482 68 EcoRI G'AATT_C Cuts at: 4342 4720 4342 Size: 378 6655 FspI TGC'GCA Cuts at: 5539 5539 Size: 7033 HaeII r_GCGC'y Cuts at: 1678 6180 1678 Size: 4502 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2103 2135 2543 2575 2981 Size: 32 135 32 531 32 408 32 406 Cuts at: 2981 3013 3571 3603 3862 3894 4097 4129 5674 Size: 32 558 32 259 32 203 32 1545 Cuts at: 5674 5706 5748 5780 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1545 558 531 408 406 259 203 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3354 4377 4623 669 Size: 988 281 1416 1023 246 3079 Fragments arranged by size: 3079 1416 1023 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7033 HpaI GTT'AAC Cuts at: 4623 4623 Size: 7033 KpnI G_GTAC'C Cuts at: 4358 4358 Size: 7033 MfeI C'AATT_G Cuts at: 4632 4632 Size: 7033 MluI A'CGCG_T Cuts at: 6909 6909 Size: 7033 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3241 4309 6147 6331 1305 Size: 1936 1068 1838 184 2007 Fragments arranged by size: 2007 1936 1838 1068 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5211 Fragments arranged by size: 5211 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3096 4991 5350 5509 518 Size: 648 1930 1895 359 159 2042 Fragments arranged by size: 2042 1930 1895 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2186 2308 2860 3076 3194 3200 3734 3959 Size: 580 122 552 216 118 6 534 225 Cuts at: 3959 4027 4449 5126 6274 6519 1606 Size: 68 422 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 580 552 534 422 245 225 216 122 118 68 6 NcoI C'CATG_G Cuts at: 513 1065 2004 3837 513 Size: 552 939 1833 3709 Fragments arranged by size: 3709 1833 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7033 NheI G'CTAG_C Cuts at: 1052 3133 1052 Size: 2081 4952 NotI GC'GGCC_GC Cuts at: 4480 4480 Size: 7033 NruI TCG'CGA Cuts at: 4127 4127 Size: 7033 NspI r_CATG'y Cuts at: 1132 1229 2835 3432 4391 5936 1132 Size: 97 1606 597 959 1545 2229 Fragments arranged by size: 2229 1606 1545 959 597 97 PasI CC'CwG_GG Cuts at: 1348 2178 4117 1348 Size: 830 1939 4264 Fragments arranged by size: 4264 1939 830 PciI A'CATG_T Cuts at: 5932 5932 Size: 7033 PflMI CCAn_nnn'nTGG Cuts at: 1108 3112 3860 1108 Size: 2004 748 4281 Fragments arranged by size: 4281 2004 748 PfoI T'CCnGG_A Cuts at: 3982 3982 Size: 7033 PmeI GTTT'AAAC Cuts at: 4337 4337 Size: 7033 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2154 2186 5102 5134 6615 6647 2154 Size: 32 2916 32 1481 32 2540 Fragments arranged by size: 2916 2540 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2703 3253 2703 Size: 550 6483 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7033 PsiI TTA'TAA Cuts at: 4603 4603 Size: 7033 PspOMI G'GGCC_C Cuts at: 2770 2770 Size: 7033 PstI C_TGCA'G Cuts at: 830 2161 2353 3199 4278 4385 830 Size: 1331 192 846 1079 107 3478 Fragments arranged by size: 3478 1331 1079 846 192 107 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7033 PvuII CAG'CTG Cuts at: 2186 2308 3076 3194 3200 3734 3959 4027 2186 Size: 122 768 118 6 534 225 68 5192 Fragments arranged by size: 5192 768 534 225 122 118 68 6 SacI G_AGCT'C Cuts at: 3120 3120 Size: 7033 SalI G'TCGA_C Cuts at: 1936 4375 1936 Size: 2439 4594 SbfI CC_TGCA'GG Cuts at: 4385 4385 Size: 7033 ScaI AGT'ACT Cuts at: 5281 6993 5281 Size: 1712 5321 SexAI A'CCwGG_T Cuts at: 3244 3244 Size: 7033 SfcI C'TryA_G Cuts at: 826 1045 2157 2349 2649 3195 3207 4274 4381 Size: 219 1112 192 300 546 12 1067 107 Cuts at: 4381 5516 6197 6388 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1112 1067 681 546 300 219 192 191 107 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7033 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7033 SmaI CCC'GGG Cuts at: 1872 4360 1872 Size: 2488 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3929 5137 6038 6300 820 Size: 197 926 1986 1208 901 262 1553 Fragments arranged by size: 1986 1553 1208 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4824 493 Size: 4331 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7033 SphI G_CATG'C Cuts at: 1132 3432 4391 1132 Size: 2300 959 3774 Fragments arranged by size: 3774 2300 959 SspI AAT'ATT Cuts at: 5 52 4957 5 Size: 47 4905 2081 Fragments arranged by size: 4905 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7033 StyI C'CwwG_G Cuts at: 513 1065 2004 2776 3837 4101 4472 513 Size: 552 939 772 1061 264 371 3074 Fragments arranged by size: 3074 1061 939 772 552 371 264 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2240 3830 5060 5077 5230 5415 5860 Size: 1255 660 1590 1230 17 153 185 445 Cuts at: 5860 325 Size: 1498 Fragments arranged by size: 1590 1498 1255 1230 660 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5279 6991 96 Size: 274 80 33 51 1443 3302 1712 138 Fragments arranged by size: 3302 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4043 4075 6993 7025 4043 Size: 32 2918 32 4051 Fragments arranged by size: 4051 2918 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7033 XbaI T'CTAG_A Cuts at: 4369 4369 Size: 7033 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7033 XmaI C'CCGG_G Cuts at: 1870 4358 1870 Size: 2488 4545 XmnI GAAnn'nnTTC Cuts at: 5162 5162 Size: 7033 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4054 4841 276 Size: 53 83 186 936 2520 787 2468 Fragments arranged by size: 2520 2468 936 787 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI