Restriction map for
pFN21AA1887
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I ApaLI
AscI Asp700I Asp718I AspEI AviII
AvrII BbeI BciVI BclI BglII
BlnI BsaBI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I ClaI
CpoI EagI Eam1105I EclXI Eco52I
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NarI
NdeI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmeI
PshAI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 2435 GTCTAC GT^[AC][GT]AC
AcsI 2028 AAATTC [GA]^AATT[CT]
AfaI 339 GTAC GT^AC
AflII 2815 CTTAAG C^TTAAG
AflIII 2683 ACACGT A^C[GA][CT]GT
AocI 1337 CCTCAGG CC^T[ACGT]AGG
Aor51HI 77 AGCGCT AGC^GCT
ApoI 2028 AAATTC [GA]^AATT[CT]
AseI 2602 ATTAAT AT^TAAT
AsnI 2602 ATTAAT AT^TAAT
BbrPI 2684 CACGTG CAC^GTG
BcgI 2445 CGAGAGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2815 CTTAAG C^TTAAG
BlpI 2770 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2770 GCTGAGC GC^T[ACGT]AGC
BsaAI 2684 CACGTG [CT]AC^GT[GA]
Bsu36I 1337 CCTCAGG CC^T[ACGT]AGG
CelII 2770 GCTGAGC GC^T[ACGT]AGC
DraI 2025 TTTAAA TTT^AAA
Eco47III 77 AGCGCT AGC^GCT
Eco81I 1337 CCTCAGG CC^T[ACGT]AGG
EcoNI 2358 CCTCTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 77 AGCGCT [GA]GCGC^[CT]
HgaI 1089 GCGTC GACGC(5/10)
HindIII 1284 AAGCTT A^AGCTT
MaeII 2685 ACGT A^CGT
NaeI 2645 GCCGGC GCC^GGC
NcoI 2511 CCATGG C^CATGG
NgoMIV 2645 GCCGGC G^CCGGC
NheI 958 GCTAGC G^CTAGC
PmaCI 2684 CACGTG CAC^GTG
PmlI 2684 CACGTG CAC^GTG
PshBI 2602 ATTAAT AT^TAAT
RsaI 339 GTAC GT^AC
SacI 2345 GAGCTC GAGCT^C
SexAI 1746 ACCTGGT A^CC[AT]GGT
SgrAI 307 CACCGGCG C[GA]^CCGG[CT]G
SmlI 2815 CTTAAG C^T[CT][GA]AG
XcmI 1671 CCACAGTTTATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AspHI 100 GTGCTC G[AT]GC[AT]^C
2345 GAGCTC
AspI 571 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1745 GACCTGGTC
BalI 50 TGGCCA TGG^CCA
2310 TGGCCA
BamHI 2055 GGATCC G^GATCC
2781 GGATCC
BglI 1773 GCCTGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2475 GCCTGCCAGGC
BsiHKAI 100 GTGCTC G[AT]GC[AT]^C
2345 GAGCTC
BsrFI 308 ACCGGC [GA]^CCGG[CT]
2645 GCCGGC
BstEII 243 GGTGACC G^GT[ACGT]ACC
2236 GGTGACC
BstPI 243 GGTGACC G^GT[ACGT]ACC
2236 GGTGACC
BstXI 1272 CCACTCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2225 CCAGCCTGCTGG
CfoI 78 GCGC GCG^C
2035 GCGC
Cfr10I 308 ACCGGC [GA]^CCGG[CT]
2645 GCCGGC
DrdI 1154 GACGGAGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2524 GACCCCCCAGTC
EcoO65I 243 GGTGACC G^GT[ACGT]ACC
2236 GGTGACC
HhaI 78 GCGC GCG^C
2035 GCGC
HinP1I 78 GCGC G^CGC
2035 GCGC
MluNI 50 TGGCCA TGG^CCA
2310 TGGCCA
MscI 50 TGGCCA TGG^CCA
2310 TGGCCA
MslI 784 CACTGTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1273 CACTCTGATG
PflFI 571 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1745 GACCTGGTC
StuI 518 AGGCCT AGG^CCT
2482 AGGCCT
Tsp509I 1221 AATT ^AATT
2029 AATT
Tth111I 571 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1745 GACCTGGTC
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# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AluI AlwI AlwNI ApaI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StyI TaqI Tru9I TseI
Tsp45I TspRI TthHB8I Van91I XhoII
XmaCI XmaI