Restriction map for
pFN21AA1887
(Linear) (Six-base) MAPSORT of: KIAA1887.seq Check: 541 from: 1 to: 3009
KIAA1887.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 367 660 896 3009
Size: 367 293 236 2113
Fragments arranged by size:
2113 367 293 236
AccI GT'mk_AC
Cuts at: 0 2436 3009
Size: 2436 573
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 749 857 1796 1826 2780 3009
Size: 749 108 939 30 954 229
Fragments arranged by size:
954 939 749 229 108 30
AfeI AGC'GCT
Cuts at: 0 79 3009
Size: 79 2930
AflII C'TTAA_G
Cuts at: 0 2815 3009
Size: 2815 194
AflIII A'CryG_T
Cuts at: 0 2683 3009
Size: 2683 326
AleI CACnn'nnGTG
Cuts at: 0 788 3009
Size: 788 2221
AlwNI CAG_nnn'CTG
Cuts at: 0 541 1578 2476 2536 3009
Size: 541 1037 898 60 473
Fragments arranged by size:
1037 898 541 473 60
ApaI G_GGCC'C
Cuts at: 0 46 425 908 921 1173 1897 2801 2964
Size: 46 379 483 13 252 724 904 163
Cuts at: 2964 3009
Size: 45
Fragments arranged by size:
904 724 483 379 252 163 46 45
13
ApoI r'AATT_y
Cuts at: 0 2028 3009
Size: 2028 981
AseI AT'TA_AT
Cuts at: 0 2603 3009
Size: 2603 406
AvaI C'yCGr_G
Cuts at: 0 249 819 1166 2064 2148 2443 2624 2794
Size: 249 570 347 898 84 295 181 170
Cuts at: 2794 2826 2933 2957 3009
Size: 32 107 24 52
Fragments arranged by size:
898 570 347 295 249 181 170 107
84 52 32 24
BamHI G'GATC_C
Cuts at: 0 2055 2781 3009
Size: 2055 726 228
Fragments arranged by size:
2055 726 228
BanI G'GyrC_C
Cuts at: 0 711 942 1047 1361 1420 2221 2820 3009
Size: 711 231 105 314 59 801 599 189
Fragments arranged by size:
801 711 599 314 231 189 105 59
BanII G_rGCy'C
Cuts at: 0 46 394 425 730 908 921 1173 1348
Size: 46 348 31 305 178 13 252 175
Cuts at: 1348 1724 1897 2271 2349 2452 2777 2801 2964
Size: 376 173 374 78 103 325 24 163
Cuts at: 2964 3009
Size: 45
Fragments arranged by size:
376 374 348 325 305 252 178 175
173 163 103 78 46 45 31 24
13
BbsI GAAGACnn'nnnn_
Cuts at: 0 125 863 2237 3009
Size: 125 738 1374 772
Fragments arranged by size:
1374 772 738 125
BbvCI CC'TCA_GC
Cuts at: 0 163 1053 1405 2457 3009
Size: 163 890 352 1052 552
Fragments arranged by size:
1052 890 552 352 163
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2434 2468 3009
Size: 2434 34 541
Fragments arranged by size:
2434 541 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1779 2481 3009
Size: 1779 702 528
Fragments arranged by size:
1779 702 528
BlpI GC'TnA_GC
Cuts at: 0 2771 3009
Size: 2771 238
Bme1580I G_kGCm'C
Cuts at: 0 46 425 716 908 921 945 1044 1173
Size: 46 379 291 192 13 24 99 129
Cuts at: 1173 1425 1897 2100 2801 2825 2964 3009
Size: 252 472 203 701 24 139 45
Fragments arranged by size:
701 472 379 291 252 203 192 139
129 99 46 45 24 24 13
BmrI ACTGGGnnnn_n'
Cuts at: 0 568 693 1447 1504 1703 2524 2558 3009
Size: 568 125 754 57 199 821 34 451
Fragments arranged by size:
821 754 568 451 199 125 57 34
BmtI G_CTAG'C
Cuts at: 0 962 3009
Size: 962 2047
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 712 1157 1181 3009
Size: 712 445 24 1828
Fragments arranged by size:
1828 712 445 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 398 617 2106 2285 2386 2396 3009
Size: 211 187 219 1489 179 101 10 613
Fragments arranged by size:
1489 613 219 211 187 179 101 10
Bpu10I CC'TnA_GC
Cuts at: 0 163 1053 1405 1568 1718 2206 2457 2499
Size: 163 890 352 163 150 488 251 42
Cuts at: 2499 2537 3009
Size: 38 472
Fragments arranged by size:
890 488 472 352 251 163 163 150
42 38
BsaI GGTCTCn'nnnn_
Cuts at: 0 346 1964 2184 3009
Size: 346 1618 220 825
Fragments arranged by size:
1618 825 346 220
BsaAI yAC'GTr
Cuts at: 0 2686 3009
Size: 2686 323
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 792 822 1208 1238 2384 2414 3009
Size: 792 30 386 30 1146 30 595
Fragments arranged by size:
1146 792 595 386 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 151 295 635 722 741 744 764 1056
Size: 151 144 340 87 19 3 20 292
Cuts at: 1056 1310 1326 1715 2148 2320 2339 2597 3006
Size: 254 16 389 433 172 19 258 409
Cuts at: 3006 3009
Size: 3
Fragments arranged by size:
433 409 389 340 292 258 254 172
151 144 87 20 19 19 16 3
3
BseYI C'CCAG_C
Cuts at: 0 37 259 1012 1245 1352 1462 1561 1852
Size: 37 222 753 233 107 110 99 291
Cuts at: 1852 1903 2078 2217 2232 2275 2920 3009
Size: 51 175 139 15 43 645 89
Fragments arranged by size:
753 645 291 233 222 175 139 110
107 99 89 51 43 37 15
BsiHKAI G_wGCw'C
Cuts at: 0 104 2349 3009
Size: 104 2245 660
Fragments arranged by size:
2245 660 104
Bsp1286I G_dGCh'C
Cuts at: 0 46 104 394 425 716 730 908 921
Size: 46 58 290 31 291 14 178 13
Cuts at: 921 945 1044 1173 1348 1425 1724 1897 2100
Size: 24 99 129 175 77 299 173 203
Cuts at: 2100 2271 2349 2452 2777 2801 2825 2964 3009
Size: 171 78 103 325 24 24 139 45
Fragments arranged by size:
325 299 291 290 203 178 175 173
171 139 129 103 99 78 77 58
46 45 31 24 24 24 14 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 184 367 660 896 3009
Size: 184 183 293 236 2113
Fragments arranged by size:
2113 293 236 184 183
BsrDI GCAATG_nn'
Cuts at: 0 18 627 1660 3009
Size: 18 609 1033 1349
Fragments arranged by size:
1349 1033 609 18
BsrFI r'CCGG_y
Cuts at: 0 308 2645 3009
Size: 308 2337 364
Fragments arranged by size:
2337 364 308
BstEII G'GTnAC_C
Cuts at: 0 243 2236 3009
Size: 243 1993 773
Fragments arranged by size:
1993 773 243
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1279 2232 3009
Size: 1279 953 777
Fragments arranged by size:
1279 953 777
BstYI r'GATC_y
Cuts at: 0 700 1918 2055 2405 2781 3009
Size: 700 1218 137 350 376 228
Fragments arranged by size:
1218 700 376 350 228 137
Bsu36I CC'TnA_GG
Cuts at: 0 1338 3009
Size: 1338 1671
BtgI C'CryG_G
Cuts at: 0 923 2132 2511 3009
Size: 923 1209 379 498
Fragments arranged by size:
1209 923 498 379
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 182 3009
Size: 182 2827
BtsI GCAGTG_nn'
Cuts at: 0 27 1416 3009
Size: 27 1389 1593
Fragments arranged by size:
1593 1389 27
DraI TTT'AAA
Cuts at: 0 2027 3009
Size: 2027 982
DraIII CAC_nnn'GTG
Cuts at: 0 411 1588 2689 3009
Size: 411 1177 1101 320
Fragments arranged by size:
1177 1101 411 320
DrdI GACnn_nn'nnGTC
Cuts at: 0 1160 2530 3009
Size: 1160 1370 479
Fragments arranged by size:
1370 1160 479
EaeI y'GGCC_r
Cuts at: 0 50 980 2310 2658 2853 2970 3009
Size: 50 930 1330 348 195 117 39
Fragments arranged by size:
1330 930 348 195 117 50 39
EarI CTCTTCn'nnn_
Cuts at: 0 375 784 2306 3009
Size: 375 409 1522 703
Fragments arranged by size:
1522 703 409 375
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 327 3009
Size: 327 2682
EcoICRI GAG'CTC
Cuts at: 0 2347 3009
Size: 2347 662
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 398 617 749 857 1796 1826 2106
Size: 211 187 219 132 108 939 30 280
Cuts at: 2106 2285 2386 2396 2780 3009
Size: 179 101 10 384 229
Fragments arranged by size:
939 384 280 229 219 211 187 179
132 108 101 30 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2362 3009
Size: 2362 647
EcoO109I rG'GnC_Cy
Cuts at: 0 42 43 173 421 422 478 538 904
Size: 42 1 130 248 1 56 60 366
Cuts at: 904 905 917 918 934 1034 1138 1170 1575
Size: 1 12 1 16 100 104 32 405
Cuts at: 1575 1616 1744 1780 1894 2153 2172 2714 2798
Size: 41 128 36 114 259 19 542 84
Cuts at: 2798 2811 3009
Size: 13 198
Fragments arranged by size:
542 405 366 259 248 198 130 128
114 104 100 84 60 56 42 41
36 32 19 16 13 12 1 1
1 1
HaeII r_GCGC'y
Cuts at: 0 81 3009
Size: 81 2928
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 712 744 813 845 2047 2079 2654 2686
Size: 712 32 69 32 1202 32 575 32
Cuts at: 2686 2927 2959 3009
Size: 241 32 50
Fragments arranged by size:
1202 712 575 241 69 50 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 1284 3009
Size: 1284 1725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2204 3009
Size: 2204 805
MscI TGG'CCA
Cuts at: 0 52 2312 3009
Size: 52 2260 697
Fragments arranged by size:
2260 697 52
MslI CAynn'nnrTG
Cuts at: 0 788 1277 3009
Size: 788 489 1732
Fragments arranged by size:
1732 788 489
MspA1I CmG'CkG
Cuts at: 0 979 1438 1903 2941 3009
Size: 979 459 465 1038 68
Fragments arranged by size:
1038 979 465 459 68
NaeI GCC'GGC
Cuts at: 0 2647 3009
Size: 2647 362
NcoI C'CATG_G
Cuts at: 0 2511 3009
Size: 2511 498
NgoMIV G'CCGG_C
Cuts at: 0 2645 3009
Size: 2645 364
NheI G'CTAG_C
Cuts at: 0 958 3009
Size: 958 2051
PasI CC'CwG_GG
Cuts at: 0 418 541 909 1603 2335 3009
Size: 418 123 368 694 732 674
Fragments arranged by size:
732 694 674 418 368 123
PflMI CCAn_nnn'nTGG
Cuts at: 0 71 472 2512 3009
Size: 71 401 2040 497
Fragments arranged by size:
2040 497 401 71
PfoI T'CCnGG_A
Cuts at: 0 375 1202 3009
Size: 375 827 1807
Fragments arranged by size:
1807 827 375
PmlI CAC'GTG
Cuts at: 0 2686 3009
Size: 2686 323
PpuMI rG'GwC_Cy
Cuts at: 0 173 478 1744 2714 2811 3009
Size: 173 305 1266 970 97 198
Fragments arranged by size:
1266 970 305 198 173 97
PspOMI G'GGCC_C
Cuts at: 0 42 421 904 917 1169 1893 2797 2960
Size: 42 379 483 13 252 724 904 163
Cuts at: 2960 3009
Size: 49
Fragments arranged by size:
904 724 483 379 252 163 49 42
13
PstI C_TGCA'G
Cuts at: 0 978 1926 2327 3009
Size: 978 948 401 682
Fragments arranged by size:
978 948 682 401
PvuII CAG'CTG
Cuts at: 0 1438 1903 2941 3009
Size: 1438 465 1038 68
Fragments arranged by size:
1438 1038 465 68
SacI G_AGCT'C
Cuts at: 0 2349 3009
Size: 2349 660
SexAI A'CCwGG_T
Cuts at: 0 1746 3009
Size: 1746 1263
SfcI C'TryA_G
Cuts at: 0 786 974 1922 2323 2392 2927 3009
Size: 786 188 948 401 69 535 82
Fragments arranged by size:
948 786 535 401 188 82 69
SgrAI Cr'CCGG_yG
Cuts at: 0 308 3009
Size: 308 2701
SmaI CCC'GGG
Cuts at: 0 251 2150 2626 2796 2828 2959 3009
Size: 251 1899 476 170 32 131 50
Fragments arranged by size:
1899 476 251 170 131 50 32
SmlI C'TyrA_G
Cuts at: 0 2815 3009
Size: 2815 194
StuI AGG'CCT
Cuts at: 0 520 2484 3009
Size: 520 1964 525
Fragments arranged by size:
1964 525 520
StyI C'CwwG_G
Cuts at: 0 137 212 559 613 983 2511 2777 2912
Size: 137 75 347 54 370 1528 266 135
Cuts at: 2912 3009
Size: 97
Fragments arranged by size:
1528 370 347 266 137 135 97 75
54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2209 2224 3009
Size: 2209 15 785
Fragments arranged by size:
2209 785 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 191 223 397 429 935 967 1380 1412
Size: 191 32 174 32 506 32 413 32
Cuts at: 1412 2040 2072 2673 2705 3009
Size: 628 32 601 32 304
Fragments arranged by size:
628 601 506 413 304 191 174 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 574 1748 3009
Size: 574 1174 1261
Fragments arranged by size:
1261 1174 574
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1678 3009
Size: 1678 1331
XmaI C'CCGG_G
Cuts at: 0 249 2148 2624 2794 2826 2957 3009
Size: 249 1899 476 170 32 131 52
Fragments arranged by size:
1899 476 249 170 131 52 32
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AflIII AleI AlwNI
ApaI ApoI AseI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BglI BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BsaI BsaAI BsaXI BseRI BseYI BsiHKAI
Bsp1286I BspMI BsrDI BsrFI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NheI
PasI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII
SacI SexAI SfcI SgrAI SmaI SmlI StuI StyI
TaqII TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AgeI AhdI AlfI AloI ApaLI
AscI AsiSI AvrII BaeI BbeI BciVI BclI BfrBI
BglII BmgBI BpuEI BsaBI BsaHI BsaWI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII
BssSI BstAPI BstBI BstZ17I ClaI CspCI EagI EcoRI
EcoRV FalI FseI FspI FspAI HincII HpaI KasI
KpnI MfeI MluI NarI NdeI NotI NruI NsiI
NspI PacI PciI PmeI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SnaBI SpeI SphI SrfI
SspI SwaI TatI XbaI XhoI XmnI ZraI