(Circular) (Six-base) MAPSORT of: pFN21AA1849.seq Check: 6816 from: 1 to: 7924 pFN21AA1849 7924 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3868 4404 3868 Size: 536 7388 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5734 278 Size: 53 83 186 936 4198 2468 Fragments arranged by size: 4198 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3711 5267 1937 Size: 1774 1556 4594 Fragments arranged by size: 4594 1774 1556 Acc65I G'GTAC_C Cuts at: 5245 5245 Size: 7924 AclI AA'CG_TT Cuts at: 1563 6051 6424 1563 Size: 4488 373 3063 Fragments arranged by size: 4488 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2075 2219 4264 5987 7371 7639 1632 Size: 443 144 2045 1723 1384 268 1917 Fragments arranged by size: 2045 1917 1723 1384 443 268 144 AfeI AGC'GCT Cuts at: 4785 4785 Size: 7924 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7727 AflIII A'CryG_T Cuts at: 1203 6823 7800 1203 Size: 5620 977 1327 Fragments arranged by size: 5620 1327 977 AgeI A'CCGG_T Cuts at: 6755 6755 Size: 7924 AhdI GACnn_n'nnGTC Cuts at: 6653 6653 Size: 7924 AleI CACnn'nnGTG Cuts at: 1166 2790 1166 Size: 1624 6300 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7924 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3614 3646 3614 Size: 32 7892 AlwNI CAG_nnn'CTG Cuts at: 2220 3088 3211 3376 4030 4396 5020 5667 7239 Size: 868 123 165 654 366 624 647 1572 Cuts at: 7239 2220 Size: 2905 Fragments arranged by size: 2905 1572 868 654 647 624 366 165 123 ApaI G_GGCC'C Cuts at: 2702 3163 3968 4126 4235 4261 2702 Size: 461 805 158 109 26 6365 Fragments arranged by size: 6365 805 461 158 109 26 ApaLI G'TGCA_C Cuts at: 3881 5981 7137 3881 Size: 2100 1156 4668 Fragments arranged by size: 4668 2100 1156 ApoI r'AATT_y Cuts at: 1493 5233 5459 5611 7720 1493 Size: 3740 226 152 2109 1697 Fragments arranged by size: 3740 2109 1697 226 152 AscI GG'CGCG_CC Cuts at: 3107 3107 Size: 7924 AseI AT'TA_AT Cuts at: 160 1032 6478 160 Size: 872 5446 1606 Fragments arranged by size: 5446 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7924 AvaI C'yCGr_G Cuts at: 1870 1943 2451 2702 2960 3096 3155 3162 3815 Size: 73 508 251 258 136 59 7 653 Cuts at: 3815 3887 4271 4493 4537 4578 5000 5026 5249 Size: 72 384 222 44 41 422 26 223 Cuts at: 5249 1870 Size: 4545 Fragments arranged by size: 4545 653 508 422 384 258 251 223 222 136 73 72 59 44 41 26 7 BamHI G'GATC_C Cuts at: 5254 5621 5254 Size: 367 7557 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2144 2258 3201 4524 4994 Size: 325 217 627 357 114 943 1323 470 Cuts at: 4994 5052 5245 6700 618 Size: 58 193 1455 1842 Fragments arranged by size: 1842 1455 1323 943 627 470 357 325 217 193 114 58 BanII G_rGCy'C Cuts at: 1391 2169 2621 2669 2702 2712 2955 3045 3163 Size: 778 452 48 33 10 243 90 118 Cuts at: 3163 3231 3462 3546 3968 4126 4181 4235 4261 Size: 68 231 84 422 158 55 54 26 Cuts at: 4261 4621 4667 5007 5243 1391 Size: 360 46 340 236 4072 Fragments arranged by size: 4072 778 452 422 360 340 243 236 231 158 118 90 84 68 55 54 48 46 33 26 10 BbeI G_GCGC'C Cuts at: 2148 3205 4998 5056 2148 Size: 1057 1793 58 5016 Fragments arranged by size: 5016 1793 1057 58 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4912 928 Size: 971 3013 3940 Fragments arranged by size: 3940 3013 971 BbvCI CC'TCA_GC Cuts at: 2480 2480 Size: 7924 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2555 2589 6115 6149 1903 Size: 34 618 34 3526 34 3678 Fragments arranged by size: 3678 3526 618 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5084 5816 7026 5084 Size: 732 1210 5982 Fragments arranged by size: 5982 1210 732 BclI T'GATC_A Cuts at: 1548 1548 Size: 7924 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2361 4157 4662 Size: 107 122 71 631 605 689 1796 505 Cuts at: 4662 5103 6535 136 Size: 441 1432 1525 Fragments arranged by size: 1796 1525 1432 689 631 605 505 441 122 107 71 BglII A'GATC_T Cuts at: 7919 7919 Size: 7924 BlpI GC'TnA_GC Cuts at: 2099 5341 2099 Size: 3242 4682 Bme1580I G_kGCm'C Cuts at: 1790 2702 3000 3163 3362 3764 3885 3968 4126 Size: 912 298 163 199 402 121 83 158 Cuts at: 4126 4235 4261 4493 4527 4630 5985 7141 1790 Size: 109 26 232 34 103 1355 1156 2573 Fragments arranged by size: 2573 1355 1156 912 402 298 232 199 163 158 121 109 103 83 34 26 BmgBI CAC'GTC Cuts at: 1318 2347 2497 5182 1318 Size: 1029 150 2685 4060 Fragments arranged by size: 4060 2685 1029 150 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2663 5655 6613 Size: 416 53 83 399 24 1246 2992 958 Cuts at: 6613 442 Size: 1753 Fragments arranged by size: 2992 1753 1246 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7924 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2640 3481 3913 4635 2640 Size: 841 432 722 5929 Fragments arranged by size: 5929 841 722 432 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2119 2248 2315 2824 2969 3239 3257 3437 Size: 880 129 67 509 145 270 18 180 Cuts at: 3437 3695 3776 4052 4086 4319 4550 4857 4879 Size: 258 81 276 34 233 231 307 22 Cuts at: 4879 5099 6584 1239 Size: 220 1485 2579 Fragments arranged by size: 2579 1485 880 509 307 276 270 258 233 231 220 180 145 129 81 67 34 22 18 Bpu10I CC'TnA_GC Cuts at: 2480 2663 3974 4734 2480 Size: 183 1311 760 5670 Fragments arranged by size: 5670 1311 760 183 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3365 4630 6049 6914 7212 3365 Size: 1265 1419 865 298 4077 Fragments arranged by size: 4077 1419 1265 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2648 6587 882 Size: 617 1149 3939 2219 Fragments arranged by size: 3939 2219 1149 617 BsaAI yAC'GTr Cuts at: 493 1204 2335 4510 5715 493 Size: 711 1131 2175 1205 2702 Fragments arranged by size: 2702 2175 1205 1131 711 BsaBI GATnn'nnATC Cuts at: 7918 7918 Size: 7924 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2145 3202 4995 5053 Size: 53 83 186 936 612 1057 1793 58 Cuts at: 5053 5731 6113 6748 275 Size: 678 382 635 1451 Fragments arranged by size: 1793 1451 1057 936 678 635 612 382 186 83 58 53 BsaWI w'CCGG_w Cuts at: 4834 6357 6755 7029 7176 7587 4834 Size: 1523 398 274 147 411 5171 Fragments arranged by size: 5171 1523 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2108 2138 3017 3047 3704 Size: 30 165 30 705 30 879 30 657 Cuts at: 3704 3734 1178 Size: 30 5368 Fragments arranged by size: 5368 879 705 657 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2516 2613 2720 3608 3671 3692 3833 3944 Size: 1330 97 107 888 63 21 141 111 Cuts at: 3944 4877 1186 Size: 933 4233 Fragments arranged by size: 4233 1330 933 888 141 111 107 97 63 21 BseYI C'CCAG_C Cuts at: 2175 2369 2849 3081 4125 4180 4334 4479 4570 Size: 194 480 232 1044 55 154 145 91 Cuts at: 4570 4715 4954 5062 7127 7623 2175 Size: 145 239 108 2065 496 2476 Fragments arranged by size: 2476 2065 1044 496 480 239 232 194 154 145 145 108 91 55 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2206 2875 2999 3622 4078 4175 4848 Size: 573 470 669 124 623 456 97 673 Cuts at: 4848 1163 Size: 4239 Fragments arranged by size: 4239 673 669 623 573 470 456 124 97 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4657 5046 5374 6135 7163 1540 Size: 275 186 2656 389 328 761 1028 2301 Fragments arranged by size: 2656 2301 1028 761 389 328 275 186 BsiHKAI G_wGCw'C Cuts at: 2169 2621 2803 3546 3885 4074 4244 5985 6070 Size: 452 182 743 339 189 170 1741 85 Cuts at: 6070 7141 2169 Size: 1071 2952 Fragments arranged by size: 2952 1741 1071 743 452 339 189 182 170 85 BsmI GAATG_Cn' Cuts at: 5435 5528 5435 Size: 93 7831 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2169 2621 2669 2702 2712 2803 2955 Size: 399 379 452 48 33 10 91 152 Cuts at: 2955 3000 3045 3163 3231 3362 3462 3546 3764 Size: 45 45 118 68 131 100 84 218 Cuts at: 3764 3885 3968 4074 4126 4181 4235 4244 4261 Size: 121 83 106 52 55 54 9 17 Cuts at: 4261 4493 4527 4621 4630 4667 5007 5243 5985 Size: 232 34 94 9 37 340 236 742 Cuts at: 5985 6070 7141 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 452 399 379 340 236 232 218 152 131 121 118 106 100 94 91 85 84 83 68 55 54 52 48 45 45 37 34 33 17 10 9 9 BspEI T'CCGG_A Cuts at: 4834 7587 4834 Size: 2753 5171 BspHI T'CATG_A Cuts at: 3261 5813 7543 3261 Size: 2552 1730 3642 Fragments arranged by size: 3642 2552 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3036 3060 3868 4113 4404 5279 844 Size: 2192 24 808 245 291 875 3489 Fragments arranged by size: 3489 2192 875 808 291 245 24 BsrBI CCG'CTC Cuts at: 1394 1823 2299 2911 5811 1394 Size: 429 476 612 2900 3507 Fragments arranged by size: 3507 2900 612 476 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6419 6593 65 Size: 1183 5171 174 1396 Fragments arranged by size: 5171 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2255 2324 2360 2812 2901 3135 4049 Size: 110 445 69 36 452 89 234 914 Cuts at: 4049 4194 6568 6755 1700 Size: 145 2374 187 2869 Fragments arranged by size: 2869 2374 914 452 445 234 187 145 110 89 69 36 BsrGI T'GTAC_A Cuts at: 96 3732 96 Size: 3636 4288 BssHII G'CGCG_C Cuts at: 1925 3107 1925 Size: 1182 6742 BssSI C'ACGA_G Cuts at: 5984 6996 5984 Size: 1012 6912 BstAPI GCAn_nnn'nTGC Cuts at: 794 3376 4198 4856 794 Size: 2582 822 658 3862 Fragments arranged by size: 3862 2582 822 658 BstBI TT'CG_AA Cuts at: 6728 6728 Size: 7924 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7924 BstXI CCAn_nnnn'nTGG Cuts at: 2521 3469 4372 2521 Size: 948 903 6073 Fragments arranged by size: 6073 948 903 BstYI r'GATC_y Cuts at: 1972 5199 5254 5621 6006 6023 7475 7561 7573 Size: 3227 55 367 385 17 1452 86 12 Cuts at: 7573 7919 1972 Size: 346 1977 Fragments arranged by size: 3227 1977 1452 385 367 346 86 55 17 12 BtgI C'CryG_G Cuts at: 513 1065 2931 3006 3019 3483 3663 4227 4657 Size: 552 1866 75 13 464 180 564 430 Cuts at: 4657 513 Size: 3780 Fragments arranged by size: 3780 1866 564 552 464 430 180 75 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2166 2379 487 Size: 683 56 940 213 6032 Fragments arranged by size: 6032 940 683 213 56 BtsI GCAGTG_nn' Cuts at: 2887 3710 3935 4474 5440 6234 6254 2887 Size: 823 225 539 966 794 20 4557 Fragments arranged by size: 4557 966 823 794 539 225 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7924 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2616 2651 529 Size: 35 2052 35 5802 Fragments arranged by size: 5802 2052 35 35 DraI TTT'AAA Cuts at: 5228 5228 Size: 7924 DraIII CAC_nnn'GTG Cuts at: 2822 4510 2822 Size: 1688 6236 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6931 809 Size: 507 5615 1802 Fragments arranged by size: 5615 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2250 2326 2923 Size: 54 1425 50 275 18 420 76 597 Cuts at: 2923 3339 3471 3648 4110 4224 4654 4754 5043 Size: 416 132 177 462 114 430 100 289 Cuts at: 5043 5371 6260 7889 8 Size: 328 889 1629 43 Fragments arranged by size: 1629 1425 889 597 462 430 420 416 328 289 275 177 132 114 100 76 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4654 5043 5371 1537 Size: 275 2842 389 328 4090 Fragments arranged by size: 4090 2842 389 328 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2480 3923 4608 5854 1353 Size: 1127 1443 685 1246 3423 Fragments arranged by size: 3423 1443 1246 1127 685 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2888 4397 6511 6885 7031 387 Size: 1064 138 1299 1509 2114 374 146 1280 Fragments arranged by size: 2114 1509 1299 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2167 2619 3544 2167 Size: 452 925 6547 Fragments arranged by size: 6547 925 452 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 2119 2219 2248 2315 2824 2969 Size: 393 443 44 100 29 67 509 145 Cuts at: 2969 3239 3257 3437 3695 3776 4052 4086 4264 Size: 270 18 180 258 81 276 34 178 Cuts at: 4264 4319 4550 4857 4879 5099 5987 6584 7371 Size: 55 231 307 22 220 888 597 787 Cuts at: 7371 7639 1239 Size: 268 1524 Fragments arranged by size: 1524 888 787 597 509 443 393 307 276 270 268 258 231 220 180 178 145 100 81 67 55 44 34 29 22 18 EcoNI CCTnn'n_nnAGG Cuts at: 2765 4859 2765 Size: 2094 5830 EcoO109I rG'GnC_Cy Cuts at: 2116 2698 2699 3025 3067 3159 3964 3965 4122 Size: 582 1 326 42 92 805 1 157 Cuts at: 4122 4232 4257 4473 4534 4561 4633 4744 4943 Size: 110 25 216 61 27 72 111 199 Cuts at: 4943 2116 Size: 5097 Fragments arranged by size: 5097 805 582 326 216 199 157 111 110 92 72 61 42 27 25 1 1 EcoRI G'AATT_C Cuts at: 5233 5611 5233 Size: 378 7546 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2443 2443 Size: 7924 FseI GG_CCGG'CC Cuts at: 2328 2328 Size: 7924 FspI TGC'GCA Cuts at: 6430 6430 Size: 7924 HaeII r_GCGC'y Cuts at: 1678 2148 2271 2294 3205 3784 4050 4787 4998 Size: 470 123 23 911 579 266 737 211 Cuts at: 4998 5056 7071 1678 Size: 58 2015 2531 Fragments arranged by size: 2531 2015 911 737 579 470 266 211 123 58 23 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2108 2140 3017 3049 3779 Size: 32 135 32 536 32 877 32 730 Cuts at: 3779 3811 6565 6597 6639 6671 1373 Size: 32 2754 32 42 32 2626 Fragments arranged by size: 2754 2626 877 730 536 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2980 5268 5514 669 Size: 988 281 1042 2288 246 3079 Fragments arranged by size: 3079 2288 1042 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7924 HpaI GTT'AAC Cuts at: 5514 5514 Size: 7924 KasI G'GCGC_C Cuts at: 2144 3201 4994 5052 2144 Size: 1057 1793 58 5016 Fragments arranged by size: 5016 1793 1057 58 KpnI G_GTAC'C Cuts at: 5249 5249 Size: 7924 MfeI C'AATT_G Cuts at: 5523 5523 Size: 7924 MluI A'CGCG_T Cuts at: 7800 7800 Size: 7924 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2472 5202 7038 7222 1305 Size: 1167 2730 1836 184 2007 Fragments arranged by size: 2730 2007 1836 1167 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2252 3473 4112 4756 10 Size: 54 1425 343 420 1221 639 644 3178 Fragments arranged by size: 3178 1425 1221 644 639 420 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2790 3668 4136 5882 6241 6400 518 Size: 648 1624 878 468 1746 359 159 2042 Fragments arranged by size: 2042 1746 1624 878 648 468 359 159 MspA1I CmG'CkG Cuts at: 1606 2071 2179 2377 2569 2687 2831 2922 2933 Size: 465 108 198 192 118 144 91 11 Cuts at: 2933 3211 3477 3601 3874 4189 4216 4815 5042 Size: 278 266 124 273 315 27 599 227 Cuts at: 5042 5340 6017 7165 7410 1606 Size: 298 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 599 465 315 298 278 273 266 245 227 198 192 144 124 118 108 91 27 11 NaeI GCC'GGC Cuts at: 2326 2362 2903 3137 4051 2326 Size: 36 541 234 914 6199 Fragments arranged by size: 6199 914 541 234 36 NarI GG'CG_CC Cuts at: 2145 3202 4995 5053 2145 Size: 1057 1793 58 5016 Fragments arranged by size: 5016 1793 1057 58 NcoI C'CATG_G Cuts at: 513 1065 3483 3663 4227 513 Size: 552 2418 180 564 4210 Fragments arranged by size: 4210 2418 564 552 180 NdeI CA'TA_TG Cuts at: 387 387 Size: 7924 NgoMIV G'CCGG_C Cuts at: 2324 2360 2901 3135 4049 2324 Size: 36 541 234 914 6199 Fragments arranged by size: 6199 914 541 234 36 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7924 NotI GC'GGCC_GC Cuts at: 5371 5371 Size: 7924 NruI TCG'CGA Cuts at: 5171 5171 Size: 7924 NspI r_CATG'y Cuts at: 1132 1229 2086 3583 5282 6827 1132 Size: 97 857 1497 1699 1545 2229 Fragments arranged by size: 2229 1699 1545 1497 857 97 PasI CC'CwG_GG Cuts at: 1348 2602 2695 3037 4119 4557 4623 1348 Size: 1254 93 342 1082 438 66 4649 Fragments arranged by size: 4649 1254 1082 438 342 93 66 PciI A'CATG_T Cuts at: 6823 6823 Size: 7924 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7924 PfoI T'CCnGG_A Cuts at: 2090 2433 2465 2913 2946 3216 3453 3753 3929 Size: 343 32 448 33 270 237 300 176 Cuts at: 3929 4029 5076 2090 Size: 100 1047 4938 Fragments arranged by size: 4938 1047 448 343 300 270 237 176 100 33 32 PmeI GTTT'AAAC Cuts at: 5228 5228 Size: 7924 PmlI CAC'GTG Cuts at: 4510 4510 Size: 7924 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5993 6025 7506 7538 5993 Size: 32 1481 32 6379 Fragments arranged by size: 6379 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2116 3025 3067 4561 4744 2116 Size: 909 42 1494 183 5296 Fragments arranged by size: 5296 1494 909 183 42 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7924 PsiI TTA'TAA Cuts at: 5494 5494 Size: 7924 PspOMI G'GGCC_C Cuts at: 2698 3159 3964 4122 4231 4257 2698 Size: 461 805 158 109 26 6365 Fragments arranged by size: 6365 805 461 158 109 26 PstI C_TGCA'G Cuts at: 830 2568 2769 3255 3879 4023 4852 5276 830 Size: 1738 201 486 624 144 829 424 3478 Fragments arranged by size: 3478 1738 829 624 486 424 201 144 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7924 PvuII CAG'CTG Cuts at: 2071 2179 3211 3477 3601 3874 4189 4216 2071 Size: 108 1032 266 124 273 315 27 5779 Fragments arranged by size: 5779 1032 315 273 266 124 108 27 SacI G_AGCT'C Cuts at: 2169 2621 3546 2169 Size: 452 925 6547 Fragments arranged by size: 6547 925 452 SacII CC_GC'GG Cuts at: 2934 2934 Size: 7924 SalI G'TCGA_C Cuts at: 1936 5266 1936 Size: 3330 4594 SanDI GG'GwC_CC Cuts at: 4561 4744 4561 Size: 183 7741 SbfI CC_TGCA'GG Cuts at: 5276 5276 Size: 7924 ScaI AGT'ACT Cuts at: 6172 7884 6172 Size: 1712 6212 SfcI C'TryA_G Cuts at: 826 1045 2564 2765 2773 3251 3875 4019 4848 Size: 219 1519 201 8 478 624 144 829 Cuts at: 4848 5272 6407 7088 7279 826 Size: 424 1135 681 191 1471 Fragments arranged by size: 1519 1471 1135 829 681 624 478 424 219 201 191 144 8 SfoI GGC'GCC Cuts at: 2146 3203 4996 5054 2146 Size: 1057 1793 58 5016 Fragments arranged by size: 5016 1793 1057 58 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7924 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7924 SmaI CCC'GGG Cuts at: 1872 2962 3098 3164 4580 5002 5028 5251 1872 Size: 1090 136 66 1416 422 26 223 4545 Fragments arranged by size: 4545 1416 1090 422 223 136 66 26 SmlI C'TyrA_G Cuts at: 820 1017 1943 3380 4609 6028 6929 7191 820 Size: 197 926 1437 1229 1419 901 262 1553 Fragments arranged by size: 1553 1437 1419 1229 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5715 493 Size: 5222 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7924 SphI G_CATG'C Cuts at: 1132 2086 5282 1132 Size: 954 3196 3774 Fragments arranged by size: 3774 3196 954 SrfI GCCC'GGGC Cuts at: 3098 3098 Size: 7924 SspI AAT'ATT Cuts at: 5 52 5848 5 Size: 47 5796 2081 Fragments arranged by size: 5796 2081 47 StuI AGG'CCT Cuts at: 1517 4007 4277 4462 1517 Size: 2490 270 185 4979 Fragments arranged by size: 4979 2490 270 185 StyI C'CwwG_G Cuts at: 513 1065 2514 3102 3483 3663 4227 5107 5363 Size: 552 1449 588 381 180 564 880 256 Cuts at: 5363 513 Size: 3074 Fragments arranged by size: 3074 1449 880 588 564 552 381 256 180 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2189 3359 4132 4336 5951 5968 6121 Size: 1255 609 1170 773 204 1615 17 153 Cuts at: 6121 6306 6751 325 Size: 185 445 1498 Fragments arranged by size: 1615 1498 1255 1170 773 609 445 204 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3549 3732 6170 Size: 274 80 33 51 1443 1572 183 2438 Cuts at: 6170 7882 96 Size: 1712 138 Fragments arranged by size: 2438 1712 1572 1443 274 183 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4311 4343 7884 7916 4311 Size: 32 3541 32 4319 Fragments arranged by size: 4319 3541 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2216 1316 Size: 900 7024 XbaI T'CTAG_A Cuts at: 5260 5260 Size: 7924 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2786 3481 3490 3670 2786 Size: 695 9 180 7040 Fragments arranged by size: 7040 695 180 9 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7924 XmaI C'CCGG_G Cuts at: 1870 2960 3096 3162 4578 5000 5026 5249 1870 Size: 1090 136 66 1416 422 26 223 4545 Fragments arranged by size: 4545 1416 1090 422 223 136 66 26 XmnI GAAnn'nnTTC Cuts at: 6053 6053 Size: 7924 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5732 276 Size: 53 83 186 936 4198 2468 Fragments arranged by size: 4198 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoRV FspAI NsiI PacI PspXI PsrI RsrII SapI SexAI SfiI SwaI